• Korean Journal of Weed Science
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• v.15 no.1
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• pp.19-29
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• 1995

The effect of seeding depth on the growth, mesocotyl elongation and herbicidal response of rice(Oryza sativa L., Japonica type, cv. "Tongjin") and barnyardgrass(Echinochloa crus-galli Beauv. var orizicola Ohwi.) were studied in greenhouse experiments. Barnyardgrass growth as affected by different water depths was briefly tested. Rice and barnyardgrass were broadcast in soil into Oem, 1cm, 2cm and 3cm in seeding depth under dry direct-seeded condition. Butachlor(N-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide) at dose rate of 1800g ai/ha and thiobencarb(S-[(4-chlorophenyl)methyl] diethyl carbamothioate) at dose rate of 2100g and 4200g ai/ha were soil applied to them at 5 days after seeding. At 10 days after seeding, plants harvested to examine their growth as affected by seeding depth. Root length and shoot fresh weight of rice untreated was greatest in 1cm- and 3cm-seeding depth, respectively, however, mesocotyl did not elongate. While plant height of barnyardgrass grew regardless of seeding depth and water depth, but root length was greatest in 1cm-seeding depth of dry condition and reduced with increased water depth. And mesocotyl was elongated in only dry condition and its length increased with increased seeding depth. At 10 days after application thiobencarb applied pre-emergence inhibited plant height, and shoot fresh weight of rice in only 0cm-seeding depth under dry condition whereas was unaffected in above 1cm-seeding depth, similar to untreated control, and ever increased root length and root fresh weight of rice. On the other hand, shoot, root and leaf growth of barnyardgrass was, severely inhibited regardless of application rates and seeding depths. Reduction of shoot growth by treatment of herbicide was significantly greater than that of root growth. Consequently, reduction of barnyardgrass growth by treatment of thiobencarb did not be associated with seeding depth and mesocotyl elongation as affected by different seeding depth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.61-79
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• 2009

This study is for incompatible element contents of Perilla frutescens and Sesamum indicum from the Keumsan: biotite granite, phyllite and shale areas. In the soils, high elements are shown in the granite and phyllite areas, and in the areas of the Perilla frutescens. Positive correlations are distinctive within the granite for the Perilla frutescens, but the shale for the Sesamum indicum. These relationships can be explained with relative propositions of minerals containing the incompatible element. In the plants, high elements are shown in the shale and the Sesamum indicum are high in the comparisons of the same soil types. The low parts are mainly high. Regardless of the soil types, the lower and upper parts, respectively, are high in the Y, Zr and Rb contents for the Perilla frutescens, but, Ta, Nb, Th and U contents for the Sesamum indicum. Positive correlations are distinctive within plants of the phyllite. Comparing with the soil types, all HFS and Cs contents of the LFS in the plants are low with differences of several to hundred times, but high in the Sr contents with differences of several times. In the comparisons between plants and soil types, Y, Zr, Hf, Ta, Nb, Rb, and Sr of the phyllite and Th, U, Ba and Cs of the shale for the Perilla frutescens as well as Y, Zr, Hf, Rb, Sr, Ba and Cs of the phyllite and Ta, Nb, Th and U of the shale for the Sesamum indicum are chemically similar to the soils. In the comparisons of the each parts for the plant types, differences with the soils are big in the granite.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.3
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• pp.211-216
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• 2005

This study was conducted from March 21 to July 10 in 2004 at JeJu to investigate the influences of worm casting organic fertilizer rates (0, 150, 300, 450, 600 kg/10a) on creeping bentgrass sward. The result obtained were summarized as follows; plant height was getting longer as organic fertilizer increased from 0 to 600 kg/10a. But it was no significance from 450 to 600kg/10a. Root length, SPAD reading value, leave and root weight were the same trend with plant height response. Percentage of land cover and density of creeping bentgrass increased as fertilizer rate increased from 0 to 600 kg/10a. But percentage of land cover and density of weed decreased. Number of weed species were decreased as increasing of organic fertilizers. Then ranking of the dominant weeds were Chenopodium album var. cetrorubrum and Digitaria adscendens, Polygonum hydropiper (at 0kg/10a), Portulaca oleracea and Digitaria adscendens, Polygonum hydropiper (at 150kg/10a), Polygonum hydropiper and Poa annua, Digitaria adscendens (at 450 kg/10a), Polygonum hydropipr and Digitaria adscendens, Portulaca oleracea (at 600 kg/10a).

