• Title/Summary/Keyword: S-plus

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Effect of Culture Medium and Additive on the Development of Bovine IVM/IVF Embryos (체외배양액과 첨가물질이 소 체외수정란의 체외발육에 미치는 효과)

  • 박동헌;황환섭;정희태;박춘근;김정익;김종복;양부근
    • Korean Journal of Animal Reproduction
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    • v.20 no.2
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    • pp.191-196
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    • 1996
  • The objective of this study were to investigate the effects of culture media and additives on the development of bovine in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. In experiment 1, bovine oocytes were cultured in droplets of TC 199 supplemented with 10% fetal bovine serum(FBS) with or without hormones (5$\mu\textrm{g}$/ml FSH, 5$\mu\textrm{g}$/ml LH, 1$\mu\textrm{g}$/ml E2). Cleavage rates of embryos cultured for 40~44hrs after IVF were higher when embryos were cultured in TC 199 supplemented hormones (68.1%, 921/35) than without hormones (52.7%, 77/146), but the percentages of development beyond morulae stage were not difference (20.7%, 19.4%). In experiment 2, the effects of various media such as TC 199, synthetic oviduct fluid(SOF), CR1aa with different energy source (fatal bovine serum, FBS; bovine serum albumin, BSA) on developmental capacity of IVM/IVF bovine embryos were investigated. The developmental rates into morulae and blastocysts were 27.1, 10.7, 6.3 and 0%, respecitvely, in CR1aa plus 3mg/ml BSA, SOF plus 10% FBS, TC 199 plus 10% FBS, SOF plus 3mg/ml BSA. In experiment 3, the comparisons of bovine embryos developed to morulae and blastocysts in different culture media (TC 199, SOF, CR1aa, Menezo's B2) were investigated. The developmental capacity beyond morulae stage were 32.9, 26.6, 11.1 and 7.1%, respectively, in Menezo's B2 plus BSA, CR1aa plus BSA, SOF plus BSA, TC 199 plus FBS medium. The cell numbers of the blastocyst were not different in different cultrue media. In experiment 4, bovine embryos were co-cultured with vobine oviduct epithelial cells(BOEC) in TC 199 plus FBS, SOF plus BSA, CR1aa plus BSA, Menezo's B2 plus BSA. The morula and blastocyst rates were 44.7, 32.9, 26.0 and 23.3%, respectively, in CR1aa TC 199, SOF, and Menezo's B2 medium. The cell numbers of the blastocyst were similar to those of blastocyst developed in different culture media.

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Introduction to S-PLUS and graphical comparison with SAS (S-PLUS의 소개 및 SAS 와의 그래픽 비교)

  • 김성수;한경수
    • The Korean Journal of Applied Statistics
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    • v.6 no.1
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    • pp.1-11
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    • 1993
  • Statistical graphics have been important new tools for data analysis and many statistical softwares are exploiting graphical methods. Among these softwares available in personal computer at low cost, we intriduce S-PLUS(version 2.0). S-PLUS is an interactive graphical data analysis system and object-oriented programming language. SAS/GRAPH is another popular graphical system for displaying data in the form of color plots, charts, maps, and slides on screen and hardcopy devices. S-PLUS is compared to SAS/GRAPH(version 6.04) in viewpoints of statistical graphics.

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Methods for environmental risk assessment of rice transgenic plants expressing small non-coding RNA (Small non-coding RNA를 발현하는 형질전환 벼의 환경위해성 평가 방법)

  • Jin, Byung Jun;Chun, Hyun Jin;Cho, Hyun Min;Lee, Su Hyeon;Choi, Cheol Woo;Jung, Wook-Hun;Baek, Dongwon;Han, Chang-deok;Kim, Min Chul
    • Journal of Plant Biotechnology
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    • v.46 no.3
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    • pp.205-216
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    • 2019
  • Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.

Effects of a Mixture Ginseng Radix, Chaenomelis Fructus on the Mice Model of Alzheimer's Disease (인삼(人蔘), 목과(木瓜) 추출액이 Alzheimer성 치매의 병태(病態) 모델에 미치는 영향)

  • Han, Sin-Hee;Kil, Gi-Jung
    • The Korea Journal of Herbology
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    • v.22 no.1
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    • pp.41-48
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    • 2007
  • Objectives : This research was investigated the effect of the Ginseng Radix plus Chaenomelis Fructus on Alzheimer's disease. Methods : Specifically, the effects of the Ginseng Radix plus Chaenomelis Fructus extract on $IL-1{\beta}$, $TNF-{\alpha}$ of BV2 microglia cell line treated with lipopolysacchride. Results : The Ginseng Radix plus Chaenomclis Fructus extract suppressed the over-expression of $IL-1{\beta}$ protein, $TNF-{\alpha}$ protein, MDA, and CD68/CD11b, in the mice with Alzheimer's disease induced by ${\beta}$ amyloid peptide. Conclusion: These results suggest that the Ginseng Radix plus Chaenomelis Fructus extract may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the Ginseng Radix plus Chaenomelis Fructus extract for Alzheimer's disease is suggested for future research.

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A Study on the Therapeutic Effect of Alzheimer's Disease of Ginseng Radix plus Crataegi Fructus. (인삼산사복합방이 Alzheimer성 치매의 치료 효과에 대한 연구)

  • Han, Sin-Hee;Kil, Gi-Jung
    • The Korea Journal of Herbology
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    • v.22 no.1
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    • pp.35-40
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    • 2007
  • Objectives : This in vitro research was conducted to investigate the effect of the Ginseng Radix plus Crataegi Fructus. on the cytokine protein release and Nitric oxide release in releted to Alzheimer's disease. Methods : Specifically, the effects of the Ginseng Radix plus Crataegi Fructus extract on $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ of BV2 microglia cell line treated with lipopolysacchride. Results: The Ginseng Radix plus Crataegi Fructus extract suppressed the production of inflammatory cytokine protein $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ and in BV2 microglia cell line treated with lipopolysacchride. Conclusion: These results suggest that the Ginseng Radix plus Crataegi Fructus extract may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the Gin-CHF extract for Alzheimer's disease is suggested for future research.

