• 토지주택연구
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• 제7권2호
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• pp.87-95
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• 2016

국민기초생활보장법에 따라 2002년 시행되어 온 주거현물급여사업은 "주거급여법" 제정(2014.1.24.)으로 변화를 맞이하고 있다. 그동안 주거현물급여는 수급자 가구의 주거환경개선과 저소득층의 자활일자리 창출이라는 이중의 목표에 맞추어 13년간 사업이 시행되어 왔지만 각 지방자치단체에서의 이를 관리하고 시행하는데 따른 편차에 따라 사업의 효율성을 확보하는데 한계를 보였다. 이에 그동안 중추적인 역할을 담당했던 주거분야의 자활기업과 사업단의 매출액, 수익금의 변화와 새롭게 형성된 중앙 단위조직인 전국자활기업연합체가 향후 사업수혜자에 대한 만족도 향상과 제도변화에 따른 대응, 저소득층 일자리로서의 기능을 유지하는데 필요한 서비스 수준 향상 가능성과 사업관리체계의 변화요구에 대한 자기노력과 선도적 대응을 요청받고 있는 것 또한 사실이다. 이에 그동안의 주거현물급여사업에 대한 기존연구를 보완함과 동시에 새로운 제도 환경에 맞는 현장의 변화유인을 확인하고 그에 따른 대응을 검토하였다. 이를 위하여 첫째 전국자활기업이 저소득 대상에 대한 이해도 높은 특화된 서비스제공, 둘째 전국자활기업을 주거현물급여의 품질을 향상시키기 위한 전문화된 기관으로 전환 유도, 셋째 공급기관으로서의 수선유지에 대한 업무와 관련된 명시적인 지침의 제시를 통한 제도변화와의 조응, 마지막으로 사업수행과정프로세스에서의 일관된 기준과 절차적 표준화를 위한 혁신이 동반되어야 한다.

• Archives of Pharmacal Research
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• 제24권2호
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• pp.126-135
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• 2001

Quinacrine (QU), a phospholipase-A2 (PLA-2) inhibitor has been used clinically as a chemotherapeutic adjuvant. To understand the mechanisms leading to its chemotherapeutic effect, we have investigated QU-induced apoptotic signaling pathways in human cervical squamous carcinoma HeLa cells. In this study, we found that QU induced cytochrome c-dependent apoptotic signaling. The release of pro-apoptotic cytochrome c was QU concentration- and time-dependent, and preceded activation of caspase-9 and -3. Flow cytometric FACScan analysis using fluorescence intensities of $DiOC_6$/ demonstrated that QU-induced cytochrome c release was independent of mitochondrial permeability transition (MPT), since the concentrations of QU that induced cytochrome c release did not alter mitochondrial membrane potential (${\blacktriangle}{\Psi}_m$). Moreover, kinetic analysis of caspase activities showed that cytochrome c release led to the activation of caspase-9 and downstream death effector caspase-3, Caspase-3 inhibitor (Ac-DEVD-CHO) partially blocked QU-induced apoptosis, suggesting the importance of caspase-3 in this apoptotic signaling mechanism. Supplementation with arachidonic acid (AA) sustained caspase-3 activation induced by QU. Using inhibitors against cellular arachidonate metabolism of lipooxygenase (Nordihydroxyguaiaretic Acid, NDGA) and cyclooxygenase (5,8,11,14-Eicosatetraynoic Acid, ETYA) demonstrated that QU-induced apoptotic signaling may be dependent on its role as a PLA-2 inhibitor. Interestingly, NDCA attenuated QU-induced cytochrome c release, caspase activity as well as apoptotic cell death. The blockade of cytochrome c release by NDCA was much more effective than that attained with cyclosporin A (CsA), a MPT inhibitor. ETYA was not effective in blocking cytochrome c release, except under very high concentrations. Caspase inhibitor z-VAD blocked the release of cytochrome c suggesting that this signaling event is caspase dependent, and caspase-8 activation may be upstream of the mitochondrial events. In summary, we report that QU induced cytochrome c-dependent apoptotic signaling cascade, which may be dependent on its role as a PLA-2 inhibitor. This apoptotic mechanism induced by QU may contribute to its known chemotherapeutic effects.

