• Title/Summary/Keyword: SBF (simulated body fluid)

Search Result 45, Processing Time 0.02 seconds

Inhibition of Osteoclast differentiation based on precipitation time of titanium surfaces immersed in modified simulated body fluid (Modified simulated body fluid에 침전한 티타늄 표면에서 침전 기간에 따라 나타나는 파골 세포의 분화억제 양상)

  • Chang, Hyun-min;Heo, Seong-Joo;Kim, Seong-Kyun;Koak, Jai-Young
    • The Journal of Korean Academy of Prosthodontics
    • /
    • v.57 no.2
    • /
    • pp.142-149
    • /
    • 2019
  • Purpose: The purpose of this study is to investigate the changes of osteoclast differentiation inhibition according to the period of precipitation when titanium disks were immersed in Modified simulated body fluid (mSBF). Materials and methods: Titanium alloy (Ti grade III) disks with machined surfaces and anodized surfaces were immersed in distilled water and mSBF, respectively. The immersion periods were 7 days, 14 days, 21 days and 28 days, and the control group was immersed in distilled water for each period. RAW 264.7 cells capable of differentiating into osteoclasts were used to measure the number of adherent cells, the measurement of TRAP activity, and the expression pattern of NFATc1 by western blotting. Results: The degree of inhibition of osteoclast differentiation was found to be statistically significant when the disks were immersed in mSBF for more than 14 days on both machined surfaces and anodized surfaces. There was no correlation between immersion time and cell attachment. When the disks were immersed for more than 14 days, TRAP activity was decreased and NFATc1 expression was inhibited. Futhermore, the decrease in TRAP activity and the inhibition of NFATc1 expression remained unchanged. Conclusion: Immersion of titanium disks in mSBF for more than 14 days can prevent RAW 264.7 cells from differentiating into osteoclasts. Inhibition activity does not change even if the immersion period is for more than 14 days.

Biomimetic Apatite Precipitated on the Surface of Titanium Powder (티타늄분말의 표면에 석출된 생체모방 아파타이트)

  • Kim, Jong-Hee;Sim, Young-Uk;Yang, Tae-Young;Yoon, Seog-Young;Park, Hong-Chae
    • Journal of the Korean Ceramic Society
    • /
    • v.47 no.2
    • /
    • pp.127-131
    • /
    • 2010
  • Biomimetic whisker-like apatite was formed on thermally and NaOH-treated titanium powder in a simulated body fluid (SBF). In the early process of the SBF immersion, the surface structure of the titanium powder was loosened, possibly due to the dissolution of $Na^+$ ions on the surface of the titanium powder into SBF. When immersed for 7 days in SBF, fine precipitates appeared on the titanium surfaces; the coating layer (<200 nm in thickness) consisted of nanostructured, amorphous whisker-like and particulate phase, observed by TEM. With the extension of the immersion time to 16 days, the chrysanthemum flower type morphology of carbonated hydroxyapatite with a nanocrystallinity was developed on the surface of the titanium powder.

HREM Analysis of Apatite Formation in Modified-Simulated Body Fluid Containing Bovine Serum Albumin (소 혈청 알부민이 함유된 유사체액 내에서 아파타이트의 생성에 대한 고분해능 전자현미경 분석)

  • Kim, Woo Jeong;Lee, Kap Ho;Hong, Sun Ig
    • Korean Journal of Metals and Materials
    • /
    • v.46 no.2
    • /
    • pp.105-110
    • /
    • 2008
  • Process of the hydroxyapatite (HA) formation on bioactive titanium metal prepared by NaOH treatment in a modified-simulated body fluid (mSBF) containing bovine serum albumin (BSA) was investigated by high resolution transmission electron microscope attached with energy dispersive X-ray spectrometer (EDX). The amorphous titanate, which was formed on titanium surface by NaOH treatment, combined with the calcium ions in the liquid to form an amorphous calcium titanite. With increasing of soaking time in the liquid, an amorphous calcium titanite combined with the phosphate ions to form an amorphous calcium phosphate with low Ca/P atomic ratio, and it grows as aggregates of plate (or needle)-like substance on titanium surface. The crystalline apatite layers, which are needle-shaped with the c axis parallel to the long axis, are formed in an amorphous calcium phosphate with further increase in soaking time. The formation of needle-shaped apatite layers can be explained by electrostatic effects and difference of concentration between calcium, phosphate, and albumin ions.

