• Journal of the Korean Geotechnical Society
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• v.20 no.9
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• pp.177-189
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• 2004

The backfilled space carl have various shapes such as vertical or lateral symmetric, unsymmetric slope depending on field conditions. Kellogg (1993) suggested the different equations for the backfill earth pressure and the lateral stress ratio considering that the stresses are different between the symmetrically sloped backfilled space and the vertical one. Kellogg (1993) assumed the stress generated on sloped wall surface as the simple internal friction angle of backfilled soil. However, Moon (1997) suggested modified Kellogg equation assuming that stress behavior in the sloped wall will be varied according to the rotation angle of principal stress and the friction of sloped wall surface. This study has compared and investigated the horizontal stresss of unsymmetrical backfilled space numerically and experimentally obtained when Kellogg lateral stress ratio is appled to and when average lateral stress ratio considering unsymmetric backfill slop of left and right are applied to the modified Kellogg equation. It is shown that the horizontal stress on the sloped wall has good match numerically and experimentally in the modified Kellogg equation when Kellogg's lateral stress ratio in symmetric condition is applied to the unsymmetric condition. But the horizontal stress on the vertical wall shows disagreement numerically and experimentally. The horizontal stress results in good agreement numerically and experimentally when the average lateral stress ratio of left and right at unsymmetric slop as applied to the modified Kellogg equation. Therefore, it is estimated that the application of the average lateral stress ratio to the left and right wall should be considered when backfilled space formed unsymmetric conditions.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.701-705
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• 2002

This paper describes implementation of IPv6-IPv4 transition toolbox named as 6TALK(IPv6 TrAansLator of Krv6) and some scenarios using 6TALK which enables IPv6 island to connect other IPv6 island or IPv4 island seamlessly. 6TALK implements some transition mechanisms suggested in NGTrans Working Group of IETF. Those mechanisms are composed of basic mechanism, tunneling, and applied mechanism such as DSTM. 6TALK provides functions which enable IPv6 network at the edge of existing network to communicate with IPv4 network by using these transition mechanisms. As major transition mechanisms in 6TALK we adopt NAT-PT/SIIT and DSTM/DSTM options and as implementation environment we use Linux Kernel 2.4.18 and Netfilter framework. Software modules implemented in Linux kernel was ported to hardware box using Motorola MPC 8260 processor. The transition mechanisms used in 6TALK are the ones predicted to be used in initial transition step to IPv6.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.8
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• pp.1123-1132
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• 2018

In this paper, we design and implement a public bicycle sharing system based on smart phone application capable of distributing access codes via internet connection. When smartphone user uses the application to request a bicycle unlock code, server receives the request and sends an encrypted code, which is used to unlock the bicycle at the station and the same code is used to return the bicycle. The station's hardware prototypes were built on top of Internet devices such as raspberry pi, arduino, keypad, and motor driver, and smartphone application basically includes shared bike rental and return functionality. It also includes an additional feature of reservation for a certain time period. We tested the implemented system, and found that it is efficient because it shows the average of 3-4 seconds delay. The system can be implemented to manage multiple bikes with a single control box, and as the user can use a smartphone application, this makes the system more cost effective.

• Journal of the Korea Society of Computer and Information
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• v.15 no.7
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• pp.57-66
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• 2010

Due to the advancement of IPTV technologies, open IPTV services are a step closer to becoming reality. In such service environment, users are able to enjoy IPTV services using a variety of devices available at their home domain. However, it is impossible to get such flexible services at their convenience unless each of devices is individually connected to Set-Top-Box (STB) because of Conditional Access System (CAS) or service providers otherwise allow STB to freely distribute decoded contents to every user devices attached to STB. In this paper, we propose a key management scheme for securely distributing contents from STB to multiple user devices at home domain. The proposed scheme also makes the service providers be able to control the access rights to each of user devices without installing individual STBs. It is achieved by computationally dividing a private key of RSA signature scheme into three parts and thus makes possible to distribute the contents scrambled through a underlying CAS mechanism without re-encrypting them that the existing scheme should employ. It improves significantly computation and communication complexities, maintaining it as secure as the existing schemes. Additionally, it prevents misbehaving users from illegally distributing the contents from STB to their devices available at home domain.