• 대한토목학회논문집
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• 제37권4호
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• pp.765-773
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• 2017

2015년 효고행동강령이 종료됨에 따라 2015년 3월 개최된 제3차 세계재난위기경감회의에서 센다이프레임워크가 재해위험경감을 위한 새로운 글로벌 아젠다로 채택되었다. 재해위험경감을 위한 글로벌 아젠다에 대한 지속적인 파악은 글로벌 수준뿐만 아니라 국가 수준에서의 재해위험경감에 있어서도 매우 중요하다. 이에 따라 본 연구에서는 재해위험경감을 위한 글로벌 아젠다가 효고행동강령에서 센다이프레임워크로 전환됨에 따라 아젠다의 주요 변화 내용을 살펴보았으며, 효고행동강령 이행에 대한 국내 주요 성과와 센다이프레임워크 채택에 따른 현재까지의 국내 현황을 분석하였다. 또한 센다이프레임워크는 정책결정에 있어서 과학 및 기술의 역할을 강조하고 있으며, 국내에서 과학기술 분야의 연구 동향을 살펴보았다. 재난관리 단계 중 예방을 위한 노력이 49.9%로 가장 많이 이루어지고 있었으며, 행동우선순위 1 (40.4%)과 4 (35.8%) 관련 계획들이 주로 추진되고 있었다. 재난관리를 위한 과학기술 연구 개발 또한 활발하게 진행되고 있는 것으로 분석되었으나, 행동우선순위 4와 관련된 과제가 79.7%로 대부분을 차지하고 있었으며, 4가지 행동우선순위를 모두 고려한 개발이 필요하다고 할 수 있다. 최근 위성기술, 빅데이터 등의 차세대 방재기술을 활용한 재해관리가 요구되고 있으며, 이를 지원할 수 있는 교육 및 정책 수립을 통해 재해위험경감에 효과적으로 기여할 수 있을 것으로 기대된다.

• 대한간호학회지
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• 제26권3호
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• pp.531-543
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• 1996

This study was done to develop a Maternal Identity Scale for Pregnant Women and to test the validity and reliability of the scale. A convenience sample of 161 pregnant women were asked to complete the MISP questionnaire which consisted of 45 item, this was done from December 20, 1995 to January 15, 1996. The research procedure were as follows. The first step was to identify a conceptual definition of maternal identity using Robin(1984)'s maternal identity and maternal experience during pregnancy. The second step was to operationalize the maternal identity, that is, perception of image possible of selves as mother, maternal role play by imagination, and the experiences of various emotional responses which are embedded in the mother-fetus dyad. The third step was item development which resulted in 45 items as appropriate measurement of maternal identity are except for the perception of image possible of selves as mother. The result findings were as follows : 1) Four factors for MISP (finally 40 items) were extracted through the principal component analysis and varimax rotation, and these contributed 49.3% of the variance in the total score. All 40 items in the scale loaded above .43 on one of 4 factors. 2) Each factor was named : factor 1 was named maternal role imagery and has 10 items, factor 2 was named happiness and has 11 items, factor 3 was named maternal fetal interaction and has 10 items, and the last factor 4 was named negative emotion and has 9 items. 3) Cronbach's -alpha coefficient for internal consistsncy was .92 for the total 40 items and .89, .90, .86, .78 for the four subscales in that order. Recommendations are suggested below : 1) The developed MISP be used to assess maternal readiness in pregnancy. 2) Replication study be done to test validity and relaibility. 3) For the overall measure of Maternal Identity in Pregnancy, scale for the perception of image possible of selves as mother, and cognitive domain be reorganized for the maternal identity in pregnancy. 4) It is necessary to identify variables that influences maternal pregnancy. 5) It Is necessary to identify that maternal identity in pregnancy is a reliable index of motherhood, to do correlation studies on maternal identity and major maternal variables in maternal transition period, to reoperationalize the maternal identity in postpartum, and finally to designate a longitudinal study of the maternal identity changes or stabilities.

