• Title/Summary/Keyword: SAG3

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Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis

  • Khanaliha, Khadijeh;Motazedian, Mohammad Hossein;Kazemi, Bahram;Shahriari, Bahador;Bandehpour, Mojgan;Sharifniya, Zarin
    • Parasites, Hosts and Diseases
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    • v.52 no.2
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    • pp.137-142
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    • 2014
  • Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.

The Space Vector Detection based Three-Phase Hybrid Series Active Power Filter for Compensating Dynamic Voltage Sag and Harmonic Current (순시전압 sag 및 고조파 전류 보상을 위한 공간벡터 검출법 기반의 3상 하이브리드 직렬형 능동전력필터)

  • 양승환;정영국;임영철
    • The Transactions of the Korean Institute of Power Electronics
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    • v.9 no.4
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    • pp.303-310
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    • 2004
  • In this paper, for compensating dynamic voltage sag and harmonic current, 3-phase hybrid series active power filter based on the space vector detection is proposed. The Space vector algorithm for detecting the voltage sag and the harmonic current in compared with conventional theory is a simple method for calculating the compensating reference without any coordinated transformation. The effectiveness of the proposed system is verified by the PSIM simulation in the steady state and the transient state, which the proposed system is able to simultaneously compensate harmonics and source voltage unbalance / sag.

Development of Three Phase 10kW Voltage Sag Compensator (SEMI F47을 만족하는 10kW급 3상 전압 새그 보상기 개발)

  • Chae, Seung-Woo;Cho, Hyun-Sik;Lee, Il-Yong;Kong, Se-Il;Han, Byung-Moon;Cha, Han-Ju
    • The Transactions of the Korean Institute of Power Electronics
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    • v.17 no.3
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    • pp.198-204
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    • 2012
  • 3-Phase voltage sag compensator protects a critical load from grid sags. The paper presents an algorithm and design of 3-phase voltage compensator. Compensator algorithm consists of a 3-phase voltage sag detection, thyrister commutation method and inverter output voltage control. The compensator satisfies SEMI F47 standard and 10kW 3-phase voltage sag compensator prototype is assembled. Validity of the proposed compensator is verified by simulation and experiment.

Three Phase Voltage Sag Compensator Using Three Phase AC/AC Chopper (3상 AC/AC 초퍼를 이용한 3상 Voltage Sag Compensator)

  • Kim, Dong-Kyu;Choi, Nam-Sup
    • Proceedings of the KIPE Conference
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    • 2014.07a
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    • pp.144-145
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    • 2014
  • 본 논문에서는 Buck type의 3상 AC/AC 초퍼를 이용한 3상 voltage sag compensator를 제안한다. 제안된 회로는 sag보상 동작 시 사용된 변압기의 누설리액턴스가 3상 PWM Buck AC/AC converter에 미치는 영향이 작아 동작효율이 높고, sag가 발생하지 않은 상태일 때 입력과 출력이 절연되는 장점이 있다. 본 논문에서는 제안된 회로의 동작 및 특성을 설명하고, 타당성을 입증하기 위해 PSIM으로 시뮬레이션 하였다.

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Voltage Sag Detection Algorithm for Instantaneous Voltage Sag Corrector

  • Lee, Sang-Hoon;Choi, Jae-Ho
    • Journal of Power Electronics
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    • v.2 no.3
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    • pp.162-170
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    • 2002
  • Voltage sag detection algorithm for voltage sag corrector is proposed in this paper. To quantify the standard of voltage unbalance under the faulted conditions, the 3-phase unbalanced voltages are decomposed into two balanced 3-phase symmetrical components of the positive and negative sequence voltages, which is defined by the magnitude factor (MF) and unbalance factor (UF). It is analyzed that MWF and UF values are given as the dc constant values even though under the voltage unbalance condition. This paper also proposes the control scheme of the instantaneous voltage sag corrector based on this detection algorithm. The validity of the proposed algorithm is verified through the EMTDC simulation and experiments.

Sag & Swell Detection by Phase Voltage Compensation in 3-Phase Unbalanced Grid (3상 불평형 계통에서의 상 전압 보상을 통한 Sag 및 Swell 검출)

  • Kim, Min-Gi;Kim, Jun-Gu;Jung, Yong-Chae;Won, Chun-Yuen
    • Proceedings of the KIPE Conference
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    • 2013.11a
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    • pp.258-259
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    • 2013
  • Load connection or disconnection makes instantaneous sag & swell in 3-phase grid. When unbalance state occurs, between sensed phase voltage and actual phase voltage may have discrepancy. It makes difficult to detect accurate sag & swell, so it is hard to satisfy the standard for switching ESS system to UPS mode. In this paper, we analyzed unbalanced 3-phase voltage, and compensated the actual sag & swell magnitude.

