• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.13
• /
• pp.5117-5121
• /
• 2014

Endocrine-disrupting chemicals (EDCs) have been reported to interfere with estrogen signaling. Exposure to these chemicals decreases the immune response and causes a wide range of diseases in animals and humans. Recently, many studies showed that licorice (Glycyrrhiza glabra) root extract (LRE) commonly called "gamcho" in Korea exhibits antioxidative, chemoprotective, and detoxifying properties. This study aimed to investigate the mechanism of action of LRE and to determine if and how LRE can alleviate the toxicity of EDCs. LRE was prepared by vacuum evaporation and freeze-drying after homogenization of licorice root powder that was soaked in 80% ethanol for 72 h. We used 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as an EDC, which is known to induce tumors or cancers; MCF-7 breast cancer cells were used as a tumorigenic model. These were treated with TCDD and various concentrations of LRE (0, 50, 100, 200, $400{\mu}g/mL$) for 24, 48, and 72 h. As a result, TCDD stimulated MCF-7 cell proliferation, but LRE significantly inhibited TCDD-induced MCF-7 cell proliferation in a dose- and time-dependent manner. Expression of TCDD toxicity-related genes, i.e., aryl hydrocarbon receptor (AhR), AhR nuclear translocator, and cytochrome P450 1A1, were subsequently down-regulated by LRE in a dose-dependent manner. Analysis of cell cycle distribution after treatment of MCF-7 cells with TCDD and various concentrations of LRE showed that LRE inhibited the proliferation of MCF-7 cells via G2/M phase arrest. Reverse transcription-polymerase chain reaction and Western blot analyses also revealed that LRE dose-dependently increased the expression of the tumor suppressor genes p53 and p27 and down-regulated the expression of cell cycle-related genes. These data suggest that LRE can mitigate the tumorigenic effects of TCDD in breast cancer cells by suppression of AhR expression and cell cycle arrest. Thus, LRE can be used as a potential toxicity-alleviating agent against EDC-mediated disease.

• Korean Journal of Weed Science
• /
• v.9 no.3
• /
• pp.245-249
• /
• 1989

The effects of varying concentrations and durations of butachlor [N-(bytoxymety 1,)-2-chlor -2, 6-diethy lacetanilide treatment on oat(Avena sativa L.) root cell division and protein synthesis were studied. The highest concentration ($1{\times}10^{-3}M$) of butachlor caused the significant inhibition of cell division after 18hrs treatment. After 18hrs treatment, 59% and 82% inhibition of cell division occurred at $1{\times}10^{-4}M$ and $1{\times}10^{-3}M$, respectively, while 9% inhibition of cell division did at $1{\times}10^{-6}M$ concentration at the same exposure period. To investigate protein synthesis, the oats were treated for 18 and 24hrs with concentrations ranging from $1{\times}10^{-6}M$ to $1{\times}10^{-3}M$ butachlor. After 18hrs, butachlor treatment of oat with $1{\times}10^{-4}M$ inhibitited 23% protein synthesis, and butachlor treatment with $1{\times}10^{-4}M$ caused 34% inhibition after 24hrs. With SDS-PAGE of proteins extracted from oat root tips, butachlor usually inhibited the 16, 18, 30, 43 and 43.5 kD polypeptide, and proteins of root tips are made up of subunits below 100 kD polypeptide.

• Restorative Dentistry and Endodontics
• /
• v.35 no.5
• /
• pp.395-400
• /
• 2010

The development of adhesive dentistry has allowed that the crown fragment reattachment can be another option in the treatment of crown fracture. However, additional crown lengthening procedure or extrusion of the tooth may be necessary in the treatment of crown root fracture because subgingival fracture line in close proximity to the alveolar bone leads to challenges for restorative procedure and the violation of the biologic width. This case report presents a modified crown fragment reattachment technique of crown root fracture with pulp exposure, which was done without additional crown lengthening procedures. After the endodontic treatment, the patient was treated using a post insertion and the fragment reattachment technique, which made it possible to preserve the space for the biologic width and maintain a dry surgical field for adequate adhesion through the modification of the fractured coronal fragment. Since a coronal fracture was occurred and reattached afterward, it was observed that the coronal fragment was well maintained without the additional loss of periodontal attachment through 2-year follow up.

