• Journal of Ginseng Research
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• 제42권1호
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• pp.107-115
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• 2018

Background: Depression is one of the most commonly diagnosed neuropsychiatric diseases, but the underlying mechanism and medicine are not well-known. Although Panax ginseng has been reported to exert protective effects in various neurological studies, little information is available regarding its antidepressant effects. Methods: Here, we examined the antidepressant effect and underlying mechanism of P. ginseng extract (PGE) in a chronic restraint stress (CRS)-induced depression model in mice. Results: Oral administration of PGE for 14 d decreased immobility (depression-like behaviors) time in forced swim and tail suspended tests after CRS induction, which corresponded with attenuation of the levels of serum adrenocorticotropic hormone and corticosterone, as well as attenuated c-Fos expression in the amygdala. PGE enhanced messenger RNA expression level of brain-derived neurotrophic factor but ameliorated microglial activation and neuroinflammation (the level of messenger RNA and protein expression of cyclooxygenase-2 and inducible nitric oxide synthase) in the amygdala of mice after CRS induction. Interestingly, 14-d treatment with celecoxib, a selective cyclooxygenase-2 inhibitor, and $N_{\omega}$-nitro-L-arginine methyl ester hydrochloride, a selective inducible nitric oxide synthase inhibitor, attenuated depression-like behaviors after CRS induction. Additionally, PGE inhibited the upregulation of the nuclear factor erythroid 2 related factor 2 and heme oxygenase-1 pathways. Conclusion: Taken together, our findings suggest that PGE exerts antidepressant-like effect of CRS-induced depression by antineuroinflammatory and antioxidant (nuclear factor erythroid 2 related factor 2/heme oxygenase-1 activation) activities by inhibiting the hypothalamo-pituitary-adrenal axis mechanism. Further studies are needed to evaluate the potential of components of P. ginseng as an alternative treatment of depression, including clinical trial evaluation.

• Journal of Crop Science and Biotechnology
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• 제10권1호
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• pp.50-56
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• 2007

Barley S-adenosylmethionine synthetase1 gene, which was differentially expressed in seed development of extra early barley, was regulated by the phytohormones and abiotic stresses. In order to identify the regulation regions which were involved in transcriptional control of the phytohormones and abiotic stresses, we isolated 1459 bp fragment of HvSAMS1 gene promoter using genome walking strategy and deletion series were constructed. Deleted upstream fragments(-1459, -1223, -999, -766, -545, -301 bp) were fused to the GUS reporter gene and evaluated via Agrobacterium-mediated transient expression assay. Increased GUS activity of HvSMAS1 promoter -301/GUS construct under each of NaCl, $GA_3$, ABA and ethylene application was found. However, GUS activity was negligible in the leaves transformed with the HvSMAS1 promoter(-1459, -1223, -999, -766 and -545)/GUS constructs. No significant induction of GUS activity was observed for the ethionine and spermidine treatments. In order to locate promoter sequence of the HvSAMS1 gene that was critical for the activation of gene expression, deletion and addition promoter derivatives(+, includes 43 bp of 5' ORF) of the HvSAMS1 gene fused to the GUS reporter gene were applied. The tobacco leaves which harbored the additional HvSAMS1 promoter(-1459+, -1459 to -546, -545+ and -301+)/GUS construct did not significantly induce GUS activity as compared to the HvSAMS1 promoter(-1459, -545 and -301)/GUS constructs under each of NaCl, ABA and $GA_3$ treatment. However, the GUS activity was high in the tobacco leaves which harboring the -211 to -141 regions of the HvSAMS1 promoter. This result suggested that HvSAMS1 gene expression might be regulated by this region(from -211 to -141).

• 벤처창업연구
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• 제13권4호
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• pp.59-72
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• 2018

