• Title/Summary/Keyword: Rooting of cuttings

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Effective Cutting Length and NAA Concentration for Water Cutting Propagation in Clematis (클레마티스 수삽번식 향상을 위한 삽수길이 및 NAA 농도)

  • Kil, Mi Jung;Yoo, Bong Sik;Kwon, Young Soon;Choi, Seong Youl
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.288-292
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    • 2014
  • This study was performed to investigate the possibility of water cutting by vegetative propagation method from July to August of Clematis. To find out proper cutting length and rooting growth regulator for water cuttings production, cutting lengths of C. 'Honora' made 1 node and 2 nodes length cutting, which were soaked in NAA $0.1mg{\cdot}L^{-1}$. Rooting rate of cuttings treated by NAA $0.1mg{\cdot}L^{-1}$ was 100% irrespective of root lengths, but that of 1 node and 2 nodes untreated by NAA $0.1mg{\cdot}L^{-1}$ were very low about 30% and 19% respectively. Number of roots and roots length were also highest in NAA $0.1mg{\cdot}L^{-1}$, effects of NAA $0.1mg{\cdot}L^{-1}$ treatment were greater at 1 node cutting. Based on the results, rooting rate for I node cutting of C. 'Pairu' according to NAA (0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) concentrations was investigated. Days to rooting were 70-74 days (about 10 weeks), which was not significantly different among the NAA concentrations. Rooting rate increased by NAA concentration. it was about 90% usually and was improved with more than NAA $0.5mg{\cdot}L^{-1}$.

Soil Moisture Content Affecting Rooting of Cutting and Height Growth of Populus alba × P. glandulosa and Parents (토양수분(土壤水分)이 Populus alba × P. glandulosa 및 교배양친수(交配兩親樹)의 삽목발근(揷木發根)과 생장(生長)에 미치는 영향(影響))

  • Son, Doo Sik;Shin, Jong Bok
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.74-78
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    • 1984
  • The highest cutting ratio and the best growth performance of the P. alba, P. alba ${\times}$ P. glandulosa and P. glandulosa were at -0.05 bars of soil water potential (${\Psi}_s$). Their growth and rooting ability of cuttings were better in suitable soil moisture contents, 19.650, but were worse in low soil moisture contents, 11.41% and 16.66%, respectively. Specially, P. alba ${\times}$ P. glandulosa appeared hybrid vigor in high soil water potential (${\Psi}_s$= -0.05 bars) but not appeared it below ${\Psi}_s$ = -0.5 bars. It is believed that P. glandulosa is a natural hybrid between P. davidiana and P. alba in view of morphological characteristics. Aspen, normally, does not have the rooting ability of cuttings, however, P. glandulosa showed the rooting ability, though the ratio was low, 23%. It is estimated that this rooting ability was originated from P. alba. In addition, its growth rate was between P. alba and P. davidiana, and the sensitivity of water requirement for the growth was also really equal to P. alba. This facts prove P. glandulosa to be natural hybrid between P. alba and P. davidiana.

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Effect of Plug Cell Volume and Medium Composition on Rooting and Growth of Lateral Shoot Cuttings of Tomato Plant (플러그 셀의 크기와 배지(培地) 조성이 토마토 삽목묘(揷木苗)의 발근(發根)과 생육(生育)에 미치는 영향(影響))

  • Yang, Seung-Koo;Cho, Myeong-Su;Choe, Kyeong-Ju;Kim, Wol-Soo
    • Horticultural Science & Technology
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    • v.19 no.3
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    • pp.325-328
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    • 2001
  • This experiment was conducted to investigate the effect of plug cell volume and medium on rooting and growth of lateral shoot cuttings of a few tomato cultivars. Plug cell volume was varied from 23 to 300 mL or control (cutting bed) and media used were carbonized rice hull (CRH), CRH+perlite, CRH+peatmoss and perlite+peatmoss. Nursery plants were able to be transplanted in 15 to 20 days after lateral shoots cutting in tomato. In volume of cutting media, the pots of 23 to 300 mL were proper, although root growth was gradually limited in decreased media volume. Rooting and growth was not influenced by plug tray cell medium of 120 mL or more. Cherry tomato Pepe (c.v.) showed 100% rooting and better growth, while in Momotaro (c.v.) rooting was 90%. There were 100% rooting and no considerable changes grown in all media tested of cutting plug tray volume of 30 mL.

