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HREM Analysis of Apatite Formation in Modified-Simulated Body Fluid Containing Bovine Serum Albumin  

Kim, Woo Jeong (Division of Nano Technology, Chungnam National University)
Lee, Kap Ho (Division of Nano Technology, Chungnam National University)
Hong, Sun Ig (Division of Nano Technology, Chungnam National University)
Publication Information
Korean Journal of Metals and Materials / v.46, no.2, 2008 , pp. 105-110 More about this Journal
Abstract
Process of the hydroxyapatite (HA) formation on bioactive titanium metal prepared by NaOH treatment in a modified-simulated body fluid (mSBF) containing bovine serum albumin (BSA) was investigated by high resolution transmission electron microscope attached with energy dispersive X-ray spectrometer (EDX). The amorphous titanate, which was formed on titanium surface by NaOH treatment, combined with the calcium ions in the liquid to form an amorphous calcium titanite. With increasing of soaking time in the liquid, an amorphous calcium titanite combined with the phosphate ions to form an amorphous calcium phosphate with low Ca/P atomic ratio, and it grows as aggregates of plate (or needle)-like substance on titanium surface. The crystalline apatite layers, which are needle-shaped with the c axis parallel to the long axis, are formed in an amorphous calcium phosphate with further increase in soaking time. The formation of needle-shaped apatite layers can be explained by electrostatic effects and difference of concentration between calcium, phosphate, and albumin ions.
Keywords
hydroxyapatite(HA); modified-simulated body fluid(mSBF); bovine serum albumin(BSA); high resolution electron microscopy(HREM);
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