• Archives of Pharmacal Research
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• 제16권2호
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• pp.109-113
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• 1993

The dried unnipe fruits of Rubus sp. (Rubi Fructus, Bogabunja) have yielded $\beta$-sitosterol glucoside and four urs 12 en-28-oic acid derivatives, three of which were as their glucosiders. They were identified as 23-hydroxytomentic acid, rosamultin, niga-ichigosides $F_1\;and\;F_2$ on the basis of spectral data.

• 한국자원식물학회지
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• 제19권5호
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• pp.563-572
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• 2006

The plant Rubus species (Rosaceae) mainly contains $19{\alpha}-hydroxyurane-type$ triterpenoids $(19{\alpha}-HUT)$ as bioactive components. Available functional food includes blackberry (the fruit of thornless Rubus sp.), red raspberry (R. idaeus) and black raspberry (R. occidentalis). However, the fruit of R. coreanus, which is used in Korea as a functional food, substitutes black raspberry. Rubi Fructus, which has been traditionally used as an oriental medicinal drug, designates only unripe fruit of R. coreanus but not its ripe fruit which indicates that it needs high content of $19{\alpha}-HUT$ as a crude drug. Throughout our experiment, we found that ripe fruits contain very little amount of $19{\alpha}-HUT$ when compared to unripe fruits. In addition, various and rich $19{\alpha}-HUT$ has been reported from Rubus species. The most common structure of $19{\alpha}-HUT$ of Rubus species, euscaphic acid or tormentic acid with $3{\alpha}-OH$ or $3{\beta}-OH$, respectively, can be glycosylated or oxidized to produce a number of $19{\alpha}-HUTs $as euscaphic acid and tormentic acid derivatives and even esterified to form dimeric triterpenoids. In this review, the bioactivity and biosynthetic pathway and chemical characterization of $19{\alpha}-HUTs$ found in Rubus species are discussed.

• 한국토양비료학회지
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• 제41권5호
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• pp.310-317
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• 2008

포화추출액중 전기전도도는 $1.0\sim28.4dS\;m^{-1}$의 범위로 평균 $4.8dS\;m^{-1}$를 나타내어 매우 높은 농도로 염류가 집적되고 있었다. 전기전도도 $4dS\;m^{-1}$ (포화추출액 조건으로 환산)를 초과한 경우가 전체의 55%를 차지하고 있었다. 복분자 비가림하우스 재배토양 중 전기전도도와 각각의 무기이온과의 단순상관관계를 조사한 결과, 전기전도도와 염소 ($r=0.85^{**}$), 질산 ($r=0.94^{**}$), 인산 ($r=0.88^{**}$), 황산 ($r=0.84^{**}$) 이온과 같은 음이온과 고도의 유의성 있는 정(+)의 상관관계를 나타내었고, 마그네슘 ($r=0.41^*$), 칼슘 ($r=0.38^*$), 칼리 ($r=0.47^*$) 이온과 같은 양이온과는 유의성 있는 정(+)의 상관관계를 나타내었다. 비가림 하우스형 복분자 재배토양 중에 함유되어 있는 염류집적 유발물질을 제거하는데 있어 작업의 편이성, 농업용수의 절감 및 처리시간 등을 고려하였을 때 용탈법보다 수세법이 더 효과적인 것으로 나타났다.

• 식물병연구
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• 제22권2호
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• pp.116-121
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• 2016

In 2015, stem blight of Rubus crataegifolius was observed in Pohang, Korea. The symptoms began as dark red spots in the stem, which led to stem blight, then leaf blight, and eventually resulted in death. A fungal isolate was obtained from a symptomatic stem and incubated on a potato dextrose agar plate. The isolated fungus produced white, cloudy mycelia turned black in 3 days. Based on the morphological characteristics, the causal fungus was assumed to be Botryosphaeria sp. A pathogenicity test was conducted according to Koch's postulates. To identify the causal agent, the combined sequence of the internal transcribed spacer, ${\beta}$-tubulin, and translation elongation factor $1{\alpha}$ genes were used for phylogenetic analysis. Approximately 1,200 bp of the combined sequence clearly suggested that the isolated pathogen was Botryosphaeria parva. This is the first report on stem blight in R. crataegifolius caused by B. parva in Korea.

