• 동아시아식생활학회지
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• 제18권6호
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• pp.981-988
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• 2008

This study was conducted to develop functional black soy sauce with (S2) and without (S3) an outer skin and then compare these products to a control soy sauce (S1). In addition, the effects of different fermentation periods on the pH, buffering power, titratable acidity, total acidity, salt content, and browning and Lab value were evaluated. Furthermore, the antioxidative activities of the black soy sauce were compared to those of the control soy sauce based on the total phenolic compounds and free radical scavenging activity, including the 1,1-diphenyl-2-picryl-hydrazil (DPPH) scavenging activity and the thiobarbituric acid value (TBA value). The pH and buffering power of S2 were lower than of S1 and S3, while the titratable acidity and total acidity were higher. The salt content of all samples decreased after 60 days of fermentation, after which it increased slightly for up to 180 days. Additionally, the browning intensity of all samples increased as the fermentation periods increased, with the browning intensity at 420 nm of S1 being the highest followed by S3 and S2. After 150 days of fermentation, the L value of S1 was higher than that of S2 and S3, but the while a value of S2 was higher than those of S1 and S3 and was increased as the fermentation periods. Moreover, the b value of S1 was the highest at the end of the fermentation period, followed by the b values of S3 and S2. The amount of total phenolics in S1 was greatest, followed by S2 and S1. Conversely, the DPPH radical scavenging activity of S2 was the highest, followed by S3 and S1. Finally, the TBA value increased rapidly from day 30 to day 180 of the fermentation period, and the TBA value of S2 was lower than those of S1 and S3.

• BMB Reports
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• 제43권4호
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• pp.233-244
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• 2010

Parkinson's disease (PD) is the second most common neurodegenerative disease, and 5-10% of the PD cases are genetically inherited as familial PD (FPD). LRRK2 (leucine-rich repeat kinase 2) was first reported in 2004 as a gene corresponding to PARK8, an autosomal gene whose dominant mutations cause familial PD. LRRK2 contains both active kinase and GTPase domains as well as protein-protein interaction motifs such as LRR (leucine-rich repeat) and WD40. Most pathogenic LRRK2 mutations are located in either the GTPase or kinase domain, implying important roles for the enzymatic activities in PD pathogenic mechanisms. In comparison to other PD causative genes such as parkin and PINK1, LRRK2 exhibits two important features. One is that LRRK2's mutations (especially the G2019S mutation) were observed in sporadic as well as familial PD patients. Another is that, among the various PD-causing genes, pathological characteristics observed in patients carrying LRRK2 mutations are the most similar to patients with sporadic PD. Because of these two observations, LRRK2 has been intensively investigated for its pathogenic mechanism (s) and as a target gene for PD therapeutics. In this review, the general biochemical and molecular features of LRRK2, the recent results of LRRK2 studies and LRRK2's therapeutic potential as a PD target gene will be discussed.

• Communications for Statistical Applications and Methods
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• 제6권1호
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• pp.69-76
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• 1999

A new statistic {{{{ {d }`_{2 } ^{s } }}}} is introduced for constructing co ntrol limits. It is easier and more convienient than d2 We will show the characteristic of {{{{ {d }`_{2 } ^{s } }}}} and evaluate {{{{ {d }`_{2 } ^{s } }}}} through average run length(ARL).

• 대한전기학회논문지
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• 제41권6호
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• pp.687-693
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• 1992

To improve dielectric, piezoelectric and temperature stability in 0.50Pb(SnS11/2TSbS11/2T)OS13T - 0.35PbTiOS13T - 0.60PbZrOS13T + 0.4[wt%]MnOS12T piezoelectric ceramics which is used for surface acoustic wave devices, CrS12TOS13T was added and the specimens were fabricated by Hot Press method, and their characteristics were measured with CrS12TOS13T addition. From the results, in the specimen added by 0.2[wt%]CrS12TOS13T, dielectric constant and mechanical quality factor were 380 and 2307, respectively, and it was suited for surface acoustic wave device and the temperature coefficient of the resonant frequency in the specimen added by 0.4[wt%]CrS12TOS13T was the least value of 74.96[ppm/$^{\circ}C$].

