• Title/Summary/Keyword: S-247

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Identification of Salted Opossum Shrimp Using COI-based Restriction Fragment Length Polymorphism (COI 기반 제한효소 절편 길이 다형성(RFLP)을 이용한 새우젓 분석)

  • Park, Ju Hyeon;Moon, Soo Young;Kang, Ji Hye;Jung, Myoung Hwa;Kim, Sang Jo;Choi, Hee Jung
    • Journal of Life Science
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    • v.31 no.1
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    • pp.66-72
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    • 2021
  • This study developed a species identification method for the salted opossum shrimp of Acetes japonicus, A. chinensis (Korea, China), A. indicus (I, II), and Palaemon gravieri based on PCR-RFLP markers. Genomic DNA was extracted from the salted opossum shrimp. The COI gene was used to amplify 519 base pairs (bp) using specific primers. The amplified products were digested by Acc I and Hinf I, and the DNA fragments were separated by automated electrophoresis for RFLP analysis. When the amplified DNA product (519 bp) was digested with Acc I, A. japonicus, A. chinensis (Korea), and A. indius (II) showed two fragments, whereas a single band of 519 bp was detected in A. chinensis (China) and A. indius (I). Also, in the RFLP patterns digested by Hinf I, A. chinensis (Korea) and A. chinensis (China) showed a single band of 519 bp, while two fragments were observed in A. japonicus and A. indius (I) and four fragments in A. indius (II). The PCR amplicon of P. gravieri was digested by Acc I into 3 bands of 271, 202, and 46 bp and by Hinf I into a single band of 519 bp. Therefore, salted opossum shrimp-specific RFLP markers showing distinct differences between four species and two sub-species by PCR-RFLP analysis. Thus, the PCR-RFLP markers developed in this study are a good method for identifying the six types of salted opossum shrimp.

Reduction of headache intensity and frequency with maxillary stabilization splint therapy in patients with temporomandibular disorders-headache comorbidity: a systematic review and meta-analysis

  • Manrriquez, Salvador L.;Robles, Kenny;Pareek, Kam;Besharati, Alireza;Enciso, Reyes
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.21 no.3
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    • pp.183-205
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    • 2021
  • This systematic review and meta-analysis aimed to analyze the effectiveness of maxillary stabilization splint (SS) therapy to reduce headache (HA) intensity and HA frequency in patients with temporomandibular disorders (TMD)-HA comorbidity. Randomized controlled trials (RCTs) using full-arch coverage, hard resin, and maxillary SS therapy were included. Electronic databases, including Cochrane Library, MEDLINE through PubMed, Web of Science, and EMBASE, were searched. The risk of bias was analyzed based on Cochrane's handbook. The search yielded 247 references up to January 28, 2020. Nine RCTs were included at a high risk of bias. The comparison groups included other splints, counseling, jaw exercises, medications, neurologic treatment, and occlusal equilibration. Four studies reported a statistically significant reduction in HA intensity, and five studies reported significant improvement in HA frequency from baseline at 2-12 months in patients with TMD-HA comorbidity treated with a full-arch hard maxillary SS. HA frequency in tension-type HA (TTH) comorbid with TMD diagnoses of myofascial pain (MFP) or capsulitis/synovitis improved significantly with SS than that with full-arch maxillary non-occluding splint (NOS) in two studies. Comparison groups receiving hard partial-arch maxillary splint nociceptive trigeminal inhibition (NTI) showed statistically significant improvements in HA intensity in patients with mixed TMD phenotypes of MFP and disc displacement comorbid with "general HA." Comparison groups receiving partial-arch maxillary resilient/soft splint (Relax) showed significant improvements in both HA intensity and frequency in patients with HA concomitant with MFP. The meta-analysis showed no statistically significant difference in the improvement of pain intensity at 2-3 months with comparison of the splints (partial-arch soft [Relax], hard [NTI], and full-arch NOS) or splint use compliance at 6-12 months with comparison of the splints (partial-arch Relax and full-arch NOS) versus the SS groups in patients with various TMD-HA comorbidities. In conclusion, although SS therapy showed a statistically significant decrease in HA intensity and HA frequency when reported, the evidence quality was low due to the high bias risk and small sample size. Therefore, further studies are required.

