• Clinical and Experimental Reproductive Medicine
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• v.27 no.4
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• pp.405-415
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• 2000

Objective: The present study was undertaken to synthesize a human Y chromosome specific probe and to confirm the usefulness of the probe for fluorescence in situ hybridization (FISH) in various types of human cells. Methods: An approximately 400 bp DNA fragment of the DYZ1 sequences was synthesized by PCR using digoxigenin labeled dUTP (dig-PCR). The fidelity of probe was tested by FISH for cultured and uncultured human lymphocytes, amniocytes, chorionic villus cells, embryos, sperms, and germ cells of seminiferous tubule. Results: The human Y chromosome specific probe hybridized specifically to Y chromosome of the cells that had been tested. This probe assigned to the Yq12 region where the DYZ1 repetitive sequence is concentrated. Conclusion: We have identified a human Y chromosome specific probe that hybridized specifically to the Y chromosome by FISH for various types of uncultured as well as cultured cells. Therefore FISH technique using human Y chromosome specific probe should be useful for clinical application as a diagnostic tool for the detection of human Y chromosome.

• Korean Journal of Pharmacognosy
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• v.53 no.1
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• pp.42-48
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• 2022

In order to maintain bone homeostasis, it is necessary to balance bone resorption and remodeling through the differentiation of osteoclasts that absorb old bone and osteoblasts that form new bone. However, bone resorption due to excessive osteoclast differentiation is a major cause of osteoporosis and controlling excessive osteoclast differentiation has been known as a treatment strategy for osteoporosis. Therefore, in this study, the effect of an ethanol extract of Sphaerotylus antarcticus Kirkpatrick, 1907 (SAE), polar-derived sponge with unknown biological activity, on the osteoclast differentiation process of RANKL-induced RAW264.7 cells and the generated ROS was evaluated. In the study results, SAE down-regulated the formation and function of RANKL-induced osteoclasts and osteoclast differentiation specific proteins, genes in a concentration-dependent manner. In addition, it was possible to confirm the result of restoring the lost antioxidant enzyme along with down-regulation of ROS generated by RANKL. Therefore, in this study, we propose the possibility of SAE as a potential regulator of osteoporosis due to excessive osteoclast differentiation and report the biological value of the diversity of marine-derived natural products by identifying the first biological activity against SAE that is not yet known.

• Transactions of the Korean hydrogen and new energy society
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• v.34 no.5
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• pp.447-455
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• 2023

SAE J2601, hydrogen fueling protocols, proposes two charging methods. The first is the table-based fueling protocol, and the second is the MC formula-based fueling protocol. Among them, MC formula-based fueling protocol calculates and supplies the target pressure and pressure ramp rate (PRR) using the pre-cooling temperature of the hydrogen and the physical parameters of the tank in the vehicle. The coefficient of the MC formula for deriving MC varies depending on the physical parameters of the tank in the vehicle. However, most studies use the MC coefficient derived from SAE J2601 as it is, despite the difference in the physical parameters of the tank applied to the study and the tank used to derive the MC coefficient from SAE J2601. In this study, the MC coefficient was derived by applying the hydrogen tank currently used, and the difference with the fueling performance using the MC coefficient proposed in SAE J2601 was verified. In addition, the difference was confirmed by comparing and analyzing the fueling performance of the table-based method currently used in hydrogen fueling stations and the MC formula-based method using MC coefficient derived in this study.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.6
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• pp.81-87
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• 2023

SAE J2601 and SAE J2799, the communication protocols between a hydrogen refueling station and a fuel cell electric vehicle, only cover hydrogen charging. In this paper, we measure the hydrogen detection, current, and voltage of a fuel cell electric vehicle and transmit the sensor data to the hydrogen refueling station by changing the WiFi protocol. A small-scale laboratory model was built using Raspberry Pi for sensing, controlling, and transmitting sensor data of a fuel cell electric vehicle. The sensor data was stored in the database of the hydrogen refueling station, and a dashboard was configured using Grafana to analyze the stored data. When hydrogen is detected, the dispenser valve of the hydrogen refueling station is locked. Then, we measured the average transmission delay according to the WiFi protocol. The results showed that IEEE 802.11a is the most suitable WiFi protocol for transmitting sensor data between the hydrogen refueling station and the fuel cell electric vehicle.

• Journal of Ocean Engineering and Technology
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• v.15 no.3
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• pp.93-99
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• 2001

Recently the steel parts used at automobils are required to be used under high stress more than ever before in need of the weight down. To achieve this requirement of a high strength steel, it must be necessary to decrease inclusion content and surface defect as like decarburization, surface roughness etc.. In this study, the surface conditions are measured to know the influence on fatigue properties by two cases of shot peening of two-stage shot peening and single-stage shot peening. And for this study, two kinds of spring steel (SAE 9254, DIN50CrV4) are used. This study shows the outstanding improvement of fatigue properties at the case of two-stage shot peening in the rotary bending fatigue test and this is assumed to be from (1) on low stress condition, th 1st stage shot peening is not affected by nonmetallic inclusion under metal.(2) it is possible that the 2nd stage shot peening increases the fatigue life and the high stress but that is affected by nonmetallic inclusion under metal. (3) so far beeasily DIN50CrV4 have made high stress. But, results also show fatigue failures originated at inclusion near surface, and this inclusion type is turned out to be a alumina of high hardness.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.6 no.2
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• pp.34-40
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• 2007

