• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.423-424
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• 2014

ISO/TC 43 Acoustics 총회는 약 18개월 주기로 개최되어 음향분야 국제표준의 제,개정을 논의하고 추진한다. 최근의 ISO/TC 43회의는 2014년 5월 19일부터 23일까지 독일 베를린 DIN에서 개최되었다. ISO/TC 43의 관련 작업반(WG 1, 8, 9)회의와 함께 ISO/TC 43/SC 1 Noise 및 ISO/TC 43/SC 2 Building Acoustics의 관련 WG 회의도 진행되었다. ISO/TC 43에서는 ISO 1999 소음성 난청 평가 관련 표준을 개정하였다. ISO/TC 43/SC 1 Noise에서는 도로용 자동차의 쿨링팬 소음 측정방법, 사격장에서의 청력손실 측정방법, 헤드? 사용시 소음레벨 가이드 라인에 대한 표준 제정에 대한 NP(New Proposal)이 채택되었다. ISO/TC 43/SC 2 Building Acoustics에서는 소음 방사 효율 측정방법에 대한 표준안 제정이 제안되었다. 또한 ISO 10848-1 ~ 4 개정안 추진이 의결되었다. 건축물의 소음 평가 등급(WG 27) 및 소음성능 표시제도(WG 29)와 관련된 회의가 새롭게 진행되었다. ISO/TC 43 및 관련 SC, WG 회의를 통해 음향, 소음 전반에 걸친 국제표준의 제,개정이 의결되었으나, 국내 전문가들의 관심과 적극 참여는 부족한 것으로 판단된다. 향후 음향, 소음의 기술 및 연구 선도를 위해서는 국제표준활동에 대한 적극적인 참여와 지원이 필요하다.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1555-1563
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• 2008

A novel $\alpha$-amylase ($\alpha$-1,4-$\alpha$-D-glucan glucanohydrolase, E.C. 3.2.1.1), ScAmy43, was found in the culture medium of the phytopathogenic fungus Sclerotinia sclerotiorum grown on oats flour. Purified to homogeneity, ScAmy43 appeared as a 43 kDa monomeric enzyme, as estimated by SDS-PAGE and Superdex 75 gel filtration. The MALDI peptide mass fingerprint of ScAmy43 tryptic digest as well as internal sequence analyses indicate that the enzyme has an original primary structure when compared with other fungal a-amylases. However, the sequence of the 12 N-terminal residues is homologous with those of Aspergillus awamori and Aspergillus kawachii amylases, suggesting that the new enzyme belongs to the same GH13 glycosyl hydrolase family. Assayed with soluble starch as substrate, this enzyme displayed optimal activity at pH 4 and $55^{\circ}C$ with an apparent $K_m$ value of 1.66 mg/ml and $V_{max}$ of 0.1${\mu}mol$glucose $min^{-1}$ $ml^{-1}$. ScAmy43 activity was strongly inhibited by $Cu^{2+}$, $Mn^{2+}$, and $Ba^{2+}$, moderately by $Fe^{2+}$, and was only weakly affected by $Ca^{2+}$ addition. However, since EDTA and EGTA did not inhibit ScAmy43 activity, this enzyme is probably not a metalloprotein. DTT and $\beta$-mercaptoethanol strongly increased the enzyme activity. Starting with soluble starch as substrate, the end products were mainly maltotriose, suggesting for this enzyme an endo action.

• The Journal of the Korea Contents Association
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• v.10 no.12
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• pp.236-242
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• 2010

The porcelain fused metal was made through the progressive fused process of porcelain on substructure of metal material. The substructure was made using novel SLM method. The objective of this study was to observe the color change of porcelain using spectrophotometer equipment according to porcelain fused step and production methods after conducting the process of casting and SLM method of the substructure. The color change by step was indicated that fused opaque porcelain groups(CN1, CC1, CT1, SC1, ST1) had color difference(${\Delta}E$=30) by comparison with fused body porcelain groups(CN2, CC2, CT2, SC2, ST2) and fused glazing porcelain groups(CN3, CC3, CT3, SC3, ST3) (p<0.05). and there was no color difference between the substrates(CN, CC, CT) made by the casting method and the substrates(SC, ST) made by the SLM method. so, the color change was expressed by fused change of porcelain, and this study showed that the color of porcelain fused metal made by the SLM method can be applied clinical trials.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.04a
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• pp.423-423
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• 2014

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2012.10a
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• pp.137-137
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• 2012

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.8
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• pp.1137-1143
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• 2013