An In-depth Analysis and Future Prospect of Korean Educational Administration Research Trends : Focused on the Study of Educational Administration from 2009 to 2018 (한국 교육행정학 연구동향의 심층분석 및 미래 전망 : 2009년~2018년까지의 교육행정학연구를 중심으로)

  • Shin, Hyun-Seok;Park, Gyun-Yeol;LEE, YE SEUL;Yun, Ji-Hui;Sin, Beom-Cheol
    • (The)Korea Educational Review
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    • v.24 no.4
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    • pp.247-286
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    • 2018
  • The study aims to analyze the research trends according to 'characteristics of researchers' in the research papers published in "The Journal of Educational Administration" between last 10 years(2009 to 2018 first half). The study results are as follow. First, in terms of the researcher, more research was done by 'joint' researchers than a 'single' researcher. In addition, the number of studies in which the first author was male was higher than in women. Also, collaborative research between 'professors' and 'graduate students' was actively done. Second, the number of studies to develop and verify theories(or models) has increased. Third, in terms of research methods, more studies tend to utilize 'quantitative research' using advanced statistics and 'qualitative research' through interviews and observations to describe and explain Korea's educational administration phenomenon. Fourth, researchers showed high interest in 'School organization and management;', 'Higher education', 'Education policy and planning'. Fifth, according to each research area, there were some differences in the characteristics of the researchers who conducted the research, the nature of the research, and the research methods used mainly. Based on the results of this study, this study suggested the future trends of educational administration research in the era of the fourth industrial revolution.

Comparative study of antioxidant and anti-neuroinflammatory activity of leaf extracts of three different species of Bamboos in different extraction solvents containing caffeic acid, p-coumaric acid and tricin (왕대, 조릿대, 오죽의 추출 용매에 따른 항산화, 신경염증제어 활성 및 지표성분 caffeic acid, p-coumaric acid, tricin의 함량 비교)

  • Kim, Yon-Suk;Cho, Duk-Yeon;Kim, Mikyung;Choi, Dong-Kug
    • Korean Journal of Food Science and Technology
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    • v.53 no.3
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    • pp.296-303
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    • 2021
  • The antioxidant and anti-neuroinflammatory activities of water, 30, 70, and 100% ethanol extracts of leaves of three different species of bamboo (Phyllostachys nigra, P. bambusoides, and Sasa borealis) were investigated. The levels of total polyphenol and flavonoid were measured, and antioxidant activity was evaluated using various antioxidant assays (DPPH, ABTS, and FRAP). Lipopolysaccharide (LPS)-induced BV2 microglial cell activation was used to evaluate the anti-neuroinflammatory properties of the bamboo leaf extracts. Treatment with both aqueous and ethanolic extracts showed no cytotoxicity in BV-2 microglial cells. Pre-treatment of BV-2 cells with bamboo leaf extracts significantly inhibited LPS-induced excessive production of nitric oxide in a dose-dependent manner. Moreover, phytochemical analysis based on the extraction solvent showed that caffeic acid, p-coumaric acid, and tricin are the principal constituents of all three bamboo leaf extracts. Therefore, our findings suggest that bamboo leaf extract contains potent antioxidants and anti-neuroinflammatory compounds that can be used as potential therapeutic agents for the treat neuroinflammatory diseases.

Factors Affecting Nurses' Performance of Standard Precautions for Infection in Public Medical Institutions: Focusing on Knowledge and Safety Environment (공공의료기관 간호사의 감염 표준주의지침 수행도 영향요인 : 지식, 안전환경 중심으로)

