• Title/Summary/Keyword: S-Sugars

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UV-spectrophotometric and electrophoretic analysis composition of protein and the content of sugars of young Ricinus communis L. by cold treatment (UV-분광광도법과 전기영동에 의한 피마자 유식물에서 저온처리에 의한 당류 함량과 단백질 구성의 분석)

  • Cho, Bong-Heuy;Park, Sun Young;Park, Myon-Yong
    • Analytical Science and Technology
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    • v.8 no.2
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    • pp.181-186
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    • 1995
  • The contents of sucrose and reducing sugars of cotyledons and hypocotyls of Ricinus communis L were increased slightly during cold treatment at $4^{\circ}C$. The concentration of total amino acids was increased continuosly during cold treatment. But the contents of hydrophilic amino acids, Asp/Asn, Glu/Gln, Thr, Ser, Ala and cationic amino acids, Arg and Lys were varied dramatically with the cold treated time. The cold treatment induced 24, 52, 54, 55, 56 and 73.5kD of proteins in cotyledons and 55, 56 and 73.5kD of proteins in hypocotyls. 24, 42, 49 and 52kD of proteins in cotyledons and hypocotyls were boiling stable. They were not denatrated by boiling at $100^{\circ}C$.

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Hydrogen gas production by fermentation from various organic wastewater using Clostridium butyricum NCIB 9576 and Rhodopseudomonas sphaeroides E15-1 (각종 유기성 폐수로부터 Clostridium butyricum 및 Rhodopseudomonas sphaeroides에 의한 수소생산)

  • Yoon, Young-Sue;Kim, Hyun-Kyung;Ryu, Hye-Yeon;Lee, In-Gu;Kim, Mi-Sun
    • Transactions of the Korean hydrogen and new energy society
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    • v.11 no.1
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    • pp.29-41
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    • 2000
  • Anaerobic fermentation using Clostridium butyricum NCIB 9576, and photo-fermentation using Rhodopseudomonas sphaeroides E15-1 were studied for the production of hydrogen from Makkoli, fruits (orange & apple, watermelon & melon) and Tofu wastewaters. From the Makkoli wastewater, which contained $0.94g/{\ell}$ sugars and $2.74g/{\ell}$ soluble starch, approximately $49mM\;H_2/{\ell}$ wastewater was produced during the initial 18h of the anaerobic fermentation with pH control between 6.5-7.0. Several organic acids such as butyric acid, acetic acid, propionic acid, lactic acid and ethanol were also produced. From Watemlelon and melon wastewater, which contained $43g/{\ell}$ sugars, generated about approximately $71mM\;H_2/{\ell}$ wastewater was produced during the initial 24 h of the anaerobic fermentation. Tofu wastewater, pH 6.5, containing $12.6g/{\ell}$ soluble starch and $0.74g/{\ell}$ sugars, generated about $30mM\;H_2/{\ell}$ wastewater, along with some organic acids, during the initial 24 h of anaerobic fermentation. Makkoli and Tofu wastewaters as substrates for the photo-fermentation by Rhodopseudomonas sphaeroides E15-1 produced approximately 37.9 and $22.2{\mu}M\;H_2/m{\ell}$ wastewaters, respectively for 9 days of incubation under the average of 9,000-10,000 lux illumination at the surface of reactor using tungsten halogen lamps. Orange and apple wastewater, which contained 93.4 g/l, produced approximately $13.1{\mu}M\;H_2/m{\ell}$ wastewater only for 2 days of photo-fermentation and the growth of Rhodopseudomonas sphaeroides E15-1 and hydrogen production were stopped.

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Study on the Compositional Change of Free Sugars and Glucosinolates of Rapeseed during Germination (발아에 의한 유채의 Glucosinolate 및 유리당 함량의 변화에 관한 연구)

  • Kim, In-Sook;Kwon, Tae-Bong;Oh, Sung-Ki
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.194-199
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    • 1988
  • The objective of this study was to investigate the technical feasibility of producing toxicant-free rapeseed by germination. To this end, rapeseed(Brassica napus L.)was germinated at $25^{\circ}C$ for 120 hours, and the chemical compositions-glucosinolates and free sugers-were determinated in every 24 hours during germination. The amount of glucosinolates in rapeseed measured by UV method was very close to that measured by GLC method. The glucosinolates were considerably abundant in rapeseed before germination, and the total content was found to be 13.6 mg/g. Rapeseed showed the lowest glucosinolate content in 72 hours during germination, and it gradually increased glucosinolate content from 96 hours. Free suger content in rapeseed before germination was as follows : 3.03 mg/g of fructose, 2.97 mg/g of glucose and 5.63 mg/g of sucrose. Raffinose and stachyose were not detected, and in general free sugars were gradually decreased during germination. However, sucrose was increased in the early period of gremination and decreased in the later period.

