• Journal of Life Science
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• v.26 no.8
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• pp.895-903
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• 2016

This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence- associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.21 no.10
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• pp.954-960
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• 2008

The effects of elastic modulus coefficient and linear expansion coefficient of overhead distribution power line(ACSR $58 mm^2$) on sag behavior in distribution line have been investigated to clarify the difference between specification and experimental level. The elastic modulus coefficients of Al wire and steel wire were $5,182.6 kgf/mm^2,\;18,348.8 kgf/mm^2$, respectively Therefore, the computational composition elastic modulus coefficient of the power line was $7,063.5 kgf/mm^2$, while that of experimentally measured was $7681.1 kgf/mm^2$. As a result, we found that elastic modulus coefficient which was experimentally measured was higher than that of computational by 8.7 %. However, when planner designs the sag of disoibution line, the elastic modulus coefficient of power line $8,400 kgf/mm^2$ should be generally adopted. These two different using values lead to the sag difference of 0.62 m. The other results will be discussed.

• Journal of Power Electronics
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• v.13 no.2
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• pp.304-312
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• 2013

The most common power quality problems in distribution systems are related to unbalanced voltage sags. Voltage sags must be detected quickly and corrected in a minimum amount of time. One of the most widely used methods for sag detection is based on the d-q transformation. This method has the disadvantage of missing the detection of unbalanced faults, because this method uses a voltage sag level signal obtained from the average of 3 phases for sag detection. In this paper, an adaptive filter sag detection method is proposed for Dynamic Voltage Restorers (DVR) under unbalanced fault conditions. The proposed DVR controller is able to detect balanced, unbalanced and single phase voltage sags. A novel reference voltage generation method is also presented. To validate the proposed control methods, a 3-phase DSP controlling a DVR prototype with a power rating of 1.5-kVA has been developed. Finally, experimental results are presented to verify the performance of the proposed control methods.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.63 no.2
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• pp.230-236
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• 2014

Superconducting fault current limiter (SFCL) has been expected as one of more effective solutions for decreasing fault current instantaneously and various types of SFCLs have been developed to apply into real power system. Recently, the application of the SFCL in a power distribution system has been reported to be contributed to the suppression of the voltage sag as well as the limitation of the fault current. However, the suppressing effect of voltage sag by the SFCL depends on component of its impedance and its application location in a power distribution system considering the recloser-recloser coordination. This paper analyzed the voltage sag caused by recloser-recloser coordination in a power distribution system and the suppression of the voltage sag due to the application location of the SFCL in a power distribution system was discussed through the PSCAD/EMTDC simulation.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.2
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• pp.159-163
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• 2010

The superconducting fault current limiter (SFCL) plays a role in compensating the voltage sag of the sound feeder adjacent to the fault feeder as well as the fault current limiting operation of the fault feeder. Especially, the SFCL using magnetic coupling of two coils with parallel connection has different voltage sag compensating and current limiting characteristics due to the winding direction and the inductance ratio of two coils. In this paper, the current limiting and the voltage sag compensating characteristics of a SFCL using magnetic coupling of parallel connected two coils were analyzed. Through the analysis on the experimental results considering the winding direction of two coils, the SFCL designed with the additive polarity winding was shown to have the higher limited fault current than the SFCL designed with the subtractive polarity winding. In addition, it could be confirmed that the higher fault current limitation of the SFCL could be contributed to the higher load voltage sag compensation.

• Investigative Magnetic Resonance Imaging
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• v.17 no.2
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• pp.91-100
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• 2013

Purpose : To evaluate the usefulness of cervicothoracic spine sagittal T2-weighted images (CT SAG T2WIs) included in routine lumbar spine MRI. Materials and Methods: Institutional review board approval was obtained and informed consents were waived for this retrospective study. The study group comprised 2,113 patients who underwent lumbar spine MRI from January 2005 to December 2005. CT SAG T2WIs were added in the routine lumbar spine MRIs. Radiologic reports were reviewed retrospectively for pathologic lesions on CT SAG T2WIs by one radiologist. Information of additional cervical or thoracic spine MRI and/or CT for further evaluation of positive findings on CT SAG T2WIs and their treatment were collected by retrospectively reviewing medical records. Results: The CT SAG T2WIs revealed 142 pathologic lesions in 139 (6.58%) of the 2,113 patients. They were easily obtained without positional change in a scan time of less than 2 minutes. Additional cervical or thoracic spine MRI and/or CT for positive findings on CT SAG T2WIs were performed in 13 patients. Seven patients underwent surgical treatment. Conclusion: CT SAG T2WIs included in routine lumbar spine MRI were useful in finding the pathologic lesions in cervicothoracic spine for the patients who assumed to have lesions in lumbar spine.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.687-694
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• 1995

Alkalophilic Bacillus sp. HJ-12 producing $\beta $-1, 4-D-arabinogalactanase was isolated from soil in the alkalic condition, pH 10.0. $\beta $-1, 4-D-arabinogalactanase was maximaly produced in the medium consisting of 2% soybean arabinogalactan (SAG), 0.5% yeast extract, 0.5% polypeptone, 0.5% NaCl, 0.1% K$_{2}$HPO$_{4}$, 0.02% MgSO$_{4}$$\cdot $7H$_{2}$O, 0.1% Na$_{2}$CO$_{3}$ under the aerobic condition (pH 8.2). $\beta $-1, 4-D-arabinogalactanase is inducible enzyme so that its activity has been increased 10 fold in the SAG medium than in the glucose medium. Through the ammonium sulfate precipitation, DEAE- Sephadex A-50 ion chromatography, and Sephadex G-75 gel chromatography procedures, this enzyme was purified with a single protein of 11% vield and 110 fold's purity. $\beta $-1, 4-D-arabinogalactanase is endo type enzyme producing ollgosaccharide from SAG.

• Parasites, Hosts and Diseases
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• v.49 no.3
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• pp.207-212
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• 2011

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.503-510
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• 2013

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.

• Molecules and Cells
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• v.42 no.9
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• pp.661-671
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• 2019

Adipose tissue-derived mesenchymal stem cells (ADSCs) are promising for regenerating degenerated intervertebral discs (IVDs), but the low efficiency of nucleus pulposus (NP)-specific differentiation limits their clinical applications. The Sonic hedgehog (Shh) signaling pathway is important in NP-specific differentiation of ADSCs, and Smoothened Agonist (SAG) is a highly specific and effective agonist of Shh signaling. In this study, we proposed a new differentiation strategy with the use of the small molecule SAG. The NP-specific differentiation and extracellular matrix (ECM) synthesis of ADSCs were measured in vitro, and the regenerative effects of SAG pretreated ADSCs in degenerated IVDs were verified in vivo. The results showed that the combination of SAG and transforming growth factor-${\beta}3$ ($TGF-{\beta}3$) is able to increase the ECM synthesis of ADSCs. In addition, the gene and protein expression levels of NP-specific markers were increased by treatment with SAG and $TGF-{\beta}3$. Furthermore, SAG pretreated ADSCs can also improve the disc height, water content, ECM content, and structure of degenerated IVDs in vivo. Our new differentiation scheme has high efficiency in inducing NP-specific differentiation of ADSCs and is promising for stem cell-based treatment of degenerated IVDs.