Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Recombinant SAG1, SAG2, and SAG3 Antigens for Serodiagnosis of Toxoplasmosis

  • Khanaliha, Khadijeh (Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences) ;
  • Motazedian, Mohammad Hossein (Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences) ;
  • Kazemi, Bahram (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Shahriari, Bahador (Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences) ;
  • Bandehpour, Mojgan (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Sharifniya, Zarin (Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences)
  • Received : 2013.07.31
  • Accepted : 2014.01.25
  • Published : 2014.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.

Keywords

References

  1. Parmley SF, Sgarlato GD, Mark J, Prince JB, Remington JS. Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii. J Clin Microbiol 1992; 30: 1127-1133.
  2. Remington JS, McLeod R, Desmonts G. Toxoplasmosis. In Remington JS, Klein JO, eds,. Infectious diseases of the fetus and newborn infant. Philadelphia, Pennsylvania, USA. Saunders WB. 1995, p 140-267.
  3. Wong SY, Remington JS. Toxoplasma in pregnancy. Clin Infect Dis 1994; 18: 727-729.
  4. Handman E, Remington JS. Antibody responses to Toxoplasma antigens in mice infected with strains of different virulence. Infect Immun 1980; 29: 215-220.
  5. Buffolano W, Beghetto E, Del Pezzo M, Spadoni A, Di Cristina M, Petersen E, Gargano N. Use of recombinant antigens for early postnatal diagnosis of congenital toxoplasmosis. J Clin Microbiol 2005; 43: 5916-5924. https://doi.org/10.1128/JCM.43.12.5916-5924.2005
  6. Kasper LH, Bradley MS, Pfefferkorn ER. Identification of stage specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol 1984; 132: 443-449.
  7. Grimwood J, Smith JE. Toxoplasma gondii: the role of parasite surface and secreted proteins in host cell invasion. Int J Parasitol 1996; 26: 169-173. https://doi.org/10.1016/0020-7519(95)00103-4
  8. Aubert D, Maine GT, Villena I, Hunt JC, Howard L, Sheu M, Brojanac S, Chovan LE, Nowlan SF, Pinon JM. Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J Clin Microbiol 2000; 38: 1144-1150.
  9. Kasper LH. Identification of stage-specific antigens of Toxoplasma gondii. Infect Immun 1989; 57: 668-672.
  10. Huang X, Xuan X, Kimbita EN, Battur B, Miyazawa T, Fukumoto S, Mishima M, Makala LH, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Mikami T, Igarashi I. Development and evaluation of an enzyme linked immunosorbent assay with recombinant SAG2 for the diagnosis of Toxoplasma gondii infection in cats. J Parasitol 2002; 88: 804-807. https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2
  11. Kimbita EN, Xuan X, Huang X, Miyazawa T, Fukumoto S, Mishima M, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Suzuki N, Mikami T, Igarashi I. Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1. Vet Parasitol 2001; 102: 35-44. https://doi.org/10.1016/S0304-4017(01)00522-2
  12. Pietkiewicz H, Hiszczyriska-Sawicka E, Kur J, Petersen E, Nielsen HV, Stankiewicz M, Andrzejewska I, Myjak P. Usefulness of Toxoplasma gondii-specific recombinant antigens in serodiagnosis of human toxoplasmosis. J Clin Microbiol 2004; 42: 1779-1781. https://doi.org/10.1128/JCM.42.4.1779-1781.2004
  13. Burg JL, Perelman D, Kasper LH, Ware PL, Boothroyd JC. Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol 1988; 141: 3584-3591.
  14. Prince JB, Auer KL, Huskinson J, Parmley SF, Araujo FG, Remington JS. Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii. Mol Biochem Parasitol 1990; 43: 97-106. https://doi.org/10.1016/0166-6851(90)90134-8
  15. Jacquet A, Coulon L, De Nève J, Daminet V, Haumont M, Garcia L, Bollen A, Jurado M, Biemans R. The surface antigen SAG3 mediates the attachment of Toxoplasma gondii to cell-surface proteoglycans. Mol Biochem Parasitol 2001; 116: 35-44. https://doi.org/10.1016/S0166-6851(01)00297-3
  16. Lee YH, Shin DW, Lee JH, Nam HW, Ahn MH. Vaccination against murine toxoplasmosis using recombinant Toxoplasma gondii SAG3 antigen alone or in combination with Quil A. Yonsei Med J 2007; 48: 396-404. https://doi.org/10.3349/ymj.2007.48.3.396
  17. Beaman MH, McCabe RE, Wong SY, Remington JS. Toxoplasma gondii. In Mandel GL, Bennett JE, Dolin R eds, Principles and Practices of Infectious Diseases, 4th ed. New York, New York, USA. Churchill and Livingstone, Inc. 1995, p 2455-2475.
  18. Johnson AM, Illana S .Cloning of T. gondii gene fragments encoding diagnostic antigens. Gene 1991; 99: 127-132. https://doi.org/10.1016/0378-1119(91)90044-C
  19. Tenter AM, Johnson AM. Human antibody response to the nucleoside triphosphate hydrolase of T. gondii. J Immunoassay 1990; 11: 579-590. https://doi.org/10.1080/01971529008055051
  20. Tenter AM, Johnson AM. Recognition of recombinant T. gondii antigens by human sera in an ELISA. Parasitol Res 1991; 77: 197-203. https://doi.org/10.1007/BF00930858
  21. Kazemi B, Bandehpour M, Maghen L, Solgi GH. Gene cloning of 30 kDa Toxoplasma gondii tachyzoites surface antigen (SAG). Iranian J Parasitol 2007; 2: 1-8.
  22. Arabpour M, Bandehpour M, Niyyati M, Abdollahi SH, Kochaki A, Kazemi B. Cloning and expression of Toxoplasma gondii tachyzoite P22 protein. Afr J Biotechnol 2011; 10: 7746-7750.
  23. Kazemi B, Maghen L, Bandehpour M, Shahabi S, Haghighi A. Gene cloning of p43 surface protein of Toxoplasma gondii tachyzoite and bradyzoite (SAG3). GTMB 2007; 11: 113-116.
  24. Hanahan D. Studies on transformation on E. coli with plasmids. J Mol Biol 1983; 166: 557-580. https://doi.org/10.1016/S0022-2836(83)80284-8
  25. Jalallou N, Bandehpour M, Khazan M, Haghighi A, Abdollahi SH, Kazemi B. Recombinant SAG1 antigen to detect Toxoplasma gondii specific immunoglobulin G in human sera by ELISA test. Iranian J Parasitol 2010; 5: 1-9.
  26. Khanaliha K, Motazedian MH, Sarkari B, Bandehpour M, Sharifnia Z, Kazemi B. Expression and purification of P43 Toxoplasma gondii surface antigen. Iranian J Parasitol 2012; 7: 48-52.
  27. Beghetto E, Buffolano W, Spadoni A, Del Pezzo M, Cristina MD, Minenkova O, Petersen E, Felici F, Gargano N. Use of an immunoglobulin G avidity assay based on recombinant antigens for diagnosis of primary Toxoplasma gondii infection infection during pregnancy. J Clin Microbiol 2003; 41: 5414-5418. https://doi.org/10.1128/JCM.41.12.5414-5418.2003
  28. Selseleh MM, Keshavarz H, Mohebali M, Shojaee S, Modarressi M, Eshragian M. Production and evaluation of Toxoplasma gondii recombinant surface antigen 1 (SAG1) for serodiagnosis of acute and chronic Toxoplasma infection in human sera. Iranian J Parasitol 2012; 7: 1-9.
  29. Gatkowska J, Hiszczynska-Sawicka E, Kur J, Holec L, Dlugonska H. Toxoplasma gondii: an evaluation of diagnostic value of recombinant antigens in a murine model. Exp Parasitol 2006; 114: 220-227. https://doi.org/10.1016/j.exppara.2006.03.011
  30. Ling Lau Y, Yik Fong M. Toxoplasma gondii: serological characterization and immunogenicity of recombinant surface antigen 2 (SAG2) expressed in the yeast Pichia pastoris. Exp Parasitol 2008; 119: 373-378. https://doi.org/10.1016/j.exppara.2008.03.016
  31. Kotresha D, Noordin R. Recombinant proteins in the diagnosis of toxoplasmosis. APMIS 2010; 118: 529-542.
  32. Dai J, Jiang M, Wang Y, Qu L, Gong R, Si J. Evaluation of a recombinant multiepitope peptide for serodiagnosis of Toxoplasma gondii infection. Clin Vaccine Immunol 2012; 19: 338-342. https://doi.org/10.1128/CVI.05553-11
  33. Dai JF, Jiang M, Qu LL, Sun L, Wang YY, Gong LL, Gong RJ, Si J. Toxoplasma gondii: enzyme-linked immunosorbent assay based on a recombinant multiepitope peptide for distinguishing recent from past infection in human sera. Exp Parasitol 2013; 133: 95-100. https://doi.org/10.1016/j.exppara.2012.10.016

