• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• Structural Engineering and Mechanics
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• 제85권2호
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• pp.179-195
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• 2023

The performance of reinforced concrete (RC) beam specimens strengthened using a newly proposed Side Near Surface Mounted (S-NSM) technology was investigated experimentally in this work. In addition, analytical and nonlinear finite element (FE) modeling was exploited to forecast the performance of RC members reinforced with S-NSM utilizing steel bars. Five (one control and four strengthened) RC beams were evaluated for flexural performance under static loading conditions employing four-point bending loads. Experimental variables comprise different S-NSM reinforcement ratios. The constitutive models were applied for simulating the non-linear material characteristics of used concrete, major, and strengthening reinforcements. The failure load and mode, yield and ultimate strengths, deflection, strain, cracking behavior as well as ductility of the beams were evaluated and discussed. To cope with the flexural behavior of the tested beams, a 3D non-linear FE model was simulated. In parametric investigations, the influence of S-NSM reinforcement, the efficacy of the S-NSM procedure, and the structural response ductility are examined. The experimental, numerical, and analytical outcomes show good agreement. The results revealed a significant increase in yield and ultimate strengths as well as improved failure modes.

• 한국자원식물학회지
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• 제14권3호
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• pp.196-205
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• 2001

NSM으로 무살균 재배를 여러 가지 배지를 혼합하여 균사생장과 밀도, 오염율을 실험한 결과는 다음과 같다. 첫째, 방울솜을 주재료로 하여 옥수수강과 비트, 미강을 각각 10, 20, 30, 40%를 혼합하였을 때 옥수수강, 미강, 비트 순으로 균사생장과 밀도가 양호한 결과를 보였으며 방울솜:옥수수강을 80 : 20(V/V)으로 혼합한 경우가 가장 양호하다. 특히 방울솜에 비트를 10% 이상 혼합하면 균사생장과 밀도가 현저히 불량한 특징이 있다. 둘째, 면자각을 주재료로 하여 옥수수강과 비트, 미강을 각각 10, 20, 30,40%를 혼합하였을 때 비트, 미강, 옥수수강 순으로 양호한 것으로 나타남에 따라 방울솜과 반대의 경향을 나타내었다. 특히 면자각에 미강을 20%이상 혼합하면 잡균오염도 높아질뿐만 아니라 균사생장과 밀도도 현저히 낮아지는 특징이 있다. 셋째, 방울솜을 주재료로 하여 옥수수강과 비트를 각각 혼합한 경 우 방울솜:옥수수강:비트를 70:20:10(V/V)로 혼합한 배지가 가장 양호 하였다. 넷째, 면자각을 주재료로 하여 옥수수강과 비트를 각각 혼합한 경우 면자각:옥수수강:비트를 70:20: 10(V/V)로 혼합한 배지가 가장 양호하였다. NSM의 적정 약제 농도별로는 500배가 가장 좋았으나 2,000배액에서도 균사생장은 비교적 좋은 결과를 나타내었으나 잡균오염율은 희석을 많이 하면 오염율이 높았다. NSM 배지의 적정수분함량은 77%가 적당하였으며, $25^{\circ}C$에서 배양하였을 때 균사밀도와균사속도가 양호하였다. NSM에 배지를 침지한 시간별로 조사한 결과 침지한 즉시 꺼내어 접종한 것이나 32시간 동안 침지한 것이나 동일한 효과가 있었으며 48시간 이상 침지하면 균사속도와 균사밀도가 약간 나빠지는 경향이 있었다. 따라서 NSM에 침지한 즉시 꺼내어 접종하면 균사생장과 균사밀도가 양호하였다. 즉 N.S.M을 이용하여 느타리버섯 폐면재배에 있어서 방울솜을 주재료로 하여 옥수수강, 비트, 미강을 혼합하여 균사생장, 균사밀도, 잡균오염도를 조사한 결과, 방울솜에 옥수수강을 20~30% 혼합하였을 때 균사생장이 76mm/10일 정도로 가장 양호하였고 균사밀도도 양호하였다.

