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Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp. KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea

  • Kim, Ji Hyung;Affan, Abu;Jang, Jiyi;Kang, Mee-Hye;Ko, Ah-Ra;Jeon, Seon-Mi;Oh, Chulhong;Heo, Soo-Jin;Lee, Youn-Ho;Ju, Se-Jong;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.238-246
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    • 2015
  • A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 ± 0.2%, 48.8 ± 1.8%, and 22.2 ± 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.

Identification of vulnerable region susceptible to soil losses by using the relationship between local slope and drainage area in Choyang creek basin, Yanbian China (중국 연변 조양하 유역의 국부경사와 배수면적의 관계를 이용한 토사유실 우심지역 추출)

  • Kim, Joo-Cheol;Cui, Feng Xue;Jung, Kwan Sue
    • Journal of Korea Water Resources Association
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    • v.51 no.3
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    • pp.235-246
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    • 2018
  • The main purpose of this study is to suggest a methodology for identifying vulnerable region in Choyang creek basin susceptible to soil losses based on runoff aggregation structure and energy expenditure pattern of natural river basin within the framework of power law distribution. To this end geomorphologic factors of every point in the basin of interest are extracted by using GIS, which define tractive force and stream power as well as drainage area, and then their complementary cumulative distributions are graphically analyzed through fitting them to power law distribution to identify the sensitive points within the basin susceptible to soil losses with respect to scaling regimes of tractive force and stream power. It is observed that the range of vulnerable region by scaling regime of tractive force is much narrower than by scaling regime of stream power. This result seems to be due to the tractive force is a kind of scale dependent factor which does not follow power law distribution and does not adequately reflect energy expenditure pattern of river basins. Therefore, stream power is preferred to be a more reasonable factor for the evaluation of soil losses. The methodology proposed in this study can be validated by visualizing the path of soil losses, which is generated from hill-slope process characterized by local slope, to the valley through fluvial process characterized by drainage area as well as local slope.

Nurses' Perception on Clinical Nutrition Services by Types of Medical Institution and Area (병원 유형 및 지역에 따른 임상영양서비스에 대한 간호사의 인식)

  • Lee, Han Na;Lee, Song Mi;Park, Yoo Kyung;Lee, Seung Min;Lee, Eun;Cha, Jin A;Park, Mi Sun;Lee, Ho Sun;Rha, Mi Yong;Lyu, Eun Soon
    • Journal of the Korean Dietetic Association
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    • v.20 no.4
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    • pp.235-246
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    • 2014
  • The purpose of this study was to evaluate nurses' perception of clinical nutrition services. A cross-sectional survey design was performed. The research was accomplished by using questionnaires developed for this study and administered from September 12 to December 31, 2013 to 343 nurses at 43 tertiary hospitals and 20 general hospitals. The percentage of nurses who recognized clinical nutrition certificate as issued from nation was 27.8%. The mean scores of perceived usefulness on clinical nutrition services was 4.23/5.00, whereas that of perceived implementation was 3.76/5.00. The mean scores of necessity of disease-specialized clinical dietitian at capital hospitals were significantly higher for obesity (P<0.01), cancer (P<0.05), and infant & childhood disease (P<0.01) than at local hospitals. The rates of nurses' experience in group education on cancer at capital hospitals (21.7%) was significantly higher than that at local hospitals (10.3%) (P<0.05). The mean scores of perceived importance of clinical nutrition services were 4.46/5.00 for 'group nutrition education', 4.46/5.00 for 'individual consultation', and 4.40/5.00 for 'nutrition management for enteral nutrition (EN) patients'. The most common reason why clinical nutrition services are important was 'improving malnutritional status'. To activate clinical nutrition services especially at local hospitals, clinical dietitians should give systematic assistance to patients and also institutional supports are needed.

