식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

수영 (Rumex acetosa L.) 암.수 체세포클론에서 염색체 변이

Chromosomal Variation in Female and Male Somaclones of Rumex acetosa L.

  • 김수영 (충남대학교 자연과학대학 생물학과) ;
  • 이미경 (충남대학교 자연과학대학 생물학과) ;
  • 김동순 (충남대학교 자연과학대학 생물학과) ;
  • 방재욱 (충남대학교 자연과학대학 생물학과)
  • 발행 : 2001.03.01
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초록

성염색체의 조성이 다른 수영 (Rumex acetosa L.) 암·수 개체의 잎 절편 배양을 통하여 얻은 체세포클론을 대상으로 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 암 개체의 경우 조사된 25개체 중 21개체 (84%)가 야생형과 같은 염색체 조성 (2n=14)을 보였으며, 야생형에서는 관찰되지 않는 4배체 식물이 4개체 (16%)가 관찰되었다. 그에 비하여 수 개체에서는 20개체 중 4개체 (20%)만이 정상의 염색체 조성을 보여 성에 따른 체세포클론 변이에서의 차이를 보였다. 수 개체에서는 2n=14, 28 등의 이수체가 관찰되었으며, 4배체 (2n=30)로 나타난 체세포클론에서도 성염색체의 뚜렷한 배가현상이 관찰되지 않았다.

Chromosomal variation was investigated in the female and male somaclones regenerated from the leaf segment culture of Rumex acetosa L. Difference in phenotype depending on the sexuality was not observed. In female somaclones, 21 among 25 somaclones carried the same chromosome complements (2n＝14) with wildtype and others were tetraploids (2n=28), Considerable chromosomal variation was found in male somaclones. Only 4 among 20 somaclones carried normal chromosome number (2n=15) and 13 somaclones were aneuploids.

키워드

참고문헌

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