• Title/Summary/Keyword: SA/CA

검색결과 168건 처리시간 0.024초

Sodium Nitroprusside로 유발한 HaCaT Cell의 Apoptosis (Induction of HaCaT Cell Apoptosis by Sodium Nitroprusside)

  • 박유리;문철;김사현;이평재
    • 대한임상검사과학회지
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    • 제47권3호
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    • pp.112-116
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    • 2015
  • 산화질소(Nitric Oxide, NO)는 생리학적, 병리학적으로 주요한 역할을 하고 있는 것으로 알려져 있다. 본 실험에서는 NO donor인 sodium nitroprusside (SNP)가 HaCaT 세포에서 apoptosis를 유도한다는 것을 DAPI염색과 PARP, caspase-3 단백질 절단을 western blot으로 확인하였다. SNP는 ER stress와 관련 있는 Bip, CHOP 유전자 발현에는 영향이 없었다. 최근 NAD+ 의존 deacetylase인 sirt1이 세포의 생존 및 사멸에 매우 중요한 단백질이라는 보고가 있다. 본 실험에서 SNP는 HaCaT 세포의 sirt1 유전자 발현을 감소시켰으며 이는 apoptosis와 관련이 있을 수 있다.

혼합미생물배양체를 이용한 폐활성슬러지 가용화 산물로부터 polyhydroxyalkanoate 생합성 (Biosynthesis of polyhydroxyalkanoate by mixed microbial cultures from hydrolysate of waste activated sludge)

  • 박태준;유영재;정동훈;이선희;이영하
    • 미생물학회지
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    • 제53권3호
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    • pp.200-207
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    • 2017
  • SA-TSA 복합구를 이용해 폐활성슬러지로부터 VFAs를 생산하고, 이를 발효 기질로 이용해 PHA를 생산하기 위한 실험을 수행하였다. 30%의 SA-TSA 복합구를 처리하였을 때 폐활성슬러지의 가용화 효율이 가장 효과적으로 일어나서 약 3,900 mg/L의 SCOD값을 얻었으며, acetic acid, propionic acid, iso-butyric acid 및 butyric acid를 주성분으로 하는 2,961 mg/L 농도의 VFAs를 얻었다. VFAs를 외부탄소원으로 이용하는 SBR 공정을 통해 PHA를 생합성하는 미생물을 농화배양하는 실험을 수행하고 PCR-DGGE로 분석한 결과, 우점 미생물로 Vibrio spp.와 Corynebacterium glutamicum이 확인되었다. 폐활성슬러지로부터 얻은 VFAs를 탄소원으로 사용하여 농화된 혼합미생물배양체를 회분배양한 결과, 건체량의 25.9%에 달하는 PHB를 얻었다. 본 연구결과는 SA-TSA 복합구를 이용하여 폐활성슬러지로부터 VFAs를 얻음으로써 폐슬러지 감량화 효과를 얻고, 또 혼합미생물배양체를 이용하여 VFAs를 바이오폴리머로 전환함으로써 경제성을 확보할 수 있는 새로운 생물공정의 가능성을 보여준다.

토끼 동방결절 활동전압에 대한 Na, K, Ca 및 Mg 이온의 영향 (Effect of Na, K, Ca and Mg ions on the Action Potential of the Sinoatrial Node in the Rabbit)

  • 이정렬;엄융의
    • Journal of Chest Surgery
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    • 제18권1호
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    • pp.1-6
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    • 1985
  • Isolated sinus node cells of the rabbit were used to assess the effects of extracellular Na, K, Ca and Mg concentrations on cardiac pacemaker activity. With intracellular glass micro-electrodes spontaneous action potentials of SA node were recorded and the effects of various ions and their blockers were analyzed in terms of the cycle length, the amplitude and the duration of action potentials, the results obtained were as follows. 1. Sodium reduction [up to 30%] decreased the amplitude of action potential and lengthened the cycle length. TTX, specific blocker of Na channel slightly lengthened the cycle length. 2. Increasing potassium ion concentration, the duration of action potential decreased and the frequency increased in 6mM, however, spontaneous action potential was stopped in 24 mM. Barium ion known to be decreasing K conductance increased the duration of action potential but no significant change in the cycle length was noticed. 3. Calcium ion has shortening effect on the duration and the cycle length of action potential but not with dose-dependent manner. Cadmium ion .[0.02mM] lengthened cycle length and the duration of action potential. 4. Increasing the concentration of magnesium ion the cycle length was lengthened, significantly.

