• Parasites, Hosts and Diseases
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• v.52 no.2
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• pp.137-142
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• 2014

Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii.

• Proceedings of the Korean Society of Computer Information Conference
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• 2011.01a
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• pp.81-84
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• 2011

본 논문은 전압 이벤트 현상 중 순간전압강하(Sag) 현상에 초점을 맞추었다. Sag 현상의 심각한 정도를 표현하는 심각도(Voltage Sag Severity) 지수는 동일 지속시간에 대한 임계치와의 비로 표현하였다. 제안하는 확장된 심각도(Expanded Severity) 지수는 sag현상의 분포에 따른 일시반복성의 정보를 표현하였다. 기존의 임계치를 표현하는 ITIC curve를 기반으로 된 심각도와 sag 현상이 발생하는 지속시간-전압 그래프의 분포를 fuzzy clustering을 통하여 medoid를 측정하고, medoid의 심각도와 실제 임계치에 근접한 sag 지점의 심각도를 계산하여 비교하였다. 확장된 심각도 지수는 심각도가 높은 현상들과의 연계성을 나타내는 지수로 심각한 정도의 수치 정보 이외에 일시적인 현상인지 지속 반복적인 현상인지를 0과 1사이의 수치로 표현하였고, 실험을 통하여 입증하였다.

• Journal of the Korean Society for Railway
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• v.19 no.3
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• pp.288-296
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• 2016

A 400km/h simple catenary system was constructed as a test line in Korea. In the design stage of this system, the pre-sag was one of the engineering issues most focused on. It is known that the pre-sag improves the current collection performance in a certain band of high speed. However, the effect of pre-sag at 400km/h has not yet been established. To grasp a better pre-sag in the 400km/h catenary, we transacted the dynamic performance prediction simulation between catenary and pantograph under conditions of 0 and 1/3000 pre-sag. The level of 0 pre-sag was adapted for the 400km/h catenary design after reviewing predictions. We constituted the 1/3000 pre-sag sample section (about 1km) while constructing the 400km/h catenary test-bed (28km) of 0 pre-sag. With a HEMU-430X train, the contact forces were measured in the test-bed including the pre-sag sample section. In this paper, the predicted and measured dynamic performance values (contact forces) for 0 and 1/3000 pre-sag are described and compared. It is conclusively confirmed by analytical and experimental examination that the non pre-sag showed better dynamic (current collection) performance than that of the 1/3000 pre-sag for the 400km/h catenary system.

• Ocean Systems Engineering
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• v.10 no.1
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• pp.67-86
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• 2020

The main goal of this study is to investigate the free vibration analysis of a large sag catenary with application to the jumper in hybrid riser system. The equation of motion is derived by using the variational method based on the virtual work principle. The finite element method is applied to evaluate the numerical solutions. The large sag catenary is utilized as an initial configuration for vibration analysis. The nonlinearity due to the large sag curvature of static configuration is taken into account in the element stiffness matrix. The natural frequencies of large sag catenary and their corresponding mode shapes are determined by solving the eigenvalue problem. The numerical examples of a large sag catenary jumpers are presented. The influences of bending rigidity and large sag shape on the free vibration behaviors of the catenary jumper are provided. The results indicate that the increase in sag reduces the jumper natural frequencies. The corresponding mode shapes of the jumper with large sag catenary shape are comprised of normal and tangential displacements. The large sag curvature including in the element stiffness matrix increases the natural frequency especially for a case of very large sag shape. Mostly, the mode shapes of jumper are dominated by the normal displacement, however, the tangential displacement significantly occurs around the lowest point of sag. The increase in degree of inclination of the catenary tends to increase the natural frequencies.

• Parasites, Hosts and Diseases
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• v.49 no.3
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• pp.207-212
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• 2011

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

• Archives of Pharmacal Research
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• v.28 no.7
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• pp.848-853
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• 2005

Synurus deltoides was previously found to possess significant anti-inflammatory activity especially against chronic inflammation, and strong analgesic activity in vivo. In this study, new anti-inflammatory formulation containing S. deltoides extract as a major ingredient was prepared and in vivo activity was evaluated. The plausible action mechanism was also investigated. The new formulation (SAG) contains 1 part of S. deltoides extract, 0.9 part of Angelica gigas extract and 0.9 part of glucosamine sulfate (w/w). SAG inhibited dose-dependently edematic response of arachidonic acid (AA)- and 12-O-tetradecanoyl 13-acetate (TPA)-induced ear edema in mice, which is an animal model of acute inflammation. SAG showed 44.1 % inhibition of AA-induced ear edema at an oral dose of 50 mg/kg. In an animal model of chronic inflammation, SAG clearly reduced the edematic response of 7 -day model of multiple treatment of TPA (38.1 % inhibition at 200 mg/kg/day). Furthermore, SAG (50-800 mg/kg/day) as well as S. deltoides extract (285 mg/kg/day) significantly inhibited prostaglandin $E_2$ production from the skin lesion of the animals of 7-day model. These results were well correlated with in vitro finding that SAG as well as S. deltoides extract reduced cyclooxygenase (COX)-1- and COX-2-induced prostanoid production, measured in mouse bone marrow-derived mast cells. Therefore, these results suggest that SAG possesses anti-inflammatory activity in vivo against acute as well as chronic inflammatory animal models at least in part by inhibition of prostaglandin production through COX-1/COX-2 inhibition. And COX inhibition of SAG is possibly contributed by S. deltoides extract among the ingredients. Although the anti-inflammatory potencies of SAG were less than those of currently used anti-inflammatory drugs, this formulation may have beneficial effect on inflammatory disorders as a neutraceutical.