• Molecules and Cells
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• 제38권2호
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• pp.130-137
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• 2015

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

• Molecules and Cells
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• 제38권12호
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• pp.1064-1070
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• 2015

Translocator protein 18 kDa (TSPO) is a mitochondrial outer membrane protein and is abundantly expressed in a variety of organ and tissues. To date, the functional role of TSPO on vascular endothelial cell activation has yet to be fully elucidated. In the present study, the phorbol 12-myristate 13-acetate (PMA, 250 nM), an activator of protein kinase C (PKC), was used to induce vascular endothelial activation. Adenoviral TSPO overexpression (10-100 MOI) inhibited PMA-induced vascular cell adhesion molecule-1 (VCAM-1) and intracellular cell adhesion molecule-1 (ICAM-1) expression in a dose dependent manner. PMA-induced VCAM-1 expressions were inhibited by Mito-TEMPO ($0.1-0.5{\mu}m$), a specific mitochondrial antioxidants, and cyclosporin A ($1-5{\mu}m$), a mitochondrial permeability transition pore inhibitor, implying on an important role of mitochondrial reactive oxygen species (ROS) on the endothelial activation. Moreover, adenoviral TSPO overexpression inhibited mitochondrial ROS production and manganese superoxide dismutase expression. On contrasts, gene silencing of TSPO with siRNA increased PMA-induced VCAM-1 expression and mitochondrial ROS production. Midazolam ($1-50{\mu}m$), TSPO ligands, inhibited PMA-induced VCAM-1 and mitochondrial ROS production in endothelial cells. These results suggest that mitochondrial TSPO can inhibit PMA-induced endothelial inflammation via suppression of VCAM-1 and mitochondrial ROS production in endothelial cells.

• 법제연구
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• 제44호
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• pp.7-31
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• 2013

글로벌 경제의 성장 패러다임이 창조경제 체제로 변화하고 있다. 이러한 변화가 가져올 결과를 제대로 이해하기 위해서 나아가 바람직한 미래의 창조경제를 위해서, 그리고 ICT의 역할은 무엇인지에 대해서 살펴보고자 한다. 창조경제는 경제성장론에 바탕을 두고 있다. 신경제 체제에서 ICT 부문에 대한 투자를 통해 인플레이션 없는 지속적 성장 또는 주어진 인플레이션 하에서 보다 높은 수준의 지속 가능한 성장을 달성할 수 있게 된다. 그러나 미국에서와 달리 국내에서는 신경제 현상이 유의하게 나타나지 않았다. 신경제보다는 요소투입에 의존하는 성장 패턴을 보였기 때문이다. ICT는 신경제(디지털 경제)로의 이행을 위한 필수적 요소이긴 하나 그 자체로 생산성 및 성장을 전부 설명하는 것은 아니라고 평가된다. 관건은 어떻게 정보통신 및 기술발전을 타 산업에 확산시키는지의 여부에 달려 있다. ICT는 그 자체가 창조산업이거나 창조경제가 아니라 enabler로서 제대로 역할을 할 수 있어야 한다. 창조경제는 신경제론의 연장선에 볼 수도 있다. 자본과 노동력 요소 투자 이외에 문화적 자산과 인적 자원(Human resource)을 투여하여 가치를 생산해 내는 경제를 의미한다. 그러나 투입요소의 변화에 국한되면, 진정한 의미의 창조경제를 기대하기 어렵다. 그러한 의미에서 창조경제는 정권 5년 정책이슈가 아니라 미래지향적이어야 하며 단순히 투입요소에 창의력이 추가되는 차원을 넘어 through-put이라고 할 시스템의 변화가 수반되어야 한다. 여기에 ICT의 역할이 있다.

• Journal of Ginseng Research
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• 제47권4호
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• pp.534-542
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• 2023