A Study on Inelastic Lateral-Torsional Buckling of Stepped I-Beams Subjected to Pure Bending (균일모멘트를 받는 계단식 I형보의 비탄성 횡-비틀림 좌굴에 관한 연구)

  • Kim, Jong Min;Kim, Seung Jun;Park, Jong Sup;Kang, Young Jong
    • Journal of Korean Society of Steel Construction
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    • v.20 no.2
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    • pp.237-246
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    • 2008
  • The cross-sections of continuous multi-span beams sometimes suddenly increase, or become stepped, at the interior supports of continuous beams to resist high negative moments. The three-dimensional finite-element program ABAQUS (2006) was used to analytically investigate the inelastic lateral-torsional buckling behavior of stepped beams subjected to pure bending moment and resulted in the development of design equations. The flanges of the smaller cross-section were fixed at 30.48 by 2.54 cm, whereas the width and/or thickness of the flanges of the larger cross-section varied. The web thickness and height of beam was kept at 1.65 cm and 88.9 cm, respectively. The ratios of the flange thickness, flange width, and stepped length of beams are considered analytical parameters. Two groups of 27 cases and 35 cases, respectively, were analyzed for double and single stepped beams. The combined effects of residual stresses and geometrical imperfection on inelastic lateral-torsional buckling of beams are considered. First, the distributions of residual stress of the cross-section is same as shown in Pi, etc (1995), and the initial geometric imperfection of the beam is set by central displacement equal to 0.1% of the unbraced length of beam. The new proposed equations definitely improve current design methods for the inelastic LTB problem and increase efficiency in building and bridge design. The proposed solutions can be easily used to develop new design equation for inelastic LTB resistance of stepped beams subjected to general loading condition such as a concentrated load, a series of concentrated loads or uniformly distributed load.

EFFECT OF CASTING PROCEDURE ON SCREW LOOSENING OF UCLA ABUTMENT IN TWO IMPLANT-ABUTMENT CONNECTION SYSTEMS

  • Ha, Chun-Yeo;Kim, Chang-Whe;Lim, Young-Jun;Kim, Myung-Joo
    • The Journal of Korean Academy of Prosthodontics
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    • v.46 no.3
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    • pp.246-254
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    • 2008
  • STATEMENT OF PROBLEM: The cast abutment has advantages of overcoming angulation problem and esthetic problem. However, when a gold-machined UCLA abutment undergoes casting, the abutment surfaces in contact with the implant may change. PURPOSE: The purpose of this study was to compare the detorque values of prefabricated machined abutments with gold-premachined cast-on UCLA abutments before and after casting in two types of internal implant-abutment connection systems: (1) internal hexagonal joint, (2) internal octagonal joint. Furthermore, the detorque values of two implant-abutment connection systems were compared. MATERIALS AND METHODS: Twenty internal hexagonal implants with an 11-degree taper and twenty internal octagonal implants with an 8-degree taper were acquired. Ten prefabricated titanium abutments and ten gold-premachined UCLA abutments were used for each systems. Each abutment was torqued to 30 N㎝ according to the manufacturer's instructions and detorque value was recorded. The detorque values were measured once more, after casting with gold alloy for UCLA abutment, and preparation for titanium abutments. Group means were calculated and compared using independent t-test and paired t-test (${\alpha}$=0.05). RESULTS: The results were as follows: 1. The detorque values between titanium abutments and UCLA-type abutments showed significant differences in internal octagonal implants (P<0.05), not in internal hexagonal implants (P>0.05). 2. In comparison of internal hexagonal and octagonal implants, the detorque values of titanium abutments had significant differences between two connection systems on the initial analysis (P<0.05), not on the second analysis (P>0.05) and the detorque values of UCLA-type abutments were not significantly different between two connection systems (P>0.05). 3. The detorque values of titanium abutments and UCLA-type abutments decreased significantly on the second analysis than the initial analysis in internal hexagonal implants (P<0.05), not in internal octagonal implants (P>0.05). CONCLUSION: Casting procedures of UCLA-type abutments had no significant effect on screw loosening in internal implant-abutment connection systems, and UCLA-type abutments showed higher detorque values than titanium abutments in internal octagonal implants.

