• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.364-370
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• 2014

The nutrient and irrigation management of scion and rootstock can alleviate stress on grafted transplants after grafting and promote the growth. This study investigated the effects of nutrient and irrigation management of scion and rootstock on the graft-take and growth of grafted pepper transplants. Before grafting, the scions were subjected to different water potential regimes in media by controlling the irrigation frequency and time. The scions were subirrigated 0, 1(two days before grafting), 1(one days before grafting) or 2 times for five days before grafting. The irrigation frequency and time influenced the water potential of media and the growth of scion and grafted transplants. At 13 days after grafting, fresh and dry weight of transplants which were irrigated once at two days before grafting were greater by 29 and 34% than those without irrigation during five days before grafting. This suggests that mild water stress on scion prior to grafting by controlling water management alleviate water stress on grafted transplants after grafting and improve the growth. Before grafting, the rootstocks were subjected to different nutrient regimes by controlling nutrient solution application. The rootstocks were supplied with nutrient solution 0, 1, 2, or 4 times. The nutrient application frequency and time influenced the electrical conductivity (EC) and pH of media. Accordingly, the growth and mineral contents of rootstock and grafted transplants were also affected. At 13 days after grafting, fresh and dry weight of transplants with four times of nutrient application increased by 30 and 20%, respectively, than those without nutrient solution supply during seven days before grafting. Therefore, it is recommended that nutrient solution be supplied more than four times during seven days before grafting for the production of high quality transplants.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.228-235
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• 2019

Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

• Natural Product Sciences
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• v.13 no.3
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• pp.241-246
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• 2007

Andrographis stenophylla C. B. Clarke., (Acanthaceae) is an erect glabrous undershrub with very narrow leaves and stems from a stout rootstock, the corolla pale with dark red stripes. The plant grows in hills of about 1200 meters height in South India. No scientific work reports are available with regard to this plant. The present study, thus deals with the detailed pharmacognostical evaluation of the plant A. stenophylla using light and confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of A. stenophylla and can possibly help to differentiate the drug from its other species.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.70.2-70
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• 2003

To evaluate the resistance to crown gall in grape rootstocks, cuttings from twenty seven grape rootstocks were inoculated with Agrobacterium vitis Cheonan 493 and size of galls from grapevines was measured in a greenhouse. Tumors were formed in all varieties of grape rootstocks tested in this study and no grape rootstock variety was immune to crown gall. Tumors were found on the stems of all plants tested in '196-17'and '41B' Based on measuring size and weight of galls formedon the stem of grape rootstocks, '779P' was extremely susceptible to crown gall. Some varieties such as 'Gloire', '140R', '101-l4M', '3309C', and '333EM' found to be resistant, while '99R', '1447P', 'Rupestris du lot', '110R', 'Freedom', and '41B'were susceptible and '1103P', '5C', '420A', 'Golia', and '5BB' were moderately susceptible to crown gall.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.632-638
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• 2000

This paper represents the characteristics of evapotranspiration rate (EVTR) and graft-taking of watermelon grafted seedlings in a graft-taking enhancement system using fluorescent lamps as artificial lighting source. Four air temperature levels of 23, 25, 27 and 29C, three humidity levels of 85, 90 and 95%R.H. and two photosynthetic photon flux (PPF) levels of 30 and 50 ${\mu}$mol m$\^$-2/ S$\^$-1/ were provided to investigate the effects of air temperature, relative humidity and light intensity on EVTR and graft-taking of grafted seedlings. EVTR of grafted seedlings increased with increasing air temperature and the passage of time after grafting. Also EVTR increased with decreasing relative humidity. As relative humidity decreased and air temperature increased, vapor pressure deficit increased and thus EVTR increased. It is required to maintain a low level vapor pressure deficit for suppressing EVTR of grafted seedlings during first 1-2 days after grafting. Therefore, less EVTR at initial stage after grafting would be adequate for smooth joining of the scion and rootstock.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.18-22
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• 2002

Diseases occurrence of hydroponic culture was less than that of soil culture, especially gummy stem blight, which was 39.9-53.3% of infection rate at soil culture, was not almost observed at hydroponic culture. Development of fermented fruit was higher at Shintozoa, Hongtozoa and self-rooting seedling in order at soil cultured but critically dropped at hydroponic culture without rootstock sort. Therefore proper moisture management at root zone can decrease the development of fermented fruit.

• Journal of Forest and Environmental Science
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• v.34 no.1
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• pp.77-81
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• 2018

Mass production of forest medicinal plants is related to quality control of raw medicinal materials. Plant tissue culture is an important technology to produce high-quality plant materials. Numerous factors are reported to influence the success of in vitro regeneration of medicinal plants. Embryogenesis is known to be the most effective techniques and it has developed in some medicinal plant species. Various in vitro cultural condition for direct and/or indirect somatic embryogenesis systems have developed in Epimedium koreaum, Bupleurum falcatum, Paeonia lactiflora, Chrysanthemum zawadskii, Houttuynia cordata etc. In this study, we provide the present statue and information of in vitro propagation techniques that is able to apply as an efficient system for rootstock propagation system of forest medicinal plants.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.408-408
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• 2005

• Journal of Biosystems Engineering
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• v.31 no.5 s.118
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• pp.449-453
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• 2006

Effect of light quality on plant temperatures at joining parts of grafted watermelon seedlings was investigated using a thermal imaging system in this study. Plant temperatures at joining parts lowered than those at stem region during graft-taking. However, difference in plant temperatures at joining parts and at stem region decreased by days after graft-taking. Plant temperatures of grafted seedlings graft-taken under white, blue, red, and blue + red fluorescent lamps repeatedly fluctuated high at photoperiod and low at dark period. Considering the variation of plant temperatures at joining pa퍼5, the illumination of blue and red light as well as white light will be effective for grafting of grafted watermelon seedlings. It is recommended that air temperature around grafted seedlings should be maintained at 27 to $29^{\circ}C$ for enhancing the physiological reaction of callus and for the smooth joining of scion and rootstock.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.215-215
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• 2004