• Journal of ILASS-Korea
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• v.7 no.4
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• pp.32-41
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• 2002

The breakup characteristics of liquid sheets formed by like-doublet injector were investigated in the cold-flow and atmospheric ambient pressure condition. The sheet breakup wavelength, which induces the sheet to be broken into ligaments, as well as the sheet breakup length, which is important for the flame location, was measured using a stroboscopic light. The liquid ligaments are formed intermittently after the breakup of sheet, and the wavelength of ligaments has been believed to have a relation to the combustion instability of liquid rocket engine. Therefore, the wavelength of ligaments and the breakup length of ligaments into fine drops were also measured. Since these spray characteristics are affected by the flow characteristics of two liquid jets before they impinge on each other, we focused on the effects of orifice internal flow such as the cavitation phenomenon that occurs inside the sharp-edged orifice. From the experimental results, we found that the liquid jet turbulence delays the sheet breakup and makes shorter wavelengths for both sheets and ligaments. Since the turbulent strength of sharp-edged orifice is stronger than that of round-edged orifice, the shape of orifice entrance results in large differences in the spray characteristics. Using these results, we proposed empirical models on the spray characteristics of the like-doublet injector, and these models are believed to provide some useful and actual data for designing liquid rocket combustors.

• Journal of the Korean Applied Science and Technology
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• v.27 no.4
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• pp.508-514
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• 2010

Regenerated cellulose fibers were prepared from three pulps containing different degree of polymerization(DP) and $\alpha$-cellulose contents by dry-jet wet spinning technique with cellulose dope in N-methylmorpholin N-oxide (NMMO). The effect of antioxidant, n-propyl gallate (PG) on the properties of different regenerated celluloses was studied using X-ray diffraction, copper number calculation, and viscometry. The degradaqtion of regenerated cellulose from pulp containing higher DP and lower $\alpha$-cellulose content was occurred more seriously. The tensile strength and initial modulus of regenerated cellulose fiber obtained from NMMO dope with PG were higher than those of fiber obtained from NMMO dope without PG. All fibers showed the round shape cross section and typical cellulose II crystalline structure.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.5
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• pp.962-978
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• 1989

A new model of the combustion rates of soot particle in turbulent flames has been suggested. This model applies the combustion rate of soot particles in laminar flames and uses local time-averaged quantities in order to consider the effect of the chemical reaction on the soot combustion in turbulent flames. The proposed rate equation has been tested for two propane-air turbulent round-jet diffusion flames and gives better predictions for the soot concentration field of two flames than the model previously used, especially in low temperature regions. A modified Monte carlo Method for analyzing radiative heat transfer of a flame also has been suggested and tested, which reveals good results.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1546-1551
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• 2004

The laser Doppler technique is well-established as a velocity measurement technique of high precision for flow velocity. Recently, the laser Doppler technique has also been used to measure acceleration of fluid particles. Acceleration is interesting from a fluid mechanics point of view, since the Navier Stokes equations, specifically the left-hand-side, are formulated in terms of fluid acceleration. Further, there are several avenues to estimating the dissipation rate using the acceleration. However such measurements place additional demands on the design of the optical system; in particular fringe non-uniformity must be held below about 0.0001 to avoid systematic errors. Relations expressing fringe divergence as a function of the optical parameters of the system have been given in the literature; however, direct use of these formulae to minimize fringe divergence lead either to very large measurement volumes or to extremely high intersection angles. This dilemma can be resolved by using an off-axis receiving arrangement, in which the measurement volume is truncated by a pinhole in front of the detection plane. In the present study an optical design study is performed for optimizing laser Doppler systems for fluid acceleration measurements. This is followed by laboratory validation using a round free jet and a stagnation flow, two flows in which either fluid acceleration has been previously measured or in which the acceleration is known analytically. A 90 degree off-axis receiving angle is used with a pinhole or a slit.

