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Race- and Isolate-specific Molecular Marker Development through Genome-Realignment Enables Detection of Korean Plasmodiophora brassicae Isolates, Causal agents of Clubroot Disease

  • Jeong, Ji -Yun;Robin, Arif Hasan Khan;Natarajan, Sathishkumar;Laila, Rawnak;Kim, Hoy-Taek;Park, Jong-In;Nou, Ill-Sup
    • The Plant Pathology Journal
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    • v.34 no.6
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    • pp.506-513
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    • 2018
  • Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot disease is caused by the obligate parasite Plasmodiophora brassicae, which is difficult to study because it is nonculturable in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae isolates, but none of those markers was either race-specific or pathotype-specific. Our current study aimed to develop race- and isolate-specific markers by exploiting genomic sequence variations. A total of 119 markers were developed based on unique variation exists in genomic sequences of each of the races. Only 12 markers were able to detect P. brassicae strains of each isolate or race. Ycheon14 markers was specific to isolates of race 2, Yeoncheon and Hoengseong. Ycheon9 and Ycheon10 markers were specific to Yeoncheon isolate (race 2, pathotype 3), ZJ1-3, ZJ1-4 and ZJ1-5 markers were specific to Haenam2 (race 4) isolate, ZJ1-35, ZJ1-40, ZJ1-41 and ZJ1-49 markers were specific to Hoengseong isolate and ZJ1-56 and ZJ1-64 markers were specific to Pyeongchang isolate (race 4, pathotype 3). The PCR-based sequence characterized amplified region (SCAR) markers developed in this study are able to detect five Korean isolates of P. brassicae. These markers can be utilized in identifying four Korean P. brassicae isolates from different regions. Additional effort is required to develop race- and isolate-specific markers for the remaining Korean isolates.

Enhanced pig production: potential use of insect gut microbiota for pig production

  • Shin, Jiwon;Kim, Bo-Ra;Guevarra, Robin B.;Lee, Jun Hyung;Lee, Sun Hee;Kim, Young Hwa;Wattanaphansak, Suphot;Kang, Bit Na;Kim, Hyeun Bum
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.655-663
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    • 2018
  • The insect gut microbiome is known to have important roles in host growth, development, digestion, and resistance against pathogens. In addition, the genetic diversity of the insect gut microbiota has recently been recognized as potential genetic resources for industrial bioprocessing. However, there is limited information regarding the insect gut microbiota to better help us understand their potential benefits for enhanced pig production. With the development of next-generation sequencing methods, whole genome sequence analysis has become possible beyond traditional culture-independent methods. This improvement makes it possible to identify and characterize bacteria that are not cultured and located in various environments including the gastrointestinal tract. Insect intestinal microorganisms are known to have an important role in host growth, digestion, and immunity. These gut microbiota have recently been recognized as potential genetic resources for livestock farming which is using the functions of living organisms to integrate them into animal science. The purpose of this literature review is to emphasize the necessity of research on insect gut microbiota and their applicability to pig production or bioindustry. In conclusion, bacterial metabolism of feed in the gut is often significant for the nutrition intake of animals, and the insect gut microbiome has potential to be used as feed additives for enhanced pig performance. The exploration of the structure and function of the insect gut microbiota needs further investigation for their potential use in the swine industry particularly for the improvement of growth performance and overall health status of pigs.

Intestinal Microbial Dysbiosis in Beagles Naturally Infected with Canine Parvovirus

