Browse > Article

Experimental Studies on the Hair Growth Activity of Fractions and Extract of Arisaematis Rhizoma in C57B/6N Mice  

Kwon, Kyung-Suk (Department of sasang Constitutional Medicine, College of Oriental Medicine, Woosuk University)
Lee, Moon-Won (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jeong, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jeong, Han-So (Department of Pathology, College of Oriental Medicine, Woosuk University)
Song, Beom-Yong (Department of Acupuncture & Moxibution, College of Oriental Medicine, Woosuk University)
Song, Jeong-Mo (Department of sasang Constitutional Medicine, College of Oriental Medicine, Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.3, 2009 , pp. 619-630 More about this Journal
Abstract
To investigate the hair growth activity of fractions and extract of Arisaematis Rhizoma in the hair removed skin of normal and spontaneous alopecia areata model in C57B/6N mice. These experiments were performed with the macroscopic, microscopic, immunohistochemical(VEGF, c-kit, PKC-${\alpha}$, TGF and FGF) and RT-PCR(TGF-${\beta}$, IGF, prolactin and placenta lactogen) methods. The results were as follows: Macroscopic observation after topical application of vehicle, 50% EtOH as control and extract of Arisaematis Rhizoma to the hair removed skin of C57BL/6N mice on the 9th, 11th and 15th day. Extensive hair growth activity was observed in treated group with extract of Arisaematis Rhizoma on the 9th, 11th and 15th day. In Arisaematis Rhizoma extracts treated group, hair follicles of middle stage of anagen was observed and it were grown down to subcutaneous tissue of skin in all the normal mice on 15th day. But in control group, most of hair follicles of telogen phase was observed in skin. The treatment of extract of Arisaematis Rhizoma increased expression of IGF(145%) and placenta lactogen(108%) in the skin of normal C57BL/6N mice on the 11th day compared to control group(100%). But expression of TGF-${\beta}$(90%) and prolactin(91%) decreased in the skin of normal C57B/6N mice on the 11th day compared to control group(100%). After application of fractions(chloroform, ethyl acetate and water fractions) of Arisaematis Rhizoma extract for 9th day, hair growth effect was observed in whole skin area in 50% of normal mice. But in control group, hair growth effect was not observed in whole skin area of normal mice. Immunoreactive density of VEGF, c-kit, PKC-${\alpha$ and FGF in skin of fractions of Arisaematis Rhizoma extracts was strongly stained in epidermis, bulge, secondary hair germ cells, cutaneous trunci m., subcutaneous tissue, root sheath compare to control group on the 9th day. In spontaneous alopecia areata model, The hair growth activity of Arisaematis Rhizoma extrat treated group(75%) was observed to be strong compared to control group(O%) on 7th day. These experiments suggest that fractions and extracts of Arisaematis Rhizoma may stimulate the topical hair growth activity. Thus it can be useful for treatment of alopecia areata.
Keywords
Arisaematis Rhizoma; hair growth; immunohistochemistry; VEGF; c-kit; PKC-${\alpha}$; TGF; FGF; RT-PCR; TGF-${\beta}$; prolactin; IGF; placenta lactogen;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 최근희 외 11인. 모발과학. 서울, 수문사, pp 8-14, 2001