  • Lee, Ji-in;Kim, Jong Kyung
    • The Journal of the Korea Contents Association
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    • v.21 no.7
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    • pp.238-247
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    • 2021
  • This study is a descriptive research to investigate the, safety environment, and performance of nurses working in public medical institutions on standard precautions for infection, and to identify factors affecting the performance of standard precautions for infection. As for the research method, data were collected through a questionnaire on 192 nurses from two public medical institutions in S city and N city, and the results were analyzed using the SPSS WIN 22.0 statistical program. From the research results, there was a significant difference in the knowledge of standard precautions according to work experience, and there was a significant difference in the safety environment of standard precautions according to the position. The average level of knowledge of standard precautions was 21.98/25 points, the average safety environment was 5.68/7 points, and the average performance was 40.28/60 points. There was a significant positive correlation between the safety environment of standard precautions and the performance of standard precautions (r=.21, p<0.01). The affecting factor on the performance of standard precautions was found to be the safety environment of standard precautions(𝛽=.22, p=.003). Therefore, to improve the performance of standard precautions for nurses' infection control in public medical institutions, Different and persistent infection management educational programs that consider the Historical situation and monitoring are required other than providing financial support and supportive safety environment.

Introduction, Development, and Characterization of Supernodulating Soybean Mutant. 1. Mutagenesis of Soybean and Selection of Supernodulating Soybean Mutant (다량 뿌리혹 형성 콩 계통의 도입 개발 및 생육특성구명 1. 돌연변이유기에 의한 콩 초다뿌리혹형성 계통선발)

  • Lee, Hong-Suk;Chae, Young-Am;Park, Eui-Ho;Kim, Yong-Wook;Yun, Kwang-Il;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.247-253
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    • 1997
  • Development of soybean cultivars with great nodulation and high nitrogen fixation activity, derived mostly from mutagenesis, may decrease inputs of chemical fertilizer nitrogen into the soil-plant system. Soybean seeds (cv. Jangyupkong, Hwanggeumkong, and Geomjungkong 1) were treated with three different levels of EMS (ethyl methanesulfonate) concentration(30, 50, and 70mM). Increasing the doses of EMS resulted in decreased field emergence rate of seeds, whereas it did not increase M$_2$ mutation frequencies. This indicated that the most efficient concentration of EMS was 30mM for generating mutants. Extensive mutagenesis of Sinpaldalkong 2 with 30mM EMS was undertaken to isolate soybean mutants with greater nodulation. Approximately 8, 200 M$_2$ families were screened for greater nodulation on 5 mM nitrate after inoculation with Bradyrhizobium japonicum strain YCK213-KFCC-10728. Mutant SS-2 nodulated more than the wild type. Comparison of supernodulation between SS-2 and two nts mutants(nts 1007 and nts 1116) revealed that SS-2 showed the supernodulation character at an earlier growth stage than the two nts mutants. Further studies should be needed to characterize the difference in timing of nodulation between SS-2 and nts mutants.

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Enhancement of durability of tall buildings by using deep-learning-based predictions of wind-induced pressure

  • K.R. Sri Preethaa;N. Yuvaraj;Gitanjali Wadhwa;Sujeen Song;Se-Woon Choi;Bubryur Kim
    • Wind and Structures
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    • v.36 no.4
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    • pp.237-247
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    • 2023
  • The emergence of high-rise buildings has necessitated frequent structural health monitoring and maintenance for safety reasons. Wind causes damage and structural changes on tall structures; thus, safe structures should be designed. The pressure developed on tall buildings has been utilized in previous research studies to assess the impacts of wind on structures. The wind tunnel test is a primary research method commonly used to quantify the aerodynamic characteristics of high-rise buildings. Wind pressure is measured by placing pressure sensor taps at different locations on tall buildings, and the collected data are used for analysis. However, sensors may malfunction and produce erroneous data; these data losses make it difficult to analyze aerodynamic properties. Therefore, it is essential to generate missing data relative to the original data obtained from neighboring pressure sensor taps at various intervals. This study proposes a deep learning-based, deep convolutional generative adversarial network (DCGAN) to restore missing data associated with faulty pressure sensors installed on high-rise buildings. The performance of the proposed DCGAN is validated by using a standard imputation model known as the generative adversarial imputation network (GAIN). The average mean-square error (AMSE) and average R-squared (ARSE) are used as performance metrics. The calculated ARSE values by DCGAN on the building model's front, backside, left, and right sides are 0.970, 0.972, 0.984 and 0.978, respectively. The AMSE produced by DCGAN on four sides of the building model is 0.008, 0.010, 0.015 and 0.014. The average standard deviation of the actual measures of the pressure sensors on four sides of the model were 0.1738, 0.1758, 0.2234 and 0.2278. The average standard deviation of the pressure values generated by the proposed DCGAN imputation model was closer to that of the measured actual with values of 0.1736,0.1746,0.2191, and 0.2239 on four sides, respectively. In comparison, the standard deviation of the values predicted by GAIN are 0.1726,0.1735,0.2161, and 0.2209, which is far from actual values. The results demonstrate that DCGAN model fits better for data imputation than the GAIN model with improved accuracy and fewer error rates. Additionally, the DCGAN is utilized to estimate the wind pressure in regions of buildings where no pressure sensor taps are available; the model yielded greater prediction accuracy than GAIN.

