• Journal of Life Science
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• v.19 no.8
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• pp.1039-1046
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• 2009

The cytochrome P450s (P450s) metabolizing natural products are among the most versatile biological catalysts known in plants, but knowledge of the structural basis for their broad substrate specificity has been limited. The activity of p-coumarate 3-hydroxylase (C3H) is thought to be essential for the biosynthesis of lignin and many other phenylpropanoid pathway products in plants however, all attempts to express and purify the protein corresponding C3H gene have failed. As a result, no conditions suitable for the unambiguous assay of the enzyme are known. The detailed understanding of the mechanism and substrate-specificity of C3Hdemands a method for the production of active protein on the milligram scale. We have developed a bacterial expression and purification system for the plant C3H, which allows for the quick expression and purification of active wild-type C3H via introduction of combinational mutagenesis. The modified cytochrome P450 C3H ($C3H_{mod}$) could be purified in the absence of detergent using immobilized metal affinity chromatography and size exclusion chromatography following extraction from isolated membranes in a high salt buffer and catalytically activated. This method makes the use of isotopic labeling of C3H for NMRstudies and X-ray crystallography practical, and is also applicable to other plant cytochrome P450 proteins.

• Journal of Soil and Groundwater Environment
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• v.11 no.6
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• pp.69-75
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• 2006

Improper disposal of petroleum and spills from underground storage tanks have created large areas with highly toxic contamination of the soil and groundwater. Methyl tert-butyl ether (MTBE) is widely used as a fuel additive because of its advantageous properties of increasing the octane value and reducing carbon monoxide and hydrocarbon exhausts. However, MTBE is categorized as a possible human carcinogen. This research investigated the Modified Photo-Fenton system which is based on the Modified Fenton reaction and UV light irradiation. The Modified Fenton reaction is effective for MTBE degradation near a neutral pH, using the ferric ion complex composed of a ferric ion and environmentally friendly organic chelating agents. This research was intended to treat high concentrations of MTBE; thus, 1,000 mg/L MTBE was chosen. The objectives of this research are to find the optimal reaction conditions and to elucidate the kinetic and mechanism of MTBE degradation by the Modified Photo-Fenton reaction. Based on the results of experiments, citrate was chosen among eight chelating agents as the candidate for the Modified Photo-Fenton reaction because it has a relatively higher final pH and MTBE removal efficiency than the others, and it has a relatively low toxicity and is rapidly biodegradable. MTBE degradation was found to follow pseudo-first-order kinetics. Under the optimum conditions, [$Fe^{3+}$] : [Citrate] = 1 mM: 4 mM, 3% $H_2O_2$, 17.4 kWh/L UV dose, and initial pH 6.0, the 1000 ppm MTBE was degraded by 86.75% within 6 hours and 99.99% within 16 hours. The final pH value was 6.02. The degradation mechanism of MTBE by the Modified Photo-Fenton Reaction included two diverse pathways and tert-butyl formate (TBF) was identified to be the major degradation intermediate. Attributed to the high solubility, stability, and reactivity of the ferric-citrate complexes in the near neutral condition, this Modified Photo-Fenton reaction is a promising treatment process for high concentrations of MTBE under or near a neutral pH.

• Proceedings of the KIEE Conference
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• 2001.11b
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• pp.121-123
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• 2001

This paper presents a fault location algorithm using iterative method at unbalance condition for distribution feeder systems. Distribution feeders include single phase and three phase laterals. The proposed algorithm achieves a high accuracy by continuously updating voltage and current phasor using the phase components and admittance load model.