2 Selmanowilz, V., Victor, S., Warburton, D., Orentreich, N. Fingerprints arches in alopecia areata. Arch Dermatol 110: 570-571, 1974   DOI   ScienceOn
3 Toback, C., Ajkunar, S. The emotional disturbances underlying alopecia areata, alopecia totalis and trichotillomania. Child Psychiatry Hum Dev 10: 114-117, 1979   DOI   ScienceOn
4 Li, L.F., Guo, J., Gao, Z.F. Overexpression of skin protein kinase c-alpha in anagen hair follicles during induced growth of mouse hair. Clin Exp Dermatol 28: 429-433, 2003   DOI   ScienceOn
5 임사비나, 최규동, 김수경. 탈모에 대한 동서의학적 고찰. 경산대학교 제한동의학술원 논문집, 4(1):699-710, 1994
6 정석영, 정일국, 김대근, 조한영, 정한솔, 이창현. C57BL/6N 생쥐에서 골쇄보 추출물을 이용한 발모효과에 대한 실험적 연구. 동의생리병리학회지 21(2):453-461, 2007
7 姜义娜. 斑秃药物治疗的新进展. 中国皮肤性病学杂志, 17(3): 203-204, 2003
8 Birch, D.E., Kolmodin, L., Laird, W.J., McKinney, N., Wong, J., Young, K.K.Y., Zangenberg, G.A., Zoccoli, M.A. Simplified Hot-Start PCR. Nature 381: 445-446, 1996   DOI   ScienceOn
9 Hsu, S.M., Raine, L., Fanger, H. Use of avidin-biotin-peroxidase complex(ABC) in immunoperoxidase techniques : A comparison between ABC and unlabeled antibody(PAP) procedures. J Histochem Cytochem 29: 577-580, 1981   DOI
10 이영주, 이주영. 남성의 안드로겐성 탈모자의 임상적 진단과 관리. 한국미용학회지 13(2):799-810, 2007
11 Peters, E.M., Maurer, M., Botchkarev, V.A., Jensen, K., Welkr, P., Scott, G.A., Paus, P. Kit is expressed by epithelial cells in vivo. J Invest Dermatol 121: 976-984, 2003   DOI   ScienceOn
12 葛洪. 肘後備急方. 北京, 人民衛生出版社, p 121, 1982
13 탁민진, 정일국, 김대근, 정한솔, 이창현. 측백엽 추출물의 발모효과에 관한 실험적 연구. 동의생리병리학회지 21(1):136-144, 2007   과학기술학회마을
14 Gartner, L.P., Hiatt, J.L. Color textbook of histology(3rd), Elsevier, pp 339-340, 2007
15 한의과대학본초학편집위원회. 본초학, 서울, 영림사, pp 486- 487, 2007
16 陳存仁. 漢方醫藥大事典, 圖書出版 松嶽, pp 296-297, 1998
17 Detmar, M. Molecular regulation of angiogenesis in the skin. J Invest Dermatol 106: 207-208, 1996   DOI   ScienceOn
18 裵正学, 侯瑞云, 传秀琴. 辨证治疗斑秃和早秃73例初步报告. 中医杂誌 28: 43-44, 1987
19 이문원. 자발적 원형탈모 생쥐(C57BL/6N)와 정상 생쥐에서 반모와 골쇄보 추출 혼합물의 발모에 대한 실험적 연구, 우석대학교대학원(박사), 2008
20 吴自强, 尤菊松. 天南星在外治法中的应用. 中医外治杂誌 4期: 36-37, 1997
21 Cotsarelis, G., Sun, T.T., Lavker, R.M. Label-retaining cells reside in the bulge area of pilosebaceous unit : implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 61: 1329-1337, 1990   DOI   ScienceOn
22 朱仁康. 脱髮证治. 中医杂誌 2(12): 46, 1986
23 杨中林, 韦英杰, 杜慧, 宣圆圆. 东北南星不同炮制品的毒性及刺激性硏究. 中成药, 23(2):101-103, 2001
24 Tsuji, Y., Denda, S., Soma, T., Raftery, L., Momoi, T., Hibino, T. A potential suppressor of TGF-beta delays catagen progression in hair follicles. J Invest Dermatol Symp Proc 8(1):65-68, 2003   DOI   ScienceOn
25 Argyris, T.S. The effects of wounds on adjacent growing or resting hair follicles in mice. Arch Dermatol Symp 61: 31-36, 1956
26 Argyris, T. Kinetics of epidermal production during epidermal regeneration following abrasion in mice. Am J Pathol 83: 329-340, 1956
27 홍석훈, 노석선. 모발탈락의 병인 및 치료법에 대한 문헌적 고찰. 외관과학회지 9(1):43-65, 1996
28 Muller, H.K., Rook, A.J., Kubba, R. Immunohistology and autoantibody studies in alopecia areata, Br J Dermatol 102: 609-610, 1980   DOI   ScienceOn
29 赵成林. 中药引赤发泡药简介. 江苏中医, 17(5):37-38, 1996
30 娄国菁, 滕颍. 发泡療法及其临床应用. 时珍国医国药, 12(12): 1126-1128, 2001
31 韦英杰, 杨中林. 天南星硏究进展. 时珍国医国药, 12(3):264- 267, 2001
32 李林. 实用中医皮肤病学. 北京, 中医古籍出版社, pp 60-61, 1998