• International Journal of Concrete Structures and Materials
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• 제10권2호
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• pp.149-161
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• 2016

This paper presents an experimental work to study the flexural strength of reinforced concrete (RC) beams strengthened by partially de-bonded near surface-mounted (NSM) fiber reinforced polymer (FRP) strip with various de-bonded length. Especially, considering high anchorage capacity at end of a FRP strip, the effect of de-bonded region at a central part was investigated. In order to check the improvement of strength or deformation capacity when the bonded surface area only increased without changing the FRP area, single and triple lines of FRP were planned. In addition, the flexural strength of the RC member strengthened by a partially de-bonded NSM FRP strip was evaluated by using the existing researchers' strength equation to predict the flexural strength after retrofit. From the study, it was found that where de-bonded region exists in the central part of a flexural member, the deformation capacity of the member is expected to be improved, because FRP strain is not to be concentrated on the center but to be extended uniformly in the de-bonded region. Where NSM FRP strips are distributed in triple lines, a relatively high strength can be exerted due to the increase of bond strength in the anchorage.

• Archives of Plastic Surgery
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• 제49권5호
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• pp.580-586
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• 2022

Background Appropriate position of the nipple-areolar complex (NAC) is crucial following nipple-sparing mastectomy (NSM). The prevention of NAC malposition in two-stage implant-based breast reconstruction has not been well described, and the efficacy of the techniques has not been evaluated. This study aimed to evaluate the efficacy of our technique to prevent NAC malposition in patients who underwent implant-based breast reconstruction after NSM. Methods Patients who underwent two-stage implant-based breast reconstruction with NSM between January 2012 and December 2019 were included. We used a surgical technique to fix the NAC to the rigid base, assuming a pocket-like appearance, with pectoralis major muscle and lateral adipofascial flap at the time of tissue expander (TE) insertion. Patients were classified into two groups based on the performance of the technique for the prevention of NAC malposition. Results In 35 patients who underwent implant-based breast reconstruction after NSM, the clavicle-to-nipple distance ratio was 96.0±5.0% in those who underwent NAC fixation and 86.1±11.5% in those who did not undergo NAC fixation. Conclusions Using our technique, NAC malposition could be prevented in two-stage implant-based breast reconstruction. NAC fixation during TE insertion was found to be extremely effective. This procedure successfully prevented NAC malposition without the formation of extra scars.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 봄 학술발표회 논문집
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• pp.549-554
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• 2003

In order to overcome the brittle failure of strengthening with FRP-rebars inherent to their brittle properties, two approaches have been attempted. One is to improve the properties like ductile Hybrid FRP Rods, and the other is to develop a ductile strengthening with partially unbonded FRP rebars. Experiments on real size specimen were performed to evaluate the performance of NSM (Near Surface Mounted Strengthening) and SIM (Section Increment Methods) with FRP rebars. This paper discusses the results of the tests on 8 slab specimen in terms of flexural resistance, ductility, and fatigue. They show that NSM or S1M with FRP rebars are very effective measures to strengthen existing RC structures. Above all, strengthening with partially unbonded ductile Hybrid FRP Rods shows sufficient ductility similar to that of properly designed RC structures.

• 근관절건강학회지
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• 제20권3호
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• pp.189-196
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• 2013

Purpose: The purpose of this study was to investigate electromyographic (EMG) activity of deep and superficial trunk muscles during trunk stabilization exercises with and without stabilization maneuvers. Methods: The relative muscle activity ratios and local muscle activities of 25 healthy males were measured using the 8 channel surface EMG system (Myosystem 1400A, Noraxon Inc., U.S.A). The surface EMG activities were tested during performing abdominal hollowing maneuver (AHM), abdominal bracing maneuver (ABM) and no stabilization maneuver (NSM) in random order. Data were analyzed using $1{\times}3$ repeated measures ANOVA. Results: During bridging exercises, the EMG activity ratio of transverse abdominis/internal oblique abdominis relative to rectus abdominis was significantly lower in NSM than in AHM and ABM. During bridging and kneeling exercises, the EMG activity ratio of multifidus relative to erector spinae was significantly higher in AHM than in NSM. Conclusion: The AHM can be clinically used by the physical therapist to activate selectively the trunk muscles when designing selective training programs for patients.

