• Reproductive and Developmental Biology
• /
• v.36 no.4
• /
• pp.237-241
• /
• 2012

Embryonic genome activation (EGA) is the first major transition that occurs after fertilization, and entails a dramatic reprogramming of gene expression that is essential for continued development. Although it has been suggested that EGA in porcine embryos starts at the four-cell stage, recent evidence indicates that EGA may commence even earlier; however, the molecular details of EGA remain incompletely understood. The RNA polymerase II of eukaryotes transcribes mRNAs and most small nuclear RNAs. The largest subunit of RNA polymerase II can become phosphorylated in the C-terminal domain. The unphosphorylated form of the RNA polymerase II largest subunit C-terminal domain (IIa) plays a role in initiation of transcription, and the phosphorylated form (IIo) is required for transcriptional elongation and mRNA splicing. In the present study, we explored the nuclear translocation, nuclear localization, and phosphorylation dynamics of the RNA polymerase II C-terminal domain in immature pig oocytes, mature oocytes, two-, four-, and eight-cell embryos, and the morula and blastocyst. To this end, we used antibodies specific for the IIa and IIo forms of RNA polymerase II to stain the proteins. Unphosphorylated RNA polymerase II stained strongly in the nuclei of germinal vesicle oocytes, whereas the phosphorylated form of the enzyme was confined to the chromatin of prophase I oocytes. After fertilization, both unphosphorylated and phosphorylated RNA polymerase II began to accumulate in the nuclei of early stage one-cell embryos, and this pattern was maintained through to the blastocyst stage. The results suggest that both porcine oocytes and early embryos are transcriptionally competent, and that transcription of embryonic genes during the first three cell cycles parallels expression of phosphorylated RNA polymerase II.

• Cartoon and Animation Studies
• /
• s.33
• /
• pp.271-291
• /
• 2013

Cartoons exhibit contents and images and deliver them to readers by repeating frames. Frames embody the cross sectional factors of space-time, and the repetition of frames makes those cross sectional factors into a continuous flow. The gutter, which exists between frames, takes the role to make the continuous flow link smoothly, and at the beginning stage of the gutter, the operating of the gutter was usually passive by the frame meanings. However, the gutter is not just a medium for linking the frames, but is an efficient participation device for creating new expression methods of cartoon organization. By producing new directing methods for cartoon organization, the distinctive functions of the gutter have been kept alive with cartoon history. The distinctive functions of the gutter can be divided largely into transition function, reminiscence function, speech balloon function, pen function, and decorative function. And those functions perform actively as direct expression method of the gutter. When those functional methods are expressed by the author's intention and the frequency of them is generalized according to described contents, using each author's individual differences can suggest direction for new directing organization of cartoons.

• Bulletin of the Korean Chemical Society
• /
• v.29 no.6
• /
• pp.1149-1156
• /
• 2008

The conformational study on neutral and zwitterionic L-alanines (N-Ala and Z-Ala, respectively) and the transition state (TS) for their interconversion is carried out using ab initio HF and density functional B3LYP methods with the self-consistent reaction field method in the gas phase and in solution. At both the HF and B3LYP levels of theory, the local minimum N1 for N-Ala is found to be most preferred in the gas phase and a weak asymmetric bifurcated hydrogen bond between the amino hydrogens and the carbonyl oxygen appears to play a role in stabilizing this conformation. The local minima N2a and N2b are found to be the second preferred conformations, which seem to be stabilized by a hydrogen bond between the amino nitrogen and the carboxylic hydrogen. The relative stability of the local minimum N2b is remarkably increased in solution than that in the gas phase. The local minimum N2b becomes more stable than the local minimum N2a in most of the solution. On the whole the relative free energies of Z-Ala and TS become more lowered, as the solvent polarity increases. N-Ala prevails over Z-Ala in aprotic solutions but Z-Ala is dominantly populated in ethanol and water. In aprotic solutions, the population of Z-Ala increases somewhat with the increase of solvent polarity. The barrier to Z-Ala-to-N-Ala interconversion increases on the whole with the increase of solvent polarity, which is caused by the increase of stability for Z-Ala.

