• Title/Summary/Keyword: S-Phase

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Termination Sites of fleplication Are Anchored to the Nuclear Matrix during S Phase in Mouse LPI-1 Cells (생쥐 LP1-1 세포에서 S phase 동안 nuclear matrix에 고정되어 있는 복제 끝점)

  • 이형호;이갑열
    • The Korean Journal of Zoology
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    • v.37 no.3
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    • pp.318-323
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    • 1994
  • The association of replication origins/termini with nuclear matrix during S phase was investigated by DNase digestion of halo structures in synchronized mouse LPI-1 cells. The binding of parental DNA to nuclear matrix was constant throughout S phase. When nuclear matrix was isolated from the cells pulse-labeled with 3H-thvmidine at various stases of S phase, total 3H-labels associated with nuclear matrix were specifically higher at So, Sa and Ss stages than other stases of S phase, suggesting that the newly synthesized DNAs at those stages are not excluded out of nuclear matrix. Similar patterns were obsenred from the pulse-chase experiments, in which cells were pulse-labeled at each stage of S phase and further incubated for 1 hr. These results suggest that the replication origins and termini are fixed at the nuclear matrix, and that the nuclear matrix binding fractions of DNA at 3C-pause may contain a large population of replication origins and termination sites.

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A Phase Detection Method For Line Lock (전원동기를 위한 위상검출방법)

  • Kim, Young-Choon;Lee, Sa-Young
    • Proceedings of the KIPE Conference
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    • 2007.07a
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    • pp.428-430
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    • 2007
  • Converter that is dc source equipment source's phase by reference control function that detect source's phase because should be done compulsorily use. Source's phase detect method there be method that use source's ac voltage directly by signal, and use methods that voltage detects status by PLL method and so on via point that '0' becomes usually. All above methods to detect phase are using, wrong action of phase detector converter's ailment or converter of burn can. Ths paper compares and examined usable phase detection method in source's frequency fluctuation presuming source's frequency using observer.

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Phase Separation and Precipitation Characteristics in ZnS doped Borosilicate Glasses (ZnS 미립자 분산 붕규산엽계 유리에서의 분상 및 미립자 석출 특성)

  • 이승환;류봉기
    • Journal of the Korean Ceramic Society
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    • v.35 no.12
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    • pp.1337-1342
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    • 1998
  • To investigate an effect of phase separation on precipitation characteristics of ZnS microcrystals in ma-trix glass ZnS doped borosilicate glasses for nonlinear optical applications were prepared by melting and pre-cipitation process. ZnS dopant contributed to phase separation promotion which increased the phase separa-tion of the matrix glass within immiscibility region. It was also found that ZnS as phase separation promoter showed a similar contribution for some selected glass compositions in miscibility region. The precipitation of ZnS microcrystals occurred in thephase separable glass compoitions. The radius of ZnS microcrystals in-creased with increasing the heat treatment temperature and Na2O contents of matrix glass composition. The ZnS particle sizes estimated by effective mass approximation ranged from about 30 to 80${\AA}$ It was suf-ficiently small to show quantum confinement effect.

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The Kinematic Analysis of Handspring Salto Forward Piked (핸드스프링 몸접어 앞공중돌기동작의 운동학적 분석)

  • Kwon, Oh-Seok
    • Korean Journal of Applied Biomechanics
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    • v.17 no.1
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    • pp.145-153
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    • 2007
  • The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

Nuclear Transplantation of Bovine IVF Embryos by Cell Cycle Control of Recipient Cytoplasm (수핵란 세포질의 세포주기 조절에 의한 소 체외수정란의 핵이식)

  • 정희태;임석기;박춘근;양부근;김정익
    • Korean Journal of Animal Reproduction
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    • v.20 no.3
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    • pp.307-313
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    • 1996
  • This study was conducted to investigate the effect of S-phase synthronized nuclear transfer on the development of nuclear transplant bovine embryos. A blastomere derived from the 16~32 cell stage bovine embryos was transferred into an enucleated metaphase II(MII) oocytes or activated S-phase eggs. From the MII-phase and S-phase nuclear transfer, 6.3%(4/63) and 13.8%(9/65) of nuclear transplant embryos developed to the blastocyst stage, respectively. In the S-phase nuclear transfer, maximal proportion of embryos developed to the blastocyst stage(16.6%) was obtained after the recipient cell was activated 8 h prior to receving a donor nucleus. MII-phase nuclear transplant embryos showed the PCC state of their nuclear at 1.5~2 h after fusion, whereas, S-phase nuclear transplant embryos did not undergo PCC. The result of this study suggests that if blastomeres of unknown cell-cycle-stage are used, S-phase nuclear transplantation through the activation of enucleated oocytes prior to fusion enhances development of nuclear transplant embryos. This result also suggests that the interval time from oocyte activation to cell fusion may affect the development of nuclear transplant embryos.