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도석을 점결제로 상용한 하수슬러지 인공경량골재의 개발에 관한 실험적 연구 (An Experimental Study on the Development of Sewage Sludge Artificial Light-weight Aggregate Using Pottery Stone)

  • 정의승;사순헌;지석원;서치호
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2010년도 춘계 학술논문 발표대회 1부
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    • pp.91-94
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    • 2010
  • The purpose of this study is to produce artificial lightweight aggregate. The properties of aggregate are deducted by analysing the plasticity of aggregate according to the addictive contents of $CaCO_3$ and $Al_2O_3$ on constant plastic temperature($1150^{\circ}C{\sim}1160^{\circ}C$) and the specific gravity, the percentage of water absorbtion. The density on the temperature of $1150^{\circ}C{\sim}1160^{\circ}C$ which results from that the plastic temperature of pottery stone is decreased by increasing the addictive contents of $CaCO_3$ and $Al_2O_3$ manufacturing artificial light weight aggregate using pottery stone is included in the arrange of light weight aggregate density. And the percentage of water absorbtion is 4.2~14% which is similar to or lower than existing artificial light weight aggregate. The unit volume weight is in inverse proportion to density and to increase addictive contents of flux.

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$(Sr_{0.9}Ca_{0.1})TiO_{3}$ 세라믹 박막의 열처리온도에 따른 특성 (Properties with Annealing Temperature of $(Sr_{0.9}Ca_{0.1})TiO_{3}$ Ceramic Thin Film)

  • 소병문;조춘남;신철기;김진사;김충혁
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제51권11호
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    • pp.526-530
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    • 2002
  • The $(Sr_{0.9}Ca_{0.1})TiO_3(SCT)$ thin films were deposited on Pt-coated electrode (Pt/TiN/$SiO_2$/Si) using RF sputtering method. The composition of SCT thin film deposited on Si substrate at room temperature is close to stoichiometry(1.081 in A/B ratio). The maximum dielectric constant of SCT thin film was obtained by ammealing at $600^{\cric}C$. The temperature dependence of dielectric loss showed a value within 0.02 in temperature ranges of -80 ${\sim}$ +90$^{\circ}C$. The capacitance characteristics showed a stable value within ${\pm}$4%. The drastic decrease of dielectric constant and increase of dielectric loss in SCT thin films was observed at the frequency above 200kHz.

$(Sr{\cdot}Ca)TiO_{3}$ 세라믹스의 용량-전압 특성 (Capacitive-Voltage properties of$(Sr{\cdot}Ca)TiO_{3}$ Ceramics)

  • 강재훈;최운식;김충혁;김진사;박용필;송민종
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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    • pp.34-37
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    • 2001
  • In this study, the capacitance-voltage properties of $(Sr_{1-x}\cdot Ca_x)TiO_3(0.05{\leq}x{\leq}0.20)$-based grain boundary layer ceramics were investigated. The ceramics were fabricated by the conventional mixed oxide method. The sintering temperature and time were $1480\sim1500^{\circ}C$ and 4 hours. respectively. The 2nd phase formed by the thermal diffusion of CuO from the surface leads to very excellent dielectric properties, that is, ${\varepsilon}_r$ >50000, tan$\delta$ <0.05, ${\Delta}C$ < ${\pm}10%.$ The capacitance is almost unchanged below about 20[V] but it decreases slowly about 20[V]. The results of the capacitance-voltage properties indicated that the grain boundary was composed of the continuous insulating layers.

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Pt/ST/Pt 소자 구조의 박막증착 및 특성 (Deposition and Properties of Pt/ST/Pt Thin Film Structure)

  • 김진사;조춘남;오용철;신철기;송민종;소병문;최운식;김충혁
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
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    • pp.472-473
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    • 2007
  • The $(Sr_{1-x}Ca_x)TiO_3$(ST) thin films are deposited on Pt-coated electrode(Pt/TiN/$SiO_2$/Si) using RF sputtering method with substitutional contents of Ca. The maximum grain of thin films is obtained by substitution of Ca at 15[mol%]. Also, the composition of ST thin films were closed to stoichiometry(1.081~1.117 in A/B ratio). The dielectric constant changes almost linearly in temperature ranges of -80~+90[$^{\circ}C$]. The current-voltage characteristics of ST15 thin films showed the increasing leakage current as the measuring temperature increases.