• Journal of Life Science
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• v.27 no.12
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• pp.1479-1485
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• 2017

Muscle atrophy due to aging, starvation, and various chronic diseases leads to a decrease in muscle fiber area and density due to reduced muscle protein synthesis and increased protein breakdown. This study investigated the effect of dexamethasone and hydrogen peroxide on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes. C2C12 myoblasts were differentiated into myotubes in differentiation medium. During myoblast differentiation, muscle-specific transcription factors, such as myogenin, and MyoD expression increased. Differentiated C2C12 myotubes exposed to noncytotoxic levels of dexamethasone and hydrogen peroxide showed a decrease in myotube diameter, which was associated with up-regulation of muscle-specific ubiquitin ligases, such as muscle atrophy F-box (MAFbx)/atrogin-1 and muscle RING finger-1 (MuRF1), and down-regulation of myogenin and MyoD. These results demonstrated that dexamethasone and hydrogen peroxide induced atrophy through regulation of muscle-specific ubiquitin ligases and muscle-specific transcription factors in C2C12 myotubes. In this study, we confirmed the process of differentiation of C2C12 myoblasts into myotubes in in vitro experiments in the presence of atrophy. This muscle atrophy model of C2C12 cells induced by dexamethasone or hydrogen peroxide seems suited to studies of the mechanism of muscle atrophy suppression and to exploit the experiment for excavating new muscle atrophy.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.404-410
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• 2012

This study was conducted to examine effects of harvest time (09:00 vs. 14:00), precooling at $4^{\circ}C$ vs. no precooling, and storage temperature (4 vs. $8^{\circ}C$) on the storage life of 'Maehyang' strawberry fruits for export. Fruits at a 60% ripe stage were harvested from a commercial greenhouse in Gyeongsangnamdo, Jinju on May 4, 2010. Fruits were precooled by a forced draft cooling for three hours, transported for about 30 minutes and then stored, immediately. Small precoolers set in the farm were used for precooling. Fruits were placed in constant temperature chamber (4 or $8^{\circ}C$) after packaging using PVC wrap and a cardboard box. Fruits were examined for their changes in weight, hardness, Hunter color values, soluble solids content (SSC), and incidence of gray mold (Botrytis cinerea) during storage at a two days interval from May 6 to May 14, 2010. Hardness and SSC decreased as the ripening stage progressed. The Hunter's 'L' and 'a' value of fruit color decreased as time passed. Also, fresh weight decreased during storage at all temperatures. Soft rot appeared on epidermal tissues and followed by gray mold. Incidence of gray mold was greater at $8^{\circ}C$ storage temperature than in $4^{\circ}C$ storage temperature. However, no difference by the harvested time and precooling. The results indicate that effectiveness for keeping the freshness was best achieved by precooling at $4^{\circ}C$ and storage at $4^{\circ}C$, respectively.

• Journal of Embryo Transfer
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• v.32 no.1
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• pp.25-31
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• 2017

This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

• Restorative Dentistry and Endodontics
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• v.33 no.5
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• pp.428-434
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• 2008

The purpose of this study was to compare the microtensile bond strength in Class I cavities associated with different light curing modes of same light energy density. Occlusal enamel was removed to expose a flat dentin surface and twenty box-shaped Class I cavities were prepared in dentin. Single Bond (3M Dental product) was applied and Z 250 was inserted using bulk technique. The composite was light-cured using one of four techniques, pulse delay (PD group), soft-start (SS group), pulse cure (PC group) and standard continuous cure (CC group). The light-curing unit capable of adjusting time and intensity (VIP, Bisco Dental product) was selected and the light energy density for all curing modes was fixed at $16J/cm^2$. After storage for 24 hours, specimens were sectioned into beams with a rectangular cross-sectional area of approximately $1mm^2$ Microtensile bond strength $({\mu}TBS)$ test was per- formed using a univel·sal testing machine (EZ Test, Shimadzu Co.). The results were analyzed using oneway ANOVA and Tukey's test at significance level 0.05. The ${\mu}TBS$ of PD group and SS group was higher than that of PC group and CC group. Within the limitations of this in vitro study, modification of curing modes such as pulse delay and soft start polymerization can improve resin/dentin bond strength in Class I cavities by controlling polymerization velocity of composite resin.