Essay Teaching Method of the Moral Education in the Elementary School Based on Philosophy and Discussion : focusing on the concept analysis method (철학과 토론에 기초한 초등학교 도덕교육에서의 논술수업방안 - 개념분석모형을 중심으로 -)

  • Jang, Seung-Hee
    • Journal of Ethics
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    • no.72
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    • pp.247-272
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    • 2009
  • The purpose of this study is to suggest a direction, contents, and method of essay education in the elementary school. The value of essay is getting weaker and weaker as a evaluation item of a university entrance examination. But there is a lot of educational value of essay itself yet. The essay goes beyond merely writing, as long as it displays one's sense of values and viewpoint of world. It is the highest intelligent ability that not is formated easily in short time. Starting a essay education at elementary school age is better than middle or high school age. when the students in a elementary school, they have a little burden of studies. The elementary students can be educated to develop the power of philosophical thought focusing on the reading and discussing. The moral subject have to assign the essay education, because thinking faculty is very important ability in essay. This study consisted of three directions ; firstly, what is direction of elementary moral education for essay education? secondly, what is contents and method as a basis of essay education in the moral subject? thirdly, how does teach the concept analysis as a concrete essay education method? I suggested answers in the three aspects of moral education in the elementary school. The essay education is important as the result of moral education, philosophy education as contents, and discuss training as a method. And I applicated concretely contents of this study as a concept analysis method in class.

Studies on the Derivation of the Instantaneous Unit Hydrograph for Small Watersheds of Main River Systems in Korea (한국주요빙계의 소유역에 대한 순간단위권 유도에 관한 연구 (I))