  • Park, Jun Seok;Guevarra, Robin B.;Kim, Bo-Ra;Lee, Jun Hyung;Lee, Sun Hee;Cho, Jae Hyoung;Kim, Hyeri;Cho, Jin Ho;Song, Minho;Lee, Ju-Hoon;Isaacson, Richard E.;Song, Kun Ho;Kim, Hyeun Bum
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1391-1400
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    • 2019
  • Canine parvoviral enteritis (PVE) is an important intestinal disease of the puppies; however, the potential impact of the canine parvovirus (CPV) on the gut microbiota has not been investigated. Therefore, the aim of this study was to evaluate the gut microbial shifts in puppies naturally infected with CPV. Fecal samples were collected from healthy dogs and those diagnosed with PVE at 4, 6, 8, and 12 weeks of age. The distal gut microbiota of dogs was characterized using Illumina MiSeq sequencing of the bacterial 16S rRNA genes. The sequence data were analyzed using QIIME with an Operational Taxonomic Unit definition at a similarity cutoff of 97%. Our results showed that the CPV was associated with significant microbial dysbiosis of the intestinal microbiota. Alpha diversity and species richness and evenness in dogs with PVE decreased compared to those of healthy dogs. At the phylum level, the proportion of Proteobacteria was significantly enriched in dogs with PVE while Bacteroidetes was significantly more abundant in healthy dogs (p < 0.05). In dogs with PVE, Enterobacteriaceae was the most abundant bacterial family accounting for 36.44% of the total bacterial population compared to only 0.21% in healthy puppies. The two most abundant genera in healthy dogs were Prevotella and Lactobacillus and their abundance was significantly higher compared to that of dogs with PVE (p < 0.05). These observations suggest that disturbances of gut microbial communities were associated with PVE in young dogs. Evaluation of the roles of these bacterial groups in the pathophysiology of PVE warrants further studies.

MSHR-Aware Dynamic Warp Scheduler for High Performance GPUs (GPU 성능 향상을 위한 MSHR 활용률 기반 동적 워프 스케줄러)

  • Kim, Gwang Bok;Kim, Jong Myon;Kim, Cheol Hong
    • KIPS Transactions on Computer and Communication Systems
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    • v.8 no.5
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    • pp.111-118
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    • 2019
  • Recent graphic processing units (GPUs) provide high throughput by using powerful hardware resources. However, massive memory accesses cause GPU performance degradation due to cache inefficiency. Therefore, the performance of GPU can be improved by reducing thread parallelism when cache suffers memory contention. In this paper, we propose a dynamic warp scheduler which controls thread parallelism according to degree of cache contention. Usually, the greedy then oldest (GTO) policy for issuing warp shows lower parallelism than loose round robin (LRR) policy. Therefore, the proposed warp scheduler employs the LRR warp scheduling policy when Miss Status Holding Register(MSHR) utilization is low. On the other hand, the GTO policy is employed in order to reduce thread parallelism when MSHRs utilization is high. Our proposed technique shows better performance compared with LRR and GTO policy since it selects efficient scheduling policy dynamically. According to our experimental results, our proposed technique provides IPC improvement by 12.8% and 3.5% over LRR and GTO on average, respectively.

Fecal microbiome shifts by different forms of copper supplementations in growing pigs

  • Kim, Minji;Cho, Jae Hyoung;Seong, Pil-Nam;Jung, Hyunjung;Jeong, Jin Young;Kim, Sheena;Kim, Hyeri;Kim, Eun Sol;Keum, Gi Beom;Guevarra, Robin B.;Kim, Hyeun Bum
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1386-1396
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    • 2021
  • Copper is an essential mineral for pigs, thus it is used as a feed additive in the forms of copper sulfate. Therefore, this study aimed at characterizing the fecal microbiota shifts in pigs as fed by different forms of copper supplementation. 40 growing pigs aged 73 ± 1 days with an average weight of 30.22 ± 1.92kg were randomly divided into 5 groups. The control group (CON) fed with basal diet, while treatment groups were fed a basal diet supplemented with 100 ppm/kg of copper sulfate (CuSO4), Cu-glycine complex (CuGly), Cu-amino acid complex (CuAA), and Cu-hydroxy(4methylthio)butanoate chelate complex (CuHMB) for 28 days of trial, respectively. The data presented the comparison between inorganic and organic copper supplementation through gut microbiota in growing pigs. Alpha and Beta diversity anaylsis resulted in copper supplementation did shifted gut microbioal community structure. At the phylum level, Firmicutes and Bacteroidetes were the most abundant phyla at all times regardless of treatment. At the genus level, the relative abundances of Prevotella, Lactobacillus, Megasphaera, and SMB53 of the CuGly and CuHMB groups were significantly higher than those of copper sulfate and basal diet groups. Overall, this study may provide the potential role of organic copper replacing inorganic copper, resulting in increased beneficial bacteria in the pig gut.

