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http://dx.doi.org/10.5352/JLS.2021.31.1.66

Identification of Salted Opossum Shrimp Using COI-based Restriction Fragment Length Polymorphism  

Park, Ju Hyeon (General Affairs Division, National Fishery Products Quality Management Service)
Moon, Soo Young (General Affairs Division, National Fishery Products Quality Management Service)
Kang, Ji Hye (General Affairs Division, National Fishery Products Quality Management Service)
Jung, Myoung Hwa (General Affairs Division, National Fishery Products Quality Management Service)
Kim, Sang Jo (General Affairs Division, National Fishery Products Quality Management Service)
Choi, Hee Jung (General Affairs Division, National Fishery Products Quality Management Service)
Publication Information
Journal of Life Science / v.31, no.1, 2021 , pp. 66-72 More about this Journal
Abstract
This study developed a species identification method for the salted opossum shrimp of Acetes japonicus, A. chinensis (Korea, China), A. indicus (I, II), and Palaemon gravieri based on PCR-RFLP markers. Genomic DNA was extracted from the salted opossum shrimp. The COI gene was used to amplify 519 base pairs (bp) using specific primers. The amplified products were digested by Acc I and Hinf I, and the DNA fragments were separated by automated electrophoresis for RFLP analysis. When the amplified DNA product (519 bp) was digested with Acc I, A. japonicus, A. chinensis (Korea), and A. indius (II) showed two fragments, whereas a single band of 519 bp was detected in A. chinensis (China) and A. indius (I). Also, in the RFLP patterns digested by Hinf I, A. chinensis (Korea) and A. chinensis (China) showed a single band of 519 bp, while two fragments were observed in A. japonicus and A. indius (I) and four fragments in A. indius (II). The PCR amplicon of P. gravieri was digested by Acc I into 3 bands of 271, 202, and 46 bp and by Hinf I into a single band of 519 bp. Therefore, salted opossum shrimp-specific RFLP markers showing distinct differences between four species and two sub-species by PCR-RFLP analysis. Thus, the PCR-RFLP markers developed in this study are a good method for identifying the six types of salted opossum shrimp.
Keywords
Mitochondrial DNA; PCR-RFLP; salted opossum shrimp; species identification;
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1 Akasaki, T., Yanagimoto, T., Yamakami, K., Tomonaga, H., and Sato, S. 2006. Species identification and PCR-RFLP analysis of cytochrome b gene in cod fish (order Gadiformes) products. J. Food Sci. 71, 190-195.
2 Alexander, L. V., Anastasia, A. L. and Jorgen, O. 2016. Phylogeny and classification of the shrimp genera Acetes, Peisos, and Sicyonella (Sergestidae: Crustacea: Decapoda). Zool. J. Linn. Soc. 177, 353-377.   DOI
3 Avise, J. C., Arnold, J., Ball, R. M., Bermingham, E., Lamb, T., Neigel, J. E., Reeb, C. A. and Sauders, C. V. 1987. Interspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Ann. Rev. Ecol. Syst. 18, 489-522.   DOI
4 Baldwin, J. D., Bass, A. L., Bowen, B. W. and Clark, W. H. 1998. Molecular phylogeny and biogeography of the marine shrimp Penaeus. Mol. Phylogenet. Evol. 10, 399-407.   DOI
5 Bang, I. S. and Lee, Y. S. 2014. Molecular phylogenetic study of Nesiohelix samarangae based on CO-I Gene. Kor. J. Malacol. 30, 391-397.   DOI
6 Boon, Y. W., Taranjeet, K. A. S., Gideon, K. and Han, K. A. O. 2017. Intra- and inter-specific variation of four Acetes Species (Crustacea: Decapoda: Sergestidae) sampled along the west coast of peninsular Malaysia. Sains Malays. 46, 2393-2416.   DOI
7 Espineira, M., González-Lavin, N., Vieites, J. M. and Santaclara, F. J. 2008. Authentication of anglerfish species (Lophius spp) by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and forensically informative nucleotide sequencing (FINS) methodologies. J. Agric. Food Chem. 56, 10594-10599.   DOI
8 Grant, W. S. and Bowen, B. W. 1998. Shallow population in deep evolutionary lineages of marine fishes: Insights from sardines and anchovies and lessons for conservation. J. Heredity. 89, 415-426.   DOI
9 Kang, J. H., Noh, E. S., Park, J. Y., An, C. M., Choi, J. H. and Kim, J. K. 2015. Rapid origin determination of the Northern Mauxia Shrimp (Acetes chinensis) based on allele specific polymerase chain reaction of partial mitochondrial 16S rRNA gene. Asian-Australas. J. Anim. Sci. 28, 568-572.   DOI
10 Jeong, I. J. 2003. Comparative study on the reproductive ecology and population dynamicsof Acetes japonicus and Acetes chinensis on the southwestern coast of Korea. Graduate school of Mokpo National University, Mokpo, Korea.
11 Kang, S. C. 2017. Phylogenetic study of genus Haliotis in Korea by PCR-RFLP analysis. Master thesis, Pukyong National University, Busan, Korea.
12 Kim, S. H., Kim, J. Y., Choi, H. G., Park, J. K. and Min, G. S. 2012. Complete mitochondrial genome of the northern mauxia shrimp Acetes chinensis (Decapoda, Dendrobranchiata, Sergestoidae). Mitochondrial DNA 23, 28-30.   DOI
13 Kim, S. T., Kim, G. R., Yoon, T. H., Kim, C. G., Kim, E. B., Yang, S. J., Baek, S. C., Choi, H. B., Han, K. H., Park, H. and Kim, H. W. 2015. Complete mitochondrial genome of Chinese ditch prawn, Palaemon gravieri (Yu, 1930) (Crustacea: Decapoda: Palaemonidae) in Korean waters. Fish. Aquatic Sci. 10, 289-289.
14 Kress, W. J., Garcia-Robledo, C., Uriarte, M. and Erickson, D. L. 2015. DNA barcodes for ecology, evolution, and conservation. Trends Ecol. Evol. 30, 25-35.   DOI
15 Wilwet, L., Jeyasekaran, G., Shakila, R. J., Sivaraman, B. and Padmavathy, P. 2018. A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNA (val) region to authenticate four commercially important shrimp species in India. Food Chem. 239, 369-376.   DOI
16 Layton, K. K. S., Martel, A. L. and Hebert, P. D. N. 2014. Patterns of DNA barcode variation in canad ian marine molluscs. A9, e95003.   DOI
17 Nadia, H., Imad, N. and Bassam, A. S. 2012. Identification of meat species by PCR-RFLP of the mitochondrial COI gene. Meat Sci. 90. 490-493.   DOI
18 Sivaraman, B., Jeyasekaran, G., Shakila, R. J., Alamelu, V., Wilwet, L., Aanand, S. and Sukumar, D. 2018. PCR-RFLP for authentication of different species of processed snappers using mitochondrial D-loop region by single enzyme. Food Control 90, 58-65.   DOI
19 Slatkin, M. and Hudson, R. R. 1991. Pairwise comparison of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics 129, 555-562.   DOI
20 Sperling, F. A., Anderson, G. S. and Hickey, D. A. 1994. A DNA-based approach to the identification of insect species used for postmortem interval estimation. J. Forensic Sci. 39, 418-27.
21 Wolf, C., Hubner, P. and Luthy, J. 1999. Differentiation of sturgeon species by PCR-RFLP. Food Res. Int. 32, 699-705.   DOI