• Journal of Pharmacopuncture
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• v.12 no.4
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• pp.51-62
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• 2009

Objective & Methods : The purpose of this study was to observe the effects of herbal-acupuncture with Rosae Laevigatae Fructus extact(RLF-HA) at KI10(Umgok) on osteoporosis in ovariectomized( OVX) ddy mice. We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : 1. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) and saline injection at KI10(Umgok) significantly inhibited the reduction of tibial calcium level in ovariectomized mice. 2. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice. 3. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the reduction of tibial trabecular bone thickness(TBT) in ovariectomized mice. 4. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the overgrowth of tibial growth plate length(GPL) in ovariectomized mice. Conclusion : These results suggested that herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) has a therapeutic effect on osteoporosis, and to be put to practical use in the future osteoporosis clinic.

• Herbal Formula Science
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• v.12 no.1
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• pp.247-254
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• 2004

Objective: Effects of keumengja extracts on the blood picture in rat were determined. Results: Values of RBC, PCV and Hb showed a high in keumenja groups, however the values of PCV and Hb showed no significantly different in keumenja groups. Counts of WBC showed no significantly different in all treatment groups. In the composition of WBC neutrophils showed a tendance to decrease in keumengja groups, however these values showed no significantly different. Lymphocytes showed a high portion in keumengja groups and this portion showed a tendance to increase to increased concentration of keumengja. The portion of monocytes and basophils showed no significantly different in all treatment groups. Eosinophils showed a tendance to decrease in keumengja groups.

• The Journal of Internal Korean Medicine
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• v.19 no.1
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• pp.452-465
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• 1998

Rosae laevigatae Fructus extract (RLF) was tested for the effects on the urethral nitric oxide synthase (NOS) activity and Antioxidation in streptozotocin (STZ) induced diabetic rats. RLF was treated firstly into samples, and then STZ induced diabetic rats were set with them. In vitro, the urethral NOS activity was not noted but the type O activity and type conversion ratio of xanthine oxidase and the level of urethral lipid peroxide were decreased in the level of Dose of extract prepared from RLF. In vivo, after the extract was administered to the animal model for fifteen days, the urethral NOS activity increased in STZ induced diabetic rats to the level of normal rats. The content of urethral nitrite and glutathione followed by RLF pre-medicating administration, increased as highly as normal group in compare with the group treated with STZ. The type O activity and type conversion ratio of xanthine oxidase and the level of urethral lipid peroxide followed by RLF pre-medicating administration, decreased as lowly as normal group in compare with the group treated with STZ. In conclusion, the extract of RLF will be able to restore erectile dysfunction of STZ induced diabetic rats.

• Journal of Haehwa Medicine
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• v.10 no.1
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• pp.167-174
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• 2001

According to the literatual study on the external treatment of premature ejaculation, the results were as follows. 1. Linition is inunction glans penis after infiltrate ASARI HERBA CUM RADICE(細辛), CARYOPHYLLI FLOS(丁香), CHEBULAE FRUCTUS(訶子), PAPAVERIS FRUCTUS(罌粟殼), FOSSILIA OSSIS MASTODI(龍骨), OSTREAE CONCHA(牡蠣 in oenostagma. 2. Retrojection or hypoatmism method is boiling CHINENSIS GALLA(五倍子), CNIDII FRUCTUS(蛇牀子) and hypoatmism, down temperature infiltrate glans penis. 3. Mesompharion apposition method is calorization VESPAE NIDUS(露蜂房), ANGELICAE DAHARICAE RADIX(白芷), and utilization vineger apposite mesompharion. 4. Medicament belt method is belt on lumbus and bythus by ROSAE LAEVIGATAE FRUCTUS(金櫻子), EURYALES SEMEN, OSTREAE CONCHA(牡蠣), TRIBULI FRUCTUS, AIPINIAE OXYPHYLLAE FRUCTUS(益智仁), NELUMBINIS SEMEN(蓮子肉) Powders. 5. Enema is injection in the rectum ANGELICAE GIGANTIS RADIX(當歸) effusion. 6. Much utilization agent are ASARI HERBA CUM RADICE(細辛), CHINENSIS GALLA(五倍子), OSTREAE CONCHA(牡蠣), CNIDII FRUCTUS(蛇床子), GRANATI PERICARPIUM(石榴皮), CARYOPHYLLI FLOS(丁香), CHEBULAE FRUCTUS(訶子), FOSSILIA OSSIS MASTODI(龍骨), VESPAE NIDUS(露蜂房), PAPAVERIS FRUCTUS(罌粟殼) etc. They are occupied in adstringentia, juventia, divergence agent, liver stabilizer agent.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.2
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• pp.127-140
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• 2006

Purpose : This study was conducted to investigate the antimicrobial effects of Astringent medicinals against vaginal microbe. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans and Gardnerella vaginalis were used for vaginitis-induced microbe. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for normal vaginal florae. And Astringent medicinals (Rubi Fructus, Tritici Immatri Semen, Corni Fructus, Nelumbinis Semen, Mume Fructus, Schizandrae Fructus, Ailanthi Radicis Cortex, Galla Rhois, Myristicae Semen, Terminariae Fructus, Rosae laevigatae Fructus, Ephedrae Radix) were used in this study. Antimicrobial activities were tested by optical density and colony test in vitro. And then wee valuated the antimicrobial effects in comparison with optical density and colony test. Results : The optical density and colony test showed that Terminariae Fructus and Galla Rhois among Astringent medicinals had the antimicrobial effects. Teminariae Fructus had the antimicrobial susceptibility and selective toxicity against Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Galla Rhois had the antimicrobial susceptibility and selective toxicity against Staphylococus aureus, Methicillin-resistant Staphylococus aureus, Candida albicans and Gardnerella vaginalis of vaginitis-induced microbe. Conclusion : According to these results, we can suggest that Terminariae Fructus and Galla Rhois among Astringent medicinals be available to the antimicrobial agent of vaginitis-induced microbe in vitro.

• Microbiology and Biotechnology Letters
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• v.44 no.1
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• pp.89-97
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• 2016

Obesity is caused by excess accumulation of body fat and contributes to various pathological disorders such as diabetes, hypertension, cardiovascular disease, and cancer. In this study, we investigated the effect of a 30% ethanol extract of Fructus Rosae laevigata (RLE) on adipogenesis in 3T3-L1 adipocytes, measured by triglyceride accumulation and expression of adipogenesis-related transcription factors during differentiation of pre-adipocytes into adipocytes. RLE decreased the intracellular triglyceride contents (assessed by Oil Red-O staining) in a dose-dependent manner. It also downregulated the expression of adipogenic transcription factors and inhibited cell proliferation during the mitotic clonal expansion phase of adipocyte differentiation by inducing G1 phase arrest. We investigated the alterations in the levels of G1 phase arrest-related proteins. The expression of p21 protein significantly increased, while the levels of Cyclin E, Cdk2, and phospho-Rb decreased in a dose-dependent manner in 3T3-L1 cells treated with RLE. These results suggest that RLE inhibits the differentiation of 3T3-L1 adipocytes by suppressing the expression of adipogenic transcription factors and inducing G1 phase arrest in the early stages of adipocyte differentiation.