Background: Ginsenoside Rg1, a bioactive component of Ginseng, has demonstrated anti-inflammatory, anti-cancer, and hepatoprotective effects. It is known that the epithelial-mesenchymal transition (EMT) plays a key role in the activation of hepatic stellate cells (HSCs). Recently, Rg1 has been shown to reverse liver fibrosis by suppressing EMT, although the mechanism of Rg1-mediated anti-fibrosis effects is still largely unclear. Interestingly, Smad7, a negative regulator of the transforming growth factor β (TGF-β) pathway, is often methylated during liver fibrosis. Whether Smad7 methylation plays a vital role in the effects of Rg1 on liver fibrosis remains unclear. Methods: Anti-fibrosis effects were examined after Rg1 processing in vivo and in vitro. Smad7 expression, Smad7 methylation, and microRNA-152 (miR-152) levels were also analyzed. Results: Rg1 significantly reduced the liver fibrosis caused by carbon tetrachloride, and reduced collagen deposition was also observed. Rg1 also contributed to the suppression of collagenation and HSC reproduction in vitro. Rg1 caused EMT inactivation, reducing Desmin and increasing E-cadherin levels. Notably, the effect of Rg1 on HSC activation was mediated by the TGF-β pathway. Rg1 induced Smad7 expression and demethylation. The over-expression of DNA methyltransferase 1 (DNMT1) blocked the Rg1-mediated inhibition of Smad7 methylation, and miR-152 targeted DNMT1. Further experiments suggested that Rg1 repressed Smad7 methylation via miR-152-mediated DNMT1 inhibition. MiR-152 inhibition reversed the Rg1-induced promotion of Smad7 expression and demethylation. In addition, miR-152 silencing led to the inhibition of the Rg1-induced EMT inactivation. Conclusion: Rg1 inhibits HSC activation by epigenetically modulating Smad7 expression and at least by partly inhibiting EMT.

• International Journal of Computer Science & Network Security
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• 제22권1호
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• pp.340-346
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• 2022

The article examines the formation of the scientific paradigm of transformation of the potential-forming space of the regional economy under the influence of intellectual and innovative determinants. Based on the study of different scientific views on the nature and properties of potential-forming space through the study of approaches to understanding the concept of "space" clarified the complexity and multifaceted nature of the phenomenon and found that its characteristics are relevant to the industrial development model. It is revealed that the leading modern trends related to the spread of globalization and regionalization, rapid development of information and communication technologies, diffusion of innovations accompany the transition from industrial to post-industrial development and its development, which leads to new development: changes production, nature and relations between business entities, etc. It is proved that under such conditions, the region as a key element of the economic system, acquires a leading role in achieving sustainable and balanced development. These processes significantly affect the potential-forming space of the regional economy under the influence of intellectual and innovative determinants, leading to the need for its transformation and change in accordance with modern realities, which is reflected in thorough research on the formation of scientific paradigm based on the formation of its theoretical foundations and methodological basis. This study reveals the essence, role, functions, structure, process of formation of the scientific paradigm of transformation of the potential-forming space of the regional economy under the influence of intellectual and innovative determinants. It is proved that the formation of the modern scientific paradigm of transformation of the potential-forming space of the regional economy under the influence of intellectual and innovative determinants occurs in the context of building a post-industrial model of development, accompanied by consideration of the region as a spatial object territories from the physical plane to the spatial environment in which the development of human capital, innovation and self-development of the region. Taking into account the above, the article outlines the prerequisites and factors of formation of the scientific paradigm of transformation of the potential-forming space of the regional economy under the influence of intellectual and innovative determinants.

• 융합보안논문지
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• 제24권2호
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• pp.167-174
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• 2024

현대전에서 예비전력은 매우 중요한 역할을 한다. 그동안 예비전력에 대한 주 관심 대상은 남성이었고, 여군 예비전력은 거의 관심을 받지 못해 왔다. 이제는 여러 상황 변화에 따라 여군 예비전력의 필요성에 관심을 가져야 할 때이다. 인구감소에 따라 상비군 전력 자원뿐 아니라 예비전력의 자원 감소, 전쟁 양상변화에 따른 여군이 장점을 가지고 임무수행할 수 있는 분야의 발전, 그리고 양성평등 및 여성의 사회적 역할 증대의 시대적 상황 변화가 주요한 요인이라고 볼 수 있다. 군에서 현역 여군이 증가하는 이유이기도 하다. 그러나, 현재 전역 여군은 예비군에 편입되는 것이 필수가 아닌 선택사항이다. 예비군 편입을 희망하는 퇴역 여군 수가 증가하지 않는 경우, 현역 여군 수가 증가함에 따라 예비군에 편입되는 간부의 수는 감소하게 될 수 있다. 이에 본 연구의 목적은 전역 여군의 예비역 편입 확대 필요성과 현역 여군의 증가가 예비역 여군 전력에 미치는 영향을 연구하는 것이다.