• 생물환경조절학회지
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• 제18권4호
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• pp.393-400
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• 2009

적심 및 rootone 처리가 고추 10품종의 발근력에 미치는 영향을 조사한 결과, 모두 정상적으로 발근되었으나 품종간에 큰 차이를 보였다. 즉 'Gukbo'와 'Chungyang' 등은 발근력이 낮은 반면, 대목 이용 가능성이 높은 'Geumsang' 등은 매우 높았다. 발근촉진제 rootone 처리는 모든 품종의 발근을 현저히 증가시켜 높은 실용성을 보여주었다. 또한 적심에 따른 발근 지연 현상은 rootone 처리로 거의 완벽하게 보완될 수 있었다. 선발된 대목 10품종과 접수 3품종을 적심과 IBA 단용 또는 병행처리 후 발근력을 검토한 결과, 대목 중에서는 'Tantan'이 가장 높았고, 'Konesian Hot'과 'Wonkwang 1호'도 높았다. 또한 공시된 10개 대목은 대면적에 일반재배 되는 품종인 'Manita', 'Chungyang', 'Nokkwang'보다 발근력이 높았다. IBA 처리는 발근력을 다소 향상시켰으나 rootone 처리에 비해 효과가 뚜렷하지 않았다.

• Journal of Biosystems Engineering
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• 제32권3호
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• pp.179-189
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• 2007

Most of the seeds of cucurbitaceous rootstock species used for grafting were mainly sown by hand. This study was carried out to develop an on-line discriminating algorithm of seed direction using machine vision and an automatic seeding system. The seeding system was composed of a supplying device, feeding device, machine vision system, reversing device, seeding device and system control section. Machine vision was composed of a color CCD camera, frame grabber, image inspection chamber, lighting and personal computer. The seed image was segmented into a region of seed part and background part using thresholding technique in which H value of HSI color coordinate system. A seed direction was discriminated by comparing position between the center of circumscribed rectangle to a seed and the center of seed image. It took about 49ms to identify and redirect seed. Line-up status of seed was good the more than 95% of a sowed seed. Seeding capacity of this system was shown to be 10,140 grains per hour, which is three times faster than that of a typical worker.

• 식물병연구
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• 제16권3호
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• pp.290-293
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• 2010

국내에서 Clavibacter michiganensis subsp. michiganensis에 의한 궤양병은 토마토 농가에 심각한 피해를 주고 있다. 이에 병 발생의 감소와 방제를 위해서는 저항성 품종이 최선의 해결책으로 제시되고 있다. 따라서 국내에서 시판되고 있는 완숙토마토 39품종, 방울토마토 20품종, 대목토마토 13품종에 대한 궤양병 저항성 품종을 선별하기 위해 저항성 검정을 실시하였다. 시판되는 72품종 중 선명, 스위트, 다다끼, 아기꼬, 매치, 마그네트, 프렌즈, 그린파워, 품종이 궤양병에 대해 저항성을 나타내었다.

• 한국약용작물학회지
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• 제7권3호
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• pp.213-217
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• 1999

뿌리에 의한 영양번식으로 증식하는 지황은 바이러스에 심하게 삼염되어있어 종자를 이용한 우량종근을 생산할 목적으로 종자특성과 발아에 미치는 요인과 알진산으로 인공피막한 처리를 검토한 결과는 다음과 같다. 지황종자의 특성으로 협당종자수는 서천재래 61개에 비하여 지황1호가 207개로 많았고 1,000립중도 서천재래 70mg에 비하여 지황1호가 130mg으로 무거웠다. 지황종자는 당년채종('98)한 종자보다 전년도 채종 후 상온 저장한 종자에서 높은 발아율을 보여 미세립 종자지만 휴면현상이 있었고 온도 차이에 따른 발아는 차이를 보여 $15^{\circ}C$에서는 9일까지 발아하지 않았으나, $30^{\circ}C$에서는 2일후 28.5%, 6일후 41.6%로 높았다. 종자파종의 생력화를 목적으로 지황종자를 알진산 피막처리 하였을 때 파종후 20일에 발아율이 83.2% 정도였다. 알진산 처리농도는 2%의 저농도에서 발아율이 높았고 인공피막내 함유 종자수는 3개이상까지 많을수록 양호하였으며 저온처리는 초기발아가 지연되었다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.63-69
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• 2010