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Ethanol Fermentation by Pichia Stipitis in a Mixture of Pentoses and Hexoses (오탄당과 육탄당의 혼합용액에서 Pichia stipitis에 의한 에탄올 발효)

  • 정봉환;유연우서진호
    • KSBB Journal
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    • v.9 no.4
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    • pp.395-399
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    • 1994
  • P. stipitis CBS5776 was cultivated to examine the characteristics of ethanol fermentation for hexoses (mannose, g1ucose, and galactose) and pentoses(xylose and arabinose). Glucose was the best carbon source among the sugars used in terms of ethanol yield. Glucose was used to produce ethanol with an yield coefficient 0.376g ethanol/g glucose, whereas mannose was converted to produce ethanol with an yield coefficient 0.326g ethanol/g mannose. P. stipitis CBS5776 was also grown in a mixture of sugars to study the pattern of carbon utilization. The yeast utilized glucose and mannose firsts and then galastose and xylose as carbon sources. Arabinose was partially used for biomass when it was present as a sole carbon source, but it was not metabolized at all in a mixture of carbon sources. P. stipitis produced $12.2g/\ell$ ethanol with a yield coefficient 0.332 g ethano1/g sugar in a mixture of sugars.

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Bioethanol Production from Seaweed Ulva pertusa for Environmental Application (해조류 구멍갈파래 (Ulva pertusa)의 친환경적 이용을 위한 바이오에탄올의 생산)

  • Kim, Jong-Deog;Yoon, Yang-Ho;Shin, Tai-Sun;Kim, Min-Yong;Byun, Hyun-Soo;Oh, Seok-Jin;Seo, Hyo-Jin
    • KSBB Journal
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    • v.26 no.4
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    • pp.317-322
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    • 2011
  • Ulva pertusa is one of the worst pollutant like a waste vinyl after agriculture and caused bad smell at seashore in Jejudo and south area of korean peninsular. For favorable environmental utilization of Ulva pertusa, it could be applied for ethanol production with its acid hydrolysate. The components of hydrolysate included fermentable sugar of glucose, xylose, mannose, galactose, and higher amounts of unfermentable rhamnose. Fermentable sugars were converted to ethanol with S. cerevisiae, also xylose to ethanol with P. stipitis, their maximun ethanol production at optimum conditions were 462 ${\mu}g$/mL and 475 ${\mu}g$/mL, respectively. While, rhamnose cannot be changed to ethanol with S. cerevisiae or P. stipitis, alone. Combination of S. cerevisiae and P. stipitis can convert rhamnose to ethanol, because P.stipitis degradaded rhamnose to pyruvate, and then S. cerevisiae convert to ethanol, at optimum conditions, ethanol reached to 782 ${\mu}g$/mL (30.24%) that is higher than that of 2 strain alone from 500 mg of dried Ulva pertusa contained 2586.45 ${\mu}g$/mL of reduced sugars. Ulva pertusa can be utilized for renewal energy insted of environmenatal enemy.

Effects of Salting Methods on the Physicochemical Properties of Kakdugi Fermentation (깍두기의 절임방법이 발효숙성 중 이화학적 특성에 미치는 영향)