• Journal of Korean Society of Steel Construction
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• v.17 no.4 s.77
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• pp.395-405
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• 2005

This paper summarizes the test results of the flexible-stiff mixed system for the effective use of high-strength steel. Steel with a high degree of strength and performance is being increasingly required as buildings get larger and taller. High-strength steels cannot be used for many applications, though, because they have a number of defects. For instance, they have a high yield ratio, a small strain in maximum stress, and equal Young's modulus compared to mild steels. A new structural system is needed to effectively use high-strength steels with some defects. This paper proposes the flexible-stiff mixed system for the effective use of high-strength steels with high yield ratios. The possibility of using the system is discussed through the test of flexible-stiff mixed columns with high-strength steels. The main variable of the specimens is the yield displacement ratio, including both the force ratio and the stiffness ratio. The proper yield displacement ratio is proposed by adopting the flexible-stiff mixed system. The test results showed that the proposed flexible-stiff mixed system has a high capacity for energy absorption and the highest capacity for energy absorption when the yield displacement ratio of the flexible element to the stiff element ranges from 2.7 to 3.3.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.06a
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• pp.67-86
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• 2001

A strain producing strongly fibrinolytic enzyme was isolated from soil and was identified to be Bacillus subtilis by biochemical and physiological characterization. The optimal culture conditions for the production of fibrinolytic enzyme was determined to be 1.0% tryptone, 1.5% soluble starch, 0.5% Peptone, 0.5% NaCl, $(NH_{4})_{3}PO_4.3H_{2}O, and MgSO_{4}.7H_{2}O.$ Initial pH and temperature were pH 8.0 and $30^{\circ}C$ , respectively, The highest enzyme production was observed at 30 hours of cultivation at $30^{\circ}C$ The fibrinolytic enzyme was purified to homogeneity by DEAE Sephadex A-50 ion exchange column chromatography, 70% ammonium sulfate precipitation, Sephadex G-200 and G-75 gel filtration column chromatography. The molecular weight of the purified enzyme was 28,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A gene encoding the fibrinolytic enzyme was cloned into a plasmid vector pBluescript, transforming E.coli XL-1 Blue. The clone was able to degrade fibrin, This indicated that the gene could encode a fibrinolytic enzyme. The nucleotide sequence of the 2.7 kb insert was determined in both direction. One open reading frame composed of 1023 nucleotides was found to be a potential protein coding region. There was the putative Shine-Dalgano sequence and TATA box upstream of the open reading frame. The homology search data in the genome database showed that both the 2.7 kb insert and 1 kb open reading frame carried no significance in the nucleotide sequence of known fibrinolytic enzyme from Bacillus serovars. The recombinant cell harboring the novel gene involved in fibrinolysis was subjected to protein purification. The molecular mass of the purified fibrinolytic enzyme was determined to be 31864 Dalton, which was highly in accordance with the molecular mass(33 kDa) of the fibrinolytic gene deduced from the insert. The fibrinolytic enzyme was Purified 50.5 folds to homogeneity in overall yield of 10.7% by DEAE Sephadex A-50 ion exchange, 85% ammonium sulfate precipitation, Sephadex G-50, Superdex 75 HR FPLC gel filtration. In conclusion, a novel fibrinolytic gene from Bacillus subtilis was identified and characterized by cloning a genomic library of Bacillus subtilis into pBleuscript. For the soybean fermented by this strain, it is found that there increased assistant protein about 20% compared to the soybean not fermented and increased about 30% according to amino acid analysis and, in particular, essential amino acid increased about 40%. When keeping this fermented soybean powder at room temperature for about 70days, it showed very high stability maintaining almost perfect activity and, therefore, it gave us great suggestion its possibility of development as a new functional food.