The effect of Acibenzolar-S-methyl (ASM) and Rahnella aquatilis Ra39 against apple fire blight disease caused by Erwinia amylovora were tested as a possible alternative to streptomycin. In vitro studies, no inhibition effect against the pathogen was found when ASM was tested. Under greenhouse conditions, application of R. aquatilis Ra39 with the highly susceptible M26 rootstock resulted in a marked disease suppression. Application of ASM and strain Ra39 caused a high decrease of the disease, 82% and 58% respectively; this was correlated with a reduction of the growth of the pathogen within host plants up to 64% and 49.5% respectively. Further studies in the field under artificial infection condition during full bloom revealed that application of ASM and R. aquatilis Ra39 with Gala variety resulted in a control effect up to 21 and 29% respectively. In physiological studies, enhanced activities of PR-proteins (chitinase and $\beta$-1, 3-glucanase) were detected, which are well known as biochemical markers for systemic acquired resistance. Application of ASM to apple shoots caused the highest chitinase activity followed by strain Ra39. The enzyme activity was increased after 2, 4 and 6 days from application. In addition, ASM-treatment caused the higher $\beta$-1, 3-glucanase activity than strain Ra39. Maximum enzyme activity was recorded after 6 days from application and then decreased after 8 and 10 days from application.

• 한국작물학회지
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• 제48권3호
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• pp.152-155
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• 2003

야생의 안동대목은 종자의 크기 및 충실도에 많은 변이를 보이고 있다. 본 연구는 약용 및 박과접목묘의 대목용으로 이용되고 있는 안동대목 종자의 크기 및 성숙 정도가 발아, 유묘 출현 및 생장에 미치는 영향을 조사하기 위하여 실시되었던 바 그 결과를 요약하면 다음과 같다. 안동대목 종자의 발아율 및 유묘출현율은 미성숙 종자에 비하여 성숙 종자에서 높았다. 그러나 종자의 크기에 따른 발아율은 성숙 종자와 미성숙 종자 모두 대립종자에 비하여 중립 또는 소립종자에서 높았던 반면, 유묘출현율은 중립종자에서 가장 높고 대립종자에서 가장 낮았다. 종자 크기와 성숙 정도가 유묘 형태 및 생장에 미치는 영향으로는 하배축 길이를 제외한 초장, 본엽수, 자엽 및 본엽의 면적은 종자 크기가 클수록 길고 많았던 반면, 종자의 성숙 정도간에는 차이가 없었다. 그러나 각 부위 및 전체 건물중은 미성숙 대립종자보다는 성숙 대립종자에서, 성숙 유무에 관계없이 종자의 크기가 클수록 많았다

• 식물조직배양학회지
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• 제28권1호
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• pp.33-36
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• 2001

고밀도 저수고형의 성공적인 왜화재배를 위한 사과 왜성대목인 M.9 T-337의 대량증식체계를 확립하기 위하여 실험한 결과를 요약하면 다음과 같다. 초기 생장점 배양은 채취한 경정조직을 ascorbic acid와 citric acid를 각각 100 mgL$^{-1}$와 150mgL$^{-1}$로 혼합한 용액에 30분간 전처리한 다음, IBA 0.1 mgL$^{-1}$, GA 0.5 mgL$^{-1}$와 sucrose 30 gL$^{-1}$가 첨가된 MS 액체배지에서 50 rpm으로 2주간 진탕배양한 후, 한천 8 gL$^{-1}$가 첨가된 동일조성의 MS 배지에서 배양하는 것이 신초형성에 가장 좋았다. 발근은 증식된 신초를 1주일간 Hormex 1,000배 액이 첨가된 1/2 MS 기본배지에 IBA 0.5 mgL$^{-1}$, sucrose 30 gL$^{-1}$ 등이 함유된 배지에서 50 rpm으로 진탕배양한 다음 Hormex를 제외한 동일 조성의 한천배지에 옮겨 배양하였을 때 배양된 모든 유묘로부터 정상적인 발근을 유도할 수 있었다.