• Journal of Power Electronics
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• v.10 no.2
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• pp.203-209
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• 2010

This paper describes the development of a three-phase line-interactive DVR with a new sag detection algorithm. The developed detection algorithm has a hybrid structure composed of an instantaneous detector and RMS-variation detectors. The source voltage passes through the sliding-window DFT and RMS calculator, and the instantaneous sag detector. If an instantaneous sag is detected, the RMS variation detector-1 is selected to calculate the RMS variation. The RMS variation detector-2 is selected when the instantaneous sag occurs under the operation of the RMS variation detector-1. The feasibility of the proposed algorithm is verified through computer simulations and experimental work with a prototype of a line-interactive DVR with a 3kVA rating. The line-interactive DVR with the proposed algorithm can compensate for an input voltage sag or an interruption within a 2ms delay. The developed DVR can effectively compensate for a voltage sag or interruption in sensitive loads, such as computers, communications equipment, and automation equipment.

• Advances in Energy Research
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• v.6 no.1
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• pp.55-73
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• 2019

The cost incurred by voltage sag effect in power networks has always been of important concern for discussions. Due to the environmental constraints, fossil fuel shortage crisis and low efficiency of conventional power plants, decentralized generation and renewable based DG have become trends in recent decades; because DGs can reduce the voltage sag effect in distribution networks noticeably; therefore, optimum allocation of DGs in order to maximize their effectiveness is highly important in order to maximize their effectiveness. In this paper, a new method is proposed for calculating the cost incurred by voltage sag effect in power networks. Thus, a new objective function is provided that comprehends technical standards as minimization of the cost incurred by voltage sag effect, active power losses and economic criterion as the installation and maintenance costs of DGs. Considering operational constraints of the system, the optimum allocation of DGs is a constrained optimization problem in which Lightning Attachment procedure optimization (LAPO) is used to resolve it and is the optimum number, size and location of DGs are determined in IEEE 33 bus test system and IEEE 34 bus test system. The results show that optimum allocation of DGs not only reduces the cost incurred by voltage sag effect, but also improves the other characteristics of the system.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.710-716
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• 1995

$\beta $-1, 4-D-arabinogalactanase isolated from alkalophilic Bacillus sp. HJ-12, approximate Mw 42 kDa, was generally stable in the range of pH 6-10 and below 50$\circ$C and its highest activity was observed at 60$\circ$C with pH 7-9. The isolated $\beta $-1, 4-D-arabinogalactanase specifically hydrolyzed $\beta $-1, 4-galactosyl linkage that is the major structure of soybean arabinogalactan (SAG) but not $\beta $-1, 3-galactosyl linkage of the other polysaccharides. K. was estimated as 0.67 mg/ml by the method of Hanes-Woolf plot. No metals and chemical reagents inhibited the enzyme activity but urea did. The active site of this enzyme assumed to be tryptophan residue. The hydrolysis products from SAG, assayed by gel chromatography, TLC and HPLC, were predominantly galactotetraose (Gal$_{4}$) and triose (Gal$_{3}$) with a small portion. $\beta $-1, 4-D-arabinogalactanase hydrolyzed ONPG as well as SAG, and the degree of hydrolysis of SAG was 15% which is lower than that by the other $\beta $-1, 4-galactanases from different sources. SAG treated with this enzyme resulted in the reduction of specific viscosity up to 70%.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.64 no.1
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• pp.29-34
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• 2015

This paper presents a method to select optimal device for mitigating voltage sags. The method is based on economic evaluation and voltage sag assessment involving sag duration as well as magnitude. The economic evaluation is performed by using the operation cost and economic benefit of the mitigation devices. The optimal device can be determined from the values of NPV (net present value) which is widely accepted in cost-benefit analysis. The proposed method can help sensitive customers to select optimal mitigation device. In this paper, the case study considering two sensitive customers was performed by using the proposed method.