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Influence of basal medium formulations and silver nanoparticles on in vitro plant growth in gerbera

  • Hyun Hee Kang;Aung Htay Naing;Junping Xu;Mi Young Chung;Su Young Lee;Jeung-Sul Han;Chang Kil Kim
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.183-189
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    • 2023
  • This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

The current Status and Utilization of technology laboratory at the junior high school in Chungbuk Province in Korea (충청북도 중학교 기술실 현황과 활용 실태)

  • Kim, Nan Hui;Yi, Sang Bong
    • 대한공업교육학회지
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    • v.38 no.1
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    • pp.125-143
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    • 2013
  • The purpose of this study was to investigate the current Status and practical use of technology laboratory at the junior high school in Chungbuk Province in order to give some suggestions on hands-on activities for technology education. First, concerning present actual conditions of technology laboratory, There was a large disparity between the urban and rural schools in the possession of a technology laboratory, as the rate of the schools equipped with the rooms respectively stood at 69.84 percent and 33.38 percent in the urban and rural regions. By the type of foundation, every national school, 86.24 percent of the public schools and 37.50 percent of the private schools were equipped with the technology laboratory. By school size, 35.59 of the schools with fewer than 10 classes, 62.11 percent of the schools with 10 to fewer than 30 classes and 85.71 percent of the schools with 31 classes or more were in possession of the laboratory. Thus, the type of foundation and school size made differences to that. As a result of asking the schools without the laboratory about the reason, as many as 88.52 percent had been equipped with the laboratory in the past but converted them into spaces for another purpose. When the schools that had no such laboratory were asked a question whether they had any plans to install a technology laboratory, just five schools(8.19%) had that plan. Second, as for the practical use of the technology laboratory, for what the rooms were actually used was asked, and most of the schools made use of them as Comprehensive General laboratory. As to the size of the rooms, each of the rooms was as large as a classroom($66m^2$) in 62.12 percent of the schools, and their region, type of foundation and student gender made little significant differences to that. Regarding the time for utilizing the laboratory, the majority of the schools used the laboratory approximately once or twice a year, and their region, type of foundation, student gender and school size made few distinctive differences to that. In terms of budget for practice in the rooms, the largest number of the schools that accounted for 36.36 percent earmarked three thousand won to less than five thousand won per student.

Comparative Analysis of Satisfaction according to Opened-Fencing in Campus Afforestation Project Types - Focused on University in Seoul - (대학교 담장개방 녹화사업 유형에 따른 이용 만족도 비교 분석 - 서울 소재 대학 캠퍼스를 중심으로 -)

  • Lee, Se-Mi;Kim, Dong-Chan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.6
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    • pp.57-66
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    • 2011
  • This study researched those universities for which fence opening and greening projects are being conducted by Seoul city. The forms of opened fences at 24 universities which have accomplished this project were classified into several types for each type of university, representative cases with many diverse facilities and active users were selected and investigated. The study was carried out using methods of field observations, literature review, and surveys. To maintain the confidentiality of the collected questionnaire analysis, the analysis of each type's usage frequency, overall satisfaction and a regression analysis with space environment and facilities, a one-way ANOVA for was used to validate the difference between types regarding satisfaction with the project. The results of usage type analysis were found to agree with the 3 analysis criteria-- installation location, user characteristics, and usage purpose--which were the legislative concepts. In overall satisfaction with facilities, it appeared that except for Seoul Women's College of Nursing with its rural district neighborhood type park, users were satisfied: with the small urban neighborhood park of Methodist Theological College, Konkuk University's small urban square park, and Sejong University's green space small city park. In general, users appeared to not have satisfaction with such features as fountains / hydroponic facilities, fitness facilities, and square facilities, which should be taken into consideration when pursuing further opening and greening projects. Regarding full satisfaction with the space environment, it was found that users were not satisfied with Seoul Women's College of Nursing's rural district neighborhood-style park, whereas they were satisfied with Methodist Theological College's small urban neighborhood park, Konkuk University's small urban square-style park, and Sejong University's green space small city park. In addition, it was shown that facilities use, convenience and privacy of the four parks were largely unsatisfactory for users, and that the small city parks located at roadsides were unsatisfactory regarding noise level, both of which should be most highly considered when conducting similar projects in the future.