• Korean Journal of Weed Science
• /
• v.8 no.1
• /
• pp.37-44
• /
• 1988

A serial study on differential response in absorption and translocation of $^{14}C$-oxyfluorfen was conducted by use of a group of selected rice cultivars as the tolerant or the susceptible to oxyfluorfen. Trial 1. Differential response in absorption and translocation of selected cultivar group. The susceptible cultivar group has reached as higher rate as 209%, 193%, 344%, 204% and 152% of the tolerant cultivar group in root absorption rate per unit dry weight, lower-shoot absorption rate, higher shoot absorption rate, whole amount of absorption, and the rate of translocation from bottom to shoot, respectively. Trial 2. Differential Response in Absorbtion and translocation of selected cultivar as affected by exposed portion and time. ${\bullet}$ Regardless of cultivar, the rate of root absorption has effectively realized but the translocation was significantly limited. ${\bullet}$ Lower portion of shoot has also achieved a higher amount of absorption but the translocation was very limited. ${\bullet}$ By relaying the exposure time. the amount of root absorption was ted increasing, and persistent absorption was rather effective in the susceptible (cv. Mushakdanti) cultivar than the tolerant (cv. Chokoto). ${\bullet}$ Translocation of oxyfluorfen from shoot to root was tended to easily attaining in both cultivars.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.1
• /
• pp.36-43
• /
• 2016

The aim of this study was to evaluate the effect of blood contamination on the push-out bond strength and surface morphology of tricalcium silicate materials; Biodentine$^{(R)}$, Theracal$^{(R)}$ and mineral trioxide aggregate. The standardized lumens of root slices prepared from extracted single-root human teeth were filled with Biodentine$^{(R)}$, Theracal$^{(R)}$ and mineral trioxide aggregate by manufacturer's instruction. The specimens were randomly divided into 2 groups (n = 20) for each material and then incubated for 4 days at $37^{\circ}C$; control group (phosphate buffered saline solution) and experimental group (fetal bovine serum). The push-out bond strengths were then measured by a universal testing machine and the surface morphology of each experimental group was analyzed by scanning electron microscope. Biodentine$^{(R)}$ and Theracal$^{(R)}$ showed higher push-out bond strength compared with mineral trioxide aggregate after exposure to fetal bovine serum. A substantial change in the surface morphology of each material was observed after exposure to fetal bovine serum. In conclusion, the push-out bond strengths of Biodentine$^{(R)}$ and Theracal$^{(R)}$ were higher than mineral trioxide aggregate when exposed to blood contamination. Therefore, it is supposed that the use of Biodentine$^{(R)}$ and Theracal$^{(R)}$ is appropriate in the presence of blood.

• Journal of Korean Society of Forest Science
• /
• v.86 no.1
• /
• pp.80-86
• /
• 1997

The objective of this study was to investigate how resistant poplar hybrid makes compensation to ozone stress. Growth, net assimilation rate and initial Rubisco activity were investigated. This study elucidates the physiological mechanisms associated with ozone sensitivity and resistance in 3 selected $F_2$ hybrids, a family originating from a cross between Populus trichocarpa${\times}$P. deltoides. Open-top chambers were used. Ozone concentrations varied from 90 to 115 ppb for 126 days, 6 to 9 hours in a day. This study tested the hypothesis that resistant poplar hybrid maintains the biomass production to ozone exposure via increased net assimilation rate and Rubisco activity. Growth, biomass, net assimilation rate and initial Rubisco activity were generally reduced by ozone treatment. In the tree parts, root under ozone stress was the most sensitive part. Reduced allocation of photosynthates to root growth might be due to increased respiratory demands for maintenance and repair of aboveground tissue damaged by ozone stress. Maintenance or increases remaining leaves in photosynthetic rates and Rubisco activity in resistant clone in response to ozone treatment were the results of biological compensation to ozone stress.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.17 no.3
• /
• pp.21-34
• /
• 1990