최근 급격히 증가하는 노인들과 정신적 휴식이 필요한 도시인들의 신체적 정신적 건강과 안녕감을 유지하기 위한 방법으로써 치유농업에 관한 다양한 연구가 이루어지고 있다. 본 연구는 치유농업 이용의도에 영향을 미치는 변인고찰을 위하여 노후준비도, 농촌치유 공급조건, 농촌치유 제공서비스를 독립변수로 정하여 연구하였다. 본 연구의 목적은 첫째, 치유농업 이용의도에 영향을 미치는 다양한 요인들을 실증적 분석을 통하여 파악하고자 한다. 둘째는 다양한 요인들 중에서 어느 변인이 이용의도에 더 많은 영향을 미쳤는지를 검증하고자 한다. 셋째, 이들 검증결과를 바탕으로 치유농업 활성화 정책을 제언하는 데 있다. 본 연구를 위해서 30세 이상 성인 남녀를 대상으로 설문조사를 실시하여 최종 356부를 분석대상으로 확정하였다. 연구가설의 검증은 선형회귀분석을 통하여 검증하였다. 분석결과는 다음과 같다. 첫째, 노후준비도의 신체적 준비와 정서적 준비 그리고 경제적 준비는 이용의도에 유의한 영향을 미치는 것으로 조사되었다. 둘째, 농촌치유 공급조건의 자연경관, 접근성, 안전성은 이용의도에 유의한 영향을 미치는 것으로 조사되었다. 그러나 경제성과 전문성은 기각되었다. 셋째, 농촌치유 제공서비스의 농작물 재배, 동물매개, 치유시설은 이용의도에 유의한 영향을 미치는 것으로 검증되었다. 이러한 연구를 통해, 치유농업의 이용의도를 높이기 위해서는 소비자의 노후준비가 잘되어 있어야 하며, 농촌치유 공급조건에서는 접근성과 안전성이, 농촌치유제공 서비스에서는 치유시설이 잘 갖춰져야 치유농업을 이용하려는 소비자의 이용의도를 높일 수 있다는 결론을 도출하였다.

• 생명과학회지
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• 제29권8호
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• pp.854-860
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• 2019

본 연구에서는 탈지된 갈색거저리 유충으로부터 효율적인 단백질 추출법 확립을 위한 최적 pH 조건을 조사하였다. 단백질 추출 pH 조건은 7부터 12까지 사용하였으며, 단백질 침전 pH는 2, 4, 그리고 6을 사용하였다. 이러한 조건에서 추출한 단백질의 함량, 갈변도 및 회수량을 분석한 결과 갈색거저리 유충 단백질의 최적 추출 조건은 단백질의 갈변도를 최소화 할 수 있으며 32.4%의 회수율을 나타내는 용출 pH 9.0와 침전 pH 4.0로 결정하였다. 다음으로 알칼리 추출법으로 추출한 갈색거저리 유충 단백질의 항염증 효능을 검토하였다. 갈색거저리 유충 단백질이 LPS에 의해 활성화 되는 대식세포의 염증반응 개선효과를 확인하기 위하여 염증매개 인자인 nitric oxide(NO)의 생성 및 iNOS와 COX-2 단백질의 발현량을 측정하였다. 그 결과 갈색거저리 유충 단백질은 세포독성 없이 NO의 생성을 억제 하였으며, MAPK 와 $NF-{\kappa}B$ 신호전달 억제를 통하여 iNOS와 COX-2 단백질의 발현량을 감소시켰다. 이러한 연구결과는 갈색거저리 유충 단백질이 항염증 치료제 및 단백질 보충제 개발을 위한 잠재적 가능성을 가지고 있는 것으로 사료된다.

• 한국농촌건축학회논문집
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• 제11권3호
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• pp.1-9
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• 2009

Through 'Local Theme Science Museum' aid program that is one of emphasis promotion strategic project by 2007 'Science.Technology.Cultural Project Plan', raises status of scientific technique by national core ability because all people expand base that understand and support scientific technique, and forms a social atmosphere which is scientific technique is important which scientific technique can be spread widely on national life and society whole, and proceeds target that expand local science museum facilities by a base of local science culture spreading and popularization. Accordingly, to plan balanced regional development and utilize to an advanced base of local science culture spreading because offer a science culture experience opportunity to local inhabitants, 'Local theme science museum' project is doing for that establish more 100 science museums for national.public.private institution in whole country until 2012 year. Support 39 projects to 2008 from 1999 thereby, in January, 2009, 13 'Local theme science museum' is opened and operated. This study examined arrangement preferentially about 'Science.Technology.Cultural Project Plan' that is background of 'Local theme science museum' project. Since surveying science museum which is on opening and operation at the present and analyzed actual conditions and facilities and investigated specification necessity and direction of 'Local theme science museum'. Hereafter, to seek plan for propriety of project by characteristic and difference of 'Local theme science museum' is purpose of this study. Now, need set and promotion of following specification direction for right activation of proceeding 'Local theme science museum' by each area. First, differentiation of theme and justifiability of building, Second, propriety of site position, Third, maximization of synergy effect by mixing concept of 'Specialized Economic Zone for Regional Developmental Policy' and Ecomuseum's 'Theme center'.