• Nuclear Engineering and Technology
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• 제53권10호
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• pp.3421-3430
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• 2021

Spherical NaI(Tl) detectors are used in gamma-ray spectrometry, where the gamma emissions come from the nuclei with energies in the range from a few keV up to 10 MeV. A spherical detector is aimed to give a good response to photons, which depends on their direction of travel concerning the detector center. Some distortions in the response of a gamma-ray detector with a different geometry can occur because of the non-uniform position of the source from the detector surface. The present work describes the calibration of a NaI(Tl) spherical detector using both an experimental technique and a numerical simulation method (NSM). The NSM is based on an efficiency transfer method (ETM, calculating the effective solid angle, the total efficiency, and the full-energy peak efficiency). Besides, there is a high probability for a source-to-detector distance less than 15 cm to have pulse coincidence summing (CS), which may occur when two successive photons of different energies from the same source are detected within a very short response time. Therefore, γ-γ ray CS factors are calculated numerically for a ¹⁵²Eu radioactive cylindrical source. The CS factors obtained are applied to correct the measured efficiency values for the radioactive volumetric source at different energies. The results show a good agreement between the NSM and the experimental values (after correction with the CS factors).

• Nuclear Engineering and Technology
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• 제54권4호
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• pp.1421-1430
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• 2022

The computation of the solid angle and the detector efficiency is considering to be one of the most important factors during the measuring process for the radioactivity, especially the cylindrical γ-ray NaI(Tl) detectors nowadays have applications in several fields such as industry, hazardous for health, the gamma-ray radiation detectors grow to be the main essential instruments in radiation protection sector. In the present work, a generic numerical simulation method (NSM) for calculating the efficiency of the γ-ray spectrometry setup is established. The formulas are suitable for any type of source-to-detector shape and can be valuable to determine the full-energy peak and the total efficiencies and P/T ratio of cylindrical γ-ray NaI(Tl) detector setup concerning the truncated conical radioactive source. This methodology is based on estimate the path length of γ-ray radiation inside the detector active medium, inside the source itself, and the self-attenuation correction factors, which typically use to correct the sample attenuation of the original geometry source. The calculations can be completed in general by using extra reasonable and complicate analytical and numerical techniques than the standard models; especially the effective solid angle, and the detector efficiency have to be calculated in case of the truncated conical radioactive source studied condition. Moreover, the (NSM) can be used for the straight calculations of the γ-ray detector efficiency after the computation of improvement that need in the case of γ-γ coincidence summing (CS). The (NSM) confirmation of the development created by the efficiency transfer method has been achieved by comparing the results of the measuring truncated conical radioactive source with certified nuclide activities with the γ-ray NaI(Tl) detector, and a good agreement was obtained after corrections of (CS). The methodology can be unlimited to find the theoretical efficiencies and modifications equivalent to any geometry by essential sufficiently the physical selective considered situation.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2022년도 제66차 하계학술대회논문집 30권2호
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• pp.19-22
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• 2022

In this work, we studied consumers' attractiveness/usefulness perceptions (CAUP) of online commerce product photos when exposed to alternative dark/light user interface (UI) modes. We analyzed time-series EEG data from 31 individuals and performed neuroscience mining (NSM) to ascertain (a) how the CAUP of products differs among UI modes; and (b) which deep learning model provides the most accurate assessment of such neuroscience mining (NSM) business difficulties. The dark UI style increased the CAUP of the products displayed and was predicted with the greatest accuracy using a unique EEG power spectra separated wave brainwave 2D-ConvLSTM model. Then, using relative importance analysis, we used this model to determine the most relevant power spectra. Our findings are considered to contribute to the discovery of objective truths about online customers' reactions to various user interface modes used by various online marketplaces that cannot be uncovered through more traditional research approaches like as surveys.