Antifatigue failure technology take an important part of current industries. Currently, the shot peening is used for removing the defect from the surface of steel and improving the fatigue strength on surface. Therefore, this paper the effect of compressive residual stress and corrosion of spring steel(SAE 5155)by shot-peening on fatigue crack growth characteristics in stress ratio(R=0.05)was investigated with considering fracture mechanics. By using the methods mentioned above, We arrived at the following conclusions. The fatigue crack growth rate(da/dN) of the shot-peening material was lower than that of the un-peening material. And in stage I, ${\Delta}Kth$, the threshold stress intensity factor, of the shot-peen processed material is high in critical parts unlike the un-peening material. Also m, fatigue crack growth exponent and number of cycle of the shot-peening material was higher than that of the un-peening material. That is concluded from effect of da/dN. Finally fracture of shot-peening material and un-peening material was identified and discussed in this study.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.4
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• pp.525-531
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• 2012

This study aims at the structural analysis of vibration and fatigue according to the configuration of engine mount. Maximum equivalent stress or deformation is shown at bracket or case respectively. As harmonic vibration analysis, the maximum displacement amplitude is happened at 4,000Hz. Among the cases of nonuniform fatigue loads, 'SAE bracket history' with the severest change of load becomes most unstable but 'Sample history' or 'Saw tooth' becomes most stable. In case of 'Sample history' or 'Saw tooth' with the average stress of 4,200MPa or 0MPa and the amplitude stress of -3,000MPa or 7MPa, the possibility of maximum damage becomes 70%. This stress state can be shown with 7 times more than the damage possibility of 'SAE bracket history' or 'SAE transmission'. The structural result of this study can be effectively utilized with the design on engine mount by investigating prevention and durability against its damage.

• Tribology and Lubricants
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• v.21 no.6
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• pp.249-255
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• 2005

In this paper, the friction characteristic of engine bearings has been analyzed in terms of a friction loss power, a minimum film thickness and an oil film pressure. This analysis has been focused on the fuel economy improvement with a low viscosity engine oil such as SAE 0W-40, which is used for a friction loss reduction and increased for a Diesel fuel economy. The friction loss power, the minimum oil film thickness and oil film pressure distribution for plain bearings of a Diesel engine are analyzed using an AVL's EXCITE program with a conventional engine oils of SAE 5W-40 and 10W-40, and a low viscosity engine oil of SAE 0W-40. The computed results indicate that a viscosity of engine oils is closely related to the friction loss power and the decreased minimum film thickness in which is a key parameter of a load carrying capacity of an oil film pressure distribution. When the low viscosity engine oil is supplied to engine bearings, it does not affect to the formation of a minimum oil film thickness. But the friction loss power has been significantly affected by low viscosity engine oil at a low operating temperature of 0. Based on the FEM computed results, the low viscosity engine oil at a low temperature range will be an important factor for an improvement of the fuel economy improvement.

• Transactions of the Korean Society of Automotive Engineers
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• v.20 no.4
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• pp.133-141
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• 2012

This study investigates structural and fatigue analyses of bike brake. Maximum equivalent stress of the model of mountain bike is 4 times as much as the model of general bike at static analysis. In cases of mountain and general bikes, maximum damage frequency at load of 'SAE bracket history' with the severest change of load becomes as much as 16 times than the most stable load of 'Sample history' among the nonuniform fatigue loads. In case of mountain bike, the possibility of maximum damage becomes 3% at the load of 'Sample history' with the average stress of 0 to $-3{\times}10^4$MPa and the amplitude stress of 0 to $10^4$MPa. In case of general bike, the possibility of maximum damage becomes 3% at the load of 'Sample history' with the average stress of 0 to $-0.8{\times}10^4$MPa and the amplitude stress of 0 to $0.2{\times}10^4$MPa. This stress state can be shown as 5 to 6 times more than the damage possibility of 'SAE bracket history' or 'SAE transmission'. The analysis result of this study can be effectively utilized for the safe design of bike brake.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.319-323
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• 2007

Wild grapes have been used as traditional medicinal use and alcoholic beverage production in Korea. The objective of this study is to improve antioxidant properties in Sae-muru by far infrared ray (FIR) treatment, with expecting potential benefits of FIR treatment for wild grape products during manufacturing processes. FIR treatment in berries induced increased content of catechin, epicatechin gallate, epigallocatechin gallate, gallic acid, rutin, ellagic acid, and resveratrol, while content of epicatechin and epigallocatechin was decreased. Although FIR treatment resulted either increased or decreased chemical component groups, presenting in HPLC chromatograms, antioxidant activity in Sae-muru extract was significantly increased by the FIR treatment. Our results suggest that FIR treatment should be an efficient process in the production of high content of bioactive molecules in Sae-muru.