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수용성 치자(水溶性 梔子)(Gardenia jasminoides열매)색소(色素)를 첨가(添加)한 배지(培地)에 배양(培養)한 각종(各種) 효모균류(酵母菌類) 균집락(菌集落)의 형태(形態) (Colonial Morphology of Yeasts Grown on the Medium Containing Pigments Extracted from the Fruits of Gardenia jasminoides)

  • 김상재;김신옥;정 숙
    • 한국균학회지
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    • 제19권2호
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    • pp.136-142
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    • 1991
  • 수용성 치자(水溶性 梔子)(Ga. jasminoides의 열매)색소(色素)(등황색)(橙黃色)를 냉수(冷水)로 추출하여 함유시킨 yeast morphology agar(Difco)에 각종 효모균류(酵母菌類)를 배양하여 그들의 균집락(菌集落) 색깔변화를 관찰하여 다음과 같은 결과를 얻었다. 치자배지(梔子培地)에서 증식(增殖)한 Ca. guilliermondii 와 Ca. lusitaniae 의 균집락(菌集落)은 $28^{\circ}C$에서 3일이내에 군청색(群靑色)을 띠었고, Ca. tropicalis와 Ca. viswanathii 등은 옅은 회색(灰色)을 띠었는데 Ca. viswanathii는 7일후에 군청색(群靑色)으로 변했다. Ca. krusei와 To. glabrata 등은 $1{\sim}2$주후 회색(灰色)내지 옅은 회청색(灰靑色)을 띠었고 Sa. cerevi-siae는 연두색을 띠었다. 그러나 Ca. albicans와 Ca. parapsilosis 등은 색깔변화가 없었고, 적색(赤色) 균집락(菌集落)의 Rhodotorula sp.도 변화가 없었다. 그러나 Cr. neofor-mans는 갈색(褐色) 내지 자갈색(紫褐色) 균집락(菌集落)을 형성해 다른 모든 균종(菌種)과 뚜렷하게 구별되어 기존(旣存) 선별동정배지(選別同定培地)인 caffeic acid 또는 dopamine배지(培地)를 대체할 수 있을 뿐 아니라 몇몇 Candida spp.의 특정적 색깔을 관찰할 수 있어서 치자배지(梔子培地)가 임상검체(臨床檢體)로부터 효모균종(酵母菌種)의 분리배양(分離培養)에 매우 유용하다고 생각한다.

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부산시 해안 및 내륙지역에서 광화학 오염물질의 농도 차이에 영향을 주는 화학 및 기상조건 분석 (Analysis of Chemical and Meteorological Effects on the Concentration Difference of Photochemical Air Pollutants between Coastal and Inland Regions in Busan)

  • 송상근;손장호
    • 한국환경과학회지
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    • 제17권10호
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    • pp.1169-1182
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    • 2008
  • The chemical and meteorological effects on the concentration variations of air pollutants ($O_3$ and its precursors) were evaluated based on ground observation data in coastal and inland regions, Busan during springs and summers of 2005-2006. For the purpose of this study, study areas were classified into 5 categories: coastal area (CA), industrial area (IA), downtown area (DA), residential area (RA), and suburban area (SA). Two sites of Dongsam (DS) and Yeonsan (YS) were selected for the comparison purpose between the coastal and inland regions. $O_3$ concentrations in CA and SA were observed to be highest during spring (e.g., 40 ppb), whereas those in DA and RA were relatively low during summer (e.g., $22\sim24$ ppb). It was found that $O_3$ concentrations in IA were not significantly high although high VOCs (especially toluene of about 40 ppb) and $NO_x$ ($\geq$ 35 ppb) were observed. On the other hand, the concentration levels of $O_3$ and $PM_{10}$ at the DS site were significantly higher than those at the YS site, but $NO_x$ was slightly lower than that at the YS site. This might be caused by the photochemical activity and meteorological conditions (e.g., sea-land breeze and atmospheric stagnance). When maximum $O_3$ (an index of photochemical activity) exceeds 100 ppb, the contribution of secondary $PM_{10}\;((PM_{10})_{SEC})$ to total observed $PM_{10}$ concentrations was estimated up to 32% and 17% at the DS and YS sites, respectively. In addition, the diurnal variations of $(PM_{10})_{SEC}$ at the DS site were similar to those of $O_3$ regardless of season, which suggests that they are mostly secondary $PM_{10}$ produced from photochemical reactions.