• Journal of the Korean Institute of Rural Architecture
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• v.21 no.4
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• pp.1-8
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• 2019

This study organize the concept of sustainable development by compiling New-urbanism, sustainable urban regeneration and theory of development. And the framework of analysis about the planning characteristics of New Urbanistic Waterfront and urban development was derived by compiling theory of Waterfront and Waterfront city and considering prior study. This study will analyze the advanced cases at home and abroad through the analysis of the urban development and architectural characteristics analysis system of the New Urbanism Waterfront and Waterfront City Development by analyzing the advanced cases of the domestic and foreign countries through the analysis.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.209-213
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• 2008

This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 $mg{\cdot}L^{-1}$ thidiazuron(TDZ) and 0.5 $mg{\cdot}L^{-1}$ indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.

• Journal of Mushroom
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• v.12 no.2
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• pp.77-81
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• 2014

Mushrooms are considered not only as food but also for source of physiologically beneficial medicines. The culinary-medicinal mushrooms may important role in the prevention of age-associated neurological dysfunctions, including Alzheimer's and Parkinson's diseases. Hericium erinaceus (H. erinaceus), is edible mushrooms, is a parasitic fungus that grows hanging off of logs and trees and well established candidate for brain and nerve health. H. erinaceus contains high amounts of antioxidants, beta-glucan, polysaccharides and a potent catalyst for brain tissue regeneration and helps to improve memory and cognitive functions. Its fruiting bodies and the fungal mycelia exhibit various pharmacological activities, including the enhancement of the immune system, antitumor, hypoglycemic and anti-aging properties. H. erinaceus stimulates the synthesis of Nerve Growth Factor (NGF) which is the primary protein nutrient responsible for enhancing and repairing neurological disorders. Especially hericenones and erinacines isolated from its fruitin body stimulate NGF, synthesis. This fungus is also utilized to regulate blood levels of glucose, triglycerides and cholesterol. H. erinaceus can be considered as useful therapeutic agents in the management and/or treatment of neurodegeneration diseases. However, this review focuses on in vitro, in vivo and clinical trials for neurodegerative disease.

• Journal of the Korean Institute of Rural Architecture
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• v.20 no.3
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• pp.19-26
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• 2018

There are still a number of "olles" existing with traditional alley characteristics in the inner city of Jeju. As a matter of fact, the renovation of old buildings and the protection of alley space are facing a dilemma due to the existence of less than 4m-width-roads in the area of "Mu-geun-sung". This study examines the current status of alley space in "Mu-geun-sung" area; and puts forward to the potential possibility and limitation of alley space regeneration via exploring the applicable scheme of building agreement. In the field survey, a number of regions in inner city of Jeju witnesses the existing majority of invaluable, conventional olles. So as to grasp the feasibility in "Mu-geun-sung" area, we designs a simulating experiment to carry out the Building Agreement; and compares with the current development model in this area. Building Agreement implies to establish gradually a separation of land ownership under the existing unified development of landowners and users, thus to explore a variety of land exploitation systems by separating land ownership and right to use land. As a result, the deterioration of the street landscape results from the increase of building storey numbers. The damages to the surrounding environment brought by the newly built buildings will be minimized as well as keeping the maximum building volume rate. Obviously, these measures will alleviate infringement to the traditional residential sunlight and privacy space effectively, and reduce the pressure from high-rise buildings to the alley space, so that the landscape of the alley will enhance to form a more comfortable alley environment.

• The Korean Journal of Mycology
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• v.35 no.2
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• pp.81-84
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• 2007

In some cases, the problem with the mycelium of Tricholoma giganteum is delayed mycelial growth or non-regeneration. Therefore, this study was conducted to understand the mycelial storage condition of T. giganteum and to investigate the regeneration ratio of mycelial growth. T. giganteum obtained from KACC in RDA was evaluated for its simple preservation at $10^{\circ}C$ and subcultured on different media. Mycelium of T. giganteum was cultured on the PBA (potato bamboo extract medium) for seven days at $30^{\circ}C$. Using simple preservation method, the mycelium of T. giganteum (MKACC 50852) and Pleurotus ostreatus (Chunchu No. 2) were stored on six different media in two kinds of storage vessels (tube and vial) for a period of 1-12 months at $4^{\circ}C,\;15^{\circ}C$, and $25^{\circ}C$ storage temperatures. At $4^{\circ}C$ storage condition, mycelial regeneration was failed in all agar media, but was successful in the sawdust medium for 3 months. At $15^{\circ}C$ storage, mycelial activity was maintained up to six months. On the other hand, P. ostreatus produced well-regenerated mycelia both at $4^{\circ}C$ and $15^{\circ}C$ storage up to 12 months. In conclusion, it is estimated that the mycelia storage condition of T. giganteum must be done at $15^{\circ}C$ and subcultured within six months.

• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.233-245
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• 2013

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an important source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differentiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphorylation, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

• BMB Reports
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• v.45 no.12
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• pp.742-747
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• 2012

Granulocyte colony-stimulating factor (G-CSF) is used for heart failure therapy and promotes myocardial regeneration by inducing mobilization of bone marrow stem cells to the injured heart after myocardial infarction; however, this treatment has one weakness in that its biological effect is transient. In our previous report, we generated 5 mutants harboring N-linked glycosylation to improve its antiapoptotic activities. Among them, one mutant (Phe140Asn) had higher cell viability than wild-type hG-CSF in rat cardiomyocytes, even after treatment with an apoptotic agent ($H_2O_2$). Cells treated with this mutant significantly upregulated the antiapoptotic proteins, and experienced reductions in caspase 3 activity and PARP cleavage. Moreover, the total number of apoptotic cells was dramatically lower in cultures treated with mutant hG-CSF. Taken together, these results suggest that the addition of an N-linked glycosylation was successful in improving the antiapoptotic activity of hG-CSF, and that this mutated product will be a feasible therapy for patients who have experienced heart failure.

• Korean Association of Arts Management
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• no.56
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• pp.275-304
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• 2020

This paper discusses a way to differentiate contents and enhance the competitiveness of art spaces in rural regions through place assets, noting the growing number of rural art spaces and their great potential as cultural spaces in recent years. First, we surveyed existing research and resources to understand the current status and roles of rural art spaces in the context of theoretical discussions on place assets. Based on this, we classified place assets into physical, human, and symbolic elements. After that, we conducted two case studies that apply this classification. For the case studies on the Ami Art Museum and the Potato Flower Studio, we investigated the sites and conducted in-depth interviews with the directors following a qualitative research method. Our findings through the case studies show that Ami Art Museum and Potato Flower Studio remodeled closed school buildings, which are known as physical place assets in rural areas. However, there was a difference in utilizing other place asset elements, and this resulted in different types of differentiated contents. We found three types of rural art spaces from two case studies: inhabitant-friendly, artist-friendly, and tourist-friendly. Finally, in our research, we presented a method of utilizing place assets that differentiate rural art places along with a method for developing differentiated contents. To conclude, the significance of our study is that it attempted to find out a way to make rural art spaces sustainable, and sought cultural regeneration in rural areas through revitalization of rural art spaces.

• Journal of the Korean Regional Science Association
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• v.38 no.2
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• pp.21-41
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• 2022

The purpose of this study is to compare the differences in the subjective quality of life (QoL) perceived by residents in different settlement spaces such as urban vs. rural and Seoul Capital Area (SCA) vs. Non-Capital Area (NCA). Using the data of the 'Quality of Life Satisfaction Survey for Balanced Development' conducted nationwide by the Presidential Committee for Balanced National Development in 2018, a propensity score matching method was introduced to compare the differences between the types of settlement spaces. The results show that no significant difference is found between all the types in satisfaction with local life, and in the case of happiness, rural residents show a higher level than those living in cities. Looking at the quality of life by QoL domain, the QoL of urban residents in SCA is generally the highest in the basic living environment, medical care, and culture/leisure domains, while the QoL of rural residents in the job/income, environment, community, and welfare domains is higher than that of residents of SCA and NCA cities. Interestingly, in no domains NCA cities show their relative strength. These results show the value of rural areas in the face of increasingly prevailing urbanization trends, and suggests that the value of rural areas must be strengthened for rural development. It is necessary to pursue policy efforts such as rural regeneration based on spatial planning in order to respond to the disorganized development that damages the value of the rural environment and landscape and to harmoniously maintain and develop the rural settlement.

• Plant Resources
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• v.8 no.2
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• pp.81-86
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• 2005

An efficient plant regeneration system of Gentiana scabra through somatic embryogenesis was established. Leaves and roots of seedlings of Gentiana scabra excised after germination were cultured on MS basal medium with 2,4-D, NAA or BA. Embryogenic callus was obtained on MS medium with 0.5 mg/L 2,4-D alone or 0.1 mg/L 2,4-D combimation with 1.0 mg/L BA after 45 days of culture. These embryogenic calli gave rise to somatic embryos, which subsequently developed into plantlets on MS medium without PGRs. Also, shoots were effectively differentiated from embryogenic callus when root segments were cultured on MS medium supplement with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Shoots were effectively rooted on MS medium without PGRs. In vitro flowers were formed from plantlets cultured on MS medium with $5\%$ sucrose after 60 days of culture.