Experimental study on Methods of Fever Control -Effects of ice-pack application on different body areas.- (냉찜질부위에 따른 열 조절 효과에 관한 실험적 연구)

  • 한영자
    • Journal of Korean Academy of Nursing
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    • v.2 no.1
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    • pp.237-246
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    • 1971
  • The purpose of this study is to observe the effects of ice-pack application on different body areas and to note which body area produces the best results according to age, room temperature, sex and disease. Seventeen patients, including six men and eleven women, hospitalized at Seoul National University Hospital from May 22 to August 29, 1971, ranging in age from 15 through 60 years with an oral temperature of 38℃ or over, were chosen for this study. They were observed with regard to the effect of the ice-packs after applications for 30, 45 or 60 minutes. Four ice-packs, 7.6inch by 9 inch, were applied on the chest and the abdominal surface in the control group and the same size and number of packs on the axillae and political regions in the experimental group. The study results were reviewed in a statistical method to obtain the following findings 1. There was no significant difference in the change of the body temperature between the control group and the experimental group after applying the packs for 30, 45 or 60 minutes. 2. Individually, there was a significant difference in the change of body temperature between before and after application for 30, 45 or 60 minutes. 3. The difference of room temperature also had no merle influence on the body temperature taken immediately before applying the ice-packs. Even when ice-packs were applied for 30, 45 or 60 minutes, the room temperature did not influence the reduction of temperature in the patient. 4. There was a significant difference in the change of the body temperature, according to the sex, after application for 30, 45 or 60 minutes. Male patient's body temperature decreased far more noticeably than the female patients and the degree of reduction became more pronounced the longer the packs were applied. 5. In regard to age, in adults, the degree of reduction in the body temperature by the application of ice-packs was similar after appling them for 30, 45 or 60 minutes and did not depend upon the length of application. 6. Regardless of the disease, there was no great difference in the reduction of body temperature when the ice-packs were applied for 30 or 45 minutes, except for patients with typhoid fever when a striking decrease in the fever resulted after a 60 minute application of the ice-pack.

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A Methodology of Decision Making Condition-based Data Modeling for Constructing AI Staff (AI 참모 구축을 위한 의사결심조건의 데이터 모델링 방안)

  • Han, Changhee;Shin, Kyuyong;Choi, Sunghun;Moon, Sangwoo;Lee, Chihoon;Lee, Jong-kwan
    • Journal of Internet Computing and Services
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    • v.21 no.1
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    • pp.237-246
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    • 2020
  • this paper, a data modeling method based on decision-making conditions is proposed for making combat and battlefield management systems to be intelligent, which are also a decision-making support system. A picture of a robot seeing and perceiving like humans and arriving a point it wanted can be understood and be felt in body. However, we can't find an example of implementing a decision-making which is the most important element in human cognitive action. Although the agent arrives at a designated office instead of human, it doesn't support a decision of whether raising the market price is appropriate or doing a counter-attack is smart. After we reviewed a current situation and problem in control & command of military, in order to collect a big data for making a machine staff's advice to be possible, we propose a data modeling prototype based on decision-making conditions as a method to change a current control & command system. In addition, a decision-making tree method is applied as an example of the decision making that the reformed control & command system equipped with the proposed data modeling will do. This paper can contribute in giving us an insight of how a future AI decision-making staff approaches to us.

Free-Radical Polymerization and Copolymerization of N-Acetyl ${\alpha}$-Aminoacrylic Acid (N-Acetyl ${\alpha}$-aminoacrylic Acid의 자유라디칼 중합 및 혼성중합)

  • Il Hyun Park;Chong Kwnag Lee;Jae Ho Choi;Jung-Il Jin
    • Journal of the Korean Chemical Society
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    • v.26 no.4
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    • pp.235-246
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    • 1982
  • The free radical polymerization and copolymerization of N-acetyl ${\alpha}$-aminoacrylic acid were investigated. From the result of kinetic investigation of N-acetyl ${\alpha}$-aminoacrylic acid in DMF at $60^{\circ}C$, a rate equation of $R_p$ = $k_p[M]^{0.97}[I]^{0.59}$ was obtained. The overall activation energy for the polymerization was found to be 25.2 kcal/mole. Copolymerization of N-acetyl ${\alpha}$-aminoacrylic acid with acrylic acid and styrene was carried out for the determination of monomer reactivity ratios. The monomer reactivity ratios for the monomer pairs determined at 70.0{\pm}0.1^{\circ}C$ using benzoyl peroxide as an initiator are; $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.49, $r_2$(acrylic acid) = 1.41, $r_1$(N-acetyl ${\alpha}$-aminoacrylic acid) = 0.44, $r_2$(styrene) = 0.91. The values of Alfrey-Price's Q and e parameters for N-acetyl ${\alpha}$-aminoacrylic acid were calculated to be 0.51 and 0.16 for the both systems. Differential thermal analysis and thermogravimetry showed that acrylic acid copolymers have poorer thermal stability as compared with the homopolymer of N-acetyl ${\alpha}$-aminoacrylic acid.