Behaviors Related to Pornography in Primary, Middle and High School Students (초.중.고등학생의 음란물 접촉 일부 행태)

  • Kim, Hyeon-Ok;Park, Gwang-Sug
    • Child Health Nursing Research
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    • v.14 no.1
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    • pp.90-98
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    • 2008
  • Purpose: This study was done to investigate behavior related to viewing pornography sites by primary, middle, and high school students in one province. Methods: The participants were 2,939 primary (5, 6 grade), middle and high school students (1, 2 and 3 grade), chosen at random in big cities, small towns and rural areas of North Cholla Province. Results: The first experience of finding pornography sites by oneself was 21.1% for the total sample and for primary school students 6.8%, for middle school students, 31.0%, and for high school students, 62.2%. The first time on a pornography site was highest for middle school students (62.0%) and for grade, middle school 1 grade (25.7%). The pornography use resulted in difficulties with study (11.3%), desire to imitate what was seen in pornography (10.9%), imitation of what was seen the pornography (3.9%), and desire to visit pornography sites everyday (2.3%). Pornography contact behavior was related to gender and school type (p<.05). Independent access to pornography and experiencing difficulties with study were related to area of residence (p<.05). Conclusion: These findings suggest that systematic education policy and further research on needs for pornography contact should be examined to develop programs to decrease pornography contact behavior.

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Potential Biotypes in Korean Isolates of Bipolaris cactivora Associated with Stem Rot of Cactus

  • Kim, Jeong-Ho;Jeoung, Myoung-Il;Hyun, Ick-Hwa;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.20 no.3
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    • pp.165-171
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    • 2004
  • A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7% and 95.1%, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.

A Study on the Working Poor among Korean Echo Boomers (에코부머의 근로빈곤에 관한 연구)

  • Park, Mee Sok;Park, Mee Ryeo
    • Human Ecology Research
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    • v.52 no.1
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    • pp.87-101
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    • 2014
  • The purpose of this study is to identify the current status of the working poor and the factors that are influential to the working poor among echo boomers. This study is a secondary analysis using the data from the 7th analysis of the 2012 Korea Welfare Panel Survey, conducted by Korea Institute for Health and Social Affairs. This analysis considered Korean echo boomers born between 1979 and 1985. In our final analysis, we used 1,226 echo boomers who had the ability to work. For the analysis, the SAS/PC statistical program ver. 9.3 was utilized. The research findings are as follows; Firstly, of the working poor among the echo boomers, only 22% were in the state of poverty. Secondly, more echo boomers living in a metropolitan city, city, gun/urban-rural area and district than those in the Seoul were categorized as among the working poor; the same was true for those who lived in a free house than in a leased room or a room that was rented by the month. Thirdly, more echo boomers whose education level was high school graduate or less rather than college graduate or less or university graduate or more were categorized as among the working poor; this was also true for echo boomers whose employment type was temporary employment, self-employment, or unemployment rather than a full-time job.

The Roles and Challenges of Agricultural Extension Program Evaluation (농촌지도사업 프로그램 평가와 농촌지도사의 역할)

  • Park, Duk-Byeong
    • Journal of Agricultural Extension & Community Development
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    • v.10 no.1
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    • pp.43-56
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    • 2003
  • Evaluation in both an art and a science. The art of evaluation involves working with the management to agree upon purpose and users of results, creating design and gathering information that are appropriate for a specific situation and a particular policy making context. The value of evaluating extension p개grams has received a lot of attentions recently, and many extension educations see evaluation as an integral part of their work. The science of evaluation involves determining standards and developing indicatiors, selecting methods appropriate to gather information in a systematic way, analyzing information to assist in determining the value of the program in an objective manner. First, extension specialists have to consider relative merits about methods of gathering evaluation data. Selection of method should be influenced by the type of information desired, time availability, and cost of using the method. Second, good evaluations involve stakeholders at all stages including planning, implementation, and utilization of results. Third, far from being an "add-on ," evaluation begins with the initial planning of an educational program. Fourth, it is important for extension specialists that although evaluation is valuable and essential in any effective program, one of the biggest mistakes of extension program evaluators in to promise results that cannot possibly solve all the problems of project.

