• 정보보호학회논문지
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• 제18권4호
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• pp.27-35
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• 2008

본 논문에서는 블록 암호 SCO-1[12]에 대한 연관키 차분 공격을 소개한다. 본 논문에서 소개하는 공격은 SCO-1에 대한 첫 번째 공격이며 $2^{61}$개의 연관키 선택 암호문을 이용하여 $2^{120.59}$의 SCO-1 복호화 연산을 수행하여 SCO-1의 128-비트 비밀키를 복구한다.

• Journal of Microbiology and Biotechnology
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• 제31권5호
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• pp.756-763
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• 2021

Agarose is a linear polysaccharide composed of ᴅ-galactose and 3,6-anhydro-ʟ-galactose (AHG). It is a major component of the red algal cell wall and is gaining attention as an abundant marine biomass. However, the inability to ferment AHG is considered an obstacle in the large-scale use of agarose and could be addressed by understanding AHG catabolism in agarolytic microorganisms. Since AHG catabolism was uniquely confirmed in Vibrio sp. EJY3, a gram-negative marine bacterial species, we investigated AHG metabolism in Streptomyces coelicolor A3(2), an agarolytic gram-positive soil bacterium. Based on genomic data, the SCO3486 protein (492 amino acids) and the SCO3480 protein (361 amino acids) of S. coelicolor A3(2) showed identity with H2IFE7.1 (40% identity) encoding AHG dehydrogenase and H2IFX0.1 (42% identity) encoding 3,6-anhydro-ʟ-galactonate cycloisomerase, respectively, which are involved in the initial catabolism of AHG in Vibrio sp. EJY3. Thin layer chromatography and mass spectrometry of the bioconversion products catalyzed by recombinant SCO3486 and SCO3480 proteins, revealed that SCO3486 is an AHG dehydrogenase that oxidizes AHG to 3,6-anhydro-ʟ-galactonate, and SCO3480 is a 3,6-anhydro-ʟ-galactonate cycloisomerase that converts 3,6-anhydro-ʟ-galactonate to 2-keto-3-deoxygalactonate. SCO3486 showed maximum activity at pH 6.0 at 50℃, increased activity in the presence of iron ions, and activity against various aldehyde substrates, which is quite distinct from AHG-specific H2IFE7.1 in Vibrio sp. EJY3. Therefore, the catabolic pathway of AHG seems to be similar in most agar-degrading microorganisms, but the enzymes involved appear to be very diverse.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.480-484
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• 2010

AfsR2 is a global regulatory protein that stimulates antibiotic biosynthesis in both Streptomyces lividans and S. coelicolor. Previously, various afsR2-dependent genes including a putative abaA-like regulatory gene, SCO4677, were identified through comparative DNA microarray analysis. To further identify the putative SCO4677-dependent proteins, the comparative proteomics-driven approach was applied to the SCO4677-overexpressing strains of S. lividans and S. coelicolor along with the wild-type strains. The 2D gel electrophoresis gave approximately 277 protein spots for S. lividans and 207 protein spots for S. coelicolor, showing different protein expression patterns between the SCO4677-overexpressing strains and the wild-type strains. Further MALDI-TOF analysis revealed that only 18 proteins exhibited similar expression patterns in both S. lividans and S. coelicolor, suggesting that the SCO4677 could encode an abaA-like regulator that controls a few cross-species common proteins as well as many species-specific proteins in Streptomyces species.

• Journal of Microbiology and Biotechnology
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• 제32권9호
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• pp.1134-1145
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• 2022