33 Harada, N., Okajima, K., Narimatsu, N., Kurihara, H., Nakagata, N. Effect of topical application of raspberry ketone on dermal production of insulin-like growth factor-1 in mice and on hair growth and skin elasticity in humans. Growth Horm IGF Res. 18(4):335-344, 2008   DOI   ScienceOn
34 Stenn, K.S., Paus, R. Controls of hair follicle. Physiological Reviews 81: 449-494, 2001   DOI
35 巢元方. 諸病源候論校釋. 北京, 人民衛生出版社, pp 761-767, 1983
36 楼之岑, 秦波. 常用中药材品种整理和质量硏究, 北京大学医学出版社, pp 975-1022, 1995
37 Bergfeld, W. Diffuse hair loss in women. Cutis 22: 190-195, 1978   PUBMED
38 Kwack, M.H., Sung, Y.K., Chung, E.J., Im, S.U., Ahn, J.S., Kim, M.K., Kim, J.C. Dihydrotestosterone-inducible dickkopf 1 from balding dermal papilla cells causes apoptosis in follicular keratinocytes. J Invest Dermatol 128(2):262-269, 2008   DOI
39 Foitzik, K., Krause, K., Nixon, A.J., Ford, C.A., Ohnemus, U., Pearson, A.J., Paus, R. Prolactin and its receptor are expressed in murine hair follicle epithelium, show hair cycle-dependent expression and induce catagen. Am J Pathol 62: 1611-1621, 2003
40 肖培根. 新编中药誌, 北京, 化学工业出版社, pp 165-175, 2002
41 Tausk, F., Christian, E., Johansson, O., Milgram, S. Neurobiology of the skin. In Fitzpatrick, T.B., Eisen, A.Z., Wolff, K., Freedberg, I.M., Austen, K.F.(eds) Dermatology in General Medicine(4th ed), New York, McGraw-Hill, pp c, 1993
42 Otomo, S. Hair growth effect of minoxidil. Nippon Yakurigaku Zasshi 119(3):167-174, 2002   DOI   PUBMED   ScienceOn
43 汪蕾, 张继振. 天南星属植物硏究进展. 延边大学学报, 30(1):66- 72, 2004
44 吴皓, 鐘凌云. 天南星科有毒中药刺激性作用比较硏究. 中国中药杂誌, 33(4):380-384, 2008
45 Friedman, P.S. Alopecia areata and auto-immunity. Br J Dermatol 105: 153-157, 1981   DOI   ScienceOn
46 許浚. 東醫寶鑑, 서울, 법인문화사, pp 793-797, 1999
47 Silver, A.F., Chase, H.B., Arsenault, C.T. Early anagen initiated by plucking compared with early spontaneous anagen. Adv Biol Skin 9: 265-286, 1969
48 김용익, 이문원, 권진, 송창호, 이창현. 자발적으로 탈모된 생쥐 모델을 이용한 탈모관련 인자에 관한 연구. 대한해부학회지 38(2):133-143, 2005
49 조한영. C57BL/6N 생쥐에서 반모추출물과 분획물을 이용한 발모효과에 대한 실험적 연구. 우석대학교대학원(석사). 2007
50 楊維傑 編. 黃帝內經素問譯解. 台聯國風出版社, 일중사(영인본), pp 6-7, 94, 1991
51 Kamimura, A., Takahashi, T., Morohashi, M., Takano, Y. Procyanidin oligomers counteract TGF-beta1- and TGF-beta2-induced apoptosis in hair epithelial cells: an insight into their mechanisms. Skin Pharmacol Physiol 19(5):259-265, 2006   DOI   PUBMED   ScienceOn
52 鐘凌云, 吴皓. 天南星科植物中黏膜刺激性成分的硏究现状与分析. 中国中药杂誌, 31(18):1561-1563, 2006
53 Botchkareva, N.V., Khlgatian, M., Longley, B.J., Botchkareva, V., Gilchrest, B.A. SCF/c-kit signaling is required for cycling regeneration of the hair pigmentation unit. FASEB J 15: 645-658, 2001   DOI   ScienceOn
54 최승훈 譯. 難經入門. 서울, 법인문화사, p 93, 147, 1998
55 全國韓醫科大學鍼灸經穴學敎室. 鍼灸學(下), 집문당, 서울, pp 1059-1060, 1991
56 Ito, M., Kizawa, K., Hamada, K., Cotsarelis, G. Hair follicle stem cells in the lower bulge from the secondary germ, a biochemically distinct but functionally equivalent progenitor cell population, at the termination of catagen. Differentiation 72: 548-557, 2004   DOI   ScienceOn
57 Rashtak, S., Pittelkow, M.R. Skin involvement in systemic autoimmune diseases. Curr Dir Autoimmun 10: 344-358, 2008   DOI   PUBMED   ScienceOn
58 李元文, 张韦川. 名医临床证经验丛书 皮肤病, 北京, 人民卫生出版社, pp 572-622, 2001
59 Lynfield, Y.L., MacWilliams, P. Shaving and hair growth. J Invest Dermatol 55: 170-172, 1970   DOI   ScienceOn