• Korean Journal of Ecology and Environment
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• v.44 no.2
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• pp.239-251
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• 2011

To compare the different water environments in the Jigsaw stream (st. 1, 2) and Jujin stream (st. 3, 4) crossing over the Byeonsan-Bando and Seonunsan parklands, the physico-chemical factors and the species compositions of epilithic diatoms were analyzed from March 2009 to February 2010. The mean values of conductivity (681.1 ${\mu}S\;cm^{-1}$), suspended solids (27.83 mg $L^{-1}$), biological oxygen demand (3.15 mg $L^{-1}$), total nitrogen (3.24 mg $L^{-1}$), total phosphorus (0.24 mg $L^{-1}$) and chlorophyll-${\alpha}$ (12.99 mg $m^{-3}$ ) in Jujin stream were significantly higher than each element in Jigsaw stream. Eighty taxa were classified into 73 species and seven varieties belonging to 24 genera, eight families, three suborders and two orders in Jigsaw stream. Eighty eight taxa were classified into 81 species and seven varieties belonging to 22 genera, seven families, three suborders and two orders in Jujin stream. Biological water quality assessments revealed that Jigsaw stream was ${\beta}$~${\alpha}$-oligosaprobic and Jujin stream was ${\beta}$-mesosaprobic. Dominant and diversity indices were indicated from 0.25~0.81 and from 1.73~4.14 in Jigsaw stream, respectively, and from 0.29~0.64 and from 2.72~4.02 in Jujin stream, respectively. Jujin stream was more eutrophic than those of Jigsaw stream. The different water environments between the two streams could be due to the different ecosystems of the neighboring basins of each stream crossing over in the mountain. Further studies should investigate the water environments of two streams by continuous and regular monitoring to analyze the different ecosystem mechanisms.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.61-62
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• 2003

Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.62-70
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• 2003

The biological activities on human immune and cancer cell lines of the four kinds of Korean mistletoes (Korean Viscum album, var. coloratum, : Korean Viscum sp. in Quercus acutissima Carr., Korean Viscum sp. in Castanea crenata, Korean Viscum sp. in Betula platyphylla, and Korean Viscum sp. in Salix koreensis) extracts were investigated. The extracts were preparated with ethanol, and fractionated with n-butanol, ethyl acetate, chloroform, hexane, and second distilled water. Cytotoxic potencies of the fractions on human normal lung cell line (HEL 299) showed under 28% in the concentration of 0.5 mg/ml. Growth inhibition effect of the Korean mistletoe extracts on the several human cancer cell lines depends on the concentration of the extracts, and extracting solvent. The hexane, chloroform, and ethyl acetate fractions indicated a strong anticancer activity, but not in aqueous and butanol fractions. Some mistletoe fractions have a different characteristic on the cancer cell lines. Stimulation on the growth of human immuno cell lines(B cell : Raji, T cell: Jurkat) of the extracts were confirmed in the ethyl acetate, chloroform, hexane fractions, but not in aqueous system.

• Applied Microscopy
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• v.15 no.2
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• pp.31-68
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• 1985

