• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.515-521
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• 2018

This was carried out to develop a chromosome-doubled (12x) persimmon that will be used as a crossing parent to select seedless persimmon cultivars with the change of the consumption trend recently. To obtain a chromosome-doubled (12x) persimmon, colchicine was applied at the meristem of seedlings in vitro derived from cross among hexaploid persimmon (Diopyros kaki Thunb.). These were treated with 0.03%, 0.05% and 0.1% colchicine respectively for doubling chromosome, and it was most effective at the concentration of 0.05% colchicine. After colchicine treatment, we conducted tests to elucidate conditions for inducing shoot and root development. As the result, the shoots grew best when cultivated at 1/2MS media plus 10 and $30{\mu}M$ zeatin respectively, and the roots grew best when cultivated at 1/2MS media after dipping for 5 seconds at 10 mM NAA+5% DMSO. We also compared seedlings that have chromosome (6x) do not doubled and crossing parents (6x) and chromosome-doubled seedlings (12x). As the result, these chromosome-doubled seedlings (12x) showed lower stomatal density and larger stomatal size.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.225-225
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• 2017

This study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Acorus graminens S. And it accomplished one's purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm's income. Method of fertilization application were conducted under five condition compose to standard application(N-P-K-Compost: fermented mixed oil cake fertilizer applied at $4-2-6-300kg.10a^{-1}$, twofold application(N-P-K-Compost applied at $8-4-12-600kg.10a^{-1}$) cultivation. Compost (fermented mixed oil cake fertilizer) and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application and twofold application(N-K) applied at 100%, 50%, 40% of basal fertilizer in April 14, 50% and 40% of top dressing were three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Transplanting with Naju varieties in May 12 by growing pot seedling for 40 days in 2015. Planting density were spaced 30 cm apart in rows 30 cm apart with black vinyl mulching on open cultivation. Plot design randomized block 3 repetition. Therefore, growth characteristics by 50% and 40% of top dressing were three times application (N-K) compared to standard application and twofold application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of diameter and main root of subterranean part growth increment rising highest. Dried roots yields treated standard fertilizer 100% application($250kg.10a^{-1}$) compared to 100% from twofold application (N-K) applied at 50% basal fertilizer, 50% and 40% of top dressing were three times application increased by 16%, 20%. respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.2
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• pp.140-145
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• 1997

The change of physico-chemical properties of paddy soil was monitored under different tillage. This study was conducted on Jeonbug silty cly loam, in Honam Agricultural Experiment Station, for four years starting from 1992. Different tillage involved (1)Continued no tillage, (2)Rotavation only with tractor every year, (3)Spring plowing with power tiller plus rotavation with tractor every year. The result of this study can be summarized as: Non tillage, when continued for some years, tended to increase 1)the hardness of soil, 2)the root density in the top soil, 3)the occurrence of annual and perennial weeds, and tended to lower the yield of rice compared to normal tillage(tilling with ow tiller in the spring plus rotavation by tractor before planting.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.77-88
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• 1992

Effective strains of cowpea bradyrhizobia JB7 $nal^rspe^r$ and CB756 $str^rrif^r$, antibiotic-resistant variants of JB7 and CB756, respectively, were used to examine changes of rhizosphere populations and nodule occupancy. Populations of each strain increased gradually in the rhizosphere, reaching a maximum of about $10^8$ cells per root system. Nodule number increased as the density of inoculum increased from $10^2$ cells to $10^8$ cells per seed. Inoculation with liquid suspension resulted in the formation of more nodules than the peat slurry or granule inoculation. When JB7 $nal^rspe^r$ and CB756 $str^rrif^r$ were introduced in equal numbers in inoculum mixtures the former consistantly occupied the majority of nodules with all three groundnut cultivars used. There was no difference in yield between nitrogen treatments and inocultation treatments.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.2
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• pp.127-132
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• 1992

This experiment was conducted to find out the useful data for upland crop cultivation in the newly reclaimed tidal land. Poseung silty clay loam soil was selected, and cotton(Mogpo VII) and tall fescue were cultivated under different drainage systems and soil ameliorator applications. Soil hardness and bulk density were decreased by subsurface drainage and plastic film installed at 40cm depth of the soil. Red earth application was also effective to loosen the soil, but zeolite and gypsum made the subsoil compact. Water content of the soil was high in surface drain than that of subsurface drain or plastic film curtain plot during dry season. The water content was in order of plastic film curtain, surface drain and subsurface drain. Electrical conductivity(EC) was decreased to lower than 0.4 Simens $meter^{-1}$ ($SM^{-1}$) in the subsurface drain during rainy season, and the EC of subsurface drain was maintained a quater to an half of surface drain. The yield of cotton and tall fescue were high in order of subsurface drain, plastic film curtain and surface drain plot. The yields of cotton were increased to 36-73 % by ameliorator application, and the red earth application was more effective for tall fescue growth compare to gypsum and zeolite.