Trail and campground deteriorations and their environmental changes of soil and vegetation were studied in Tonghwa -Sa district of Mt. Palgong Natural Park through 1988 into 1989. The results are summarized as follows : 1. The bare width of trail as one of the trail condition factor was significantly greater on the more heavily used trail. Deterioration types of trail which had higher frequency for a total of 45 observations were rock exposure(42%), trail deepening(40%) and root exposure(18%) in the high order. And also sound type on which the trail was not deteriorated at all amounted to 33% of all observations. 2. Ecological changes of soil and vegatation of trailsides were not found at a uniform tendency except that soil hardness was slightly decreased from trail edge to the forest, but they could be grouped by the type of user\`s behavior and site conditions. Use impacts on the natural environment of trailside in Mt. Palgong are still far from the severe harmulness. 3. Worse damages to compground condition were appeared in Bawi-gol than Susu-gol campsite. Types of mechanically damaged trees were tree with exposed root(63% ), scared tree(43% ), mutilated tree(30 % ) and felled tree in highly frequent order. And diameter increments of trees in campsites were oppressed by the campers. 4. Tree damage types and their frequencies could be basic as a means of which grasp the bounds of user's impacts. User's disturbance on campsite extended to the distance of 70∼90m from the core of campsite at Pawi-gol and 20∼30m at Susu-gol respectively. As the tolerant trees to use impact, Lespedeza maximowiczii, Rhus trichocarpa, Acer palmatum, Rubus crataegifolius and Celastrus orbiculatus were considered.

• Journal of Food Hygiene and Safety
• /
• v.34 no.4
• /
• pp.325-333
• /
• 2019

The present study was carried out to identify the distribution characteristics of heavy metals in ginseng and wood-cultivated ginseng reduction of dietary exposure. Samples of ginseng and wood-cultivated ginseng were collected from 14 and 5 regions across Korea, respectively. Lead (Pb), cadmium (Cd) and arsenic (Ar) were detected by ICP-MS after microwave digestion, whereas aluminum (Al) was determined using ICP. Cultivated ginseng peels were 16.2% of whole root, while the peels and fine roots amounted to 21.8% and 16.8% of whole wood-cultivated ginseng, respectively. Taking into account the weight and concentration of the heavy metals by root part, their distribution ratios were calculated and compared. The cultivated ginseng peels contained 40.3% Pb, 25.9% Cd, 47.6% As, and 89.9% Al. Meanwhile, heavy metals consisting of 27.2% Pb, 28.2% Cd, 48.3% As, and 56.8% Al were distributed in the peels of the wood-cultivated ginseng. There was no significant difference between the peels and fine roots of the wood-cultivated ginseng with respect to the distribution proportion of heavy metals, except for Al. These results indicate that the level of dietary exposure to heavy metals could be reduced by peeling ginseng and wood-cultivated ginseng prior to consumption.

• Korean Journal of Environment and Ecology
• /
• v.9 no.2
• /
• pp.211-220
• /
• 1996

5 major trails of Odaesan National Park were selected to investigate the use impacts on environmental deterioration of trail accreding to the different amunt of use. The entire width, bare width, maximum depth and slope of trail as the trail comdition surveyed at the total of 120 points were significantly greater on the more heavily-used trail. Major deterioration types of trail were root-exposure, rock-exposure, deepening and divergence in order of frquency and trail conditions of deteriorated points were significantly different from those of non-deteriorated points. The damaged area more severe than Class 4 on trail reached about 379m$^{2}$/km in total, and especcially about 1,196m$^{2}$/km in the section between Chodmyolbogung and Pirobong of Sangwonsa trail. The dominant trees of trail edge vegetation could be Quercus mongolica in upper layer, but in lower layer Rhododecdron schilipenbachii for slope and Actinidia arguta for ridge. Coverage and number of individuals of lower vegetation in trail edge were the lowest on the Sangwonsa trail of the largest use amount, and each trail was dissimilar in species composition of lower layer of edge vegetation.

• Korean Journal of Agricultural Science
• /
• v.31 no.2
• /
• pp.87-96
• /
• 2004

This study concentrates on the trail of the CNU(Chungnam National University) forest. The amount of use and the pattern of damage are considered, as well as the extent of environmental damage. Finally, some managerial strategies are proposed in connection with sustainable maintenance. The trail of the university forest is composed of three sections; one is the neighborhood park and the Institute of Information Technology/Communications, the second is the Institute of Information Technology/Communications and the Dormitory, and the third section is the Dormitory and the Foreign prof's Apt. The total length of the trail is 2.6km. The daily amount of use is 1,269 visitors per day. The amount of using increased three times the amount of that in 1998. This study shows the pattern of damage of the trail in the order of root-exposure, widening, rock-exposure, deepening and the divergence in the frequency of occurrence of all these factors. Environmental damage is also graded thus: grade 3 is 27.4m, grade 4 is 0.8m, grade 5 is 1.5m, and grade 6 is 0.2m. Accordingly, some managerial strategies were proposed in connection with the sustainable trail maintenance.