• Korean Journal of Plant Resources
• /
• v.16 no.1
• /
• pp.55-60
• /
• 2003

The genetic variation of random amplified polymorphic DNA (RAPD) patterns of genomic DNAs was investigated in dioecious plant Rumex acetosa L., which carries different sex chromosome complements in female (2n=12A+XX) and male (2n=12A+XY$_1$Y$_2$). One hundred and twenty random primers consisted of 10-mer were used for PCR amplification. Polymorphic bands were found in 24 primers. Specific bands for female and male were 16 and 18, respectively. Especially, a band of 1,440 bp from the OPC-10 primer was male specific. These sex specific RAPD markers are used to understanding the sex determination mechanism in plants.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.48 no.3
• /
• pp.213-223
• /
• 2022

In this study, the anti-inflammatory and antioxidant effects of aerial parts of Rumex acetosa L. extract were confirmed to prevent various inflammatory diseases and skin aging caused by excessive oxidative stress. As a result of ABTS assay, it was confirmed that the radical scavenging ability increased in a concentration-dependent manner. ROS inhibitory ability was confirmed through DCF-DA assay, and concentration-dependent inhibition of ROS production was confirmed. The effect of inhibiting cell nuclear damage according to ROS was confirmed through DAPI staining. In addition, it was confirmed that the mRNA expression levels of iNOS and COX-2 were inhibited in a concentration-dependent manner through qPCR. As a result of confirming the protein levels of iNOS and COX-2 by western blotting, iNOS was significantly decreased at all concentrations, and COX-2 was significantly decreased at 800 ㎍/mL. The inhibitory effect on the production of NO generated by iNOS was confirmed by NO assay, and NO was decreased in a concentration-dependent manner. In addition, phosphorylation of ERK and JNK in the MAPKs signaling pathway were inhibited. Therefore, Rumex acetosa L. has the potential to be used as an anti-inflammatory and antioxidant cosmetic raw material by showing anti-inflammatory and antioxidant effects through the MAPKs pathway.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.2
• /
• pp.113-116
• /
• 2001

Chromosomal variation was investigated in the female and male somaclones regenerated from the leaf segment culture of Rumex acetosa L. Difference in phenotype depending on the sexuality was not observed. In female somaclones, 21 among 25 somaclones carried the same chromosome complements (2n＝14) with wildtype and others were tetraploids (2n=28), Considerable chromosomal variation was found in male somaclones. Only 4 among 20 somaclones carried normal chromosome number (2n=15) and 13 somaclones were aneuploids.

• Journal of Photoscience
• /
• v.12 no.2
• /
• pp.75-82
• /
• 2005

Rumex acetosa L. is a dioecious flowering plant with well developed sex chromosome system: 2n = 12 + XX in the female plants and 2n = 12 + XY1Y2 in the male plants. To isolate sex-linked DNA, we carried out chromosome micromanipulation, followed by DOP-PCR, AFLP of the PCR products, reverse Southern hybridization and sequence analysis. From 500 AFLP specific clones, 13 X-chromosome and 5 Y-chromosome specific clones were obtained. Except one clone RADAX-239 ($\underline{R}umex\;\underline{a}-\underline{D}OP-PCR-\underline{A}FLP-\underline{Y}-chromosome\;specific$), all clones appear to be R. acetosa plant-specific sequences and non-coding sequences. Southern blot analysis using these clones could not discriminate genomic DNAs either from male or female plants. Results of this study imply that both autosome-origin and degeneration of sex chromosomes are prevalent in plant systems.

• Korean Journal of Pharmacognosy
• /
• v.32 no.4 s.127
• /
• pp.338-343
• /
• 2001

The extract and fractions of Rumex acetosa (Polygonaceae), a Korean medicinal plant, were examined for their cytotoxicities against five cultured human tumor cell lines, i.e. A549 (non-small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system) and HCY15 (colon), using the SRB (sulforhodamine-B) method in vitro and antimutagenic activities by Ames test with Salmonella typhimurium TA98 and TA100 and SOS chromotest with E. coli PQ37. Among the tested samples, the methylene chloride fraction strongly inhibited the proliferation of each examined tumor cell line with $IC_{50}$ values ranged from 13.2 to $18.7\;{\mu}g/ml$ and showed potent antimutagenic activities with 96.1% and 96.7% at the concentration of 1 mg/plate against the mutagens, NPD and sodium azide, respectively. Its antigenotoxic activity was also very effective at the final concentration of $10\;{\mu}g/reaction$ tube against the mutagens, MNNG and NQO by SOS chromotest.

