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Sequence and phylogenetic analysis of the phnS gene encoding 2-hydroxychromene-2-carboxylate isomerase in Sphingomonas chungbukensis DJ77  

엄현주 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
강민희 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
김영필 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
김성재 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
김영창 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
Publication Information
Korean Journal of Microbiology / v.39, no.3, 2003 , pp. 123-127 More about this Journal
Abstract
Sphingomonas chungbukensis DJ77 is able to metabolize phenanthrene as the sole carbon and energy source. The plasmid pUPX5 includes phnS gene encoding 2-hydroxychromene-2-carboxylate (HCCA) isomerase, which is needed for phenanthrene and naphthanene degradation. We determined the nucleotide sequence of DNA fragment of 3271 bp which included the phnS gene. The fragment included an open reading frame of 594 bp which has ATG initiation codon and TAA termination codon and GGAA ribosomal binding site. The predicted amino acid sequence of the enzyme consists of 198 amino acids. The deduced amino acid sequence of the phnS enzyme exhibited 94% identity with that of the corresponding enzyme in Sphingomonas aromaticivorans F199. The phnS gene is located downstream and in the same operon as phnQ and phnR, encoding a 2,3-dihydroxybiphenyl 1,2-dioxygenase and a ferredoxin component of biphenyl dioxygenase, respectively.
Keywords
Sphingomonas chungbukensis DJ77; phnS; 2-hydroxychromene-2-carboxylate isomerase;
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Times Cited By KSCI : 2  (Citation Analysis)
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