• Journal of Environmental Science International
• /
• v.21 no.2
• /
• pp.253-261
• /
• 2012

We isolated and characterized novel duck feather-degrading bacteria producing keratinase. Twelve strains were isolated from soil and faces at poultry farm, and decayed feathers. They were identified as Bacillus methylotrophicus, Pseudomonas geniculata, Pseudomonas hibiscicola, Exiquobacterium profundum, Bacillus pumilus, Bacillus amyloliquefaciens, Chryseobacterium indologenes, Bacillus thuringiensis, Thermomonas koreensis, respectively, by phenotypic characters and 16S rRNA gene analysis. Generally, the level of keratinase production was not proportional to feather degradation rate. The highest keratinolytic activity was observed in the culture inoculated with Chryseobacterium indologenes D27. Although all strains did not degrade human hair, strains tested effectively degraded chicken feather(53.8-91.4%), wool(40.4-93.0%) and human nail (51.0-82.9%). These results suggest that strains isolated could be not only used to improve the nutritional value of recalcitrant feather waste but also is a potential candidate for biotechnological processes of keratin hydrolysis.

• Journal of Forest and Environmental Science
• /
• v.30 no.1
• /
• pp.76-84
• /
• 2014

This study was performed to monitor the effects of water level change on changes of landscape, vegetation community, and species diversity of riparian forest. Hantan river dam, study area, has been constructed in the area of Chansoo-myeon, Pocheon-si and Yeoncheon-eup, Yeoncheon-gun, Gyeonggi-do, which is a dam for flood control only in flooding season. Landscape changes were notable after the construction of coffer dam, and the changes were caused by water level increase in areas of riparian forests which consisted of mainly withered willow as a dominant species in the flooding season. It changed vegetation communities of riparian forest from Phragmites japonica and Salix koreensis to Phragmites japonica. Species diversity index was lowest in 2010 when the coffer dam was constructed and showed an increasing trend later. Thus, this study is well in agreement with a previous report that plants of the genus Salix wither by muddy water during flooding and also suggests, controlling water level of river and prediction of water level change's effects should be considered when any facilities are planned.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.1
• /
• pp.105-109
• /
• 2004

This study was conducted to investigate the potential origin of Weissella species, which were found in ingredients of kimchi, such as salted Chinese cabbage, radish, green onion, red pepper powder, pickled shrimps, garlic, and ginger. Ten strains of Weissella species (Weissella thailandensis, W. kimchii, W. koreensis, W. minor, W. halotolerans, W. hellenica, W. kandleri, W. confusa, W. viridescens, and W. paramesenteroides) and lactic acid bacteria isolated from ingredients of kimchi were analyzed by SDS-PAGE of whole-cell proteins. Several strains with patterns identical to those of Weissella kimchii were isolated from green onion. On the basis of biochemical characteristics and 16S rDNA sequence comparisons, these strains were identified as Weissella kimchii, suggesting green onion as a major origin of Weissella kimchii found in kimchi.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.7
• /
• pp.1300-1305
• /
• 2017

A rod shaped, aerobic, Gram-stain-negative, and motile bacterium, strain $AR2^T$, was isolated from a water sample of Yeongsan river, Republic of Korea. Strain $AR2^T$ clustered closely with the members of the genus Chitinimonas and showed the highest 16S rRNA gene sequence similarity with Chitinimonas prasina $LY03^T$ (96.4%), Chitinimonas viridis $HMD2169^T$ (96.4%), Chitinimonas taiwanensis $cf^T$ (96.2%), and Chitinimonas koreensis $R2A43-10^T$ (94.2%). The predominant fatty acids of strain $AR2^T$ were identified to be summed feature 3 (comprising $C_{16:1}{\omega}7c$ and/or $C_{16:1}{\omega}6c$), $C_{16:0}$, and $C_{10:0}3-OH$. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were found to be the major polar lipids. The genomic DNA G+C content was 60.4 mol%. Based on the polyphasic characterization, the isolated strain $AR2^T$ is described as a representative of a novel species in the genus Chitinimonas, for which the name Chitinimonas naiadis sp. nov. (type strain =$AR2^T$ =KCTC $42755^T$ =JCM $31504^T$) is proposed.