  • 장명숙;김나영
    • Korean journal of food and cookery science
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    • v.15 no.1
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    • pp.61-67
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    • 1999
  • The effects of salting methods on Kakdugi (cubed radish kimchi) fermentation were evaluated. Kakdugi was prepared with various salting methods, salt concentrations, and settling times, and fermented at 10$^{\circ}C$ for up to 52 days. Radish (Raphanus sativus L.) cubes (2 cm size) were salted by using the following methods salt concentration of about 1.5% which was known appropriate for the organoleptic quality of Kakdugi: 1) Treatment S-1: applying dry salt uniformly onto the radish cubes, with a salt concentration of about 1.5% (w/w) and cured for 1 hr, 2) Treatment S-5: applying dry salt uniformly onto the radish cubes, with a salt concentration of about 1.2% (w/w) and cured for 5 hr, 3) Treatment B-1: brining radish cubes in a 8.5% (w/v) salt solution for 1 hr, 4) Treatment B-5: brining radish cubes in a 4.0% (w/v) salt solution for 5 hr. As the fermentation continued, the initial high decrease in pH has been retarded in all the treatments, of which the delaying extent was more significantly noted from B-1 and B-5 than S-1 and S-5. The pH of the Kakdugi which showed a good eating quality dropped to 4.3∼4.8 with the accumulation of total acids. Total vitamin C increased sharply at the palatable period of Kakdugi during the initial fermentation and then decreased gradually following a sigmoidal changing pattern. The reducing sugar levels were also influenced by salting methods and fermentation as sugars are converted into acids. High initial contents of reducing sugars and their subsequent rapid decrease were observed in “S” group than “B” group during fermentation. For nonvolatile organic acids, lactic acid increased consistently throughout the fermentation while malic acid, which was high at the initiation of fermentation, decreased rapidly afterwards at the palatable period of Kakdugi.

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Physiological Responses of Tomato Plants and Soil Microbial Activity in Salt Affected Greenhouse Soil

  • Sung, Jwakyung;Lee, Suyeon;Nam, Hyunjung;Lee, Yejin;Lee, Jongsik;Almaroai, Yaser A.;Ok, Yongsik
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.1065-1072
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    • 2012
  • Crop productivity decreases globally as a result of salinization. However, salinity impact on greenhouse-grown crops is much higher than on field-grown crops due to the overall concentrations of nutrients in greenhouse soils. Therefore, this study was performed to determine the short-term changes in growth, photosynthesis, and metabolites of tomato plants grown in greenhouse under heavily input of fertilizers evaluated by microbial activity and chemical properties of soils. The soils (< 3, 3.01~6, 6.01~10 and > 10.01 dS $m^{-1}$) from farmer's greenhouse fields having different fertilization practices were used. Results showed that the salt-accumulated soil affected adversely the growth of tomato plants. Tomato plants were seldom to complete their growth against > 10.0 dS $m^{-1}$ level of EC. The assimilation rate of $CO_2$ from the upper fully expanded leaves of tomato plants is reduced under increasing soil EC levels at 14 days, however; it was the highest in moderate or high EC-subjected (3.0 ~ 10.0 dS $m^{-1}$) at 28 days. In our experiment, soluble sugars and starch were sensitive markers for salt stress and thus might assume the status of crops against various salt conditions. Taken together, tomato plants found to have tolerance against moderate soil EC stress. Various EC levels (< 3.0 ~ 10.0 dS $m^{-1}$) led to a slight decrease in organic matter (OM) contents in soils at 28 days. Salinity stress led to higher microbial activity in soils, followed by a decomposition of OM in soils as indicated by the changes in soil chemical properties.

A Study on the Current Status and Needs of Nutrition Education on Children's Sugar Intake Reduction among the Center for Children's Foodservice Management and Child Care Facilities (어린이급식관리지원센터와 보육시설의 유아 당류 섭취 줄이기 영양교육 실태 및 요구도)

  • Kim, Mi-Hyun;Kim, Nam-Hee;Yeon, Jee-Young
    • The Korean Journal of Food And Nutrition
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    • v.30 no.3
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    • pp.539-551
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    • 2017
  • This study investigated the current status and needs for nutrition education to help reduce children's sugars intake at the Center for Children's Foodservice Management (CCFM, n=115), and Child Care Facilities (CCF, n=646) through an online survey conducted from October $5^{th}$ to $30^{th}$ 2015. A total of 14.8% of CCFM respondents and 31.9% of CCF respondents provided nutrition education on sugars intake to young children as a main topic (p<0.001). A higher percentage (CCFM 47.8%: CCF 42.4%) delivered nutrition education on sugars intake to young children as a sub-component (p<0.001). Over 90% of the CCFM and CCF participants agreed on the necessity of providing nutrition education on sugars intake to children. The most common reasons given for delivering nutrition education on children's sugar intake were "there are many more urgent nutrition education topics" for CCFM, and "insufficient nutrition education information and materials" for CCF. The percentage of nutrition education on children's sugar intake provided to the children's parents was low showing about 20% in the both groups. The percentage of CCFM participants providing nutrition, education on children's sugar intake to the teachers in CCF was also low, showing about 14.8%; however, 68.0% of the CCF participants wanted to received teacher's education on guiding children's sugar intake. Regarding ideas about a nutrition education program on children's sugar intake for young children, most respondents in both groups answered "sugar intake and dental cavities or obesity" for appropriate education contents, "story telling or puppet show" for appropriate education methods, and "dietitian from CCFM and class teacher together" for appropriate educator. For appropriate education time, there was a significantl difference between the CCFM responses (average 2.7 times) and the CCF responses (average 4 times). Based on the above results, we found that implementing nutrition education on children's sugar intake at the CCFM and CCF, was low; however, awareness of the need for nutrition education on children's sugar intake and the program development and supply was very high. Also, the opinions of CCFM and CCF participants about a nutrition education program on children's sugar intake for young children can provide foundation data to develop and implement the CCFM-based nutrition education program.

