• 한국환경과학회지
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• 제20권8호
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• pp.929-942
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• 2011

A novel conductivity technique was developed to detect penetration depth of the vessel fluid into the feedpipe. For a given reactor geometry, critical agitator speeds were experimentally determined at the onset of feedpipe backmixing using Rushton 6 bladed disk turbine (6BD) and high efficiency axial flow type 3 bladed (HE-3) impellers. The ratio of the feedpipe velocity to the critical agitator speed ($v_f/v_t$) was constant for either laminar or turbulent feedpipe flow regimes. Compared to the results of fast competitive reaction, feedpipe backmixing had to penetrate at least one feedpipe diameter into the feedpipe to significantly influence the yield of the side product. However, higher $v_f/v_t$ than that for L/d = 0 (position at the feedpipe end) of the conductivity technique is recommended to completely eliminate feedpipe backmixing in conservative design criteria. The conductivity technique was successful in all feedpipe flow conditions of laminar, transitional and turbulent flow regimes.

• 대한기계학회논문집B
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• 제27권4호
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• pp.504-510
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• 2003

Simultaneous measurements of turbulent velocity and concentration field in a stirred mixer tank are carried out by using PIV/LIF technique. Instantaneous velocity fields are measured with a 1K$\times$1K CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2002년도 추계학술대회 논문집
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• pp.101-104
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• 2002

Simultaneous measurement of turbulent velocity and concentration field in a stirred mixer tank is carried out by using PIV/LIF technique. Instantaneous velocity fields are measured by a $1K\times1K$ CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• 한국미생물·생명공학회지
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• 제48권3호
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• pp.344-357
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• 2020

Streptococcus zooepidemicus 유래의 세포외 고분자물질인 히알루론산(hyaluronic acid) (HA)을 대량 생산하기 위해, 균주 개량, 생산배지 및 배양공정 개발에 관한 연구를 수행하였다. HA 고생산성 변이주를 선별하기 위해 약 99%의 사멸률을 보이는 ethylmethane sulfonate (EMS) 처리조건을 적용해서, 지속적인 random screening 방법으로 고생산성, 고안정성의 변이주들을 선별할 수 있었다. HA를 고농도로 생산하기 위해서는, 이 균주의 생화학 및 배양생리적 특성에 기반한 최적 배지개발이 필수적이라고 판단하여, one-factor-at-a-time (OFAT), full factorial design (FFD), steepest ascent method (SAM) 및 response surface method (RSM) (반응표면분석법)을 순차적으로 적용하여 통계적 배지 최적화 실험을 수행하였다. 최적 배지조성에서 플라스크 배양에 의한 HA 생산성은 5.38 g/l로서, 이전 배지(3.54 g/l)에 비해 약 52% 향상된 생산량을 얻을 수 있었다. 또한 선별된 우량균주와 최적화된 생산배지를 이용하여 5 L 발효조에서 배양공정 최적화 연구를 수행하였다. 이 균주의 생리학적 특성을 고려할 때, HA 생산성을 높이기 위해서는 (배양 중 HA 축적으로 인해 고점도를 띠는) 배양액으로의 충분한 용존산소 공급이 매우 중요한 요인인 것으로 판단되었다. 따라서 용존산소 공급과 밀접하게 관련있는 발효조의 교반시스템(교반 날개 종류, 크기 및 배치 등) 및 교반속도에 대한 최적화 연구를 수행하였다. 그 결과, 교반축 하부에는 Rushton turbine-type, 상부에는 marine-type의 확장된 교반날개(기존 대비 직경 1.3배 확장)가 설치된 경우, 450 rpm에서 강화된 혼합력과 충분한 용존산소 공급으로 인해 HA 생산성이 기존 플라스크 배양 대비 약 1.8배(9.79 vs. 5.38 g/l) 더 높은 것으로 확인되었다. 최종적으로 HA 배양공정의 scale-up 가능성을 확인하기 위해, pilot 규모의 50 L 발효조 배양을 최대 300 rpm의 교반속도에서 수행하였다. 처음으로 시도한 50 L 배양임에도 불구하고, HA 최대 생산성 면에서 볼 때, 5 L 발효조 결과와 거의 동일한 수준(98.5%) (9.11 vs 9.25 g/l)의 생산량을 얻을 수 있었다. 반면 지수기 성장단계인 배양 15시간까지의 50 L 배양의 HA 평균생산속도(r_p)는 0.46 g/l/hr로서 0.62 g/l/hr인 5 L 배양 대비 약 74% 정도에 머무는 것으로 나타났다. 따라서 생산 발효조의 scale-up 시, 생산균주의 전단응력 민감성(shear damage)을 함께 고려하면서, 산소전달계수(k_La)를 기반으로 하는 교반시스템에 대한 체계적인 연구가 진행된다면, HA 생산속도도 증가될 수 있는 긍정적인 결과를 얻을 수 있을 것으로 기대된다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.1806-1813
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• 2003

The stirred tank reactor is one of the most commonly used devices in industry for achieving mixing and reaction. Here we report on results obtained from the large eddy simulations of flow inside the tank performed using a spectral multi-domain technique. The computations were driven by specifying the impeller-induced flow at the blade tip radius. Stereoscopic PIV measurements (Hill et $al.^{(1)}$) along with the theoretical model of the impeller-induced flow (Yoon et $al.^{(2)}$) were used in defining the impeller-induced flow as superposition of circumferential, jet and tip vortex pair components. Large eddy simulation of flow in a stirred tank was carried out for the three different Reynolds numbers of 4000, 16000 and 64000. The effect of different Reynolds numbers is well observed in both instantaneous and time averaged flow fields. The instantaneous and mean vortex structures are identified by plotting an isosurfaces of swirling strength for all Reynolds numbers. The Reynolds number dependency of the nondimeansional eddy viscosity, resolve scale and subgrid scale dissipations is clearly shown in this study.

• 대한기계학회논문집B
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• 제27권9호
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• pp.1290-1298
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• 2003

The stirred tank reactor is one of the most commonly used devices in industry for achieving mixing and reaction. Here we report on results obtained from the large eddy simulations of flow inside the tank performed using a spectral multi-domain technique. The computations were driven by specifying the impeller-induced flow at the blade tip radius. Stereoscopic PlY measurements (Hill et al. $^{(1)}$) along with the theoretical model of the impeller-induced flow (Yoon et al. $^{(2)}$) were used in defining the impeller-induced flow as superposition of circumferential, jet and tip vortex pair components. Large eddy simulation of flow in a stirred tank was carried out for the three different Reynolds numbers of 4000, 16000 and 64000. The effect of different Reynolds numbers is well observed in both instantaneous and time averaged flow fields. The instantaneous and mean vortex structures are identified by plotting an isosurfaces of swirling strength for all Reynolds numbers. The Reynolds number dependency of the non-dimensional eddy viscosity, resolved scale and subgrid scale dissipations is clearly shown in this study.