• Spatial Information Research
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• v.14 no.2 s.37
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• pp.223-234
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• 2006

In order to examine spatio-temporal characteristics of coastal water quality, we applied GIS spatial analysis to the water quality data collected from observation points located on Korean coastal area during 1997$\sim$2004. The water quality parameters measured included: chlorophyll-a, pH, DO, COD, SS, dissolved inorganic nitrogen, dissolved inorganic phosphorous, salinity, temperature. The water quality data used in this paper was obtained only at selected sites even though they are potentially available at any location in a continuous surface. Thus, it is necessary to estimate the values at unsampled locations so as to analyze spatial distribution patterns of coastal water quality, Owing to this reason, we applied IDW(inverse distance weighted) interpolation method to water quality data and evaluated the usefulness of IDW method. After IDW interfolation method was applied, we divided the Korean coastal area into 46 sections and examined spatio-temporal patterns of each section using GIS visualization technique. As a result of evaluation, we can blow that IDW interpolation and GIS are useful for understanding spatial and temporal distribution characteristics of coastal water quality data which is collected from a wide area far many years.

• Proceedings of the KIPE Conference
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• 2007.11a
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• pp.202-205
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• 2007

현재 태양광, 풍력 및 연료전지 즉, 신재생에너지를 많이 사용하게 됨으로써 이를 계통과 병렬로 연결하여 쓰고 있다. 그러나 계통에 연결할 경우 여러 가지 문제가 발생할 수가 있는데 그중에서 대표적으로 단독운전을 들 수가 있다. 이를 방지하기 위한 검출기능은 시스템의 안전성 및 전기적 안전성 측면에서 매우 중요하다. 본 연구에서는 단독운전 검출을 위한 RPV 방식 및 제어알고리즘을 제안하였으며, 계통의 전압 위상을 기준으로 전류위상각도에 따른 주파수 변동 특성을 해석하였다. 해석 및 제안된 방법의 타당성은 EMTP 및 Matlab 을 이용하여 검증하였다.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.11
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• pp.103-106
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• 1996

In this paper, we proposed the design and algorithm of force reflection joystick which control mobile robot as a rehabilitation assistance system. The disabled persons are poor at joystick control because of hand vibration and clumsiness in operating it. These problems bring tasks which concerned with operator's safety So there is required technique which prevent collision with wall or obstacles. One of these solution is force reflection joystick which disturb that robot is closed to the wall. To confirm this way, we experimented and simulated with force reflection joystick which attached torque controller.

• Journal of Soil and Groundwater Environment
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• v.10 no.1
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• pp.58-64
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• 2005

Several tests were conducted to determine the optimum operational conditions of soil washing techniques for floe-forming arsenic-contaminated soils, collected from D abandoned Iron-mine in Korea. The optimum cut-off size was 0.15 mm $(sieve\;\#100)$, about $94\%$ of the mass of soils. Both sodium hydroxide and hydrochloric acid were effective to remove arsenic and the optimum mixing ratio (soil [g] : washing solution [mL]) was 1:5 for both washing agents. Arsenic concentrations, determined by KST Methods, for the dried floe solids obtained from flocculation at pH 5-6 were $990\~1,086\;mg/kg$ dry solids, which were higher concentrations than at the other pH values. Therefore, batch tests for sequential washings with or without removing floc were conducted to find the enhancement of washing efficiencies. After removing floe with 0.2 M HCl, sequential washings of 1 M HCl followed by 1 M NaOH showed the best results (15 mg/kg dry soil). The arsenic concentrations of washing effluent from each washing step were about $2\~3\;mg/L$. However, when these acidic and basic effluents were mixed together, arsenic concentration was decreased to be less than $50\;{\mu}g/L$, due to the pH condition of coagulation followed by precipitation for arsenic removal.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.2
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• pp.28-34
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• 2011

In this paper, we present integer and fractional motion estimation IP for H.264/AVC encoder by hardware-oriented algorithm. In integer motion engine, the reference block is used to share for consecutive current macro blocks in parallel processing which exploits data reusability and reduces off-chip bandwidth. In fractional motion engine, instead of two-step sequential refinement, half and quarter pel are processed in parallel manner in order to discard unnecessary candidate positions and double throughput. The H.264/AVC motion estimation chip is fabricated on a MPW(Multi-Project Wafer) chip using the chartered $0.18{\mu}m$ standard CMOS 1P5M technology and achieves high throughput supporting HDTV 720p 30 fps.

