Journal of Photoscience
- Volume 12 Issue 2
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- Pages.75-82
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- 2005
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- 1225-8555(pISSN)
Application of Chromosome Manipulation, DOP-PCR and AFLP Methods to Isolate Sex-Specific DNAs from Rumex acetosa L.
- Jin, Dong-Chung (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) ;
- Kim, Joong-Soon (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) ;
- Park, ji-Young (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) ;
- Bong, Jae-Wook (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) ;
- Hur, Yoon-Kang (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University)
- Published : 2005.08.31
Abstract
Rumex acetosa L. is a dioecious flowering plant with well developed sex chromosome system: 2n = 12 + XX in the female plants and 2n = 12 + XY1Y2 in the male plants. To isolate sex-linked DNA, we carried out chromosome micromanipulation, followed by DOP-PCR, AFLP of the PCR products, reverse Southern hybridization and sequence analysis. From 500 AFLP specific clones, 13 X-chromosome and 5 Y-chromosome specific clones were obtained. Except one clone RADAX-239 (
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