• Food Science of Animal Resources
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• v.44 no.4
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• pp.912-933
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• 2024

The objective of this study was to isolate, identify, and evaluate novel Korean starter cultures for use in fermented sausages. A total of 72 strains were isolated from various indigenous sources, including Nuruk, Jeotgal, and mudflats on the west coast of South Korea. Two strains were identified as Penicillium nalgiovense (SD01 and SJ02), a traditional starter used in the production of fermented sausages. A comparative analysis was performed between SD01 and SJ02 using the commercial starter culture (M600). Strain SJ02 exhibited superior lipolytic and proteolytic activities, as well as an enhanced growth rate at the optimal salinity level of 2% NaCl compared to M600. No significant differences were observed in thiobarbituric acid reactive substances values, sausage colors, and texture properties between SJ02 and M600 fermented sausages, except for adhesiveness. Profiles of mycotoxin-related genes were similar for both strains. Electronic nose analysis revealed distinct aroma profiles between SJ02 and M600 fermented sausages, with a relatively higher levels of propan-2-one and butyl butanoate in SJ02, and a higher level of ethanol and propanal in M600. In electronic tongue analysis, there was no significant differences in taste characteristics between SJ02 and M600. These results indicate that P. nalgiovense SJ02 is a potential starter culture to produce dry fermented sausages, enhancing Korean style cured meat processing industry.

• Proceedings of the IEEK Conference
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• 2002.06b
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• pp.181-184
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• 2002

본 논문에서는 600Hz 무선 LAN 시스템에 탑재를 위한 600㎓ 대역 전력증폭기 모듈을 개발 하였다. 600㎓ 대역 전력증폭기 모듈에 실장된 600㎓ 대역 전력증폭기 MMIC는 ETRI에서 설계 및 제작한 것으로 칩의 크기는 2.80 × 1.75㎟이며, on-wafer측정을 하여 얻은 결과는 동작 주파수 58~620Hz에서 소신호 이득은 12.4dB이고, 최대 소신호 이득은 59~60G보z에서 ISdB이며, 출력전력(Pldn)은 16.3~16.7dBm을 얻었다. 이와 같은 특성을 갖는 전력증폭기 MMIC를 사용하여 모듈을 제작하였으며, RF feed line을 위해 Rogers 사의 R03003 기판을 사용하였다. 모듈의 입출력은 동작 주파수 대역에 적합한 WRl5라는 waveguide 형태를 사용하였고, DC 바이어스 공급을 위해 3.5㎜ K-connector를 사용하였다 제작한 모듈의 크기는 40 × 30 × 15㎣이며, 최적의 성능을 얻고자 tuning bar를 상하로 이동하여 최적점을 찾았으며 나사로 고정하여 상태를 유지하도록 하였다. DC 바이어스 및 RF feed line과 칩의 연결은 본딩에 의한 인덕턴스를 최소화하기 위하여 3mil 두께의 리본 본딩을 하였다 전력증폭기 모듈을 측정한 결과, 동작주파수 600㎓ 대에서 소신호 이득은 6dB 이상, 입력 정합은 -lOdB 이하, 출력 정합은 -4dB 이하로 측정되었긴, 출력전력은 SdBm 이상으로 측정되었다. 동국대에서 제작한 600Hz 무선 LAN 시스템에 전력증폭기 모듈을 시스템 송신부에 탑재 시험한 결과, 동영상을 실시간으로 전송하는데 성공하였다.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.2
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• pp.95-102
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• 2017

This paper describes the experimental results for the structural performance of full-scale coupling beams with different reinforcement layout (diagonal and horizontal). For the reinforcements of the coupling beams, high-strength steel bars(SD500 and SD600) were used in order to improve workability and economic feasibility. The rigid steel frames and linked joints were used to maintain the clear span length (distance between both shear walls) of the coupling beam during the cyclic loading. Experimental results indicated that the diagonally reinforced coupling beam specimen could exhibit more ductile behavior compared to horizontally reinforced specimen. ACI318-14 code is applicable to design of coupling beam with diagonally reinforcement, however, that is overestimating the strength of horizontally reinforced coupling beam. It is remarkable that effective elastic stiffness values of both reinforcement details coupling beam significantly lees than ASCE 41-13.

• BMB Reports
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• v.43 no.6
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• pp.407-412
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• 2010

Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd. To access whether phosphorylation sites within HD and NK2-SD affect the interaction of the protein with the target sequence, alanine substitutions were introduced. The result shows that S631A mutation within NK2-SD does not contribute significantly to the DNA-binding affinity. However, S571A and T600A mutations within HD showed lower affinity for DNA binding. In addition, DNA-binding analysis using embryonic nuclear protein also demonstrates that Vnd interacts with other nuclear proteins, suggesting the existence of Vnd as a complex.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.600-604
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• 2007

In this study, we first prepared 3 kinds of powders with different molecular masses from the crude protein-bound polysaccharide extract of Agraricus blazei Murill through ultrafiltration, followed by spray-drying. Then, the antitumor activities of the powders were analyzed. Size exclusion chromatography coupled with a multi-angle laser-light-scattering system showed the 3 powders had the following molecular ranges: below 10 kDa (SD-1), 10 to 150 kDa (SD-2), and above 150 kDa (SD-3), representing peak molecular weights of $8.26{\times}10^3,\;9.65{\times}10^4$, and $5.94{\times}10^6\;g/mol$, respectively. All the powders stimulated macrophage RAW264.7 cells to produce nitric oxide, of which SD-2 and SD-3 were superior to the crude extract powder (CP-SD), while SD-1 showed the lowest activity. Similar results were found for their cytotoxicities against human cancer cell lines (A549, MCF-7, and AGS), where the highest activity was obtained with the SD-2 treatment for 72 hr at $1,000\;{\mu}g/mL$. The MCF-7 cell line was less sensitive to the powders than the other cells. From this research we found that ultrafiltration, in combination with spray-drying, is applicable for preparing protein-bound polysaccharide powders with higher antitumor activities.

