• Title/Summary/Keyword: Roots Method

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Extraction of Low Molecular Weight Ginsenosides from Adventitious Roots Culture of Wild Mountain Ginseng by Steam Processing (증숙 처리에 의한 산삼 부정 배양근의 저분자 진세노사이드 추출)

  • Lee, Ye Ji;Kim, Hee Kyu;Go, Eun Ji;Choi, Jae Hoo;Jo, Ah Reum;Kim, Chul Joong;Lee, Jae Geun;Lim, Jung Dae;Choi, Seon Kang;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.2
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    • pp.148-156
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    • 2018
  • Background: Hot steaming is known to be effective in improving the biological activities of plant extracts by breaking down useful compounds to low molecular weight ones. Methods and Results: This study aimed to develop an optimal extraction and steam processing method for enhancing the low molecular ginsenoside contents of the adventitious roots culture of wild mountain ginseng. The total ginsenoside was optimally extracted when 70% EtOH was used at $50^{\circ}C$, whereas low molecule ginsenoside such as Rg2, Rh1, Rh4 and Rk1 could be extracted using 70% EtOH at $70^{\circ}C$. The adventitious roots culture of wild mountain ginseng is known to contain four major ginsenosides, i.e., Rb2, Rb1, Rg1 and Rd, however new ginsenosides Rg6, Rh4, Rg3, Rk1 and Rg5 were new abundantly obtaind after steam processing method was applied. The contents of total ginsenosides were the highest when thermal steam processing was conducted at $120^{\circ}C$ for 120 min. Unlike ginsenosides such as Rg1, Re, Rb1, Rc, Rb2, and Rh1, which decreased after steam processing, Rg3, Rk1, and Rg5 increased after thermal processing. Steam processing significanltly reduced the content of Rb1, increased that of Rg6 by about ten times than that in the adventitious roots culture of wild mountain ginseng. Conclusions: Our study showed that the optimal extraction and steam processing method increased the content of total ginsenosides and allowed the extraction of minor ginsenosides from major ones.

A Study on New Working System of Machanical Land Clearing and Development of Rertile Soil (기계 개간의 새로운 작업 체계와 숙지화 촉진에 관한 연구)

  • Hwang, Eun
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.14 no.1
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    • pp.2535-2548
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    • 1972
  • Instead of fertilizing the lands they moved to find other fertile lands and lived a feudal life agriculture and various machincs played a main role in the land reclamation. The best method of land clearing, the time and capacity in the operation and the effect of growing crops should systematically analized prior to the time of 3rd Five-year Economic Development(1972-1976) in order to cultivate 210,000ha of wasteland or the modernization of our country. The present study was investigated to find out a new working-system of mechanical land clearing and development of fertile soil. The results are as follows: 1) The first stage of productive power increases when weeds were mixed soil in the land clearing work in order to supply organic materials and to make soil swollen instead of burning or just removing. 2) The equipments such as bulldozers, harrows, power tillers and so on should be prepared in order to do a systematic work in the land clearing. 3) The work of pulling-up roots is dependent upon the forms of roots spreading under the ground. The work of the pulling-up of the straight roots was most difficult. But before the roots are pulling-up we do not know the forms of roots. 4) The cutting of soil from upside to under side land clearing method (II) Spent 6 hr 7 min 43 sec per 1 Danbo in doing land clearing and control method spent 4 hr 52 min 30 sec per 1 Danbo in doing land cearing. No significant difference could be found between II treatment type and I treatment type. 5) The volume of soil carried by bulldozer $270.38m^3$ per 1 Danbo in I type and in II type, $368.58m^3$ per 1 Danbo or about 36% increased in II type then in I type. But that is not a significant difference when considering the whole productive power of soil. 6) The land clearing of terrace by bulldozers cannot escape making the embankment slope of about $45^{\circ}$, and 25.3% should be decreased in the area. It is recommended to make use of the embankment slope by planting grass for cows. 7) The time of retary tilling increased 2.3 times or 1 hr 42 min 22 sec per 1 Danbo compared to the timesrequired in the harrowing. Because it mixed the Organic matter on ground and the harrowing of subsoil. 8) The havest of pasture growing on the land reclamation in natural slope is as follows. (a) The none fertilizers with reclaimed block of productivity decresed 1/10 than that of the farmland. Therefore the none fer tilizing in the cultivated pasture is unprofitable. (b) The havest of the manured IV treatment block was the best not only in the kinds of fertilizers but also in the kinds of pasture grass.