• Journal of Soil and Groundwater Environment
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• v.12 no.2
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• pp.27-36
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• 2007

A recent study showed that MTBE can be degraded by Fenton's Reagent (FR). The treatment of MTBE with FR, however, has a definite limitation of extremely low pH requirement (optimum pH $3{\sim}4$) that makes the process impracticable under neutral pH condition on which the ferrous ion precipitate forming salt with hydroxyl anion, which result in the diminishment of the Fenton reaction and incompatible with biological treatment. Consequently, this process using only FR is not suitable for in-situ remediation of MTBE. In order to overcome this limitation, modified Fenton process using NTA, oxalate, and acetate as chelating reagents was introduced into this study. Modified Fenton reaction, available at near neutral pH, has been researched for the purpose of obtaining high performance of oxidation efficiency with stabilized ferrous or ferric ion by chelating agent. In the MTBE degradation experiment with modified Fenton reaction, it was observed that this reaction was influenced by some factors such as concentrations of ferric ion, hydrogen peroxide, and each chelating agent and pH. Six potential chelators including oxalate, succinate, acetate, citrate, NTA, and EDTA were tested to identify an appropriate chelator. Among them, oxalate, acetate, and NTA were selected based on their remediation efficiency and biodegradability of each chelator. Using NTA, the best result was obtained, showing more than 99.9% of MTBE degradation after 30 min at pH 7; the initial concentration of hydrogen peroxide, NTA, and ferric ion were 1470 mM, 6 mM, and 2 mM, respectively. Under the same experimental condition, the removal of MTBE using oxalate and acetate were 91.3% and 75.8%, respectively. Optimum concentration of iron ion were 3 mM using oxalate which showed the greatest removal efficiency. In case of acetate, $[MTBE]_0$ decreased gradually when concentration of iron ion increased above 5 mM. In this research, it was showed that modified Fenton reaction is proper for in-situ remediation of MTBE with great efficiency and the application of chelatimg agents, such as NTA, was able to make the ferric ion stable even at near neutral pH. In consequence, the outcomes of this study clearly showed that the modified Fenton process successfully coped with the limitation of the low pH requirement. Furthermore, the introduction of low molecular weight organic acids makes the process more available since these compounds have distinguishable biodegradability and it may be able to use natural iron mineral as catalyst for in situ remediation, so as to produce hydroxyl radical without the additional injection of ferric ion.

• Investigative Magnetic Resonance Imaging
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• v.17 no.2
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• pp.123-132
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• 2013

Purpose : We report the results of the various parameters of diffusion tensor imaging (DTI) and CSF flow study of the cervical spinal cord using magnetic resonance (MR) imaging techniques. Materials and Methods: Intramedullary FA and MD were measured in the gray matter and posterior cord of the white matter and both lateral cords of the white matter at the C2-3, C4-5, C5-6 spinal levels. For the CSF flow study, velocity encoding was obtained at the C2-3, C4-5, C5-6 spinal levels. Results: There was a significant difference of the FA and MD between the white matter and gray matter (p < 0.05). The FA of the gray matter was significantly different according to the cervical spinal cord levels (p < 0.05). Otherwise, the FA and MD parameters were not significantly different (p > 0.05). The mean peak systolic velocity and mean peak diastolic velocity were $5.18{\pm}2.00cm/sec$ and $-7.32{\pm}3.18cm/sec$, respectively from C2 to C6 spinal cords. There was no significant difference in these velocities among the cervical spinal cord (p > 0.05). Conclusion: This basic information about DTI and CSF dynamics of the cervical spinal cord may be useful for assessing cervical spinal cord abnormalities using MR imaging.