To evaluate lactation performance and changes in plasma and fecal lipopolysaccharide (LPS) concentrations in response to the supplementation of Saccharomyces cerevisiae fermentation product (SC), two dairy farms were selected. On each farm, 32 cows in early to mid lactation (21 to 140 DIM) were blocked by parity and days in milk (DIM), and randomly assigned to one of the two treatments within block (Control or 56 g SC/cow/d). Effect of SC on lactation performance (daily) and changes in blood and fecal LPS level were examined on d 0 and 28 of supplementation. The results showed that SC supplementation increased lactation performance of dairy cows on both farms. On Farm 1, milk production, 3.5% fat corrected milk (FCM), and yield of milk fat and protein were greater (p<0.01) for cows supplemented with SC. Supplementation of SC increased percentage milk fat (p = 0.029) from 81 to 110 DIM. There was no significant effect (p>0.05) of SC supplementation on percentage of milk protein, dry matter intake and feed efficiency. On Farm 2, cows supplemented with SC had a greater (p<0.05) milk yield, percentage of milk fat and milk protein, yield of milk fat and protein, 3.5% FCM and feed efficiency. Supplemental SC had no effect on LPS concentrations in feces (p>0.05) while it trended to reduce (p = 0.07 or 0.207) the concentration in plasma. The results indicate that supplemental SC can increase lactation performance of dairy cattle and has potential for reducing plasma LPS concentration.

• Journal of the Semiconductor & Display Technology
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• v.16 no.1
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• pp.22-28
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• 2017

The result of stripping process for the removal of the post etch/ash Photoresist (PR) residue on an aluminum patterned wafer by using supercritical $CO_2$ ($sc-CO_2$) mixture, was investigated by scanning of electron microscope (SEM) inspection of wafer, measuring the cloud points and visual observation of the state of $sc-CO_2$ mixtures. It was found that $sc-CO_2$ mixtures were made by mixing additives and $sc-CO_2$ should form homogeneous and transparent phase (HTP) in order to effectively and uniformly remove the post etch/ash PR residue on the aluminum patterned wafer using them. The additives were formulated by mixing and co-solvents like an amine compound and fluorosurfactants used as HTP agents, and the PR residue on the wafer were able to be rapidly and effectively removed using the $sc-CO_2$ mixture of HTP. The five kinds of additives were formulated by the recipe of mixing co-solvents and surfactants, which were able to remove PR residue on the wafer by mixing with $sc-CO_2$ at the stripping temperature range from 40 to $80^{\circ}C$. The five kinds of $sc-CO_2$ mixtures which were named as PR removers were made, which were able to form HTP within the above described stripping temperature. The cloud points of $sc-CO_2$ mixtures were measured to find correlation between them and HTP.

• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.148-156
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• 2021

Single-chain antibodies against epidermal growth factor receptor variant III (EGFRvIII) are potentially promising agents for developing antibody-based cancer treatment strategies. We described in our previous study the successful expression of an anti-EGFRvIII scFv antibody in Escherichia coli. However, we could also observe the formation of insoluble aggregates in the periplasmic space, limiting the production yield of the active product. In the present study, we investigated the mechanisms by which growth conditions could affect the expression of the soluble anti-EGFRvIII scFv antibody in small-scale E. coli NiCo21(DE3) cultures, attempting to maximize production. The secreted scFv molecules were purified using Ni-NTA magnetic beads and protein characterization was performed using SDS-PAGE and western blot analyses. We used the ImageJ software for protein quantification and determined the antigen-binding activity of the scFv antibody against the EGFRvIII protein. Our results showed that the highest percentage of soluble scFv expression could be achieved under culture conditions that combined low IPTG concentration (0.1 mM), low growth temperature (18℃), and large culture dish surface area. We found moderate-yield soluble scFv production in the culture medium after lactose-mediated induction, which was also beneficial for downstream protein processing. These findings were confirmed by conducting western blot analysis, indicating that the soluble, approximately 30-kDa scFv molecule was localized in the periplasm and the extracellular space. Moreover, the antigen-binding assay confirmed the scFv affinity against the EGFRvIII antigen. In conclusion, our study reveals that low-speed protein expression is preferable to obtain more soluble anti-EGFRvIII scFv protein in an E. coli expression system.

• Journal of Korea Society of Industrial Information Systems
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• v.26 no.6
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• pp.1-9
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• 2021

In order to increase frequency transmission efficiency, single side band(SSB) transmission systems with the complex conjugate symmetry characteristics on a frequency domain have been studied. In addition, orthogonal block codes(space-time or space-frequency block code(SFBC)) for the diversity performance gain of transmission systems have been widely researched. In this paper, we implement a 3/4-rate SFBC SSB single-carrier(SC) frequency division multiple access(FDMA) system with 4 transmit antennas. It can be shown from the simulation results that the proposed SFBC SSB SC FDMA system using the 3/4-rate 4×4 orthogonal block code outperforms the conventional SSB SC FDMA system and the 2×2 SFBC SSB SC FDMA system with 2 transmit antennas.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.387-412
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• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.