Cited by

  1. Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells vol.8, pp.3, 2015, https://doi.org/10.5812/jjm.22570
  2. Cloning and Sequencing of Truncated Toxoplasma gondii Subtilisin-Like 1 Antigen vol.18, pp.8, 2014, https://doi.org/10.17795/zjrms-7938
  3. Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay vol.12, pp.1, 2014, https://doi.org/10.5812/archcid.35612
  4. P35 and P22 Toxoplasma gondii antigens abbreviate regions to diagnose acquired toxoplasmosis during pregnancy: toward single-sample assays vol.55, pp.4, 2017, https://doi.org/10.1515/cclm-2016-0331
  5. P35 and P22 Toxoplasma gondii antigens abbreviate regions to diagnose acquired toxoplasmosis during pregnancy: toward single-sample assays vol.55, pp.4, 2017, https://doi.org/10.1515/cclm-2016-0331
  6. Antigens to detect the acute phase of toxoplasmosis in pregnant women: standardized comparison vol.12, pp.5, 2018, https://doi.org/10.2217/bmm-2017-0345
  7. Research Progress of Antigens Related to the Process of Toxoplasma gondii Invading Host Cell vol.7, pp.6, 2014, https://doi.org/10.12677/ojns.2019.76068
  8. Evaluation of a PCR assay for diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell among HIV/AIDS patients vol.44, pp.1, 2014, https://doi.org/10.1007/s12639-019-01176-6
  9. Toxoplasma gondii seropositivity and serointensity and cognitive function in adults vol.14, pp.10, 2020, https://doi.org/10.1371/journal.pntd.0008733
  10. Human toxoplasmosis in Mozambique: gaps in knowledge and research opportunities vol.13, pp.1, 2020, https://doi.org/10.1186/s13071-020-04441-3
  11. Seroprevalence and Epidemiology of Toxoplasma gondii in Animals in the Qinghai-Tibetan Plateau Area, China vol.10, pp.4, 2014, https://doi.org/10.3390/pathogens10040432
  12. Detection of Toxoplasma gondii Infections using Virus-Like Particles Displaying T. gondii ROP4 Antigen vol.59, pp.6, 2014, https://doi.org/10.3347/kjp.2021.59.6.565