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http://dx.doi.org/10.22889/KJP.2022.53.1.42

Inhibitory Effect of RANKL-Induced Osteoclast Differentiation and ROS Generation by Sphaerotylus antarcticus Extract  

Kim, Eun-Nam (College of Pharmacy, Chungnam National University)
Kang, Da Yeun (College of Pharmacy, Chungnam National University)
Trang, Nguyen Minh (College of Pharmacy, Chungnam National University)
Lee, Jun Hyuck (Research Unit of Cryogenic Novel Material, Korea Polar Research Institute)
Ko, Young Wook (Division of Life Sciences, Korea Polar Research Institute)
Kim, Sanghee (Division of Life Sciences, Korea Polar Research Institute)
Na, MinKyun (College of Pharmacy, Chungnam National University)
Jeong, Gil-Saeng (College of Pharmacy, Chungnam National University)
Publication Information
Korean Journal of Pharmacognosy / v.53, no.1, 2022 , pp. 42-48 More about this Journal
Abstract
In order to maintain bone homeostasis, it is necessary to balance bone resorption and remodeling through the differentiation of osteoclasts that absorb old bone and osteoblasts that form new bone. However, bone resorption due to excessive osteoclast differentiation is a major cause of osteoporosis and controlling excessive osteoclast differentiation has been known as a treatment strategy for osteoporosis. Therefore, in this study, the effect of an ethanol extract of Sphaerotylus antarcticus Kirkpatrick, 1907 (SAE), polar-derived sponge with unknown biological activity, on the osteoclast differentiation process of RANKL-induced RAW264.7 cells and the generated ROS was evaluated. In the study results, SAE down-regulated the formation and function of RANKL-induced osteoclasts and osteoclast differentiation specific proteins, genes in a concentration-dependent manner. In addition, it was possible to confirm the result of restoring the lost antioxidant enzyme along with down-regulation of ROS generated by RANKL. Therefore, in this study, we propose the possibility of SAE as a potential regulator of osteoporosis due to excessive osteoclast differentiation and report the biological value of the diversity of marine-derived natural products by identifying the first biological activity against SAE that is not yet known.
Keywords
S. antarcticus; RANKL; RAW264.7; Osteoclast; Osteoporosis; ROS;
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