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A Qualitative Study on Early Childhood Special Education Teachers' Experiences of the Disability Understanding Education in Inclusive Education (통합교육 현장에서 근무하는 유아특수 교사의 장애이해 교육 경험에 관한 질적연구)

  • Kim, Seong Hyun;Lee, Seung Ju
    • Korean Journal of Child Education & Care
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    • v.18 no.2
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    • pp.231-246
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    • 2018
  • The purpose of this study was to investigate early childhood special education teachers' experience, difficulties and need for effective disability understanding education in inclusive education field. The researcher interviewed 3 different early childhood special education teacher. Each participant was individually interviewed 2-3 times with deliberated questions for collecting data. After the interview was analyzed, the contents were categorized into three major themes and twelve sub themes. The three major themes were (1) early childhood special education teachers' difficulties in inclusive education field. (2) experience in the disability understanding education (3) need for proper disability understanding education. First, the teachers were awareness of the difficulties at the inclusive education field due to ambiguity in the role and location, the extension of the expertise, and the misaligned system. Second, teachers stated that the barriers to disability understanding education include creating prejudice, varied according to teachers ' abilities, poor perception of appropriate methods, and low concerns of adults and young children. Third, the results showed that development of the teaching manual, improvement of the administrative system, change in perception of disability understanding education, and disability understanding education based on the experiences of young children were necessary for effective disability understanding education. Based on these results, several specific implications for effective disability understanding education were suggested.

Effect of Long-Term Pyridoxine Depletion on Asparte Aminotransferase and Pyridoxal 5'-Phosphate of Rat Liver Mitochondrial and Cytosolic Fractions (장기간의 Pyridoxine 부족이 쥐 간의 Mitochondria 및 Cytosolic Fraction 에 있는 Aspartate Aminotransferase 및 Pyridoxal 5'- Phoshate에 미치는 영향)

  • 임경숙
    • Journal of Nutrition and Health
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    • v.19 no.4
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    • pp.246-254
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    • 1986
  • Weanling female Sprague Dawley rats were fed d diets containing 22mg pyridoxine. BCI/kg diet (control diet) and l.2mg pyridoxine. BCI/kg diet (deficient diet). One control group and one defi­c dent group were fed their diet throughout growth, g gestation and lactation. After the pups were born and weaned, the deficient group was divided into two groups. One switched to control diet(supple­I mented group) and the other continued the same d deficient diet( deficient group) until 10 week -old. The liver mitochondrial and cytosolic asparate a aminotransferase activity and pyridoxal phosphate content were determined in offspring rats. The aspartate aminotransferase activities in both liver mito$\phi$ondrial and cytosolic fractions of den­d cient group were significantly lower than those of controls, but there were no significant differences between two groups after addition of 1O^{-4}M pyri­d do뼈I phosphate to the medium. By pyridoxine s supplementation after weaning, the reduced aspar­a tate aminotnmsferase activities were only partialy I restored to control levels. The pyridoxal phospha­t te content of deficient group in Iiver mitochondr­ial and cytosoIic fractions were alo significantly different from those of controls, but readily restored by dietary supplementation. These results suggest that there is a quantitative and a qualitative changes of aspartate amino trans­f ferase and pyridoxal phosphate in liver mitochon­d drial and cytosolic fraction by long-term pyrido­x xine deficiency and these reductions can partially recovered by dietary pyridoxine supplementation after weaning.

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