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http://dx.doi.org/10.5423/RPD.2016.22.2.116

First Report of Botryosphaeria parva Causing Stem Blight on Rubus crataegifolius in Korea  

Park, Sangkyu (School of Applied Biosciences, Kyungpook National University)
Kim, Seung-Han (Gyeongsangbuk-do Agricultural Research & Extension Services)
Back, Chang-Gi (School of Applied Biosciences, Kyungpook National University)
Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University)
Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University)
Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.22, no.2, 2016 , pp. 116-121 More about this Journal
Abstract
In 2015, stem blight of Rubus crataegifolius was observed in Pohang, Korea. The symptoms began as dark red spots in the stem, which led to stem blight, then leaf blight, and eventually resulted in death. A fungal isolate was obtained from a symptomatic stem and incubated on a potato dextrose agar plate. The isolated fungus produced white, cloudy mycelia turned black in 3 days. Based on the morphological characteristics, the causal fungus was assumed to be Botryosphaeria sp. A pathogenicity test was conducted according to Koch's postulates. To identify the causal agent, the combined sequence of the internal transcribed spacer, ${\beta}$-tubulin, and translation elongation factor $1{\alpha}$ genes were used for phylogenetic analysis. Approximately 1,200 bp of the combined sequence clearly suggested that the isolated pathogen was Botryosphaeria parva. This is the first report on stem blight in R. crataegifolius caused by B. parva in Korea.
Keywords
Botryosphaeria parva; Combined sequence; Rubus crataegifolius; Stem blight;
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