• 식물병연구
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• 제27권4호
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• pp.187-191
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• 2021

Wilt symptoms were frequently observed in Korean blackberry (Rubus coreanus) plants grown in farmers' fields located in Gochang and Jeongeup, Jeonbuk Province, Korea during disease surveys in July 2020. The disease occurred in 10 of the 13 fields surveyed in the two locations. The incidence of diseased plants in the fields was 5-80%. Seven isolates of Fusarium sp. were obtained from the diseased plants and examined for their morphological and molecular characteristics. All the isolates belonged to Fusarium oxysporum species complex based on the morphological characteristics but were identified as F. cugenangense based on the molecular characteristics. Two isolates of F. cugenangense were tested for pathogenicity on Korean blackberry plants by artificial inoculation. Pathogenicity of the two isolates on the plants was confirmed with the inoculation tests, which showed wilt symptoms similar to those observed in the diseased plants in the fields investigated. This is the first report of F. cugenangense causing Fusarium wilt in Korean blackberry.

• 한국식품과학회지
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• 제43권2호
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• pp.149-155
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• 2011

4종류의 다공성 합성흡착제(Sepabeads 207, 700, 850, Diaion HP 20)를 사용하여 Rubus coreanus 과실의 물 추출물로부터 과당 및 비polyphenol성분을 효과적으로 제거하여 DPPH라디칼 소거능 및 화학발광법을 이용한 superoxide 라디칼 소거능이 증가된 농축물을 효율적으로 얻을 수 있었다. 또한, 실시간 HPLC $ABTS^{{\cdot}+}$ 활성분석 시스템을 활용하여 6개의 항산화 능력을 갖는 활성피크를 확인할 수 있었으며 이들 대상으로 LC-MS/MS분석을 실시하여 5개의 anthocyanin인 cyanidin-3-sambubioside, cyanidin-3-glucoside, cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside, pelargonidin-3-rutinoside를 mass pattern 및 분자량 확인 과정을 통해 잠정적 화학구조 동정을 하였다. 수율과 항산화 효능을 고려해 볼 때, 4종의 합성흡착제 중에서 Diaion HP 20이 가장 우수한 결과를 나타내었다. 이처럼 적절한 합성흡착제의 선택으로 표적물질에 대한 polyphenol성 물질의 농축뿐만 아니라 생리학적 활성증대효과를 가져올 수 있어 적절한 합성흡착제의 선택은 식품 및 의약품의 정제 및 분리에 유용하게 활용될 수 있음이 확인되었다.

• BMB Reports
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• 제41권7호
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• pp.529-536
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme in the phenylpropanoid pathway, which is responsible for synthesizing a variety of secondary metabolites that participate in development and adaptation. In this study, we isolated a full-length cDNA of the C4H gene from the Korean black raspberry (Rubus sp.) and found that this gene existed as a single gene. By comparing the deduced amino acid sequence of Rubus sp. C4H with other sequences reported previously we determined that this sequence was highly conserved among widely divergent plant species. In addition, quantitative real time PCR studies indicated that the C4H gene had a differential expression pattern during fruit development, where gene expression was first detected in green fruit and was then remarkably reduced in yellow fruit, followed by an increase in red and black fruit. To investigate the two peaks in expression observed during fruit development and ripening, we measured the flavonoid content. The content of the major flavanol of Korean black raspberry fruits was determined to be highest at the beginning of fruit development, followed by a gradually decrease according to the developmental stages. In contrast, the content of anthocyanins during the progress of ripening was dramatically increased. Our results suggest that the C4H gene in Korean black raspberry plays a role during color development at the late stages of fruit ripening, whereas the expression of C4H gene during the early stages may be related to the accumulation of flavanols.