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Effects of Cutting Date, Shading Ratio and Growth Regulator on Rooting of Acanthopanax divaricatus var. albeofructus and Acanthopanax koreanum Nakai (흰털오갈피나무와 섬오갈피나무의 삽목시기, 차광율 및 생장조절제 처리가 발근에 미치는 영향)

  • Lee, Jung-Jong;Lee, Sang-Hyun;Seo, Pil-Dae;Park, Chun-Geun;Lee, Sang-Chul
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.5
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    • pp.353-358
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    • 2012
  • This study was conducted to enhance the propagation of Acanthopanax divaricatus and Acanthopanax koreanum through different cutting practices. For propagation by cuttings, scions were taken on September 1. This already has hard skin and showed much higher values in terms of root length, root number, rooting ratio and root weight than those taken on June 30 and August 1. Regardless of cutting date, 50% shading resulted to the highest root length, root number, root ratio and root weight. Meanwhile, 95% shading significantly reduced these parameters compared with no shading. These results suggest that over-shading may inhibit root growth. Two growth regulators, IBA (concentration of 1,000, 2,000, 3,000 ppm) and Rootone-F (0.4%) were also tested of its effect to the cuttings. Rootone-F was found to be more effective than IBA. Cutting treated with Rootone-F had slightly higher root length and root number. A. koreanum which grows well in hot climatic condition showed better rooting ability than A. divaricatus.

Micropropagation Using Shoot Tip Culture of Pyrus ussuriensis Maximowicz (정아배양에 의한 산돌배나무의 기내번식)

  • Moon, Heung-Kyu;Lee, Sung-Jae
    • Journal of Korean Society of Forest Science
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    • v.97 no.4
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    • pp.452-457
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    • 2008
  • In order to develop an efficient micropropagation technique effect of plant growth regulators (PGRs) affecting on shoot proliferation from shoot apex in Pyrus ussuriensis was tested. Generally, there was no conspicuous effect on shoot induction by the treatment of PGRs and one or two shoots/explant were induced when cultured on MS medium supplemented with BA and/or BA plus NAA. Both apical shoot necrosis and hyperhydric shoots were observed frequently in multiplied shoots, and callus was formed at the basal part of shoots. About 20% spontaneous rooting was achieved in growing shoots, however the proliferated shoots exhibited poor rooting rate in gelrite supported media. When we tried to ex vitro rooting of the shoot cutting, the shoot cuttings rooted up to 50% with 100 mg/L IBA application. The rooted plantlets grew normally after acclimatization in the greenhouse.

Propagation of a New Anticancer Plant, Cephalotaxus koreana, by Cutting (신규 항암소재의 개비자나무의 삽목증식)

  • Jung, Myung-Suk;Hyun, Jung-Oh;Lee, Uk;Fadly Yahya, Andi;Baik, Eul-Sun
    • Journal of Korean Society of Forest Science
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    • v.98 no.4
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    • pp.491-495
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    • 2009
  • This study was conducted to investigate the propagation characteristics of Cephalotaxus koreana by cutting, whose plant has been known to new anticancer treatments. The treatments of plant growth regulators, cutting media temperature and growing media were investigated to know the rooting ability of cuttings. For rooting formation affected by plant growth regulators and its concentration, The application of IBA 100 mg/L was most effective in all of the characteristics including rooting rate of 88.5%. The optimum media temperature by cutting was $24^{\circ}C$ (treatment IBA 100 mg/L, rooting rate 84.6%), predicting that greenwood cutting from June to July is most effective. VPPL (vermiculite+peatmoss+perlite 1:1:1(v/v/v)) showed the highest value in all of the characteristics including rooting rate of 68.5% for the effect of growing media on rooting formation.

In Vivo and In Vitro Rooting of Rehmannia glutinosa Plantlet Regenerated in Vitro (기내증식된 지황묘의 기내 및 기외 발근)

  • 백기엽;유광진;박상일
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.6
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    • pp.335-340
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    • 1997
  • 100% root formation in in vitro cultures was observed regardless of kind and levels of auxin used and explant source. The number of roots/explant was increased in 0.5~1.0 mg/L IAA treatment. Thicker roots were observed with the addition of 9% sucrose compared with medium containing lower sucrose concentrations. Paclobutrazol and chlormequat had no effect on tuberization of formed roots but slightly increased the number of root. In in vivo rooting, soaking of regenerated shoot cuttings to 100 mg/L IBA for 15 to 60 minutes was found effective. Treatment of 0.1% IBA rooting powder and planting in rooting medium composred of vermiculite(1) : perlite(1) gave 100% rooting and survival.