Development of Nutrition Education Materials for Prevention and Management of Diabetes Mellitus for Older Adults

  • Kim, Kyungwon;Hyunjoo Kang;Yun Ahn;Kim, Se-Hwa;Kim, Hee-Seon
    • Journal of Community Nutrition
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    • v.4 no.2
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    • pp.118-129
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    • 2002
  • Nutrition is important in the management of diabetes mellitus, however, there are few little education materials specifically designed for older adults. The objective of this study was to develop nutrition education materials for prevention and management of diabetes moll for older adults. Materials developed were a booklet and four leaflets. The contents of materials were based on lesson plans. After several revisions of the draft of materials, illustrations and icons appropriate to the contents were designed using illustrator 9.0 and Photoshop 6.0. The booklet was composed of five chapters and 40 pages. The first chapter began with an introduction about diabetes and diabetes management by diet, exercise and medication. The second chapter dealt with ideal body weight, calculation of adequate caloric intake and food exchange list. The third chapter provided information for meal planning and sample menus. The fourth chapter focused on practical tips on nutritional care of diabetes, by providing tips on reducing sugars, fat and salt, and suggestions on eating for special occasions. The fifth chapter dealt with information in case of low blood sugars, exercise and foot care. The topics of the four leaflets were “Diabetes, what is it and care”, “Food exchange list and meal planning”, “Healthy eating for diabetes”, “Special care for diabetes low blood sugars, exercise and foot care” Each leaflet was composed of six sections and was printed in large paper (B4 size) for older adults. The draft of educational materials were re-viewed by four nutrition professionals and finally pilot-tested with ten adults aged 50 and older. The characteristics of the developed materials are as follows, i) messages are delivered using simple, specific information, ⅱ) messages focused on practical applicable tips, ⅲ) various pictures, illustrations and artwork were created and inserted to enhance understanding and interest, ⅳ) sections including risk factor assessment, calculation of ideal body weight and meal planning were designed to induce the user's participation, ⅴ) sample menus and food pictures were inserted in the booklet, vi) characteristics of older adults and transformed characteristics are diversely used to help the user feel familiarity. These materials are self-explanatory and can be used by older adults. These materials also can be used widely in nutrition education at public health centers or senior centers.

Changes in Free Sugars and Organic Acids of Banana Fruit at Various Storage Temperatures (저장온도에 따른 바나나의 유리당과 유기산)

  • Lee, Kyoung-Ok;Choi, Jin-Young;Park, Sung-Oh;Lee, Taik-Soo
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.340-344
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    • 1995
  • Banana purchased from local market was stored at 5, 15 and $25^{\circ}C$ for 10 days after which free sugars and organic acids were determined. The amount of glucose, fructose and sucrose was $0.30{\sim}0.36%$ at the initial time of storage. The changes of free sugar was influenced by storage temperature. At $5^{\circ}C$, little change in the content of each free sugar was measured and sucrose had a slightly higher value. Glucose and fructose showed a predominant increase while sucrose content decreased at 15 and $25^{\circ}C$. The values of total sugar, reducing sugar, glucose, fructose and free sugar contents at $25^{\circ}C$ were higher than at any other storage temperature. Citrate, malate, lactate and acetate in Banana were identified as organic acids. The total amount of organic acid decreased from $305{\sim}373.3$ mg% at the initial time to $45.9{\sim}67.9$ mg% after 9 days of storage. The reduction of organic acids contents was relatively high at $25^{\circ}C$.

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