• Journal of the Korean Wood Science and Technology
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• v.30 no.3
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• pp.42-47
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• 2002

This study was carried out to investigate the constituents of Sophora japonica(Leguminosae) bark. To isolate compounds, bark was extracted with ethanol and then partitioned with hexane, dichloromethane, ethylacetate and butanol successively. After partitioned, DCM fraction was subjected to column chromatography with various solvent system in silica gel and/or Sephadex LH-20. Structures were elucidated by spectroscopic methods including MS, ¹H, ¹³C and 2D-NMR experiments. Four compounds were isolated from the bark and identified as 3',7-dihydroxy-4'-methoxyisoflavone (cyclosin), puerol A, maackiain-3-O-𝛽-D-glucopyranoside (trifolirhizin), and 4', 5, 7-trihydroxyisoflavone (genistein). Among these compounds, cyclosin and trifolirhzin were first isolated from S. japonica.

• Korean Journal of Pharmacognosy
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• v.53 no.1
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• pp.1-7
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• 2022

In the previous study, we reported that luteolin isolated from Lonicera japonica methanolic extract had potent neuroprotective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of luteolin in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of luteolin. We used HT22 cell death injured by glutamate as a bioassay system. Luteolin decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca²⁺ concentration was decreased by luteolin treatment. Luteolin made mitochondrial membrane potential maintain to normal condition. It also increased not only glutathione reductase but also peroxidase to the control level. And it increased amount of glutathione, an endogenous antioxidant. These results suggested that luteolin isolated from L. japonica showed potent neuroprotective activity through the anti-oxidative pathway.

• Journal of Korean Society of Forest Science
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• v.95 no.5
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• pp.614-620
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• 2006

To study the chlorophyll contents and photosynthetic characteristics of 4 tree species of deciduous hardwoods; Betula platyphylla var. japonica, Zelkova serrata, Acer mono and Prunus sargentii were treated in 3 stages of shading; the full sun treatment, the medium shade treatment with 30% of transmittance comparing to full sun, the intense shade treatment with 8% of transmittance and their changes in chlorophyll contents and photosynthetic characteristics were examined and analyzed. Most hardwoods showed differences in the total chlorophyll contents in the order of May < September < July, however, that in Prunus sargentii increased progressively along with the lapse of time. Concerning the degree of shading, total chlorophyll contents increased in proportion to the level of shading. Betula platyphylla var. japonica and Prunus sargentii showed more than 2-3 times difference between the full sun treatment and the intense shade treatment. The changes in photosynthetic characteristics, the range of the light saturation point of the trees was $1,000{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in May, before the shading was applied, and the intensity was shown in the order of Betula platyphylla var. japonica > Zelkova serrata > Acer mono > Prunus sargentii. The photosynthetic rate was $6.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}{\sim}27.1{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of Betula platyphylla var. japonica > Prunus sargentii > Acer mono > Zelkova serrata that there were differences between species. Concerning the changes in light saturation point in each growth period after shading treatment, the light saturation point in the full sun treatment was found in the range of $560{\sim}1,100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and the level of intensity was shown in the order of May > July > September. The light saturation point decreased as the level of shading intensified and the level of changes in light compensation point in the full sun treatment for Betula platyphylla var. japonica and Prunus sargentii was shown in the range of $2.9{\sim}27.1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of May > July > September, however, for Zelkova serrata and Acer mono was shown in the range of $3.9{\sim}11.7{\mu}mol{\cdot}CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the order of July > May > September that there were differences between species.