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Physicochemical characteristics and antioxidant potential of paprika (Capsicum annuum L.) wine

  • Kim, Chan Yong;Kwon, Oh Hun;Gun, Won Jong;Park, Yong-Sung;Dhungana, Sanjeev Kumar;Kim, Il-Doo
    • Korean Journal of Food Science and Technology
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    • v.51 no.6
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    • pp.592-595
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    • 2019
  • Paprika (Capsicum annuum L.) contains various phytochemicals, including carotenoids, ascorbic acid, tocopherol, flavonoids, and phenolic compounds, as well as natural food colorants. Very little information is available regarding wine produced from different colored paprikas. The objectives of this study were to prepare wines from red, orange, and yellow paprika and evaluate their physicochemical characteristics. The alcohol concentration, pH, titratable acidity, and reducing sugar content were not significantly affected by the type of paprika. Hunter's color values varied with the color of paprika. The total mineral content and 2,2-diphenyl-1-picrylhydrazyl radical scavenging potential of red paprika wine were significantly higher; however, the total polyphenol content of yellow paprika wine was significantly higher than that of the other wine samples. This study suggested that paprika could be used to prepare wine and red paprika might be appropriate for producing good-quality wine.

Effect of extraction temperature on physicochemical constituents and antioxidant potentials of Pu-erh tea

  • Choi, Sung-Hee;Kim, Il-Doo;Dhungana, Sanjeev Kumar;Park, Kwangsoo
    • Korean Journal of Food Science and Technology
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    • v.51 no.6
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    • pp.584-591
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    • 2019
  • Of all the various types of tea that are available, Pu-erh tea has attracted much attention because of its healthpromoting effects. The objective of this study was to investigate the physicochemical and antioxidant properties of two types of Pu-erh tea, Gushu Pu-erh and typical Pu-erh, extracted at 80 and 100℃, respectively. The Gushu Pu-erh extracts showed lower pH but higher antioxidant potential at both the temperatures, as compared to the typical Pu-erh. The mineral content of the tea extracts was greater at 100℃ than at 80℃. Among the seven categories of the flavor compounds that were detected, the peak area of esters was the highest in the Gushu Pu-erh extracts. The type of tea and the extraction temperature significantly affected the physicochemical and functional properties of these tea extracts. This study revealed that Gushu Pu-erh extracted at 100℃ for 3 min with 30 s of shaking provides better results in terms of the antioxidant potential and mineral content.

Expression of Arabidiopsis CAX4 in tomato fruits increases calcium level with no accumulation of other metallic cations

  • Jeong, Se-Woon;Han, Jeung-Sul;Kim, Kyung-Min;Oh, Jung-Youl;Kim, Byung-Oh;Kim, Chang-Kil;Chung, Jae-Dong
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.337-343
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    • 2008
  • We generated transgenic tomato plants with Arabidopsis thaliana $H^+$/cation exchanger gene (C4X4) by Agrobactrium-mediated transformation. We confirmed transgene copy number and transcription by Southern and Northern blot analyses. The intact CAX4-expressing tomato (Lycopersicon esculentum) fruits contained 63-71% more calcium ($Ca^{2+}$) than wild-type fruits. Moreover, ectopic expression of C4X4 in tomato fruits did not show any significant increase of the four kinds of metallic cations analyzed ($Mg^{2+}$, $Fe^{2+}$, $Mn^{2+}$, and $Cu^{2+})$. The C4X4-expressing tomato plants including their fruits did not show any morphological alternations during whole growth period. These results suggest the enhanced Ca-substrate specificity of CAX4 exchanger in tomato. Therefore, intact CAX4 exchanger can be a useful tool for $Ca^{2+}$ nutrient enrichment of tomato fruits with reduced accumulation of undesirable cations.