• 생명과학회지
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• 제27권12호
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• pp.1403-1409
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• 2017

본 연구에서는 lignocellulosic biomass (xylose)의 부가가치를 높이고 효율적인 활용을 위해 xylitol dehydrogenase를 Saccharomyces cerevisiae 숙주세포에서 분비 생산하고자 하였다. 먼저 S. cerevisiae와 Pichia stipitis유래 XYL2 유전자(S.XYL2 and P.XYL2 gene)의 발현 시스템을 구축하기 위하여 GAL10 promoter와 ADH1 promoter 하류에 각각 mating factor ${\alpha}$ ($MF{\alpha}$) signal sequence와 XYL2유전자를 가진 $pGMF{\alpha}-S.XYL2$, $pGMF{\alpha}-P.XYL2$, $pAMF{\alpha}-S.XYL2$와 $pAMF{\alpha}-P.XYL2$ plasmid를 구축하였다. 각각의 plasmid는 S. cerevisiae $SEY2102{\Delta}trp1$ 균주에 형질전환되었고, 생산된 xylitol dehydrogenase의 활성을 조사해 본 결과, GAL10 promoter가 ADH1 promoter보다 XYL2유전자의 발현에 더욱 적합함을 확인 할 수 있었다. 또한 P. stipitis 유래의 xylitol dehydrogenase 효소 활성이 S. cerevisiae 유래의 효소 활성보다 2배 이상 더 높았으며, 활성의 증가를 위해 두 유전자 모두 cofactor로 $NAD^+$에 의존한다는 것을 확인하였다. 재조합 유전자가 가지는 분비서열에 의해 $SEY2102{\Delta}trp1/pGMF{\alpha}-P.XYL2$ 균주에서 xylitol dehydrogenase의 약 77%는 periplasmic space로 분비 발현되었음을 알 수 있었다. 또한 재조합 xylitol dehydrogenase의 효율적인 생산을 위해 탄소원의 영향을 조사해본 결과, glucose 단독보다 glucose와 xylose를 혼합 배양한 경우에서 효소활성이 최대 41% 정도 증가되었음을 확인 할 수 있었다. 본 연구에서 최적화한 발현 시스템 및 배양 조건은 xylose 뿐만 아니라 다양한 biomass를 이용한 유용물질 생산을 위한 관련 단백질의 발현 분비시스템 구축 및 대량생산에도 응용될 수 있을 것이라 생각된다.

• 대한전자공학회논문지SD
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• 제45권8호
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• pp.67-74
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• 2008

다양한 하드웨어 공유 및 최적화 방법을 적용하여 저면적/고성능 AES(Advanced Encryption Standard) 암호/복호 프로세서를 설계하였다. 라운드 변환블록 내부에 암호연산과 복호연산 회로의 공유 및 재사용과 함께 라운드 변환블록과 키 스케줄러의 S-Box 공유 등을 통해 회로 복잡도가 최소화되도록 하였으며, 이를 통해 S-Box의 면적을 약 25% 감소시켰다. 또한, AES 프로세서에서 가장 큰 면적을 차지하는 S-Box를 합성체 $GF(((2^2)^2)^2)$ 연산을 적용하여 구현함으로써 $GF(2^8)$ 또는 $GF((2^4)^2)$ 기반의 설계에 비해 S-Box의 면적이 더욱 감소되도록 하였다. 64-비트 데이터패스의 라운드 변환블록과 라운드 키 생성기의 동작을 최적화시켜 라운드 연산이 3 클록주기에 처리되도록 하였으며, 128비트 데이터 블록의 암호화가 31 클록주기에 처리되도록 하였다. 설계된 AES 암호/복호 프로세서는 약 15,870 게이트로 구현되었으며, 100 MHz 클록으로 동작하여 412.9 Mbps의 성능이 예상된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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• pp.193-196
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• 2001

Single phase $CuInS_2$ thin film with the highest diffraction peak (112) at diffraction angle $(2\theta)$ of $27.7^{\circ}$ and the second highest diffraction peak (220) at diffraction angle $(2\theta)$ of $46.25^{\circ}$ was well made with chalcopyrite structure at substrate temperature of $70^{\circ}C$, annealing temperature of $250^{\circ}C$, annealing time of 60 min. The $CuInS_2$ thin film had the greatest grain size of $1.2{\mu}m$ and Cu/In composition ratio of 1.03. Lattice constant of a and c of that $CuInS_2$ thin film was 5.60 A and 11.12 A respectively. Single phase $CuInS_2$ thin films were accepted from Cu/In composition ratio of 0.84 to 1.3. P-type $CuInS_2$ thin films were appeared at over Cu/In composition ratio of 0.99. Under Cu/In composition ratio of 0.96, conduction types of $CuInS_2$ thin films were n-type. Also, fundamental absorption wavelength, the absorption coefficient and optical energy band gap of p-type $CuInS_2$ thin film with Cu/In composition ratio of 1.3 was 837 nm, $3.0{\times}104cm^{-1}$ and 1.48 eV respectively. When Cu/In composition ratio was 0.84, fundamental absorption wavelength, the absorption coefficient and optical energy band gap of n-type $CuInS_2$ thin film was 821 nm, $6.0{\times}10^4cm^{-1}$ and 1.51 eV respectively.