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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

  • Chong, Chom-Kyu;Jeong, Woo-Seog;Kim, Hak-Yong;An, Dong-Jun;Jeoung, Hye-Young;Ryu, Jeong-Eun;Ko, A-Ra;Kim, Yong-Joo;Hong, Sung-Jong;Yang, Zhaoshou;Nam, Ho-Woo
    • Parasites, Hosts and Diseases
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    • v.49 no.3
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    • pp.207-212
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    • 2011
  • Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

A Dynamic Characteristics on Non-linear Voltage Electric Equipment under Voltage Sag (순간전압강하에 대한 비선형 저압전기기기의 동적특성)

  • Lee, Hyun-Chul;Jeong, Sung-Won;Gim, Jae-Hyeon;Lee, Geun-Joon
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.3
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    • pp.336-343
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    • 2008
  • In this study, a transition of power quality was characterized by non-linear voltage electric equipments under voltage sag. The test was inputted voltage sag to IPC(Sag Generator) from AVR, and then to equipments by IPC which adjust voltage magnitude and duration. The load test which non-linear voltage electric equipments used PLC, Magnetic Contactor, SMPS, HID Lamp. The test result was different from each other according to a manufacturing companys, models, and equipments. PLC was greatly described to be stabilized voltage sag in case of no load then rated load. Magnetic Contactor was made a difference to phase angles on voltage sag, which was $0^{\circ}$, $30^{\circ}$, $60^{\circ}$, $90^{\circ}$. HPD Lamp was described to be stabilized the sodium lamp, and to be unstabilized the metal hailed lamp. The test result was showed CBEMA curve that stand for evaluated responsiveness of voltage sag. This study was tested description to dynamic characteristics on non-linear voltage electric equipment under voltage sag. There was hoped that power system designed the essential particulars.

Analysis of the Contact Wire Wear Pattern According to the Pre-sag (사전이도에 따른 전차선 마모 패턴 분석)

  • Lee, In-Hee;Park, Jae-Young
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.64 no.10
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    • pp.1501-1507
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    • 2015
  • The contact wire pre-sag in railway overhead catenary has been considered as a innovative idea which improves the current collection quality and is expected to evenly distribute wear along a span. However, long term studies on the effects of pre-sag, especially on the quantitative wear pattern of the contact wire, have not yet been reported. Therefore, in this study, the pre-sag and wear pattern of the contact wire along a span from long term operation of railway in Korea was surveyed. The examination was conducted on 3 lines, the Gyeongbu high-speed line with pre-sag of span/2000, the Gyeongbu existing line without pre-sag, and Gyeongchun line with pre-sag installation on 1/1000 of its span. The wear measurements of 58 sample spans were examined. The wear pattern analysis show no interrelationships between pre-sag and evenly distributed wear, but more wear occurred at the center of a span compared to the ends of the span, especially more noticeable as span length increases.

Simulation and Testing of the Effect of Current Collection Performance According to Pre-sag in 400km/h Overhead Contact Lines (400km/h 전차선로에서 사전이도가 집전성능에 미치는 영향에 대한 시뮬레이션 및 시험)

  • Kwon, Sam Young;Cho, Yong Hyeon;Lee, Kiwon;Oh, Hyuck Keun
    • Journal of the Korean Society for Railway
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    • v.19 no.3
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    • pp.288-296
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    • 2016
  • A 400km/h simple catenary system was constructed as a test line in Korea. In the design stage of this system, the pre-sag was one of the engineering issues most focused on. It is known that the pre-sag improves the current collection performance in a certain band of high speed. However, the effect of pre-sag at 400km/h has not yet been established. To grasp a better pre-sag in the 400km/h catenary, we transacted the dynamic performance prediction simulation between catenary and pantograph under conditions of 0 and 1/3000 pre-sag. The level of 0 pre-sag was adapted for the 400km/h catenary design after reviewing predictions. We constituted the 1/3000 pre-sag sample section (about 1km) while constructing the 400km/h catenary test-bed (28km) of 0 pre-sag. With a HEMU-430X train, the contact forces were measured in the test-bed including the pre-sag sample section. In this paper, the predicted and measured dynamic performance values (contact forces) for 0 and 1/3000 pre-sag are described and compared. It is conclusively confirmed by analytical and experimental examination that the non pre-sag showed better dynamic (current collection) performance than that of the 1/3000 pre-sag for the 400km/h catenary system.