• 한국산업보건학회지
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• 제20권2호
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• pp.79-87
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• 2010

Objectives: The purpose of this study was to investigate the symptoms of work-related musculoskeletal disorders (WMSDs), the influence of the WMSDs on the health-related quality of life (HRQoL), and to identify the differences of WMSDs and HRQoL between greenhouse farmers and paddy farmers. Methods: Subjects were 34 greenhouse farmers and 56 paddy farmers who were living in a rural community. The sociodemographic characteristics, health-related behavior, working characteristics, WMSDs and HRQoL were investigated. WMSDs symptoms were measured by a selfassessed questionnaire on musculoskeletal disorders symptoms and HRQoL was measured by the Euro Quality of Life (Euroqol) EQ-5D index. The differences on the WMSDs, EQ-5D and it's related factors between the groups were assessed with t-test and ${\chi}^2$-test. Results: Greenhouse farmers were longer the working hours per day and working duration than paddy farmers. The level of prevalence of the WMSDs in greenhouse farmers is higher than in paddy farmers. The EQ-5D index of greenhouse farmers and paddy farmers were 0.819 and 0.874, respectively. Overall, the mean value of EQ-5D index was significantly lower greenhouse farmers than paddy farmers. Conclusions: These results showed that the HRQoL for farmers are strongly influenced by WMSDs and agicultural type in that we are aware of the need to comply with regulations of WMSDs, especially greenhouse farmers.

• The Plant Pathology Journal
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• 제22권1호
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• pp.21-27
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• 2006

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

• The Plant Pathology Journal
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• 제25권1호
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• pp.95-98
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• 2009

The incidence and occurrence of virus infecting paprika (Capsicum annuum var. grossum) in Jeonnam province, the main areas of cultivation in Korea is undetermined. In this study, a total of 1,020 samples with virus-like symptoms were collected in Jeonnam province during summer season for 3 consecutive years (2002-2005) and were tested using enzyme linked immunosorbent assay (ELISA). Results showed that Pepper mottle virus (PepMoV), Broad bean wilt virus (BBWV), and Cucumber mosaic virus (CMV) were found to be the most prevalent viruses with a 3-year average percent incidence of 41.3, 19.8, and 4.4 respectively. Mixed infection with more than two viruses was also found with 3.5%, 17.0%, and 8.3%, respectively. Symptoms of these virus diseases were not evident at the seedling stage but slowly appeared at the transplanting stage and increased to the middle stage (4-5 months after transplanting) during the 3-year cultivation periods. Symptom appearance of infected plants however varied largely with transplanting time. Those plants transplanted from November to January were found to be infected with viruses in June, whereas symptoms appeared with in a month those plants transplanted from June to August. There were differences in the virus incidence from primary factor such as district, type of green house and variety, but these were not statistically significant (data not shown). Recommended control measures of paprika against these viruses is also discussed in this paper.

• The Plant Pathology Journal
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• 제33권5호
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• pp.458-466
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• 2017

Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.

• Journal of Preventive Medicine and Public Health
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• 제33권3호
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• pp.271-279
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• 2000

Objectives : To assess the seroprevalence and risk factors of toxoplasmosis in high school students in Cheju Province, Korea. Methods : A total of 4,570 high school students from 18 schools in Cheju Province were investigated for Toxoplasma gondii antibodies(IgG) by enzyme linked immunosorbent assay (ELISA). Risk factors for toxoplasmosis, such as place of residence, type of house, contact with cats and other pets, and rare meat consumption, were examined by questionnaire. Results . The overall antibody positive rate was 5.5% and ranged from 2.6 to 11.5% by school. There was no significant difference between males and females. Statistical analyses of the questionnaire data indicated that the risk factors for seropositivity were: (1) birth place (Cheju/others), (2) place of residence (rural/urban), (3) dietary habits (vegetarian/non vegetarian), (4) eating rare meat, (5) exposure to pets and (6) hepatitis B. Conclusion : We confirmed that the prevalence of the anti-Toxoplasma gondii antibody in a population of high school students in Cheju Province was to the previously reported prevalence.

• 한국임상수의학회지
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• 제8권2호
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• pp.153-156
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• 1991

경기도의 19부락에서 1989년 5월에 459두와 그해 11월에 446두의 소를 대상으로 하여 렙토스피라 감염유무를 조사하였다. 혈액배양에서는 포모나 항체에 강하게 반응하는 한 균주만이 검출되었다. 몇개의 표준균주를 사용한 혈청검사에서는 5월에 12%, 11월에 26%의 양성율을 나타내었다. 년령이 증가함에 따라 양성율이 높아졌으며 숫소가 암소보다 높았다. 혈청검사 양성과 소의 유산력과는 유의적인 관계가 인정되지 않았다.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3599-3609
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• 2010

연안개발과 해안매립을 사용하여 개발된 새로운 도시 건설의 수요가 증가하면서 해안구조물과 고층구조물의 시공이 증가하는 추세이다. 이러한 구조물에서 수평력과 인발력을 받는 구조물의 기초의 적용이 증가하고 있으며, 특히 구조물에서 인발력에 효과적으로 저항하기 위한 기초형식으로 선단확장형말뚝의 사용이 증가하고 있는 실정이다. 압축력을 받는 기초에 관한 많은 연구들이 이루어지고 있으나, 선단확장형말뚝의 인발저항에 관한 연구는 미비한 상태이다. 따라서 본 논문에서는 국외 4개 현장을 대상으로 선단확장형말뚝을 시공하고 정재하시험을 실시하여 말뚝의 지지력 및 하중-변위특성과 주면마찰력을 고려한 하중분담을 고찰하였다. 또한 3차원 유한요소해석 및 이론에 의해 산정된 극한인발력과 현장시험의 극한인발력을 비교 평가하였다.