• 미생물학회지
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• 제39권3호
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• pp.123-127
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• 2003

Sphingomonas chungbuken교 DJ77은 phenanthrene을 단일 탄소원과 에너지원으로 이용하여 살아갈 수 있다. pUPXS는 phenanthrene과 naphthalene분해를 위해 필요한 2-hydroxychromene-2-carboxylate (HCCA) isomerase를 암호화하는phnS유전자를 포함한다. 본 논문에서는 phnS유전자가 포함된 3271 bp의 염기 서열을 결정하였다. 이 유전자는 ATG를 개시코돈으로 사용하며, TAA를 종결 코돈으로 사용하고 있다. 또한 개시 코돈의 5 bp앞쪽으로 GGAA라는 ribosomal binding site를 갖는다. phnS는 총 594 bP의 open reading frame을 포함하며,158개의 아미노산으로 구성되었다. phns를 구성하는 아미노산서열은 S. aromaticivorans F199의 유사서열과 87％의 유사성을 나타냈다. phns 유전자는 biphenyl dioxygenase를 구성하는 2,3-dihydroxybiphenyl 1,2-dioxygenase를 암호화하는 phnQ와 ferredoxin를 암호화 하는 phnR과 같은 operon을 이루며, 이들 유전자의 downstream에 위치하고 있다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.193-196
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• 2001

Single phase CuInS$_2$ thin film with the highest diffraction peak (112) at diffraction angle (2$\theta$) of 27.7$^{\circ}$ and the second highest diffraction peak (220) at diffraction angle (2$\theta$) of 46.25$^{\circ}$ was well made with chalcopyrite structure at substrate temperature of 70 $^{\circ}C$, annealing temperature of 25$0^{\circ}C$, annealing time of 60 min. The CuInS$_2$ thin film had the greatest grain size of 1.2 ${\mu}{\textrm}{m}$ and Cu/In composition ratio of 1.03. Lattice constant of a and c of that CuInS$_2$ thin film was 5.60 $\AA$ and 11.12 $\AA$ respectively. Single phase CuInS$_2$ thin films were accepted from Cu/In composition ratio of 0.84 to 1.3. P-type CuInS$_2$ thin films were appeared at over Cu/In composition ratio of 0.99. Under Cu/In composition ratio of 0.96, conduction types of CuInS$_2$ thin films were n-type. Also, fundamental absorption wavelength, the absorption coefficient and optical energy band gap of p-type CuInS$_2$ thin film with Cu/In composition ratio of 1.3 was 837 nm, 3.0x10 $^4$ $cm^{-1}$ / and 1.48 eV respectively. When CuAn composition ratio was 0.84, fundamental absorption wavelength, the absorption coefficient and optical energy band gap of n-type CuInS$_2$ thin film was 821 nm, 6.0x10$^4$ $cm^{-1}$ / and 1.51 eV respectively.

• 한국작물학회지
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• 제24권4호
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• pp.62-66
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• 1979

고려인삼의 배 및 식물조직편의 탈분화와 Callus의 유기 및 생장에 미치는 2,4-D 및 Bengyladenine의 효과를 구명하고 Callus와 유기 및 생장에 필요한 2,4-D의 최저 및 최적농도를 밝히고져 본연구를 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 2,4-D를 0.1mg/l 첨가한 배지상에서는 인나의 배나 식물편에서 Callus가 전혀 유기되지 않았으나 0.5mg/l 처리한 배지상에서는 전체배의 \frac{1}{3} 에서 Callus가 유기되었으며 식물편에서는 모두 약간의 Callus가 유기되었다. 2. Callus의 유기는 2,4-D를 5.0mg/l처리한 배지에서 가장 빨랐으나 Callus의 생장량은 1.0~2.0mg/l의 2,4-D를 첨가한 배지에서 현저히 않은 경향이었다. 3. 2,4-D를 0.5mg/l첨가한 배지에 이식한 배에서는 자엽이 Callus화되어 Callus가 어느 정도 성장한 후 상배축의 기부에서 5~6개이상의 구슬모양의 신아가 형성되었다. 4. Benzyladenine은 근, 경 및 엽의 생장을 억제하고 자엽의 신장비대를 조장하였으며 고농도의 BenByladenine은 Callus의 유기를 억제하는 효과가 현저하였다. 5. 2,4-D 0.1mg/l 및 Benzyladenine 1.0mg/l를 조합처리한 배지상에 이식된 배의 상배축 기부에서도 5개이상의 구슬모양의 신아가 형성되었다.