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A Comparison between Suanxue qimeng(Introduction to Mathematical Studies} and Muksa-jipsanbup (산학계몽과 묵사집산법의 비교)

  • Her, Min
    • Journal for History of Mathematics
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    • v.21 no.1
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    • pp.1-16
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    • 2008
  • Suanxue qimeng(算學啓蒙) is the introduction to mathematics which greatly influenced Chosun mathematics, Muksa-jipsanbup(默思集算法) imitated the style and the contents of Suanxue qimeng, but contains a lot of problems, secondary solutions and topics which is not in Suanxue qimeng and tried to achieve educational improvement. However Muksa-jipsanbup could not use the method of rectangular arrays(方程術) because it excluded the method of positive and negative(正負術), and has a serious limitation in applying the method of extracting roots by iterated multiplication(增乘開方法) because it avoided the technique of the celestial element(天元術).

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Distribution of Stem Vestige according to Ginseng Cultivars and Determination of Root Age by Ginsenoside Types of Red Ginseng (인삼 품종별 경흔적 분포 및 홍삼의 진세노사이드 종류에 따른 연근 판별)

  • Kim, Young Chang;Kim, Jang Uk;Bae, Bong Suk;Kang, Je Yong;Kim, Dong Hwi;Hyun, Dong Yun;Lee, Joon Soo
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.4
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    • pp.217-223
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    • 2017
  • Background: In Korea, 6-year-old ginseng root is economically more important than 4 or 5-year-old roots. In general, the root age is determined by counting the number of stem vestiges. However, this method does not accurately estimate ginseng root age. Methods and Results: In this study, the stem vestige counting method was used to survey a total of 18,395 fresh ginsengs cultured in 2014, and 2015, to determine the accuracy of this method. The proportion of 6-year-old roots, with more than four stem vestiges, was 46.1% in 2014. For the cultivar Chunpoong cultivated in Eumseong and Goesan countries in 2015, the proportion of more than four stem vestiges was 55.9%, and 43.5%, respectively. The proportion of more than four stem vestiges for the Gumpoong cultivated in Eumseong and Yangpyeong countries was 67.0%, and 35.1%, respectively, whereas that for the cultivar Yunpoong was 36.0% and 61.0%, respectively. Moreover, it was confirmed that differences in the levels of Rg1 will enable root age determination. Conclusions: Root age determination by the stem vestige test was found to differ depending on the environmental and cultivation conditions. To determine the age of ginseng roots, a comprehensive method, such as counting stem vestiges and evaluating differences in ginsenoside levels, should be applied.

Local Modification of a Surface and Multiple Knot Insertion by Using the Chebyshev Polynormial (Chebyshev 다항식에 기초한 다수개의 절점 삽입과 곡면의 국부 수정)

  • 최성일;김태규;변문현
    • Korean Journal of Computational Design and Engineering
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    • v.3 no.2
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    • pp.103-112
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    • 1998
  • In this paper insertion of numerous control points to be performed by using the Chebyshev polynomial root at the selection of knot vector. This method introduces a simple method of knot refinement and it is applied in a developed program. The Chebyshev roots exist densely in broth ends of the range and are proposed more effective knot refinement to modify a surface. Therefore, generated control points are relatively uniform in specified knot interval. In the surface generation, a local insertion of numerous control points are easily inserted by using the characteristic of Chebyshev polynomial roots at knot refinement. It is possible to create a complex surface with a single surface. The number of control point can be reduced by using the local insertion of control points in a required shape

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Scanning Electron Microscope Observations in the Apices of Roots with Refractory Apical Periodontitis.