• 한국환경복원기술학회지
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• 제7권1호
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• pp.59-67
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• 2004

This study was carried out to select proper species for early stage replantation in granite forest roads cut-slope. From one to fourteen year elapsed forest roads in eight regional areas of Gyeongsang-do and Jeolla-do, sample plots were selected, and their vegetations and environmental factors were investigated. Soil organic matter, total nitrogen, soil moisture content, and bulk density in the granite weathering soils were below 0.4%, 0.03%, 12.4%, and above 1.2g/$cm^3$, respectively. The result indicated that the soil was not good for plant growth. The environmental factors affecting distribution of species in the granite forest roads were the elapsed year, elevation, and cut-slope aspect. Invaded species with high frequency in the forest road cut-slope ordered Pinus densiflora, Lespedeza bicolor, Rubus crataegifolius, Alnus hirsuta, Youngia denticulata, Patrinia villosa, Artemisia princeps var. orientalis, Zanthoxylum schinifolium, and Dactylis glomerata. Herbs seeded in earth stage such as Dactylis glomerata, Eragrostis curvula, Festuca arundinacea, Lespedeza sp., and Amorpha fruticosa had changed into perennial herbs and shrubs such as Youngia denticulata, Patrinia villosa, Aster scaber, Patrinia scabiosaefolia, Melica onoei, Astilbe chinensis var. davidii, Chrysanthemum zawadskii var. latilobum, Pueraria thunbergiana, Stephanandra incisa, and Clematis apiifolia, and had changed into plants such as Artemisia princeps var. orientalis, Artemisia keiskeana, Miscanthus sinensis, Pinus densiflora, Rhododendron mucronulatum, Rubus crataegifolius, and Zanthoxylum schinifolium according to elapsed year of forest roads. Leguma such as Lespedeza sp., Pueraria thunbergiana, Amorpha fruticosa, etc., herbs such as Artemisia keiskeana, Artemisia princeps var. orientalis, Chrysanthemum zawadskii var. latilobum, Miscanthus sinensis, etc., and woody plants such as Pinus densiflora, Rubus crataegifolius, Rhododendron mucronulatum, etc., with foreign herbs were considered as proper species for replantation in granite forest road cut-slope.

• 한국환경복원기술학회지
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• 제8권6호
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• 한국환경복원기술학회지
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• 제7권6호
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• pp.84-93
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• 2004

This study was carried out to select proper species for early stage replantation in granite forest roads cut-slope. From two to sixteen year elapsed forest roads in four regional areas of Gangwon-do, sample plots were selected, and their vegetations and environmental factors were investigated. Soil organic matter, total nitrogen, soil moisture content, and bulk density in the decomposed granite soils were below 1.32%, 0.08%, 10.0%, and above 1.24g/$cm^3$, respectively. The result indicated that the soil was not good for plant growth. The environmental factors affecting distribution of species in the granite forest roads were the elapsed year, cut-slope, elevation, and cut-slope aspect. Species with high frequency in the granite forest road cut-slope ordered Lespedeza bicolor, Rubus crataegifolius, Pinus densiflora, Lysimachia clethroides, Amorpha fruticosa, Dactylis glomerata, Lespedeza cyrtobotrya, Festuca arundinacea, Spodiopogon sibiricus, Artemisia stolonifera, and Weigela subsessilis. Herbs seeded in earth stage such as Dactylis glomerata, Lolium perenne, and Festuca arundinacea had changed into perennial herbs such as Carex lanceolata, Poa sphondylodes, Patrinia villosa, Artemisia princeps var. orientalis, Spodiopogon sibiricus, Melica onoei, Artemisia keiskeana, Artemisia stolonifera, Astilbe chinensis var. davidii, Spodiopogon sibiricus, and those had changed into shrubs such as Rubus crataegifolius and Weigela subsessilis, and those had changed into trees such as Salix hulteni, Salix koreensis, and Fraxinus rhyncholhylla according to elapsed year of forest roads. Legumes such as Lespedeza sp., Pueraria thunbergiana, Amorpha fruticosa, etc., herbs such as Artemisia keiskeana, Artemisia princeps var. orientalis, Artemisia stolonifera, Astilbe chinensis var. davidii, Spodiopogon sibiricus, Miscanthus sinensis, etc., and woody plants such as Pinus densiflora, Rubus crataegifolius, Weigela subsessilis, Zanthoxylum schinifolium, Rhododendron mucronulatum, etc., with foreign herbs were considered as proper species for replantation in granite forest road cut-slope.