• Journal of Environmental Science International
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• v.18 no.4
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• pp.463-471
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• 2009

A study on vegetation in the Mangyeong River and Dongjin River basins and the surrounding regions of the Saemangeum Reclaimed Land was conducted in a series of efforts to determine the expected ecological changes in the salt marshes, to restore their vegetation, to explore the restoring force of halophyte, to examine the community mechanism and, ultimately, to rehabilitate marshy land vegetation along the lakeside, coastal dune and salt marshes of the Saemangeum Project Area. The findings of the study may be summed up as follows: Five species such as Suaeda japonica, Salicornia herbacea, Atriplex gmelini, Aster tripolium and Suaeda asparagoides that are mostly distributed in the estuary of the Saemangeum Reclaimed Land were analyzed to examine the mechanism of halophyte to maintain their community. To find out the strategies of plants for survival and the cause of forming community structure, a research was made as for appearance ratio of biomass, root lengths and germination. With regard to biomasses of halophyte, the biomass of Suaeda japonica increases rapidly, while Salicornia herbacea adopts a strategy of unstable growth pattern by which the biomass increases slowly in parallel with its slow speed of growth since initial appearance of young sapling. In contrast, Suaeda asparagoides, Atriplex gmelini and Aster tripolium choose to adapt themselves to environment promptly by being transformed into life form of annual or biennial plant, a pattern that is presumed to be favorable and stable for survival in the later stage of growth. In short, there is a sharp distinction among the 5 species: i.e. Suaeda japonica that begins to grow fast in the length of surface and underground section but slows down from the mid-stage on; Salicornia herbacea that grows slowly in the beginning step but starts to step up from the middle onward; Atriplex gmelini and Aster tripolium growing slowly in the initial stage but fast later on; and Suaeda asparagoides that turns from the constant growth in the beginning to rapid growth in the later period. The outcomes of the analysis into status of growth and influencing factors of Suaeda japonica in the sowing field that is most widely prevalent in the Saemangeum Reclaimed Land as a sort of ecological pioneer in the salt marshes showed that the average size of grass lands, density and number of individuals increase in the natural sowing field as well as in the plowing field regardless of their physical as well as physico-chemical features of the soil as the season progresses from June to October of a year.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.169-178
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• 1999

The natural regeneration pattern was evaluated for 11 useful hardwood species in the natural deciduous forest where three different silvicultural practices (two-storied system, shelterwood system, and selection system) were applied and passed by three growing seasons. The study forest was presently dominated by Quercus mongolica in the upper canopy. However, since the analysis indicated that the number of regenerated seedlings of Acer mono and Fraxinus rhynchophylla was fairly abundant enough, these two species were anticipated to have the possibility to be among dominant species along with Quercus mongolica. The results of seedling occurrence after regenerating cutting showed that the number of seedlings was estimated for 3,145/ha(10 times of overstory) in the two-storied system, 6,885/ha(9 times of overstory) in the shelterwood system, and 2,275/ha(4 times of overstory) in the selection system. The estimation was varied by species, and the number of stump sprout was more abundant. The proportion of seedlings was high in 60~80% of overstory density and 60~70% of understory coverage, and high in the site of less than $25^{\circ}$ of slope and from southwest to northwest of aspect. These results were still varied by species. Stump diameter and height had little influence on the occurrence of sprout.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.3
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• pp.254-261
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• 2012