• Korean Journal of Poultry Science
• /
• v.40 no.1
• /
• pp.57-65
• /
• 2013

This study was conducted to evaluate the effect of dietary Weissella koreensis (Wk), a prominent kimchi lactic acid bacteria supplementation on growth performance, nutrients utilization, gut microbiota and meat characteristics in broiler chicken. Both live and killed Wk was compared to know which could be more efficacious as a feed probiotics. Three Wk supplemented groups and no Wk supplemented group were designated according to supplementation levels and cell status. Those were; Control (no Wk), 0.1 % live Wk (LWk 0.1), 0.5% live Wk (LWk 0.5) and 0.5% killed Wk (KWk 0.5). Body weight gain and feed conversion efficiency were improved (P<0.05) by dietary LWk supplementation. KWk did not exert any benefit on growth performance. Crude protein utilizability of KWk supplemented diet was lower (P<0.05) than that of other diets. However, there were no differences among treatments in other nutrients utilization. Serum IgG concentration and relative weight of bursa of Fabricius was highest (P<0.05) in broiler chicken fed KWk 0.5 diet. Cecal anaerobic lactic acid bacteria count of LWk groups were higher (P<0.05) than those of control and KWk 0.5 groups. Dietary Wk supplementation failed to lower the count of cecal and fecal E. coli. There was no effect of dietary Wk on TBARS values and fatty acids profile of broiler leg meat. However, the dietary supplementation of Wk exerted characteristic difference on electronic nose flavor of broiler meat. This study showed that dietary supplementation of LWk was able to improve body weight gain, feed conversion efficiency and cecal lactic acid bacterial count in broiler chicken. Further, the result of this study implemented that a live kimchi lactic acid bacteria, LWk, but not killed Wk, could be used as a probiotic feed supplement for broiler.

• Korean Journal of Ichthyology
• /
• v.11 no.1
• /
• pp.1-11
• /
• 1999

The five species of the genus Microphysogobio in Korea are reviewed with the keys to species and their illustrations: M. longidorsalis, M. jeoni sp. nov., M. koreeneis, M. yaluensis, and M. rapidus. Microphysogobio tungtingensis uchidai was treated as a junior synonym of M. yaluensis based on having the distinct papillae of upper lip and the color pattern on body sides by the examination of the type specimens. Microphysogobio jeoni is described as a new species from the specimens collected in the Naktong River, Han River and Keum River, Korea. it is well distinguished from the related species by the slender body, undeveloped papillae of upper lip and unspotted fins. Microphysogobio yaluensis was showed the clinal variations in the number of lateral line scales and vertebrae among the populations in the western drainages of Korea. It is remarked biogeographically that the five species of Microphysogobio are all endemics to Korea with the restricted ranges.

• Korean Journal of Ichthyology
• /
• v.17 no.2
• /
• pp.131-141
• /
• 2005

The ichthyofauna and structure of the fish community of Hapcheon Lake on the Hwang River were surveyed at six localities from May 2004 to Feb. 2005. There were 36 species belonging to 31 genera and 12 families in the Hapcheon Lake system. Of these, 24 species (66.7%) were cyprinids; cobitids and centropomids were next with 2 species (5.6%) each. The dominant species in the Hapcheon Lake system was Hemiculter eigenmanni (relative abundance 28.4%), the subdominant species Hemibarbus labeo (24.1%). Ten species (28.3%) of the 36 were Korean endemic species. Among the endemic species, H. eigenmanni and Squalidus chankaensis tsuchigae were abundant at each locality except site 6, but the eight other species were rare. In biomass, H. labeo comprised about 36.5% of the total weight, H. eigenmanni 22.3%, Cyprinus carpio 7.5%, Hypomesus nipponensis 6.9%, Culter brevicauda 3.7% and Lepomis macrochirus 3.6%. Average dominance, diversity, and evenness index of the fish community collected from six localities in the Hapcheon Lake system were 0.21, 0.85, and 0.67, respectively.