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The Effect of Cutting Positions and Temperature on the Rooting of Bitter Gourd (여주 삽목 시 삽수절단 위치와 온도조건이 발근에 미치는 영향)

  • Lee, Hee Ju;Lee, Sang Gyu;Kim, Sung Kyeom;Choi, Chang Sun;Kim, Sang Beom
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.162-167
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    • 2016
  • This study was carried out to investigate proper stem cutting position and air temperature to regenerate roots from cuttings of bitter gourd. 'NS454' (NS) and 'Dragon' (DR) cultivars were tested and the cutting position was cut at the 3rd node in the stem (treatment I) and cut at the central part (stem segment) between 3rd leaf and 4th leaf in the stem (treatment II). The air temperature was maintained at 18, 23, 28 and $35^{\circ}C$ in the growth chambers, respectively. The photosynthetic photon flux at the ground of a chamber was maintained in approximately $150-200{\mu}mol\;m^{-2}s^{-1}$ during the 16-hour photoperiod. The relative humidity in the chambers was maintained over 85%. After 10 days of cuttings, regardless of cutting position, DR and NS cultivars showed 100% the survival rate in the $18^{\circ}C$ or $23^{\circ}C$ treatments, however, most of plants $18^{\circ}C$ treatment had not rooting. In the $28^{\circ}C$ treatment, regardless of cutting position, DR and NS cultivars showed 100% of the rooting rate. In the $23^{\circ}C$ treatment, for DR cultivar, the node cutting showed 90% of rooting rate and the stem segment cutting showed 40% rooting rate. For NS cultivar, the node cutting showed 50% of rooting rate and the stem segment cutting showed 40% rooting rate. DR and NS cultivars have high rooting rate, while the rooting rate remarkably decreased in the $35^{\circ}C$ treatment. For DR cultivar, the stem segment cutting showed 5.3 of the highest rooting number and the node cutting showed 2.7 rooting number in the $28^{\circ}C$ treatment. NS cultivar was not significantly different the rooting regardless of cutting position. The results suggested that cutting at the stem segment cutting of bitter gourd is an effective cutting method for increasing survival rate and the air temperature should be maintained at $28^{\circ}C$.

Rooting Rate and Survival Rate as Affected by Humidification Period and Medium Type of 'Maehyang' Strawberry on Cutting Propagation ('매향' 딸기의 삽목 번식 시 가습 기간 및 배지 종류에 따른 발근율과 생존율)

  • Hwang, Hee Sung;Jeong, Hyeon Woo;Lee, Hye Ri;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.29 no.3
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    • pp.219-230
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    • 2020
  • This study was conducted to determine the optimum medium and humidification period for the strawberry (Fragaria × ananassa Duch. cv. Maehyang) cutting seedling production. The cuttings were planted in coir (CO), rockwool (RW), phenolic foam (PF), and organic foam medium (OFM) with fogging treatment in 0, 3, 6, 9, or 12 days on February, 2019. And, as the field verification examination research, cuttings were planted in CO, RW, PF, and OFM, with misting treatment in 0, 6, 9, 12, or 15 days on June, 2019. In fogging treatments, rooting and survival rates of seedlings tended to increase with longer fogging periods, and rooting and survival rates were showed significantly higher in CO medium with more than 9 days of fogging periods. In misting treatments, rooting rate was significantly higher in CO and RW medium with more than 9 days of misting periods, and survival rate was significantly higher with more than 12 days of misting periods. There was no significantly difference by growth medium and humidification period in shoot and root growth. Therefore, when considering the rooting and survival rate the CO medium with 9 days of humidification period could be beneficial for the production of the 'Maehyang' strawberry cutting seedlings.

Effects of Cutting Condition on Rooting and Growth of Cut Flower in Plug Cutting of Dendranthema grandiflorum 'Iwanohakusen' (국화 '백선'의 플러그 삽목시 삽수조건이 발근 및 절화생육에 미치는 영향)

  • Yoo, Yong Kweon;Roh, Yong Seung
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.256-261
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    • 2009
  • This study was carried out to examine the effects of cutting condition on the rooting and their subsequent growth of cut flower in plug cutting of summer-flowering chrysanthemum 'Iwanohakusen'. The more leaves were attached to the cutting, the higher shoot growth and rooting were. Shoot growth of cuttings was not affected by cutting length, but rooting was better in over 5 cm long cutting. Shoot growth was enhanced by increasing thickness of cutting, and 5.5 mm thick cutting was favored in rooting. When the many leaves were attached on the cutting, the length and weight of cut flower increased. Over 7 cm long cutting showed higher values in growth of cut flower and ray flower number. Cutting of 5.5 mm diameter showed more favorable growth of cut flower than the other treatments. Therefore, it is concluded that rooting of cutting and consecutive growth of cut flower was improved when 7 cm long and 5.5 mm diameter cutting with 4 leaves was used in plug cutting of chrysanthemum 'Iwanohakusen'.