• 생명과학회지
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• 제11권2호
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• pp.166-172
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• 2001

Various types of the seeding of bottle gourd widely used as a rootstock of watermelon has been required to satisfy the farmers need. The study was done to determine the effect of light quality of blue, red and far-red lights treated with light emitting diodes on growth and morphology of bottle goured seeding and chlorophyll content of its cotyledons. The lights were treated in the growth chamber for 7 days to the seeding elapsed 8 days after sowing under natural condition, and 64 hole trays with commercial bedsoil. Plant height, length and diameter of hypocotyl, leaf area of cotyledon and first true leaf, its leaf length, number of true leaves, fraction and total dry weight were measured. Red light shortened and slenderized the hypocotyl, which lengthened by far-red light and thickened by blue light. Plant height was declined in order of far-red light treatment, blue and red lights mainly due to difference of hypocotyl length, Area and length of the first true leaf became smaller and shorter under far-red light than under the other lights. However, blue light increased leaf area of cotyledons. Two cultivars cv. Yongjadaemok and cv. Kunghap had different response to the light treatments in total seedling dry weight(dw); far-red and red light treatments showed the greatest and the least dw of hypocotyl, respectively, while blue and red lights did the greatest dw of the other organs. Among the ratio of each organ dw to total dw, those of hypocotyl and true leaves were different between the light treatments; the highest ratio of hypocotyl dw to total dw was observed in far-red light treatment but the lowest was in red light treatment. Those of the true leaves were the lowest in far-red light and similar response in blue or red light treatment. Chlorophyll content of cotyledons was decreased in order of red light treatment, blue and far-red lights, meaning that short period light treatment may influence photosynthesis of seeding and afterward its growth.

• Journal of Plant Biotechnology
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• 제46권4호
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• pp.274-281
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• 2019

Pistacia chinensis Bunge has not only been used as a medicinal plant to treat various illnesses but its young shoots and leaves have also been used as vegetables. In addition, P. chinensis is used as a rootstock for Pistacia vera (pistachio). Here, the transcriptome of P. chinensis was sequenced to enrich genetic resources and identify secondary metabolite biosynthetic pathways using Illumina RNA-seq methods. De novo assembly resulted in 18,524 unigenes with an average length of 873 bp from 19 million RNA-seq reads. A Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation tool assigned KO (KEGG orthology) numbers to 6,553 (36.2%) unigenes, among which 4,061 unigenes were mapped into 391 different metabolic pathways. For terpenoid backbone and carotenoid biosynthesis pathways, 44 and 22 unigenes encode enzymes corresponding to 30 and 16 entries, respectively. Twenty-two unigenes encode proteins for 16 entries of the carotenoid biosynthesis pathway. As for the phenylpropanoid and flavonoid biosynthesis pathways, 63 and 24 unigenes were homologous to 17 and 14 entry proteins, respectively. Mining of simple sequence repeat identified 2,599 simple sequence repeats from P. chinensis unigenes. The results of the present study provide a valuable resource for in-depth studies on comparative and functional genomics to unravel the underlying mechanisms of the medicinal properties of Pistacia L.

• Plant Biotechnology Reports
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• 제2권2호
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• pp.137-143
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• 2008

Production of Indian citrus ringspot virus (ICRSV)-free plants from an infected plant of kinnow mandarin (Citrus nobilis Lour ${\times}$ C. deliciosa Tenora) is reported. The shoot apices of different sizes (0.2-1.0 mm) excised from the ICRSV-infected plant were micrografted onto decapitated rootstock seedlings of rough lemon (C. jambhiri). Micrograft survival depended on the size of shoot apex and the sucrose concentration of the culture medium. Increase in scion size from 0.2 to 0.7 mm resulted in an increase in micrografting success rate from 30.55 to 51.88%. Further, micrograft survival obtained with 0.2 mm was improved from 30.55 to 38.88% by increasing sucrose concentration in the culture media from 5 to 7.5%. The micrografted plants were tested for ICRSV using ELISA and RT-PCR. All plants raised from 0.2-mm scion were found negative with both ELISA and RT-PCR whereas only 20% of the ELISA negative plants raised from 0.3-mm scion were found negative for ICRSV with RT-PCR. The outcome of this research is the successful establishment, acclimatization and virus testing of micrografted plants.