Authors are trying to unveil the ultrastructural organization of olfactory bulb, which has been summerized under light microscopic level or communicated only in some detail in different view point until now. For the critical point of view, since the phylogenetical approach will give the ultimate value in the correlative study between structural and functional bases (Brodal, 1969), the present study was carried out light and electron microscopic analyses of the structures of the neurons and synaptic organizations in olfactory bulbs from different animals in phylogenetical scale. We selected each one species from five animal classes: the house rabbit(Oryctolagus cuniculus var. domesticus [Gmelin]) from Mammalia, the domestic fowl (Gallus gallus domesticus Brisson) from Aves, the viper (Agkistrodon hylys [G.P. Pallas]) from Reptilia, a frog (Bombiana orientalis Boulenger) from Amphibia and the crussian carp (Carassius carassius [Linne]) from Pisces. For light microscopic study, samples were fixed in 10% formalin and paraffin sections were stained with hematoxylin-eosin. For the electron microscopic study, the tissues were fixed by perfusion through the heart or immersion with 1% paraform-aldehyde-glutaraldehyde mixture (phosphate buffer, pH 7.4), and final tissue block trimmed under dissecting microscope were osmicated (1% OsO4), they were embedded in Araldite or Epon 812, and ultrathin sections were made by LKB-V ultratome following the inspection of semi-thin sections stained with toluidine blue-borax solution. Ultra-thin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100CX electron microscope. We have summerized our morphological analyses as follows: 1. The olfactory bulb of rabbit, viper and frog shows the eight layers of fila olfactoria, glomerular, external granular, external plexiform, mitral cell, internal plexiform, internal granular, medullary but domestic fowl shows the five layers of glomerular, fibrillar, mitral, granular and medullary and the three layers of fibrilla, glomerular and medullary in crussian carp. The sharpness of demarcation between the layers shows deferential tendency according to phylogenetical order. 2. Mitral cells of vertebrate have large triangular or oval shape with spherical nuclei which contain not so much chromatin. The cytoplasm contains numerous cell organelles, of which Nissl's bodies or granular endoplasmic reticula arranged as parallel strands. Development of granular endoplasmic reticula were declined as the phylogentical grade is going lower. 3. Tufted cells of all animal are mostly spindle or polygonal contour and contain oval nuclei which located in periphery of cytoplasm. The nuclei of rabbit, fowl, viper and frog has relatively space chromatin, but a nucleus of crussian carp contain irregularly aggregated chromatin in karyoplasm. Their cytoplasmic volume and cell organelle contents are in between those of mitral cell and granular cell. They contain moderate amount of mitochondria, granular endoplasmic reticula, a few Golgi complex, polysomes, lysosome, etc. 4. Granule of cells of all the vertebrate amimals studied exhibit similar features; cells and their dense nuclei show spherical or oval contour, and they have the thin rim of cytoplasm which contain only a few cell organelles. 5. In rabbit, the soma of mitral cells were in contact with boutons with two types of synaptic vesicles, that is, round and flat vesicles, especially flat vesicles in boutons were showing reciprocal synapses. However, in domestic fowls, vipers, frogs and crussian carps, there were found boutons showing only spherical synaptic vesicles. 6. The boutons containing round synaptic vesicles were made contact with the some of tufted cell of olfactory bulb in the rabbits, fowls, vipers and frogs, but no synaptic boutons were observed in soma of tufted cells in crussian carps. In the frogs, there were observed dendrites were contact with the soma of tufted cells. 7. In the neuropils of plexiform, granular and glomerular layers olfactory bulbs in the vertebrate, the synapses were axo-large dendrites, axo-median and small dendrites, dendrodendritic, and axo-axonal contacts. However, in the neuropil of crussian carps, synapses were observed only in glomerular layer.

• Applied Microscopy
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• v.36 no.1
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• pp.35-45
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• 2006

Plants of Vitex negundo are known to develop numerous trichomes throughout their body, where certain trichome types have been believed to be one of the plausible structures for the unique scents. In the current study. structural aspects of the trichomes have been examined in leaves and stems of Vitex negundo using TEM and SEM. Trichome types as well as structural changes that occurred in certain trichomes during secretion have been mainly focused. Three type of glandular trichomes and two types of non-glandular trichomes were developed in the epidermis of young and mature Vitex negundo plants. The glandular trichomes included the peltate type (Type 1), the capitate type (Type 2), and degraded capitate type (Type 3), whereas the non-glandular warty trichomes contained the multicellular (Types 4) and unicellular type (Type 5). Type 1 and 2 consisted of head and stalk cells, but their number and size were different. One secretory cavity was formed from the four head cells in the former, but only two head cells were involved in the latter. The cytoplasmic density in the head cell was quite high and in particular, sER and Golgi bodies were well developed. At initiation of their development, the cuticle layer of the head cells separated from the outer tangential wall to form a secretory cavity. Subsequently the cavity expanded acropetally and a large number of secretory vesicles continuously produced from the head cells until they filled the entire cavity. The cavity contained materials that would be soon discharged into intercellular spaces and/or into the air. The cavity began to decrease the volume by contracting at initial secretion but degrade rapidly within short time. It has been suggested that the mode of secretion in V. negundo is probably the eccrine secretion, since no break or rupture of the cavity has been observed during examination. Contrastingly Type 3 exhibited deterioration of the head cell at early stage. Type 4 was about $110{\sim}190{\mu}m$ long, consisting of $2{\sim}3$ cells, and distributed more in the adaxial epidermis compared to the abaxial surface. However, $20{\sim}30{\mu}m$ long Type 5 was extremely dense in both epidermis. Among several trichome types, Type 1 and 2 probably play an important role in discharging unique aromatic scents in plants of V. negundo.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.179-183
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• 2000