• Journal of the Korean Chemical Society
• /
• v.63 no.6
• /
• pp.427-434
• /
• 2019

The role of hydrophobic residues on protein folding reaction was studied by folding kinetics measurements in conjunction with protein engineering. The HubWA, which was derived from human ubiquitin by mutating the residues at 45 (Phe to Trp) and 26 (Val to Ala), was used as a mutational background. Fourteen hydrophobic residues were mutated to alanine. Among fourteen variants generated, only four variant proteins (V5A, I13A, V17A, and I36A) were suitable for folding study. The folding kinetics of these variants was measured by stopped-flow fluorescence spectroscopy. The folding kinetics of HubWA and V17A was observed to follow a three-state on-pathway mechanism. On the other hand, folding kinetics of V5A, I13A, and I36A was observed to follow a two-state mechanism. Based on these observations, transition of protein folding reaction from collision-diffusion mechanism to nucleation-condensation mechanism was discussed.

• Journal of Contemporary Eastern Asia
• /
• v.19 no.1
• /
• pp.43-58
• /
• 2020

This study examines media used for information in the East Asian countries of China, Japan, South Korea, Taiwan, and Singapore, using data from the World Values Survey. The sharing of Confucian culture may lead to a uniform media structure across these nations. Another possibility is technological determinism, which would also lead to similarity across nations. However, it is possible that countries are at different stages of technology development and will eventually become more similar. An opposing notion is that differences in other values among nations predict digital media use. To examine the evidence considering these possibilities, we factor analyze each population's use of nine traditional and digital media to see how similar the structures are. What results is a three-dimensional solution for four out of five countries, except Singapore, which has a more simple two-dimensional structure. Analysts regard Singapore as the most digitally connected society, which raises the question as to whether it is higher on a technological development trajectory, to which other countries may transition. Perhaps a more simple media use structure is an adaptation to increasing information load. As well, as mobile devices have become a primary means of accessing the range of traditional and social media, it may have an expanded role in reducing media channel entropy. In terms of frequency of media use, Singapore is highest, while China is the lowest. Singapore stands out in high mobile use, and China for low Internet use. There appear to be developmental differences across the nations. Regressions on Internet use for 18 values indices find different values predictors in the East Asian countries, ruling out Confucianism as producing similar media patterns.

• Journal of Families and Better Life
• /
• v.34 no.4
• /
• pp.89-109
• /
• 2016

The purposes of this study were to analyze and understand the life of mothers rearing children with Down syndrome and to present basic data for overcoming the difficulties of those families. For these purposes, this study made use of the phenomenological analysis and approach with a qualitative research method. The subjects of this study are 4 mothers rearing children with Down syndrome who are beyond school age. The results were as follows. First, the reason why mothers with Down syndrome children went through psychological shock and horror was the social stigma due to the unusual appearance of children with Down syndrome. Second, the isolation with in the public education system was disclosed as a factor causing much sorrow and pain. Through this, we can recognize the importance of ensuring the equal right to receive education of children with Down syndrome. Third, the period which the mother experienced most shock and confusion was right after finding out about the disability that her child has. So, we can identify the importance of early intervention providing psychotherapy and rearing service to the parents. Fourth, the crucial factor that relieved pain and pressure from mothers was social support including family. Therefore, the social support system for mothers with Down syndrome children has to be developed and strengthened. Fifth, the crucial factor that made mother grow up during rearing experience was the reestablishment of cognition about the desired role of mothers. Through this, we can identify the importance of the mental transition process to independent thinking.

• Journal of Digital Convergence
• /
• v.16 no.8
• /
• pp.161-167
• /
• 2018

The purpose of this study is to investigate how the advertising campaign is spreading in social networks, and how the advertising model plays an important role in advertisement diffusion. In order to grasp the diffusion patterns of advertising, a text mining and social network analysis were conducted using the beer brand 'Kloud' as a collection keyword. After analyzing the social data for two months since the on-air of 'Good Body' advertisement, which was the first ad that "Sulhyun" appeared in. After the launch of the ad, Kloud has been mainly associated with keywords such as 'yavis & trendy style', 'beer brand', 'beer matching food', 'luxury beer drinking place', 'leisure trend', and 'SNS activity', etc. In addition, "Sul Hyun" also showed that an advertising model contributes to the spread of advertisement on social media in terms of image transition as well as brand's name and unique selling point.