• Journal of the Korea Concrete Institute
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• v.25 no.2
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• pp.217-224
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• 2013

Design provisions for the development length of headed bars in ACI 318-08 include concrete compressive strength and yield strength of headed bars as design parameters but do not consider the effects of transvers reinforcement. In addition, they have very strict limitation for clear spacing and material strengths because these provisions were developed based on limited tests. In this study, splice tests using SD600 headed bars with $2d_b$ clear spacing and transverse reinforcement were conducted. Test results show that unconfined specimens failed due to prying action and bottom cover concrete prematurely spalled. The contribution of head bearing on the anchorage strength is only 15% on average implying that unconfined specimens failed before the head bearing was not sufficiently developed. Confined specimens with stirrups placed along whole splice length have enhanced strengths in bearing as well as bond because the stirrups prevented prying action and improved bond capacity. Bond failure occurred in locally confined specimens where stirrups were placed only at the ends of splice length. The stirrups at ends of splice lengths can prevent prying action but the bond capacity did not increase. From regression analysis of test results, an equation to predict anchorage strength of headed bars was developed. The proposed equation consists of bond and bearing contributions and includes transverse reinforcement index. The average ratio of tests to predictions is 1.0 with coefficient of variation of 6%.

• Toxicological Research
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• v.13 no.1_2
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• pp.95-101
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• 1997

Ethyl carbamate (EC) is a potent teratogen in rodents and is present at low concentration in fermented foods and alcohol beverages. It has been well hypothesized that some metabolic products are responsible for the teratogenic effects of the compound. In the present study, the effects of phenobarbital (PB) on EC-induced embryotoxicity were investigated in SD rats. Six groups were constructed: EC 300 (EC 300 mg/kg/day), EC 600 (EC 600 mg/kg/day), EC 600+PB (EC 600 mg/kg/day and PB 80 mg/kg/day), PB (PB 80 mg/kg/day), DR (dietary restriction, 8 g/day/rat) and a control group. Rats of the EC 600+PB group were pretreated with phenobarbital intraperitoneally for three days to induce cytochrome P450 enzymes, followed by oral administration of EC for two consecutive days. The incidence of fetal deaths in the EC 600+PB group was higher than that of the EC 600 group(42.7 vs. 14.3%). The incidence of fetal realformations in the EC 600+PB group was higher than that of the EC 600 group (external; 7.0 vs. 4.1%, visceral; 31.4 vs. 11.3%, skeletal; 11.1 vs. 6.5%). There was no embryotoxicity in the control, EC 300, PB and DR groups. These results show that the pretreatment with phenobarbital augments EC-induced embryotoxicity in rats, indicating an evidence that metabolic activation by cytochrome P450 may be the major pathway of EC to its embryotoxic forms.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.605-608
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• 2003

The water extracts of Hovenia dulcis Thunb. showed significant hepatoptotective activity against D-galactosamine/LPS and $CCl_4-toxicity$. The extracts significantly inhibited the elevation of serum ALT, AST and LDH levels. The concentration of blood alcohol were decreased from 2hr after injected in rats with Hovenia dulcis extracts.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.4
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• pp.1-12
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• 2017

This paper concerns the structural behavior of high-strength concrete beams with compressive strength of 80 MPa subjected to flexure. Main test variables were nominal yielding strength of longitudinal rebar including normal strength rebar(SD 400) and high strength rebar(SD 600), reinforcement ratio from 0.98 to 1.58% and beam section size with $200{\times}250$, $200{\times}300mm$. The nine beams were cast and tested under flexure. The study investigated ultimate flexural strength, load-deflection relationship, crack patterns, failure patterns and ductility of the test beams. Test results indicate that when rebar ratio increased flexural strength increased and ductility decreased. In addition, the number of cracks increased and the crack width decreased as the reinforcement ratio increased. The yield strength of rebar did not affect significantly load-crack width relationship. Nonlinear analysis of test beams was performed and then test results and analytical results of ultimate load were compared. Analytical results of high-strength concrete beams overall underestimated flexural strength of test beams.

• Journal of the Korea Concrete Institute
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• v.29 no.1
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• pp.43-51
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• 2017

As per current seismic design codes, diagonally reinforced coupling beams are restricted to coupling beams having aspect ratio below 4. However, a grouped diagonally reinforcement detail makes distribution of steel bars in the beam much harder, furthermore it may result in poor construction quality. This paper describes the experimental results of concrete coupling beam reinforced with high-strength steel bars (SD500 & SD600 grades). In order to improve workability for fabricating coupling beams, a headed large diameter steel bar was used in this study. Two full-scale coupling beams were fabricated and tested with variables of reinforcement details and aspect ratio. To reflect real behavior characteristic of the beam coupling shear walls, a rigid steel frame system with linked joints was set on the reaction floor. As a test result, it was noted that cracking and yielding of reinforcement were initially progressed at the coupling beam-to-shear wall joint, and were progressed to the mid-span of the coupling beam, based on the steel strain and failure modes. It was found that the coupling beams have sufficient deformation capacity for drift ratio of shear wall corresponding to the design displacement in FEMA 450-1. In this study, the headed horizontal steel bar was also efficient for coupling beams to exhibit shear performance required by seismic design codes. For detailed design for coupling beam reinforced with high-strength steel, however, research about the effect of variable aspect ratios on the structural behavior of coupling beam is suggested.