• Korean Journal of Environment and Ecology
• /
• v.27 no.3
• /
• pp.381-395
• /
• 2013

In this study, we surveyed 6 times for the actual vegetation type and flora of Amgok wetland (Intermediate moor area, Alnus japonica community area) and it's watershed area in Tohamsan district, Gyeongju National Park from Apr. to Nov. in 2011. As a result of the actual vegetation type, most vegetation of watershed area was disturbed by human activity except wetland area. Even though the wetland is relatively broad compared with it's watershed, the wetland: watershed is around 1:10. The flora was surveyed 65 family, 184 species, 1 subspecies, 39 variety, 8 forma and totally 233 taxonomic group in the watershed. The wetland type was looked intermediate moor. Naturalized plants was surveyed 2taxonomic in the wetland area and total was17 taxonomic. This area has relatively broad wetland and soundly conserved. But several Salix koreensis and Acer ginnala are growing so needed the interest and monitoring.

• Korean Journal of Ecology and Environment
• /
• v.35 no.3 s.99
• /
• pp.220-231
• /
• 2002

The fish fauna and Community were surveyed from April 2000 to September 2001 at 23 stations in the nam river, the first tributary located to the lower reach of the Nakdong River system. During the study period, 39 species belonging to 29 genera and 11 families were collected. Of which 14 species are endemic species or subspecies in Korea. Introduced species from other river system, O. bidens had not been collected at all after the report of Yang (1973) but many individuals were captured at main stream and tributary in this survey. Introduced species from abroad M. salmoides increased at most of stations. Dominant species in main stream were Z. platypus, O. bidens and H. labeo. On the contrary, Z. temmincki, Z. platypus and S. gracilis majimae dominated the tributaries. Diversity indices of main stream and tributaries were 0,979 and 0.966 respectively. St. 3 of main stream and St.1 and 16 of tributaries had high diversity indices (1.05, 0.962, 0.956) and lower dominant indices (0.102, 0.369, 0.200). As a whole, dominant index was 0.160, diversity index 1.044 and evenness index 0.656. It indicated that the community structure of this river was stable and diverse relatively.

• Korean Journal of Microbiology
• /
• v.47 no.1
• /
• pp.66-73
• /
• 2011

Bacterial density distributions of gut microbes in the digestive organs of Harmonia axyridis collected from three different sources (JK, CK, and CJ) were $6.0{\times}10^4$ CFU/gut under aerobic culture condition and $8.0{\times}10^6$ CFU/gut under anaerobic culture condition. Seven colony types were observed under aerobic condition and three types of similarity were detected under anaerobic condition. In total, 116 strains, including 34 strains under aerobic condition, were isolated from the digestive organs of H. axyridis. Based on the analysis of the 16S rRNA gene sequence, aerobic gut microbes were assigned to the Proteobacteria, Actinobacteria, Firmicutes, and Deinococcus-Thermus. A large number of isolates belonged to the genus Bacillus and Staphylococcus of the Firmicutes commonly found in H. axyridis from different sites. Anaerobic gut microbes were found to be similar according to colony morphological, phylogenetic analysis using ARDRA. Eighty-two anaerobic gut microbes were clustered into 17 different ARDRA types according to HaeIII. Representative anaerobic gut microbes in each ARDRA group were divided into five species of ${\gamma}$-Proteobacteria based on 16S rRNA gene sequence analysis; Hafnia alvei, Enterobacter ludwigii, Enterobacter kobei, Pseudomonas oryzihabitans and Pseudomonas koreensis. Phylogenetic analysis indicated that about 70% of the isolates belonged to ${\gamma}$-Proteobacteria, suggesting predominance of gut microbes.