  • 이순혁
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.19 no.1
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    • pp.4296-4311
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    • 1977
  • This study was conducted to derive an Instantaneous Unit Hydrograph for the accurate and reliable unitgraph which can be used to the estimation and control of flood for the development of agricultural water resources and rational design of hydraulic structures. Eight small watersheds were selected as studying basins from Han, Geum, Nakdong, Yeongsan and Inchon River systems which may be considered as a main river systems in Korea. The area of small watersheds are within the range of 85 to 470$\textrm{km}^2$. It is to derive an accurate Instantaneous Unit Hydrograph under the condition of having a short duration of heavy rain and uniform rainfall intensity with the basic and reliable data of rainfall records, pluviographs, records of river stages and of the main river systems mentioned above. Investigation was carried out for the relations between measurable unitgraph and watershed characteristics such as watershed area, A, river length L, and centroid distance of the watershed area, Lca. Especially, this study laid emphasis on the derivation and application of Instantaneous Unit Hydrograph (IUH) by applying Nash's conceptual model and by using an electronic computer. I U H by Nash's conceptual model and I U H by flood routing which can be applied to the ungaged small watersheds were derived and compared with each other to the observed unitgraph. 1 U H for each small watersheds can be solved by using an electronic computer. The results summarized for these studies are as follows; 1. Distribution of uniform rainfall intensity appears in the analysis for the temporal rainfall pattern of selected heavy rainfall event. 2. Mean value of recession constants, Kl, is 0.931 in all watersheds observed. 3. Time to peak discharge, Tp, occurs at the position of 0.02 Tb, base length of hlrdrograph with an indication of lower value than that in larger watersheds. 4. Peak discharge, Qp, in relation to the watershed area, A, and effective rainfall, R, is found to be {{{{ { Q}_{ p} = { 0.895} over { { A}^{0.145 } } }}}} AR having high significance of correlation coefficient, 0.927, between peak discharge, Qp, and effective rainfall, R. Design chart for the peak discharge (refer to Fig. 15) with watershed area and effective rainfall was established by the author. 5. The mean slopes of main streams within the range of 1.46 meters per kilometer to 13.6 meter per kilometer. These indicate higher slopes in the small watersheds than those in larger watersheds. Lengths of main streams are within the range of 9.4 kilometer to 41.75 kilometer, which can be regarded as a short distance. It is remarkable thing that the time of flood concentration was more rapid in the small watersheds than that in the other larger watersheds. 6. Length of main stream, L, in relation to the watershed area, A, is found to be L=2.044A0.48 having a high significance of correlation coefficient, 0.968. 7. Watershed lag, Lg, in hrs in relation to the watershed area, A, and length of main stream, L, was derived as Lg=3.228 A0.904 L-1.293 with a high significance. On the other hand, It was found that watershed lag, Lg, could also be expressed as {{{{Lg=0.247 { ( { LLca} over { SQRT { S} } )}^{ 0.604} }}}} in connection with the product of main stream length and the centroid length of the basin of the watershed area, LLca which could be expressed as a measure of the shape and the size of the watershed with the slopes except watershed area, A. But the latter showed a lower correlation than that of the former in the significance test. Therefore, it can be concluded that watershed lag, Lg, is more closely related with the such watersheds characteristics as watershed area and length of main stream in the small watersheds. Empirical formula for the peak discharge per unit area, qp, ㎥/sec/$\textrm{km}^2$, was derived as qp=10-0.389-0.0424Lg with a high significance, r=0.91. This indicates that the peak discharge per unit area of the unitgraph is in inverse proportion to the watershed lag time. 8. The base length of the unitgraph, Tb, in connection with the watershed lag, Lg, was extra.essed as {{{{ { T}_{ b} =1.14+0.564( { Lg} over {24 } )}}}} which has defined with a high significance. 9. For the derivation of IUH by applying linear conceptual model, the storage constant, K, with the length of main stream, L, and slopes, S, was adopted as {{{{K=0.1197( {L } over { SQRT {S } } )}}}} with a highly significant correlation coefficient, 0.90. Gamma function argument, N, derived with such watershed characteristics as watershed area, A, river length, L, centroid distance of the basin of the watershed area, Lca, and slopes, S, was found to be N=49.2 A1.481L-2.202 Lca-1.297 S-0.112 with a high significance having the F value, 4.83, through analysis of variance. 10. According to the linear conceptual model, Formular established in relation to the time distribution, Peak discharge and time to peak discharge for instantaneous Unit Hydrograph when unit effective rainfall of unitgraph and dimension of watershed area are applied as 10mm, and $\textrm{km}^2$ respectively are as follows; Time distribution of IUH {{{{u(0, t)= { 2.78A} over {K GAMMA (N) } { e}^{-t/k } { (t.K)}^{N-1 } }}}} (㎥/sec) Peak discharge of IUH {{{{ {u(0, t) }_{max } = { 2.78A} over {K GAMMA (N) } { e}^{-(N-1) } { (N-1)}^{N-1 } }}}} (㎥/sec) Time to peak discharge of IUH tp=(N-1)K (hrs) 11. Through mathematical analysis in the recession curve of Hydrograph, It was confirmed that empirical formula of Gamma function argument, N, had connection with recession constant, Kl, peak discharge, QP, and time to peak discharge, tp, as {{{{{ K'} over { { t}_{ p} } = { 1} over {N-1 } - { ln { t} over { { t}_{p } } } over {ln { Q} over { { Q}_{p } } } }}}} where {{{{K'= { 1} over { { lnK}_{1 } } }}}} 12. Linking the two, empirical formulars for storage constant, K, and Gamma function argument, N, into closer relations with each other, derivation of unit hydrograph for the ungaged small watersheds can be established by having formulars for the time distribution and peak discharge of IUH as follows. Time distribution of IUH u(0, t)=23.2 A L-1S1/2 F(N, K, t) (㎥/sec) where {{{{F(N, K, t)= { { e}^{-t/k } { (t/K)}^{N-1 } } over { GAMMA (N) } }}}} Peak discharge of IUH) u(0, t)max=23.2 A L-1S1/2 F(N) (㎥/sec) where {{{{F(N)= { { e}^{-(N-1) } { (N-1)}^{N-1 } } over { GAMMA (N) } }}}} 13. The base length of the Time-Area Diagram for the IUH was given by {{{{C=0.778 { ( { LLca} over { SQRT { S} } )}^{0.423 } }}}} with correlation coefficient, 0.85, which has an indication of the relations to the length of main stream, L, centroid distance of the basin of the watershed area, Lca, and slopes, S. 14. Relative errors in the peak discharge of the IUH by using linear conceptual model and IUH by routing showed to be 2.5 and 16.9 percent respectively to the peak of observed unitgraph. Therefore, it confirmed that the accuracy of IUH using linear conceptual model was approaching more closely to the observed unitgraph than that of the flood routing in the small watersheds.