Satsuma mandarin(Citrus unshiu Marc. var. miyagawa) was stored at $3^{\circ}C$ and 85% relative humidity, and then the changes of firmness, pectin- degrading enzymes activity and other physicochemical properties of citrus fruits during storage were investigated. Firmness of fruits with 2 m probe was decreased quickly from 1,176.8 g-force to 503.6 g-force, and moisture of peel and flesh were decreased from 75.3% to 74.9%, and from 91.8% to 90.7% during maturation, respectively. Decay ratio was increased to 18.75% after 90 days' storage, and after then it was increased rapidly. Weight loss was increased gradually to 24.5% during long-term storage. Firmness with 2 mm probe were decreased from 538.9 g-force to 336.9 g-force gradually during storage. Peel moisture was decreased from 75.8% to 72.6%, and flesh moisture was also decreased gradually from 90.3% to 88.3% during storage. Exopoly-galacturonase activity of peel and flesh were increased from 326.0 units/100 g to 534.9 units/100 g, and from 63.1 units/100 g to 81.0 units/100 g at 90 day's storage, respectively. After then, He enzyme activities were decreased from 394.0 units/100 g and 38.0 units/100 g, respectively. Pectinesterase activity of peel and flesh were increased from $14.4\;{\mu}mol$ to $38.8{\mu}mol$, and from $26.0{\mu}mol$ to $39.0{\mu}mol$ at 60 days' storage, respectively. After then, the enzyme activities were decreased to $6.0{\mu}mol$ and $8.2{\mu}mol$, respectively.

• Korean Journal of Heritage: History & Science
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• v.43 no.1
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• pp.190-211
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• 2010

The purpose of this paper is to provide the preliminary data for maintenance of old trees of natural monuments and establishing protection projects. Accordingly, growth conditions, location circumstances, environment and protection management were analyzed for old trees at 34 sites in Gyeongsangbuk-do. Tree forms, crown, trunk and trees' health, leaves volume were analyzed in survey sites. As the results, the growth of Picrasma quassioides in Songsadong, Andong-si and Catalpa ovata in Hongwon-ri, Cheongsong-gun were not in good condition, along with other old trees of 6 cities (Quercus variabilis in Susan-ri, Uljin-gun, Juniperus chinensis in Hujeong-ri, Uljin-gun, Pyrus ussuriensis in Ssangjeon-ri, Uljin-gun, Salix glandulosa var. pilosa in Deokchon-ri, Cheongdo-gun, Pinus densiflora for. multicaulis in Hwasan-ri, Mungyeong-si, Pinus densiflora in Daeha-ri, Mungyeong-si). Accoding to these results, protection project and consistent monitoring are necessary. The analysis results of growth conditions per location circumstances showed that the old trees in the small spaces with large traffic of people were in bad condition. Therefore, it is necessary to secure sufficient space and soil improvement for such trees. The analysis results of growth conditions per species showed that Zelkova serrata, Quercus spp., Pinus densiflora are in good condition while Salix koreensis and others, i.e. a singular tree, are in poor condition. Thus, relatively smaller individuals and separately situated trees need careful management in their protection. The analysis results of trunk's growth ratio showed that Pinus densiflora, Ginkgo biloba, Zelkova serrata are in good condition whereas Juniperus chinensis, Quercus variabilis, Salix koreensis are not. It is a recent trend that tree surgeries are gradually decreasing; there are a number of damaged trees with no maintenance/management, and several sites requiring mend trunks management and cabling, bracing in protection and management projects. Hence, in the future, local government and management organizations should plan for systematic management and establish a protection plan for the natural monument trees. Also, 12 sites were found to be in need to purchase lands in order to secure the growth spaces, and since most of these areas are available for purchase, it is needed to promote a budget and purchase those areas through persuasion of the owner. These areas seems crucial in order to maximize the effect of conservation efforts and budgets for the preservation and restoration of the old trees.