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A Comparison of Multivariate R-Techniques in SAS, SPSS, Minitab and S-plus (SAS, SPSS, MINITAB, 5-PLUS에서 다변량 R-기법의 비교)

  • 최용석;문희정
    • The Korean Journal of Applied Statistics
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    • v.17 no.1
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    • pp.153-164
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    • 2004
  • In this study, we compare multivariate R-techniques in the up-to-date versions of SAS, SPSS, Minitab and S-plus. The direct input method by typing in command is considered for SAS, while the menu-driven method is considered for SPSS, Minitab and S-plus. Comparison was made in terms of input data format, input option, charts and outputs.

Body-shape characteristics and body types of plus-size men in their 30s and 40s based on Korean anthropometric data (사이즈 코리아 인체 측정 자료에 근거한 30~40대 플러스 사이즈 남성의 체형 특성 및 체형)

  • Lee, Hana
    • The Research Journal of the Costume Culture
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    • v.28 no.5
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    • pp.639-651
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    • 2020
  • This study utilized data to classify and characterize the body types of plus-size adult men aged in their 30s and 40s. Diversity is an important factor in the era of inclusive design, and discussion about size diversity to include the plus size should be accommodated. Data from 493 adult men classified as obese (with a World Health Organization criterion ≥25 BMI) were used for the analysis. The results of the study are as follows. Six independent factors were extracted using factor analysis for cluster analysis, which were then classified into five types. Type 1 (29.01%) was identified as body type I with the smallest degree of obesity. Type 2 (15.4%) was identified as body type Y with wide shoulders and a thin waist. Type 3 (14.2%) was the largest body volume (body type O), while the fourth (19.27%) identified as body type H has a large height and upper body. Lastly, type 5 (22.11%) has a long lower body and a slim abdomen, referred to as body type X. This study presents a basis for the development of various clothing sizes utilizing the body shape characteristics of plus-size men in their 30s and 40s. Follow-up research is needed to develop patterns for plus size men and to design various products.

Micro-computed tomographic evaluation of single-cone obturation with three sealers

  • Sahar Zare;Ivy Shen;Qiang Zhu;Chul Ahn;Carolyn Primus;Takashi Komabayashi
    • Restorative Dentistry and Endodontics
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    • v.46 no.2
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    • pp.25.1-25.12
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    • 2021
  • Objectives: This study used micro-computed tomography (µCT) to compare voids and interfaces in single-cone obturation among AH Plus, EndoSequence BC, and prototype surface pre-reacted glass ionomer (S-PRG) sealers and to determine the percentage of sealer contact at the dentin and gutta-percha (GP) interfaces. Materials and Methods: Fifteen single-rooted human teeth were shaped using ProTaper NEXT size X5 rotary files using 2.5% NaOCl irrigation. Roots were obturated with a single-cone ProTaper NEXT GP point X5 with AH Plus, EndoSequence BC, or prototype S-PRG sealer (n = 5/group). Results: The volumes of GP, sealer, and voids were measured in the region of 0-2, 2-4, 4-6, and 6-8 mm from the apex, using image analysis of sagittal µCT scans. GP volume percentages were: AH Plus (75.5%), EndoSequence BC (87.3%), and prototype S-PRG (94.4%). Sealer volume percentages were less: AH Plus (14.3%), EndoSequence BC (6.8%), and prototype S-PRG (4.6%). Void percentages were AH Plus (10.1%), EndoSequence BC (5.9%), and prototype S-PRG (1.0%). Dentin-sealer contact ratios of AH Plus, EndoSequence BC, and prototype S-PRG groups were 82.4% ± 6.8%, 71.6% ± 25.3%, and 70.2% ± 9.4%, respectively. GP-sealer contact ratios of AH Plus, EndoSequence BC, and prototype S-PRG groups were 65.6% ± 29.1%, 80.7% ± 25.8%, and 87.0% ± 8.6%, respectively. Conclusions: Prototype S-PRG sealer created a low-void obturation, similar to EndoSequence BC sealer with similar dentin-sealer contact (> 70%) and GP-sealer contact (> 80%). Prototype S-PRG sealer presented comparable filling quality to EndoSequence BC sealer.

Effects of Ginseng Radix plus Crataegi Fructus on the Gene Expression in Relation to Alzheimer's Disease. (인삼산사복합방(人蔘山査複合方)이 Alzheimer성 치매와 관련된 유전자 발현(發顯)에 미치는 영향)

  • Han, Sin-Hee;Kil, Gi-Jung
    • The Korea Journal of Herbology
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    • v.21 no.4
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    • pp.133-141
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    • 2006
  • Objectives : This research was investigated the effect of the Ginseng Radix plus Crataegi Fructus on the gene expression in relation to Alzheimer's disease. Methods : Observed gene expression of the Ginseng Radix plus Crataegi Fructus extract on $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2, and NOS-II mRNA of BV2 microglia cell line treated with lipopolysacchride. Results : The Ginseng Radix plus Crataegi Fructus extract suppressed the gene expression of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2, NOS-II mRNA in BV2 microglia cell line treated with lipopolysacchride. Conclusion : These results suggest that the Ginseng Radix plus Crataegi Fructus extract may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the Ginseng Radix plus Crataegi Fructus extract for Alzheimer's disease is suggested for future research.

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