• Biomolecules & Therapeutics
• /
• v.20 no.4
• /
• pp.425-430
• /
• 2012

Rumex acetosa is a perennial herb that is widely distributed across eastern Asia. Although the hot water extract of R. acetosa has been used to treat gastritis or gastric ulcers as a folk medicine, no scientific report exists for the use of this plant to treat gastric ulcers. Hence, the present study was undertaken to assess the anti-ulcer activity of water and 70% ethanol extracts obtained from R. acetosa, using an HCl/ethanol-induced gastric ulcer model in mice. Anti-inflammatory and free radical-scavenging activities of these two extracts were also evaluated and compared. As a result, the administration of R. acetosa extracts significantly reduced the occurrence of gastric ulcers. However, significant differences in protective activity against gastric ulcers were observed between the two samples. In the case of the group pretreated with an ethanol extract dosage of 100 mg/kg, the protective effect (90.9%) was higher than that of water extract (41.2%). Under histological evaluation, pretreatment with R. acetosa extracts reversed negative effects, such as inflammation, edema, moderate hemorrhaging and loss of epithelial cells, presented by HCl/ethanol-treated stomachs. Meanwhile, R. acetosa extracts showed potent DPPH radical-scavenging activity and decreased NO production in a murine macrophage cell line, RAW 264.7, in a dose-dependent manner without affecting cellular viability. The greater anti-ulcer and NO production inhibitory activities exhibited by ethanol extracts compared to water extracts could be ascribed to the higher emodin levels, a major anthraquinone component of this plant.

• Proceedings of the Zoological Society Korea Conference
• /
• 1994.10a
• /
• pp.171.2-171
• /
• 1994

• International Journal of Industrial Entomology and Biomaterials
• /
• v.23 no.2
• /
• pp.215-221
• /
• 2011

To establish an indoor-rearing system for the Large Copper butterfly, $Lycaena$ $dispar$ and the Small Copper butterfly, $Lycaena$ $phlaeas$, the effect of temperature, photoperiod and host plants on larval development was investigated. The larvae of $Lycaena$ $dispar$ fed on $Rumex$ $crispus$, whereas the larvae of $Lycaena$ $phlaeas$ ate both $Rumex$ $crispus$ and $Rumex$ $acetosa$. The duration of the larval period of $Lycaena$ $dispar$ was 13.8 days on $R.$ $crispus$ and that, of $Lycaena$ $phlaeas$ was 15.9 days and 15.2 days on $R.$ $acetosa$ and $R.$ $crispus$ respectively. Laboratory experiments show that the Large Copper larvae are able to feed on other $Rumex$ species without harming their overall survival and can utilize these alternative host plants at least as efficiently as their natural host plant. This result suggests that plant chemistry is not responsible for their lack of utilization of the alternative host plants in the wild. Host plant choice by ovipositing females was measured with the two alternative hosts. $Lycaena$ $dispar$ preferred $R.$ $crispus$ to $R.$ $acetosa$, wheareas $Lycaena$ $phlaeas$ preferred $R.$ $acetosa$ to $R.$ $crispus$. Temperature has been proposed as an important determinant of developmental rate, lifespan and mortality in invertebrates. As temperature increased, length of the developmental period gradually decreased. The developmental period of the Large Copper larvae was 11.0 days and 28.5 days at $30^{\circ}C$ and $17.5^{\circ}C$, respectively.

• Proceedings of the PSK Conference
• /
• 2001.04a
• /
• pp.208.1-208.1
• /
• 2001

• Proceedings of the Zoological Society Korea Conference
• /
• 2003.08a
• /
• pp.188.3-188
• /
• 2003

No Abstract, See Full Text