Characteristics of Antibacterial Chlorhexidine-Containing Hydroxyapatite Coated on Titanium (타이타늄 상에 코팅된 클로르헥시딘 항균제를 함유한 수산화인회석의 특성)

  • Kim, Min-Hee;Hwang, Moon-Jin;Lee, Woon-Young;Park, Yeong-Joon;Song, Ho-Jun
    • Korean Journal of Dental Materials
    • /
    • v.44 no.3
    • /
    • pp.263-272
    • /
    • 2017
  • In this study, antibacterial chlorhexidine (CHX)-containing hydroxyapatite (HAp) was coated on titanium and investigated its characteristics. Ti-mSBF-CHX group was prepared by soaking titanium disks in the modified simulated body fluid (mSBF) mixed with CHX. Ti-mSBF group was coated using mSBF without CHX. Ti-mSBF-adCHX group was prepared by soaking Ti-mSBF specimen in CHX-containing solution. The crystallines clusters composed with nano-shaped crystallites were coated on the surface of the Ti-mSBF specimen. The ribbon-shaped crystallites were observed with the crystalline clusters on the Ti-mSBF-CHX specimen. The content of CHX chemical compositions was high in ribbon-shaped crystallites. HAp crystalline structure was dominant for all prepared specimens, and ${\beta}-TCP$ (tricalcium phosphate) and OCP (octacalcium phosphate) crystalline structures were observed in the Ti-mSBF-CHX specimen. FT-IR spectra showed the strong peaks of CHX in Ti-mSBF-adCHX and Ti-mSBF-CHX groups. However, after immersing in a phosphate buffered saline (PBS), CHX was rapidly released in Ti-mSBF-adCHX group, while it was slowly released in Ti-mSBF-CHX. We expect that the coating method of Ti-mSBF-CHX group could be used for protecting inflammation of titanium implant by incorporating antibacterial agent CHX into HAp layer.

HRTEM Analysis of Apatite Formed on Bioactive Titanium in Modified-SBF (수정된 유사체액 내에서 티타늄에 생성된 아파타이트의 고분해능 전자현미경에 의한 분석)

  • Kim, Hyun-Ook;Kim, Woo-Jeong;Lee, Kap-Ho;Hon, Sun-Ig
    • Korean Journal of Materials Research
    • /
    • v.17 no.8
    • /
    • pp.408-413
    • /
    • 2007
  • Process of the hydroxyapapite(HA) precipitation on bioactive titanium metal prepared by NaOH in a modified-simulated body fluid(mSBF) was investigated by high resolution transmission electron microscope (HRTEM) attached with energy dispersive X-ray spectrometer(EDX). The amorphous titanate phase on titanium surface is form by NaOH treatment and an amorphous titanate incorporated calcium and phosphate ions in the liquid to form an amorphous calcium phosphate. With increasing of soaking time in the liquid, the HA particles are observed in amorphous calcium phosphate phase with a Ca/P atomic ratio of I.30. The octacalcium phosphate (OCP) structure is not detected in HRTEM image and electron diffraction pattern. After a long soaking time, the HA particles grow as needle-like shape on titanium surface and a large particle-like aggregates of needle-like substance were observed to form on titanium surface within needle-like shape. A long axis of needle parallels to c-direction of the hexagonal HA structure.

Degradation Behavior of Hydroxyapatite with Different Crystallinity in Simulated Body Fluid Solution (의사체액에서 수산화아파타이트의 결정성에 따른 분해거동)

  • Jin, Hyeong-Ho;Kim, Dong-Hyun;Kim, Tae-Wan;Park, Hong-Chae;Yoon, Seog-Young
    • Korean Journal of Materials Research
    • /
    • v.21 no.6
    • /
    • pp.347-351
    • /
    • 2011
  • Hydroxyapatite (HAp) powders with different crystallinities were synthesized at various calcination temperatures through the co-precipitation of $Ca(OH)_2$ and $H_3PO_4$. The degradation behavior of these HAp powders with different crystallinities was assessed in a simulated body fluid solution (SBF) for 8 weeks. Below $800^{\circ}C$, the powders were nonstochiometric HAp, and the single HAp phase was successfully synthesized at $800^{\circ}C$. The degree of crystallinity of the HAp powders increased with an increasing calcination temperature and varied in a range from 39.6% to 92.5%. In the low crystallinity HAp powders, the Ca and P ion concentrations of the SBF solution increased with an increasing soaking time, which indicated that the low crystallinity HAp degraded in the SBF solution. The mass of the HAp powders linearly decreased with respect to the soaking time, and the mass loss was higher at lower crystallinities. The mass loss ranged from 0.8% to 13.2% after 8 weeks. The crystallinity of the HAp powders increased with an increasing soaking time up to 4 weeks and then decreased because of HAp degradation. The pH of the SBF solution did not change much throughout the course of these experiments. These results suggested that the crystallinity of HAp can be used to control the degradation.