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An Efficient 3D Measurement Method that Improves the Fringe Projection Profilometry (Fringe Projection Profilometry를 개선한 효율적인 3D 측정 기법)

  • Kim, Ho-Joong;Cho, Tai-Hoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.10
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    • pp.1973-1979
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    • 2016
  • As technologies evolve, diverse 3D measurement techniques using cameras and pattern projectors have been developed continuously. In 3D measurement, high accuracy, fast speed, and easy implementation are very important factors. Recently, 3D measurement using multi-frequency fringe patterns for absolute phase computation has been widely used in the fringe projection profilometry. This paper proposes an improved method to compute the object's absolute phase using the reference plane's absolute phase and phase difference between the object and the reference plane. This method finds the object's absolute phase by adding the difference between the reference plane's wrapped phase and the object's wrapped phase to the reference plane's absolute phase already obtained in the calibration stage. Through this method, there is no need to obtain multi-frequency fringe patterns about new object for the absolute phase computation. Instead, we only need the object's phase difference relative to the reference planes's phase in the measurement stage.

Interaction Experiment on Chloride Ion Adsorption Behavior of C-S-H Phases (C-S-H 상의 염소이온 흡착 메커니즘 규명을 위한 반응 작용 실험)

  • Yoon, In-Seok
    • Journal of the Korea Concrete Institute
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    • v.29 no.1
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    • pp.65-75
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    • 2017
  • C-S-H phase is the most abundant reaction product, occupying about 50~60% of cement paste volume. The phase is also responsible for most of engineering properties of cement paste. This is not because it is intrinsically strong or stable, but because it forms a continuous layer that binds together the original cement particles into a cohesive whole. The binding ability of C-S-H phase arises from its nanometer-level structure. In terms of chloride penetration in concrete, C-S-H phase is known to adsorb chloride ions, however, its mechanism is very complicated and still not clear. The purpose of this study is to examine the interaction between chloride ions and C-S-H phase with various Ca/Si ratios and identify the adsorption mechanism. C-S-H phase can absorb chloride ions with 3 steps. In the C-S-H phase with low Ca/Si ratios, momentary physical adsorption could not be expected. Physical adsorption is strongly dependent on electro-kinetic interaction between surface area of C-S-H phase and chloride ions. For C-S-H phase with high Ca/Si ratio, electrical kinetic interaction was strongly activated and the amount of surface complexation increased. However, chemical adsorption could not be activated for C-S-H phase with high Ca/Si ratio. The reason can be explained in such a speculation that chloride ions cannot be penetrated and adsorbed chemically. Thus, the maximum chloride adsorption capacity was obtained from the C-S-H phase with a 1.50 Ca/Si ratio.

Strategies to Induce Metastable T' Phase of Monolayer MoS2

  • Gang, Seong-U
    • Proceeding of EDISON Challenge
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    • 2017.03a
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    • pp.417-423
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    • 2017
  • Monolayer $MoS_2$의 metastable phase인 T' phase가 stable한 H phase보다 안정해지는 조건을 모색하기 위해 substitutional doping을 했을 때와 strain을 걸어주었을 때의 에너지 차이를 DFT 방법으로 계산하였다. Doping을 했을 때와 strain이 있을 때 T' phase와 H phase의 에너지 차이가 감소함을 확인하였으나 H phase보다 T' phase가 안정해지는 조건을 찾지는 못하였다. 하지만 이 방법을 기존의 alkali adsorption 방법과 병행하여 기존 방법의 단점을 보완할 수 있을 것을 기대해 볼 수 있다. 또한 전자구조 분석 중 얻은 dopant의 주기와 족에 따른 경향성은 다른 TMD 물질의 phase engineering을 design할 때 universal한 design rule로서 응용할 수 있음을 기대해 볼 수 있다.

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Study on the Luminescence Properties according to ZnS multi-phase (ZnS multi-phase에 따른 발광특성 연구)

  • 김광복;김용일;천희곤;조동율;구경완
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.14 no.1
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    • pp.48-53
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    • 2001
  • The crystal structure of ZnS fabricated by gas-liquid phase reaction was refined by the Rietveld program using X-ray diffraction data. The R-weighted pattern (R$\sub$wp/) of ZnS powder was 10.85%. The fraction of HCP phase was closely related with extra amount of H$_2$S gas. The lattice parameters and crystalline size were changed by the relative ratio of multi-phase. The luminescence property of ZnS:Cu, Al green phosphors prepared by conventional methods was good in the range of 91∼94% and 150∼190${\AA}$, respectively. According to the maximum entropy electron density(MEED) methods, any defects in (001) plane of cubic phase were not found. We suggest that both the Rietveld and maximum entropy density methods may be useful tools for studying luminescence mechanism of other phosphors materials.

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A Study on Client's Decision-Making in Construction Phase of Building Projects in Japan

  • Tamura, Atsushi;Kaneta, Takashi;Furusaka, Shuzo
    • International conference on construction engineering and project management
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    • 2017.10a
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    • pp.183-191
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    • 2017
  • In building project, client needs to decide many things, and clients cooperate with architects, supervisors, general contractors and other consultants. Especially in construction phase, clients often make a decision. However, client's decision-making in construction phase often makes some kinds of risk. This paper aims to clarify the reality of client's decision-making in construction phase. First, client's decision-making is defined by laws and standards. Second, the questionnaire revealed the current status of client's decision-making in 4 works. Third, Fishbone diagram shows the factors of client's decision-making in four works. Finally, it was modeled how client's decision-making in construction phase affects the post-process of 4 works.

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