  • Matsuo, T.;Fujinaka, K.;Yoshida, Y.;Ozaki, K.
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.561.1-561
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    • 2001
  • The aim of this study was to examine the localization of bacteria in the apices of roots with refractory apical periodontitis by a scanning electron micrscopy(SEM), and to identify the extra-radical bacteria using a immunohistological method. Eleven teeth were prepare for immunostaining and 4 for SEM. Immunostaining was performed with LSAB method and specific antisera against 18 bacteria selected for this study. Specimens for SEM observations were dried using a t-butyl alcohol freeze dryer and coated with gold-palladium.(omitted)

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The Pharmacognostical Studies of Angelica Plants in Korea (II) A Classification Method of the Roots of some Anegelica Genus Plants by Thin Layer Chromatography (한국산 anglica 속식물의 생약학적연구 (II) 수종 Angelica 속식물근의 Thin Layer Chromatography를 이용한 감별법에 대하여)

  • 정보섭;지형준
    • YAKHAK HOEJI
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    • v.11 no.3_4
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    • pp.33-35
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    • 1967
  • The internal structures of "Chunho", "Kangwhal" and "Dangui" and their original plants had been compared with each other in the prior report. Coumarin derivatives, khellactone from the roots of Angelica purpuraefolia $C_{HUNG}$ and decursin and decursinol from A. gigas $N_{AKAI}$ were also isolated. In this report, we made a simple and convenient identification method for the powdered drugs of several Angelica genus by using a thin layer chromatogrophic technique. The ehter extract of the powdered drugs were applied to a silica gel plate, and was developed with n-hexane and ethyl acetate (1:1) mixture. The characteristic U.V. absorption patterns of each drug were shown.

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Quality Control of Adenophorae Radix

  • Park, Yun-Kyung;Yoo, Hye-Hyun;Baek, Seung-Hoon;Lee, Seung-Ho;Kim, Chang-Min;Lee, Kyung-Soon;Park, Man-Ki;Park, Jeong-Hill
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.221.3-222
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    • 2003
  • Adenophorae Radix, the roots of Adenophora triphylla var. japonica Hara (Campanulaceae) is known to be an anti-inflammatory and antitussive drug used for the treatment of lung disease. However quality control method is not established yet. This study is to establish the quality control method of Adenophorae Radix.From the roots of this plant, we isolated (6R,7R)-E,E-tetradeca-4,12-diene-8,10-diyne-1,6,7-triol (1) as a marker compound for the quality control and determined its content by HPLC-UV detector. (omitted)

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History of solving polynomial equation by paper folding (종이접기를 활용한 방정식 풀이의 역사)

  • CHOI Jaeung;AHN Jeaman
    • Journal for History of Mathematics
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    • v.36 no.1
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    • pp.1-17
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    • 2023
  • Paper folding is a versatile tool that can be used not only as a mathematical model for analyzing the geometric properties of plane and spatial figures but also as a visual method for finding the real roots of polynomial equations. The historical evolution of origami's geometric and algebraic techniques has led to the discovery of definitions and properties that can enhance one's cognitive understanding of mathematical concepts and generate mathematical interest and motivation on an emotional level. This paper aims to examine the history of origami geometry, the utilization of origami for solving polynomial equations, and the process of determining the real roots of quadratic, cubic, and quartic equations through origami techniques.

Development of Effective Screening Method and Evaluation of Radish Cultivars for Resistance to Fusarium oxysporum f. sp. raphani (효율적인 무 시들음병 저항성 검정법 개발 및 무 품종들의 병 저항성 평가)

  • Baik, Song-Yi;Kim, Jin-Cheol;Jang, Kyoung-Soo;Choi, Yong-Ho;Choi, Gyung-Ja
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.148-152
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    • 2010
  • To establish the efficient screening method for resistance of radish to F. oxysporum f. sp. raphani, we investigated the development of Fusarium wilt of two radish cultivars, 'Songbaek' (susceptible) and 'Tokwang' (moderately resistant), according to several conditions such as inoculation methods, inoculum concentrations, and dipping periods of radish roots in spore suspension. By infected soil and soil-drenching inoculation methods, Fusarium wilt did not occur on the seedlings of both cultivars. In root dipping inoculation method using cut or non-cut roots of radish plants, the cut roots were easily infected by the pathogen than non-cut roots. And the disease development of two cultivars represented significant difference in non-cut root method. On the other hand, disease severity of Fusarium wilt on radish seedlings according to inoculum concentration increased in a dose-dependant manner, regardless of dipping periods. Using screening method established from the results, the 41 commercial radish cultivars were evaluated the degree of resistance to F. oxysporum f. sp. raphani. Among them, 6 radish cultivars were resistant, 22 cultivars were moderately resistant, and 13 cultivars were susceptible to Fusarium wilt.