• Journal of the Korean Society of Propulsion Engineers
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• v.7 no.1
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• pp.28-39
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• 2003

Effects of the orifice internal flow such as cavitation and hydraulic flip on transverse injection into subsonic crossflows have been studied. The liquid column breakup length and the liquid column trajectory were measured by changing the orifice diameter (d), the orifice length/orifice diameter (L/d), the injection pressure and the shapes (sharp and round) of orifice entrance, and were compared with previous results. It is found that cavitation bubbles, which occur inside the sharp-edged orifice, make the liquid jet very turbulent and especially in the orifices with L/d ＝ 5 hydraulic flip appear as cavitation bubbles are emitted from the orifice. The breakup length is shorter as cavitation bubbles grows and hydraulic flip appears. However, the liquid column trajectories normalized by the effective diameter and the effective momentum ratio have a similar tendency irrespective of cavitation and hydraulic flip.

• Journal of the Korea Institute of Military Science and Technology
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• v.21 no.2
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• pp.195-203
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• 2018

Among the techniques of reducing the drag to increase the speed of underwater moving bodies, we studied on the drag reduction method by gas injection. Researches on gas injection method have been paid much attention to reduce the drag of vessels or pipe inner walls. In this study, we used a sintered metal mesh that can uniformly distribute fine bubbles by gas injection method, and applied it to a cylindrical underwater moving body. Using the KRISO medium-sized cavitation tunnel, we measured both the bubble size on the surface of the sintered mesh and the bubble distribution in the boundary layer. Then, drag reduction tests were performed on the cylinder type underwater moving models with cylindrical or round type tail shape. Experiments were carried out based on the presence or absence of tail jet injection. In the experiments, we changed the gas injection amount using the sintered mesh gas injector, and changed flow rate accordingly. As a result of the test, we observed increased bubbles around the body and confirmed the drag reduction as air injection flow rate increased.

• Journal of Embryo Transfer
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• v.29 no.4
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• pp.351-359
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• 2014

Phasianus colchicus is not only a beautiful bird but also a great value in science and under the threat of endanger. Hence, the aim of this study was to isolate the pheasant male germ cells (mGCs) and then induce them into elongated sperm-like cells in vitro. The mGCs were purified and enriched by a two-step plating method based on the different adherence velocities of mGCs and somatic cells. The percentage of the c-kit positive cells and c-kit negative cells examined by flow cytometry analysis (FCA) was 92.87% and 2.57%, respectively. Subsequently, the mGCs were induced for 48h in DMEM/F12 medium supplemented factors such as retinol acid, testosterone and bovine FSH, followed by 5 weeks in culture. We found that some elongated sperm-like cells appeared initially in vitro under inducement of stimulated factors. The elongated sperm-like cells showed in the expression of changed morphology and post-transcriptional marker such as spermatid associated (SPERT), spermatid perinuclear RNA binding protein (STRBP), round spermatid basic protein 1 (RSBN1) and SPER1L. Moreover, in DNA content identified assay, induced cells showed that the 1C DNA population markedly increased in differentiated group but it was not change in undifferentiated group. Successful in vitro differentiation of pheasant testicular germline cells into spermatids appears to offer extremely attractive potential for the conservation of endangered birds and treatment of male infertility.

• Journal of Embryo Transfer
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• v.28 no.4
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• pp.337-342
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• 2013