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Upper Boundary Line Analysis of Rice Yield Response to Meteorological Condition for Yield Prediction I. Boundary Line Analysis and Construction of Yield Prediction Model (최대경계선을 이용한 벼 수량의 기상반응분석과 수량 예측 I. 최대경계선 분석과 수량예측모형 구축)

  • 김창국;이변우;한원식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.3
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    • pp.241-247
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    • 2001
  • Boundary line method was adopted to analyze the relationships between rice yield and meteorological conditions during rice growing period. Boundary lines of yield responses to mean temperature($T_a$) and sunshine hour( $S_{h}$) and diurnal temperature range($T_r$) were well-fitted to hyperbolic functions of f($T_a$) =$$\beta$_{0t}$(1-EXP(-$$\beta$_{1t}$ $\times$ ($T_a$) ) and f( $S_{h}$)=$$\beta$_{0t}$((1-EXP($$\beta$_{1t}$$\times$ $S_{h}$)), to quadratic function of f($T_r$) =$\beta$$_{0r}$(1-($T_r$ 1r)$^2$), respectively. to take into account to, the sterility caused by low temperature during reproductive stage, cooling degree days [$T_c$ =$\Sigma$(20-$T_a$] for 30 days before heading were calculated. Boundary lines of yield responses to $T_c$ were fitted well to exponential function of f($T_c$) )=$\beta$$_{0c}$exp(-$$\beta$_{1c}$$\times$$T_c$ ). Excluding the constants of $\beta$$_{0s}$ from the boundary line functions, formed are the relative function values in the range of 0 to 1. And these were used as yield indices of the meteorological elements which indicate the degree of influence on rice yield. Assuming that the meteorological elements act multiplicatively and independently from each other, meteorological yield index (MIY) was calculated by the geometric mean of indices for each meteorological elements. MIY in each growth period showed good linear relationship with rice yield. The MIY's during 31 to 45 days after transplanting(DAT) in vegetative stage, during 30 to 16 days before heading (DBH) in reproductive stage and during 20 days after heading (DAH) in ripening stage showed greater explainablity for yield variation in each growth stage. MIY for the whole growth period was calculated by the following three methods of geometric mean of the indices for vegetative stage (MIVG), reproductive stage (HIRG) and ripening stage (HIRS). MI $Y_{I}$ was calculated by the geometric mean of meteorological indices showing the highest determination coefficient n each growth stage of rice. That is, (equation omitted) was calculated by the geometric mean of all the MIY's for all the growth periods devided into 15 to 20 days intervals from transplanting to 40 DAH. MI $Y_{III}$ was calculated by the geometric mean of MIY's for 45 days of vegetative stage (MIV $G_{0-45}$ ), 30 days of reproductive stage (MIR $G_{30-0}$) and 40 days of ripening stage (MIR $S_{0-40}$). MI $Y_{I}$, MI $Y_{II}$ and MI $Y_{III}$ showed good linear relationships with grain yield, the coefficients of determination being 0.651, 0.670 and 0.613, respectively.and 0.613, respectively.

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