Corrosion Analysis and Apatite Forming Ability of Ti and Ti-Alloys in SBF Solution (Ti과 Ti합금의 SBF에서 Apatite 형성 관찰 및 부식거동 테스트)

  • Lee Seung-Woo;Kim Yun-Jong;Choi Je-Woo;Park Joong-Keun;Kim Won-Soo;Kim Taik-Nam
    • Korean Journal of Materials Research
    • /
    • v.15 no.10
    • /
    • pp.671-677
    • /
    • 2005
  • Ti and Ti alloys are known to have excellent corrosion properties, which is an important aspect for biocompability of these implants in human body. In our study, four types of samples (Cp-Ti, Ti-6Al-4V, $0.5wt.\%$ Fe-Ti and ECAP Ti) were tested for their apatite forming ability and corrosion properties. The micropolished samples were treated with 5M NaOH solution at $60^{\circ}C$ for 24 hours. Each samples was gently washed with distilled water and heat-treated at 600"C for 1 hour. The heat-treated samples were soaked in Simulated Body Fluid (SBF) solution at $36.5^{\circ}C$ in an incubator for different period of time. The test revealed that $0.5 wt.\%$ Fe-Ti showing faster apatite growth on the surface (7th day) compared to other samples. Polarization curve test (PCT) was also carried out to determine the corrosion resistance of each samples in SBF solution. ECAP-Ti showed highest corrosion resistance compared to any other samples. $0.5wt.\%Fe-Ti$ showed higher corrosion potential and corrosion current compared to other samples.

Apatite formation on the surface treated-titanium plate in a simulated body fluid (생체유사액 침적에 따른 표면 처리된 titanium plate에 아파타이트 형성)

  • Lee, Chang-Hoon;Jin, Hyeng-Ho;Park, Hong-Chae;Yoon, Seog-Young
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.19 no.1
    • /
    • pp.19-24
    • /
    • 2009
  • The purpose of this study was to investigate the formation of apatite layer on two different titanium substrate treated with biomimetic method, Titanium plates were heat-treated at different temperatures of $400^{\circ}C$, $600^{\circ}C$, and $800^{\circ}C$ for 5 h in air atmosphere, And then, that plates were chemically treated with an alkali solution of 1 M sodium hydroxide (NaOH), The pre-treated titanium plates were soaked in the simulated body fluid (SBF) of Kokubo's recipe, After soaking for 7 days and 21 days in SBF, the coated layers formed on the titanium plates were characterized and compared with the morphology and chemical composition, The apatite formation was more activated on the titanium plates chemically treated with NaOH compared with the only heat-treated titanium plates.

Nanostructural Study of Apatite Film Biomimetically Grown in SBF (Simulated Body Fluid) (생체유사환경에서 성장된 아파타이트 층의 나노구조 연구)

  • Kim, Joung;Lee, Kap-Ho;Hong, Sun-Ig
    • Korean Journal of Materials Research
    • /
    • v.15 no.11
    • /
    • pp.690-696
    • /
    • 2005
  • The ultrastructure ore of a nanostructured apatite film nucleated from solution was studied to gain insights into that of bone minerals which is the most important constituent to sustain the strength of bones. Needle-shaped apatite crystal plates with a bimodal size distribution $(\~100\;to\;\~1000 nm)$ were randomly distributed and they were found to grow parallel to the c-axis ([002]), driven by the reduction of surface energy. Between these randomly distributed needle-shaped apatite crystals which are parallel to the film, apatite crystals (20-40nm) with the normal of the grains quasi-perpendicular to the c-axis were observed. These observations suggest that the apatite film is the interwoven structure of apatite crystals with the c-axis parallel and quasi-perpendicular to the fan. In some regions, amorphous calcium phosphate, which is a precursor of apatite, was also observed. In the amorphous phase, small crystalline particle with the size of 2-3 nm were observed. These particles were quite similar, in size and shape, to those observed in the femoral trabecular bone, suggesting the nucleation of apatites by a biomimetic process in vitro is similar to that in vivo.

Electrochemical Characteristics of Dental Implant in the Various Simulated Body Fluid and Artificial Saliva (다양한 유사체액과 인공타액에서 치과용 임플란트의 전기화학적 특성)

  • Kim, T.H.;Park, G.H.;Son, M.K.;Kim, W.G.;Jang, S.H.;Choe, H.C.
    • Journal of the Korean institute of surface engineering
    • /
    • v.41 no.5
    • /
    • pp.226-231
    • /
    • 2008
  • Titanium and its alloy have been widely used in dental implant and orthopedic prostheses. Electrochemical characteristics of dental implant in the various simulated body fluids have been researched by using electrochemical methods. Ti-6Al-4V alloy implant was used for corrosion test in 0.9% NaCl, artificial saliva and simulated body fluids. The surface morphology was observed using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDX). The electrochemical stability was investigated using potentiosat (EG&G Co, 263A). The corrosion surface was observed using scanning electron microscopy (SEM). From the results of potentiodynamic test in various solution, the current density of implant tested in SBF and AS solution was lower than that of implant tested in 0.9% NaCl solution. From the results of passive film stability test, the variation of current density at constant 250 mV showed the consistent with time in the case of implant tested in SBF and AS solution, whereas, the current density at constant 250mV in the case of implant tested in 0.9% NaCl solution showed higher compared to SBF and AS solution as time increased. From the results of cyclic potentiodynamic test, the pitting potential and |$E_{pit}\;-\;E_{corr}$| of implant tested in SBF and AS solution were higher than those of implant tested in 0.9% NaCl solution.