• Journal of Wetlands Research
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• v.7 no.3
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• pp.87-95
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• 2005

Vegetation structure of the vascular plants was investigated from March 2003 to October 2003 in Haepyeong wetland, Gumi-si, Gyeongsangbuk-do, Korea. Actual vegetation of Haepyeong wetland largely can be classified by floristic composition and physiognomy into 18 communities; Xanthium strumarium-Digitaria sanguinalis, Humulus japonicus, Persicaria perfoliata-Humulus japonicus, Phragmites japonica-Miscanthus sacchariflorus, Persicaria hydropiper-Phragmites communis, Persicaria hydropiper, Phragmites japonica-Persicaria hydropiper, Miscanthus sacchariflorus- Phragmites japonica, Persicaria hydropiper-Phragmites japonica, Miscanthus sacchariflorus-Salix glandulosa, Salix nipponica-Salix glandulosa, Salix nipponica-Salix koreensis, Salix nipponica, Miscanthus sacchariflorus-Salix nipponica, Phalaris arundinacea-Salix nipponica, Salix glandulosa-Salix nipponica, Trapa japonica, and Ceratophyllum demersum-Trapa japonica. Among them, the area of the Salix nipponica-Salix koreensis community was the largest as 122.2ha(9.23%). The dominant vegetation type was Miscanthus sacchariflorus-Persicaria hydropiper community based on phytosociological method, and it was was classified into three subcommunities; Salix glandulosa-Salix nipponica subcommunity, Digitaria sanguinalis subcommunity, and Cyperus amuricus subcommunity. Differential species of Salix glandulosa-Salix nipponica subcommunity were Salix nipponica, S. glandulosa, S. koreensis, Scirpus radicans, Persicaria maackiana, and Achyranthes japonica; differential species of Digitaria sanguinalis subcommunity were D. sanguinalis, Setaria viridis, Ambrosia artemisiifolia var. elatior, and Cyperus orthostachyus; differential species of Xanthium strumarium subcommunity were X. strumarium, Acalypha australis, Erigeron canadensis, Echinochloa crus-galli, and Vicia tetrasperma. Zonation of vascular hydrophytes and hygrophytes was as followers: Salix glandulosa, S. koreensis, S. nipponica were distributed in the region of land which water table is low, and Persicaria maackiana, Persicaria hydropiper, Scirpus radicans were distributed in the understory. And emergent plants such as Phragmites communis and Scirpus karuizawensis, floating-leaved plant such as Trapa japonica, submersed plant such as Ceratophyllum demersum, and free floating plant such as Spirodela polyrhiza formed the zonation from shoreline to water. The specified wild plants designated by the Korean Association for Conservation of Nature, Ministry of Forest, and Ministry of Environment were not distributed in the study area. It was expected that Haepyeong Wetland worthy of conservation contributed purifying water pollution, giving habitats of many lifes, and providing beautiful scenes of the river.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.220-220
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• 2022

Disproportionating Enzyme 1 (DPE1) is an a-1,4-D-glucanotransferase that cleavages the a-1,4-glucosidic bonds and transfers glucosyl groups. In rice endosperm, it participates in starch synthesis by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Here, we investigated the haplotype variations and evolutionary indices (e.g., genetic diversity and population structure) for the DPE1 gene in 374 rice accessions representing seven subgroups (wild, indica, temperate japonica, tropical japonica, aus, aromatic, and admixture). Variant calling analysis of DPE1 coding regions leads to the identification of six functional haplotypes representing/occupying 8 nonsynonymous SNPs. Nucleotide diversity analysis revealed the highest pi-value in wild group (0.0556) compared to other cultivated groups, of which temperate japonica showed the most reduction of genetic diversity value (0.003). A significant positive Tajima's D value (1.6330) of admixture highlights sudden population contraction under balancing selection, while temperate japonica with the lowest Tajima's D value (-1.3523) showed a selection signature of DPE1 domestication which might be the cause of excess of rare alleles. Moreover, these two subpopulations exhibits a greater differentiation (F_ST=0.0148), indicating a higher genetic diversity. Our findings on functional DPE1 haplotypes will be useful in future breeding programs, and the evolutionary indices can also be applicable in functional studies of the DPE1 gene.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.216-216
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• 2022