SCO6993 (606 amino acids) in Streptomyces coelicolor belongs to the large ATP-binding regulators of the LuxR family regulators having one DNA-binding motif. Our previous findings predicted that SCO6993 may suppress the production of pigmented antibiotics, actinorhodin, and undecylprodigiosin, in S. coelicolor, resulting in the characterization of its properties at the molecular level. SCO6993-disruptant, S. coelicolor ΔSCO6993 produced excess pigments in R2YE plates as early as the third day of culture and showed 9.0-fold and 1.8-fold increased production of actinorhodin and undecylprodigiosin in R2YE broth, respectively, compared with that by the wild strain and S. coelicolor ΔSCO6993/SCO6993⁺. Real-time polymerase chain reaction analysis showed that the transcription of actA and actII-ORF4 in the actinorhodin biosynthetic gene cluster and that of redD and redQ in the undecylprodigiosin biosynthetic gene cluster were significantly increased by SCO6993-disruptant. Electrophoretic mobility shift assay and DNase footprinting analysis confirmed that SCO6993 protein could bind only to the promoters of pathway-specific transcriptional activator genes, actII-ORF4 and redD, and a specific palindromic sequence is essential for SCO6993 binding. Moreover, SCO6993 bound to two palindromic sequences on its promoter region. These results indicate that SCO6993 suppresses the expression of other biosynthetic genes in the cluster by repressing the transcription of actII-ORF4 and redD and consequently negatively regulating antibiotic production.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1591-1600
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• 2021

Streptomyces coelicolor is a filamentous soil bacterium producing several kinds of antibiotics. S. coelicolor abs8752 is an abs (antibiotic synthesis deficient)-type mutation at the absR locus; it is characterized by an incapacity to produce any of the four antibiotics synthesized by its parental strain J1501. A chromosomal DNA fragment from S. coelicolor J1501, capable of complementing the abs- phenotype of the abs8752 mutant, was cloned and analyzed. DNA sequencing revealed that two complete ORFs (SCO6992 and SCO6993) were present in opposite directions in the clone. Introduction of SCO6992 in the mutant strain resulted in a remarkable increase in the production of two pigmented antibiotics, actinorhodin and undecylprodigiosin, in S. coelicolor J1501 and abs8752. However, introduction of SCO6993 did not show any significant difference compared to the control, suggesting that SCO6992 is primarily involved in stimulating the biosynthesis of antibiotics in S. coelicolor. In silico analysis of SCO6992 (359 aa, 39.5 kDa) revealed that sequences homologous to SCO6992 were all annotated as hypothetical proteins. Although a metalloprotease domain with a conserved metal-binding motif was found in SCO6992, the recombinant rSCO6992 did not show any protease activity. Instead, it showed very strong β-glucuronidase activity in an API ZYM assay and toward two artificial substrates, p-nitrophenyl-β-D-glucuronide and AS-BI-β-D-glucuronide. The binding between rSCO6992 and Zn²⁺ was confirmed by circular dichroism spectroscopy. We report for the first time that SCO6992 is a novel protein with β-glucuronidase activity, that has a distinct primary structure and physiological role from those of previously reported β-glucuronidases.

• 천문학회보
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• 제32권1호
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• pp.92.2-92.2
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• 2007

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제25회 추계학술대회논문집
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• pp.307-310
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• 2005

본 연구는 프랑스의 Roxel사와 국과연간의 기술용역 계약을 통하여 추진기관 둔감화 기술과 관련된 연구를 수행한 것이다. 추진제는 연소속도가 7 MPa에서 각각 9.8 mm/s, 21.2 mm/s인 두 종류의 HTPB/AP계 혼합형 추진제를 사용하였고, 둔감 점화기와 하이브리드 연소관을 적용하였다. 2회의 Slow Cook-off(SCO) 시험과 1회의 Fast Cook-off(FCO) 시험을 수행하여 MIL STD 2105C에 규정된 반응 형태를 살펴보았다. SCO의 경우에는 두 종류의 추진제를 시용하였을 때 모두 type IV인 폭연 반응을 나타내었고, FCO의 경우에는 type V의 연소 반응을 나타내었다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 하계종합학술대회 논문집(1)
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• pp.29-32
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• 2001

This paper has simulated the PER(Packet Error Rate), ratio of received packet and payload BER of Bluetooth system with packet types of ACL(Asynchronous Connection Less) and SCO(Synchronous Connection Oriented) link over wireless Ad-hoc environment. AWGN(Additive White Gaussian Noise) and Rayleigh fading are considered as channel model, and the analysis is based on the baseband model of Bluetooth system. In terms of PER and ratio of received packet, performance of DM1 packet is almost same as those of HV1, HV2 and HV3 packets, the performances of the other packets depend on the packet types. In terms of payload BER performance, there is no difference among HV2 packet of SCO link and DM1, DM3, DM5 packets of ACL link. Moreover, there is no difference among HV3 packet of SCO link and DM1, DM3, DM5 packets of ACL link, too.

