• Restorative Dentistry and Endodontics
• /
• v.44 no.1
• /
• pp.8.1-8.7
• /
• 2019

Objectives: Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection. Materials and Methods: The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs). Results: The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples. Conclusions: This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis.

• Restorative Dentistry and Endodontics
• /
• v.34 no.6
• /
• pp.500-507
• /
• 2009

The purpose of this study is to compare the antibacterial effect of $Listerine^{(R)}$ on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using $Listerine^{(R)}$ as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and $Listerine^{(R)}$ were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of $Listerine^{(R)}$ and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p < 0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, $Listerine^{(R)}$ showed significantly low antibacterial effect compared with the other root canal irrigants (p < 0.05). In conclusion, the results reflect remarkably low antibacterial effect of $Listerine^{(R)}$ as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

• Restorative Dentistry and Endodontics
• /
• v.23 no.1
• /
• pp.379-390
• /
• 1998

In endodontic treatment, calcium hydroxide has been used as intracanal medicament. Although calcium hydroxide should be removed thoroughly before permanent root canal filling, no effective method for its removal has been reported. Because of irregularity of root canal walls, root curvatures and anatomic variations, it is insufficient to remove calcium hydroxide from the canal wall only by mechanical instrumentation Considering the chemical effects of irrigants on calcium hydroxide, $Ca^{++}$ dissolving effect from two calcium hydroxide products is investigated, using dis- tilled water, NaOCl, citric acid and EDTA. Vitapex$^{(R)}$ 0.1g and calcium hydroxide 0.03g were dissolved in distilled water, 5% NaOCl, 50% citric acid and 17% EDTA respectively, at 1, 3, 5, 10min. time interval. The solution was filtered using filter paper(pore size $5{\mu}m$) and $Ca^{++}$ concentration was determined by ion chromatography. The result were as follows : 1. Distilled water, NaOCl, citric acid and EDTA abstracted more $Ca^{++}$ from calcium hydroxide than Vitapex$^{(R)}$ except NaOCl 1, 5, 10 time interval. 2. EDTA and citric acid abstracted more $Ca^{++}$ from Vitapex$^{(R)}$ and calcium hydroxide than distilled water or NaOCl. The overall result support the view that water-based calcium hydroxide product is easily removed than oil-based calcium hydroxide product and EDTA, citric acid are more effective in $Ca^{++}$ elution than NaOCl or distilled water.

• Restorative Dentistry and Endodontics
• /
• v.15 no.2
• /
• pp.127-133
• /
• 1990

Electronic working length determination is based on the electrical impedence between the root canal and the periodontal ligament Different canal irrigants with different electric conductivity may effect on the accuracy of the electronic mechanism. 0.9% Saline, 2.5% NaOCl and 3% $H_2O_2$ were evaluated either in wet or in dry condition. The results were as follows. 1. The electrical resistance mearured by Impedence Meter (Model #253, portland, USA) were 1 $K{\Omega}$ in 0.9% Saline, 0.1$K{\Omega}$ in 2.5% NaOCl and 48 $K{\Omega}$ in 3% $H_2O_2$. 2. Saline measured -0.57mm from the actual canal length and had significant difference. When dried with paper points, however, it measured -0.25mm demonstrating no statistical difference. 3. 2.5% NaOCl measured -4.07mm in wet and -2.26mm in dry condition and both showed significant difference from the actual canal length. 4. 3% $H_2O_2$ measured +0.02mm in wet and -0.09mm in dry canals and both appeared to be within acceptable range for the electronic working length mechanism.

• Proceedings of the KACD Conference
• /
• 2003.11a
• /
• pp.608-608
• /
• 2003

The main objectives of root canal therapy are cleaning and shaping and then obturating the root canal system in 3 dimensions to prevent reinfection. Many instrumentation techniques and devices, supported by an irrigation system capable of removing pulp tissue remnants and dentin debris, have been proposed to shape root canals. But current regimens in chemomechanical debridement using instrumentation and irrigation with NaOCl are not predictably effective in root canal disinfection. These findings are not surprising because the root canal system is complex and contains numerous ramifications and anatomical irregularities. The microorganisms in root canals not only invade the anatomic irregularities of the root canal system but also are present in the dentinal tubules. Therefore further disinfection with an effective antimicrobial agent may be necessary and it well1mown that use of intracanal medication will lower bacterial count in infected root canals. Calcium hydroxide has a long history of use in endodontics, and more attention has been given to the use of calcium hydroxide as intracanal dressing for the treatment of infected pulp. However, when treatment is completed in one visit, no intracanal medications other than intracanal irrigants are used. Recently, a mixture of a tetracycline isomer, an acid, and a detergent(MTAD), has been introduced as a final rinse for disinfuction of the root canal system. It has been shown that MTAD is able to remove the smear layer with minimal erosive changes on the surface of dentin, and is effective against Enterococcus faecalis, a microorganism resistant to the action of other antimicrobial medications. In another study, the ability of MTAD was investigated to disinfect contaminated root canals with whole saliva and compared its efficacy to that of NaOCl Based on the results, it seems that MTAD is significantly more effective than 5.25% NaOCl in eradicating bacteria from infected root canals. In the cytotoxicity evaluation, MTAD is less cytotoxic than engenol, 3% $H20_2,\;Ca(OH)_2$ paste, 5.25% NaGCl, Peridex, and EDTA and more cytotoxic than 2.63%,1.31% and 0.66% NaOCl. Is it promising or transient?