To reduce the injury by continuous cropping of sweet potato (Ipomoea batatas (L.) Lam.), the farmer's plant and virus-free plant were cultivated with the density of $70{\times}25cm$ (June 10, 2011) in continuous cropping soil (CCS) and subsoiling reversion soil (SRS). Fertilizer was applied at the rates of 55-63-156 $kg\;ha^{-1}$ ($N-P_2O_5-K_2O$) and 10 $ton\;ha^{-1}$ of cattle manure in CCS, and it was applied the 50% increased cattle manure compost and nitrogen in DRS. Symptoms of viral infection were revealed in the farmer's plant at 30 days after planting, but there were no symptoms in virus-free plant. The yield of virus-free plant was more increased 15% and 10.5% than that of farmer's plant in DRS and CCS, respectively. The yield of sweetpotato in SRS was more increased 8.8% and 3.2% in farmer's plant and virus-free plant compared to CCS, respectively. In DRS, the rate of marketable tuber of virus-free plant was increased by 80% compared to the farmer's plant (60.1%). The virus-free plant was produced the tuber with more brilliant peel color and well-formed shape compared to the farmer's plant. The increased yield of virus-free plant and in SRS soil condition showed a positive relationship (p=0.05) with the number of leaf per plant at 30 days and the number of branch per plant at 120 days after planting. The results showed that the early growth after planting was very important for the development of storage root. Therefore, the deep-subsoil reversion and cultivation of virus-free plant could be reduced the injury by continuous cropping of sweet potato, and increased farm income.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.619-630
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• 2009

To investigate the hair growth activity of fractions and extract of Arisaematis Rhizoma in the hair removed skin of normal and spontaneous alopecia areata model in C57B/6N mice. These experiments were performed with the macroscopic, microscopic, immunohistochemical(VEGF, c-kit, PKC-${\alpha}$, TGF and FGF) and RT-PCR(TGF-${\beta}$, IGF, prolactin and placenta lactogen) methods. The results were as follows: Macroscopic observation after topical application of vehicle, 50% EtOH as control and extract of Arisaematis Rhizoma to the hair removed skin of C57BL/6N mice on the 9th, 11th and 15th day. Extensive hair growth activity was observed in treated group with extract of Arisaematis Rhizoma on the 9th, 11th and 15th day. In Arisaematis Rhizoma extracts treated group, hair follicles of middle stage of anagen was observed and it were grown down to subcutaneous tissue of skin in all the normal mice on 15th day. But in control group, most of hair follicles of telogen phase was observed in skin. The treatment of extract of Arisaematis Rhizoma increased expression of IGF(145%) and placenta lactogen(108%) in the skin of normal C57BL/6N mice on the 11th day compared to control group(100%). But expression of TGF-${\beta}$(90%) and prolactin(91%) decreased in the skin of normal C57B/6N mice on the 11th day compared to control group(100%). After application of fractions(chloroform, ethyl acetate and water fractions) of Arisaematis Rhizoma extract for 9th day, hair growth effect was observed in whole skin area in 50% of normal mice. But in control group, hair growth effect was not observed in whole skin area of normal mice. Immunoreactive density of VEGF, c-kit, PKC-${\alpha$ and FGF in skin of fractions of Arisaematis Rhizoma extracts was strongly stained in epidermis, bulge, secondary hair germ cells, cutaneous trunci m., subcutaneous tissue, root sheath compare to control group on the 9th day. In spontaneous alopecia areata model, The hair growth activity of Arisaematis Rhizoma extrat treated group(75%) was observed to be strong compared to control group(O%) on 7th day. These experiments suggest that fractions and extracts of Arisaematis Rhizoma may stimulate the topical hair growth activity. Thus it can be useful for treatment of alopecia areata.

• Korean Journal of Pharmacognosy
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• v.46 no.1
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• pp.78-83
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• 2015

This study was conducted to verify the potential of Allium hookeri to control glucose metabolism in a diabetes model. We fed the experimental diets (AL, AR, Dex) supplemented with the powder of leaf, root, or dextrin as a positive control, respectively at 3% of diet to the diabetic mice (C57BLKS/J, db/db) for 8 weeks. Control mice were fed with the diet supplemented with cornstarch (Cont) at 3% level of diet. At 8th week of feeding the diets, we measured body weight, blood glucose, HbA1c, and plasma insulin levels and conducted an oral glucose tolerance test (OGTT) and staining insulin immunoreactive cells in islets of pancreas. AL group treated with the leaf of A. hookeri showed significantly lower blood glucose and HbA1c levels, higher plasma insulin levels, and increased density of insulin immunoreactive cells compared with the Cont group. During the OGTT, AL group showed lower blood glucose levels than the Cont group for 120 min. Based on these results, leaf of A. hookeri is considered to be effective in improving glucose tolerance by partially affecting insulin secretion and it may be used to prevent and treat diabetic disease.