• 한국농촌건축학회논문집
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• 제15권1호
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• pp.1-11
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• 2013

The Gongju Sanseong Traditional Market, the subject of this study, was formed in the early 1900's, and along with the development of Gongju, it has been constantly changed and expanded up until now. The Sanseong Traditional Market located in Gongju, the Korea's representative historic city, exhibits merits in the aspects as a city based on the symbolic significance of the city where it is located and historical characteristics; however, it also presents some problems as well in consideration of user convenience and accessibility. Thereupon, this study conducted literature review on traditional markets, field research with a field trip, and a survey in order to analyze the spatial characteristics of the market environment and grope for the methods of activation. The conclusion suggested through this is as written below: First, the changes of types regarding spatial use according to the modernization project of traditional markets are essential, so about the spatial use according to the spatial changes, it is needed to distribute it balancedly considering the central space of the market. Second, with the understanding of the spatial types of traditional markets, it is needed to build a plan for alternation in the types of physical space based on that. Third, it is necessary to suggest the methods of integrated spatial use among standing markets and 5-day markets. In particular, through the approaches from the aspects of architectural and urban design and through reasonable spatial planning, a systematic approach considering the convenience of the users and merchants using it as their living space should be prioritized. And lastly, it is needed to reorganize the space according to the physical and non-physical methods in order to figure out the methods of activating traditional markets in agricultural areas and minor cities. With the complex approaches above, we can grope for the methods of activating traditional markets.

• The Plant Pathology Journal
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• 제32권6호
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• pp.552-562
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• 2016

Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

• 한국지역사회생활과학회지
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• 제16권1호
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• pp.37-47
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• 2005

Education has been emphasized as one of tools of realizing social equality, which has its base on the ideology of equal education opportunity. The Opportunity level of adult education is important in the meaning of social equality. but these days It is being inequality. so The objectives of this study were 1) measuring the opportunity level of adult education in Korea, 2) analysing the difference adult education level by gender, regions, 3) making out the gap of the its level by social educational agencies, 4) to suggest the way to improve the opportunity level of adults educations between adults by gender, regions. The followings are the results from the study: Adult education participation level were lower. Adult education participation level per adult education type showed higher participation at private institutes, lower at cultural center of community and school's human and material resources are not properly utilized in adult educational activities. By adult education participation level per gender and location, women have a higher participation level then men, and urban areas have a higher participation level then rural areas. In case of location, environment variables is most explanatory in adult education participation level. To strive for methods to activate school-oriented adult education activities and to develop political solution to ease the resources gaps for adult education investment among regions in order not to deepen environment gaps of adult education between urban and rural areas. For adult education activation, institution and bodies in charge of adult education should expand and discover hardware and develop and distribute software. Also, adult education specialists who operate and manage these hardware and sofeware efficiently should be recruited.

• 한국지역사회생활과학회지
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• 제22권4호
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• pp.689-704
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• 2011

Farm themed restaurants is an initiation by the Rural Development Administration (RDA) in order to perpetuate and develop Korean traditional food-culture. This food business model contributes to the increase of local food consumption and non-farm income for rural communities. Thus, the purpose of this study was to investigate the visitor's expectations, perception and satisfaction on the farm restaurant management type in order to assist activation of farm restaurants. Farm restaurants were categorized into two types; restaurant style and experience style. The majority of subjects(67.9%) were experiencing their first time to dine at farm restaurants. Regardless of farm restaurant style, the subjects perceived that farm restaurants use high quality food ingredients. The degree of subjects expectations prior to visiting a farm restaurant was estimated by 7 Likert scales. The results show that most subjects had high expectations for using good-quality food ingredients such as ‘fresh food (restaurant style 6.25, dietary education style 6.02)’ and ‘environment-friendly food (restaurant style 6.07, dietary education style 5.91)’. Overall satisfaction of the farm restaurants was also high. The satisfaction of food scored 4.1 and the satisfaction of experience program scored 3.8.

• 농촌계획
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• 제18권3호
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• pp.1-11
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• 2012

This study postulates a study model to examine the effect on the local economy revitalization of a selection of local resources, the local innovation capability strengthening, the establishment of promotion system and the creation of revenues for the participant groups of the local industry promotion project. To accomplish study purposes, 169 response samples from 85 project groups which drive the local industry promotion project were verified using SPSS 12.0 and AMOS 5.0. The results showed that firstly, the selection of local resources had a significant effect on the local innovation capability strengthening. Secondly, the local innovation capability strengthening had a significant effect on the establishment of promotion system. Thirdly, the establishment of promotion system had a significant effect on the creation of revenues. Fourthly, the selection of local resources didn't have an effect on the revitalization of local economy. Fifthly, both the local innovation capability strengthening and the establishment of promotion system didn't have an effect on the revitalization of local economy. Sixthly, the creation of revenues had a significant effect on the revitalization of local economy. According to the verifications of study model, the revitalization of local economy is achieved by inducing creation of revenues through the local innovation capability strengthening and the establishment of promotion system after the selection of local resources. From these results, this study presents suggestions, limits of study and directions in the future study.