• Restorative Dentistry and Endodontics
• /
• v.45 no.3
• /
• pp.39.1-39.11
• /
• 2020

Despite the vast literature on the effects of root canal irrigants on the dentin characteristics, the precise effects of clinically relevant irrigation sequences remain unclear. In this review, we systematically dissect the role of different sequential irrigation approaches that are used in clinical endodontics. Using a systematic search strategy, we attempt to answer the question: 'Which irrigating sequence has the most deleterious effects on dentin structure and properties?' The effect of irrigants on the dentin composition and mechanical properties have been reviewed. A wide variety of concentrations, duration and techniques have been employed to characterize the effects of chemicals on dentin properties, thus making it impossible to draw guidelines or recommendations of irrigant sequences to be followed clinically. It was apparent that all the studied irrigation sequences potentially result in some deleterious effects on dentin such as decrease in the flexural strength, microhardness, modulus of elasticity and inorganic content and organic-inorganic ratio of the dentin. However, the literature still lacks comprehensive investigations to compare the deleterious effect of different irrigation sequences, using a wide variety of qualitative and quantitative methods. Such investigations are essential to make clinical recommendations and strategize efforts to minimize chemically-induced damage to dentin characteristics.

• Restorative Dentistry and Endodontics
• /
• v.9 no.1
• /
• pp.107-114
• /
• 1983

The purpose of this study was to observe the effect of cleansing action of irrigation solutions which was 3% $H_2O_2$ and 5% NaOCl, and 15% EDTA solution on the root canal wall. After treatment with the irrigant, each sample was dehydrated, and coated with 200~250${\circ}$A of gold, and observations were made with the use of scanning electron microscope. The results were as follows: 1. In the root canal walls irrigated with 3% $H_2O_2$ and 5% NaOCl solution without instrumentation after extirpation through barbed broach, the predentin of root canal wall was found scarely affected, and the wall was shown retaining network structure and fibrous organic matters. 2. When 15% EDTA was applied as irrigants for 60, 90 and 120 seconds after instrumentation, there was no signigicant difference of the cleansing effect of the elapsed times which were 90 and 120 seconds on the root canal wall, but in the applied time which was 120 seconds, the canal wall was the cleanest. Therefore it was thought that the most suitable application time of 15% EDTA as the irigants was 120 seconds.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.4
• /
• pp.467-472
• /
• 2016

Successful root canal treatment can be obtained by the removal of microorganisms from the pulpal space of the root canal system through biomechanical technique with instruments and irrigation. Due to the difference in the dimension of the pulpal structure such as thin wall of the root, large portion of chamber, the primary teeth should be considered in a different way of approach. Traditionally, fluids have been dispensed passively into the root canals for improve the cleansing. The use of sodium hypochlorite as an irrigant in root canal treatment is widespread and common, because it meets requirements for the ideal irrigants.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.28 no.2
• /
• pp.139-145
• /
• 2012

The purpose of this study was to evaluate the accuracy of Root ZX and Sybron Mini in determining the working length using 2.5% Sodium Hypochlorite, 2% Chlorhexidine gel and saline. Donnelly's gelatin technique was used for measuring twenty extracted human teeth. Electronic working length was measured at the display 0.5 and 0.0 level of each electronic apex locator using a digital caliper to the nearest 0.01 mm. Each measurement was repeated for each different irrigants. Accuracy of Sybron Mini was not significantly different from the Root ZX in the three different irrigants, and there was no significant difference in the accuracy of electronic apex locators among the irrigants when used as recommended by manufacturer (measuring electronic working length at 0.0 level of Sybron Mini and at 0.5 level of Root ZX).

• Restorative Dentistry and Endodontics
• /
• v.23 no.2
• /
• pp.676-681
• /
• 1998

The purpose of this study is to explain theoretically the mechanism of the frequency-dependent apex locator which can detect the apex of the root in moist canals with blood or other conductible canal irrigants. The frequency-dependent apex locator is based on measuring the ratio of the two impedances of the two alternating currents with different frequencies. We analyzed the changes of the ratio between the two impedances by using differential calculus. Our analysis shows that : 1. When the file is in the moist canal, the ratio between the two impedances is almost constant. 2. As the file approaches the apex of the root, the ratio decreases sharply. By this mechanism, the frequency-dependent apex locator can detect the apex of the-root quite accurately in moist canals.