Salmonellosis is one of the life-threating diseases of goat in Bangladesh. Therefore, the present study was designed to study the prevalence of Salmonellosis, and isolation and characterizations of the Salmonella spp. from apparently healthy and diarrheic goat. A total of 47 faces samples were collected from selected place and cultured onto different prescribed medium to isolate it. In this study, 12.76% (6/47) samples were found to be positive for Salmonella spp. During culture on SS agar medium, all of the Salmonella isolates produced round, smooth, opaque, translucent and black color colonies on SS agar media. All of the isolated Salmonella spp. fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. However, these isolates had showed Indole and Voges-Proskauer test negative, Methyl-Red test positive. All of these isolates were subjected to rapid plate agglutination test with polyvalent "O" (Poly 'O') and polyvalent "H" (poly 'H') antisera where positive agglutination were observed. They were highly sensitive to ciprofloxacin, spiramycin and gentamycin; moderately sensitive to oxytetracyline, streptomycin and amoxicillin; less sensitive to sulphamethoxazole and resistant to penicillin-G. Based on the present findings, it may be concluded that the investigated Salmonella spp. from goats might be S. typhimurium, S. enteritidis, S. brandenburg, S. salford, S. newbrunswick, S. newport or S. dublin. Further study will be needed, therefore it requires further characterization using other serological and molecular techniques.

• Journal of Animal Environmental Science
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• v.18 no.3
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• pp.207-220
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• 2012

There are around 7,500 manure tanks to treat the manures from pigs in Korea. In the tank, there are too much sediments deposited on the base and wall, which causes low efficiency of stock capacity and manure fermentation. In order to minimize sediments and to ferment manure effectively, we developed a 2-fluid jet mixer for mixing sediments in liquid livestock manure tank. For developing the prototype, we tested a factorial experimental system with various nozzles, and simulated CFD models with two kinds of nozzle arrangement. From the results of factorial experiment and CFD simulation, we concluded the dia. ratio of primary : secondary nozzle should be 1:2 and the nozzles should be arranged at the same distances toward to the circumferential direction. With this results, we manufactured a 2-fluid jet mixer which is consists of four 2-phase nozzles, centrifugal slurry pump and root's type air blower. And, we carried out the performance test of the prototype in the round shaped liquid manure tank in the farm. The performance test results showed that the uniformity of TS (Total Solid) and VS (Volatile Solid) was raised from 21.3 g/L, 13.3 g/L In steady state to TS and VS to 23.0 g/L, 14.1 g/L in the mixing operation. Therefore, we could conclude that the prototype of 2-fluid mixer could make the solid material which could be sediments in the tank not to be deposited in the tank and to be contacted to air bubbles which could enhance the efficiency of the fermentation of livestock manure.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.249-255
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• 2015

Diabetes mellitus, the most common metabolic disorder, is divided into two types: type 1 and type 2. The essential treatment of type 1 diabetes, caused by immune-mediated destruction of ${\beta}-cells$, is transplantation of the pancreas; however, this treatment is limited by issues such as the lack of donors for islet transplantation and immune rejection. As an alternative approach, stem cell therapy has been used as a new tool. The present study revealed that bone marrowderived mesenchymal stromal cells (BM-MSCs) could be transdifferentiated into pancreatic cells by the insertion of a key gene for embryonic development of the pancreas, the pancreatic and duodenal homeobox factor 1 (PDX1). To avoid immune rejection associated with xenotransplantation and to develop a new cell-based treatment, BM-MSCs from ${\alpha}$-1,3-galactosyltransferase knockout (GalT KO) pigs were used as the source of the cells. Transfection of the EGFP-hPDX1 gene into GalT KO pig-derived BM-MSCs was performed by electroporation. Cells were evaluated for hPDX1 expression by immunofluorescence and RT-PCR. Transdifferentiation into pancreatic cells was confirmed by morphological transformation, immunofluorescence, and endogenous pPDX1 gene expression. At 3~4 weeks after transduction, cell morphology changed from spindle-like shape to round shape, similar to that observed in cuboidal epithelium expressing EGFP. Results of RT-PCR confirmed the expression of both exogenous hPDX1 and endogenous pPDX1. Therefore, GalT KO pig-derived BM-MSCs transdifferentiated into pancreatic cells by transfection of hPDX1. The present results are indicative of the therapeutic potential of PDX1-expressing GalT KO pig-derived BM-MSCs in ${\beta}-cell$ replacement. This potential needs to be explored further by using in vivo studies to confirm these findings.