• Tuberculosis and Respiratory Diseases
• /
• v.38 no.4
• /
• pp.347-356
• /
• 1991

Endobronchial tuberculosis is a serious disase because it frequently leaves airway obstruction as the complication, and the treatment of airway obstruction is generally troublesome. In the tumorous type of endobonchial tuberculosis, the bronchial patency is partially or completely compromised with lymph node contents when the necrotic focus of the lymph node ruptures into the bronchial lumen to form a bronchoglandular fistula. To investigate the transition of endobronchial lesion and to evaluate the therapeutic role of bronchoscopic electrocautery in the tumorous type of endobronchial tuberculosis, we performed electrocautery in addition to the combination chemotherapy with steroid and anti-tuberculous drugs in two cases which had airway obstruction proximal to lobar bronchus with the impairment of pulmonary function. We also treated another two cases only with chemotherapy and we have followed up four cases over a 36-month period. In cases that bronchoscopic electrocautery was done, the bronchial patency was completely restored and the impairment of pulmonary function disappeared just after cautery and these effects have remained for 12 months or more. But in cases of medical treatment only, bronchial stenosis was inevitable as the tumorous type of endobronchial tuberculosis changed to the stenotic type with fibrosis. It can be concluded that bronchoscopic electrocautery can nip the occurence of bronchial stenosis in the bud when it is applied in addition to combination chemotherapy with steroid and antituberculous drugs in the tumorous type of endobronchial tuberculosis.

• Korean Journal of Clinical Laboratory Science
• /
• v.49 no.1
• /
• pp.40-47
• /
• 2017

Granulosa cells, which surround the oocyte within the ovarian follicle, play an essential role in creating conditions required for the development of oocytes and follicles. The solute carrier family (SLC) is comprised of influx transporters of steroidal hormones, various drugs, and several other substrates. The differential expression of selected DEGs was confirmed using in situ hybridization analysis. SLC23A3 and SLC39A10 were highly expressed in the ovary. The SLC39A10 gene was expressed in the primordial follicle stage, but SLC23A3 was expressed in the growing follicle stage. Contrastingly, the expression of SLC23A3 was increased in granulosa cells at the growing follicle stage. The differential expressions of SLC23A3 and SLC39A10 between the primordial and primary follicles were additionally confirmed by using follicle isolations. The gene expression profile from the present study may provide insight for future studies on the mechanism(s) involved in primordial-primary follicular transition and suggestions to promote follicular development in ovarian dysfunction.

• Journal of the military operations research society of Korea
• /
• v.17 no.2
• /
• pp.1-15
• /
• 1991

If one were to say that war is political in its nature, then war could be one of the several ways of achieving political goals. Thus, the aspect of wars will, in certainty, be altered by the changes in political environment. Wars in the past broke out mainly due to ideological differences that ran to extremes and were of high intensity. However, wars today show such restraints as controlling them beforehand or during the war in anticipation of the post-war situation. The trend of ideas to actualize such wars in the effective operation of airpower is on the rise. Airpower normally possesses speed and operative flexibility as well as capability of destruction, so it is possible to destroy the politically declared targets clearly. Previous airpower was merely employed as means of helping to vertically detouring the spatial obstacle that the ground forces encountered. Over the years, due to the speedy improvement of aviation technology and of ideas concerning wars in space, more advanced application has been developed. but they also were nothing more than an auxiliary role to facilotate the ground forces, that needed a longer effective range of firepower and did not become forces with the right of self-determination, that is, the fact of decisive war that makes its outcome. However, under transitional strategic environment like that of the present, Airpower possesses not only the capability to operate as a decisive means of war but also as theories to support it. The advancement in air technology has enabled supremacy over targets in depth, and the development of electronic technology has empowered the improvement of degree of destruction but also triumphant war by means of an overwhelming supremacy in a relatively short period. Thus, the method of systematic destruction that accomplishes the stated goals while rejecting the damage of accumulative destruction has been realized. The progress of such a concept has also proved that the counter-force strategy that has been developed in nuclear strategy is useful in conventional warfare as well. Therefore, it can be said that the under today's strategic environment airpower is an outstanding means of military strategy that can deeply affect to achieve the national objectives.