• 한국식품조리과학회지
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• 제11권4호
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• pp.370-378
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• 1995

This study is to seek methods for optimum management of foodservice system for elementary schools in Korea through surveying and analyzing the current status of the "common-management foodservice system" and certain important factors relating to its operations. For the survey of the current status of the "common-management foodservice system", questionnaires are prepared as against schools adopting "common-management system"("Common Schools") as well as schools adopting "conventional management system" ("Conventional Schools") in Kyungsangbookdo ("Kyungbook") area. The survey items are composed of general items, items concerning tasks of dietitians, items concerning purchasing activities and items concerning level of recognition and job-satisfaction of the dietitians. Total 46 dietitians from Common Schools and 65 dietitians from Conventional Schools in Kyungbook region responded the questionnaires. The results of this study are as follows: 1. Information Concerning Dietitians Average age of the dietitians: 26.6 yrs Ratio of two years or less of experiences as school dietitian: Dietitians from Common Schools: 59.1％ Dietitians from Conventional Schools: 50.8％ 2. Types of the Foodservice In both schools, rural type are prevalent; however, between urban types and islands types, the ratio of urban types are larger than the ratio of islands types in Conventional Schools, and the opposite is true in Common Schools. 3. Decision of Suppliers The ratio of dietitian＇s participaion in the decision making reaches only 38.3％, indicating the possibility of problems in quality control and hygienic aspects of the food supply. 4. Factor Analysis of the Foodservice Duties Food supply and general affairs administration work was carried out very well (4.2), while personnel management and operational improvement work (2.8), nutritional education work (2.3), and information related work were poorly carried out. No significant differences were found between the two types of the foodservice management systems. 5. Job Satisfaction scores of Dietitians Dietitians were more satisfied with the works (50.0), supervision (50.5) and co-workers (46.9) than pay (18.0) and promotion (22.3).

• 한국환경복원기술학회지
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• 제9권2호
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• pp.1-15
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• 2006

As the imports of foreign agricultural products are liberalized and the consumption of agricultural products declines, abandoned rice paddy fields continues to rise. However, such abandoned rice paddy fields has not been precisely surveyed yet. In this backdrop, a necessity to develop technology to utilize such abandoned rice paddy fields has emerged. Utilization of abandoned rice paddy fields as wetlands may be a good example. This study aimed to survey the current status and characteristics of wetlands created within abandoned rice paddy fields by selecting abandoned rice paddy fields throughout the nation and conducting field surveys on the sites that had transformed into wetlands. The abandoned rice paddy fields almost transformed into wetland and the types of wetlands transformed from abandoned rice paddy fields were mainly Inland/Moutain/Depression/Abandoned rice paddy fields/Marsh/Phragmites communis community and Inland/Moutain/Depression/Abandoned rice paddy fields/Swamp/Salix koreensis community. Abandoned rice paddy fields that had transformed into wetlands was depending heavily on waterways for water supply than other reservoirs and lakes do. Abandoned rice paddy fields transformed into wetlands was most observed in mountainous area. Abandoned rice paddy fields are because agricultural land is no longer profitable due to international and social changes and is not cultivated as government policy. Wetland period and dimension originated from abandoned rice paddy fields are very various and its surrounding land its mostly forest and the next largest follow roads and rural community. The abandoned rice paddy fields transformed into wetlands is mostly deserted currently. Despite their value as wetlands, no restoration and utilization efforts are made in Korea today. Therefore, it is imperative to conduct a precise current status survey on these areas and introduce management and restoration plans at the government level in the case of important habitats.

• Journal of Biosystems Engineering
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• 제35권4호
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• pp.215-223
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• 2010

In order to develop an precision aerial pesticide application system to be attached to an unmanned helicopter which can be applied to small lots of land, this study analyzed the flowing and spraying characteristics of the spray droplets by the main rotor downwash by setting the application conditions at the flight altitude of 3 m, the diameter of main rotor of 3.1 m, the boom length of around 2.8 m, and the spraying rate of 8 L/ha. The results of this study are summarized below. Through analysis of the covering area ratio of the spray droplets by main rotor downwash by nozzle type, boom with tilt angle and height, it was found that the covering area ratio of the twin flat-fan nozzle of around 25% was more uniform than other types of nozzle, also boom with $10^{\circ}$ tilt angle and spraying height of 3 m was shown to be the appropriate conditions for aerial application of pesticides. It was found that the nozzle position to minimize the scattering loss of spray droplets due to vortex phenomenon at both ends of the main rotor was around 10 cm from the end of the main rotor. An application test for the aerial pesticide application system attached to the HUA-ACEI unmanned helicopter developed by the Rural Development Administration showed that the range of covering area ratio of the spray droplets was 10-25%, and the spraying width was approximately 7 m when over 10% of covering area ratio was considered for valid spraying.