The starch-branching enzymes (BEs) are responsible for synthesizing the amylopectin, which plays an important role in determining the structural and physical properties of starch granules. BE has two differently functioning isoforms (BEI and BEIIa/b) based on their difference in the chain-length pattern by the degree of polymerization (DP), which mainly contributes to the amylopectin chain length distribution in starch biosynthesis. In this study, we investigated functional haplotypes and evolutionary analyses of SBE1 in 374 rice accessions (320 Korean bred and 54 wild). The analyses were performed based on the classified subpopulations. Haplotype analysis generates a total of 8 haplotypes, of which only four haplotypes were functional carrying four functional SNPs in four different exons of SBE1 on chromosome 6. Nucleotide diversity analysis showed a highest pi-value in aromatic group (0.0029), while the lowest diversity value was in temperate japonica (0.0002), indicating the signal of this gene evolution origin. Different directional selections could be estimated by negative Tajima's D value of temperate japonica (-1.1285) and positive Tajima's D value of tropical japonica (0.9456), where the selective sweeps were undergone by both positive purifying and balancing selections. Phylogenetic analysis indicates a closer relationship of the wild with most of the cultivated subgroups indicating a common ancestor for SBE1 gene. FST-values indicate distant genetic relationships of temperate japonica from all other classified groups. PCA and population structure analysis show an admixed structure of wild and cultivated subpopulations in some proportions.

• Korean Journal of Pharmacognosy
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• v.12 no.1
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• pp.23-25
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• 1981

Methanol extracts of the roots of Atractylodes japonica (alba) (Compositae) and Gentiana scabra (Gentianaceae) were further fractionated with n-butanol, chloroform, n-hexane and water. Then each fraction was p.o. administered to an animal model of hepatitis caused by $CCl_4$ intoxication. Measurements of sleeping time of mice induced by hexobarbital administration indicated that n-butanol and chloroform fractions of Atractylodes japonica (alba), and chloroform and n-hexane fractions of Gentiana scabra showed significant liver protective activities. Measurements of the activities of serum transaminase, S-GOT and S-GPT appeared to support these results.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.1019-1021
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• 2009

Antiproliferative activity of the ethanol extract of Atractylodes japonica rhizomes (AJEX) was investigated using methyl thiazolyl tetrazolium (MTT) assays with various cancer cell lines (HL-60, MCF-7, SK-Br-3, MDA-MB-453, HepG2, Hep3B, PC-3, LNCaP, MKN 28, MKN 45, and HT-29 cells). Gastric carcinoma cell lines were the most responsive in terms of cell proliferation. The $IC_{50}$ of MKN 28 and MKN 45 cells were 35.98 and 27.57 ${\mu}g/mL$, respectively. Moreover, gastric carcinoma cells exposed to AJEX underwent apoptosis, as determined by Annexin V binding assay. Compared to respective control level, exposure to the AJEX at each $IC_{50}$ concentration resulted in a remarkable increase in the shift of cell populations. Present results suggest that AJEX possess potential anticancer properties.

• The Korean Journal of Mycology
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• v.26 no.2 s.85
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• pp.200-210
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• 1998

To study the species diversity of the genera Strobilomyces and Heimiella in Korea, 52 specimens were collected from 1982 to 1996 at 25 locations including Odaesan. Collected specimens were observed and three species among them were identified and described by observing macroscopic and microscopic characters. The three species are as follows; Strobilomyces floccopus (Vahl ex Fries) Karsten, S. confusus Singer, Heimiella japonica Hongo.

• The Korean Journal of Malacology
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• v.24 no.2
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• pp.105-107
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• 2008

Two trochid shells of the genus Ginebis, G. crumpii(Pilsbry, 1893) and the genus Enida, E. japonica A. Adams, 1860, collected from the coast of Jeju Island in Korea are reported. Ginebis crumpii and Enida japonica are closely related to Calliostoma Swainson, but can be differentiated by the features of the aperture and umbilicus. These species are a new records to the Korean Molluscan fauna. At present, G. crumpii and E. japonica are the only species of the represented in Korean water. Including the new records in this study, the family Trichidae contains 30 genera and 57 species in Korean water.