• Nutrition Research and Practice
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• 제17권2호
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• pp.192-205
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• 2023

BACKGROUND/OBJECTIVES: It is known that the renin-angiotensin system (RAS) in the brain could regulate cognitive functions as well as blood pressure. Inhibition of RAS for the improvement of cognitive function may be a new strategy, but studies so far have mostly reported on the effects of RAS inhibition by drugs, and there is no research on cognitive improvement through RAS inhibition of food ingredients. Therefore, this study investigated the effect of curcumin on blood pressure and cognitive function and its related mechanism in spontaneously hypertensive rat/Izm (SHR/Izm). MATERIALS/METHODS: Six-week-old SHR/Izm rats were divided into 5 groups: control group (CON), scopolamine group (SCO, drug for inducing cognitive deficits), positive control (SCO and tacrine [TAC]), curcumin 100 group (CUR100, SCO + Cur 100 mg/kg), and curcumin 200 group (CUR200, SCO + Cur 200 mg/kg). Changes in blood pressure, RAS, cholinergic system, and cognitive function were compared before and after cognitive impairment. RESULTS: The SCO group showed increased blood pressure and significantly reduced cognitive function based on the y-maze and passive avoidance test. Curcumin treatments significantly improved blood pressure and cognitive function compared with the SCO group. In both the CUR100 and CUR200 groups, the mRNA expressions of angiotensin-converting enzyme (ACE) and angiotensin II receptor type1 (AT1), as well as the concentrations of angiotensin II (Ang II) in brain tissue were significantly decreased. The mRNA expression of the muscarinic acetylcholine receptors (mAChRs) and acetylcholine (ACh) content was significantly increased, compared with the SCO group. CONCLUSIONS: The administration of curcumin improved blood pressure and cognitive function in SCO-induced hypertensive mice, indicating that the cholinergic system was improved by suppressing RAS and AT1 receptor expression and increasing the mAChR expression.

• 한국콘텐츠학회논문지
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• 제11권6호
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• pp.76-89
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• 2011

브랜드 로고는 상품과 서비스의 경쟁이 의미적으로 시작되는 출발점인 바, 로고 기호의 의미작용과 커뮤니케이션에 대한 분석은 브랜드 간 차이와 변별 요소의 발견에 기여할 수 있다. 이에 본 논문은 브랜드로고 기호작용에 대하여 총체적이고 일관성 있는 판독을 위한 분석 체계인 'SCoL' (Analysis frame of signification and communication for the brand logo, SCoL, 스콜) 를 개발하고, 이를 서비스의 초기 경쟁에 있어 로고 기호의 의존도가 높은 소셜 미디어 브랜드 로고 분석에 적용함으로써 제안의 타당성을 증명하고자 한다. SCoL은 브랜드 로고 기호의 4가지 형식 구성 요소를 토대로 퍼스의 도상, 지표, 상징을 통한 1단계 의미 작용 분석에 이어 2단계 분석으로 야콥슨의 이론을 적용하여 커뮤니케이션 기능을 해석할 수 있도록 고안되었다. SCoL 프레임을 적용하여 소셜 미디어 브랜드 로고 기호를 분석한 결과 소셜 미디어 브랜드들은 기호작용을 통해 고유한 의미 영역을 구축하고 있었으며, 또한 서로 다른 커뮤니케이션 기능에 의존하여 브랜드의 의미를 전달하고 있음을 발견하였다. SCoL은 분석 과정에서 브랜드 로고 기호의 구성 요소에 대한 판독 단위를 제공하여 기호학적 관점에서 로고의 의미 작용과 커뮤니케이션 기능 해석을 위한 유효한 분석 도구로 역할 하였다.