A Study on the Quality Deviation of High-Strength Concrete from Multiple Ready Mixed Concrete Companies (다수 레미콘사에서 납품된 콘크리트 품질 편차에 관한 연구)

  • Park, Dong-Cheon;Seok, Won-Kyun;Jeon, Hyun-Soo;Kim, Young-Sun
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.6
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    • pp.577-583
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    • 2022
  • On large-scale sites, concrete is often delivered from a number of ready-mixed concrete companies, but even if the same concrete mixture table is used, it is thought that there will be a difference in quality due to differences in materials and manufacturing equipment. Due to a lack of previous research in this area, this study measured the properties of fresh concrete, compressive strength, and chlorine ion diffusion coefficient using the concrete supplied by 12 ready-mixed companies in Busan. The fresh concrete properties met the criteria. The compressive strength increased by 137% for 30MPa, 131% for 45MPa, and 117% for 80MPa by specified compressive strength. For the chlorine ion diffusion coefficient, the average value for each specified compressive strength could be derived without significant variation. The higher the compressive strength, the greater the deviation , and the lower the compressive strength, the greater the deviation in the chlorine ion diffusion coefficient.

Evaluation of Liquefaction Model using Dynamic Centrifuge Test (포화된 경사 사질토 지반의 액상화 수치모델 거동평가)

  • Lee, Jin-Sun;Lee, Sang-Un
    • Journal of the Korean Geotechnical Society
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    • v.38 no.11
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    • pp.31-42
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    • 2022
  • This study verified numerical analysis of the liquefaction phenomenon using LEAP-2017 international round-robin centrifuge test results. Dynamic centrifuge test is performed by applying a 1 Hz tapered sine wave to the model soil deposit, which was formed under a water table in a surface slope of 5° using Ottawa F-65 sand. A numerical model was made on a prototype scale and analyzed using the finite difference method in 2D and 3D conditions. The analyses were verified for acceleration and pore-water pressure histories with depth and residual displacement. Verification results revealed that all numerical liquefaction models agree reasonably with the test result for acceleration histories but not for pre-water pressure histories. Numerical analyses showed much smaller residual displacement than the centrifuge test. Thus, it is necessary to compare the results of numerical analysis with the centrifuge test performed by other institutes in the future.

Identification of System Frequency Variations in Vehicle-Bridge Interaction Systems (교량-차량 동적상호작용을 고려한 시간가변적 시스템 특성 분석)

  • Lee, Jaehun;Lee, Young Jae;Kim, Robin Eunju
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.35 no.1
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    • pp.23-28
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    • 2022
  • Natural frequency variations in a vehicle-bridge interaction system is examined. The interaction system is designed for a simple beam subject to a moving vehicle. The equation of motion for the system is derived under the quarter-car condition, and numerical simulation is performed. Frequency amplification ratio (FAR) is defined as a ratio between the initial and the varying natural frequency of the system; a discontinuity in the FAR implies a resonance condition. Analysis is mainly focused on patterns, frequency variation characteristics, and discontinuity points of the FAR under the vehicle mass and tire stiffness variations. The result reveals that the interactions between the system affects the natural frequency of both the vehicle and the bridge in similar frequency regions that can be visually identified at the middle of the span using the FAR.

A History-based Scheduler for Dynamic Load Balancing on Distributed VOD Server Environments (분산 VOD 서버 환경에서 히스토리 기반의 동적 부하분산 스케줄러)

  • Moon, Jongbae
    • Proceedings of the Korea Information Processing Society Conference
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    • 2010.04a
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    • pp.210-213
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    • 2010
  • 최근 사용자의 멀티미디어에 대한 요구의 증가가 VOD (Video-on-Demand) 서비스를 발전시키게 되었다. VOD는 엔터테인먼트나 원격 교육, 광고 및 정보 등 많은 분야에서 사용되고 있다. 이러한 VOD 서비스는 많은 디스크 I/O와 네트워크 I/O를 요구하며 기존 웹 서버 시스템과 비교했을 때 오랜 시간동안 서비스를 해야 하는 특징을 가지고 있다. 또한 VOD 서비스는 많은 네트워크와 디스크의 대역폭을 요구하며, 서비스의 QoS에 민감해서 사용자 응답시간이 길어지면 사용자 요청의 취소율이 높아지게 된다. 따라서 불만족스러운 서비스의 증가로 네트워크 부하만 증가하게 된다. 이러한 기존 웹 서버 환경과는 다른 부하의 패턴이 있는 VOD 서비스 환경에서는 부하를 균형적으로 분배하여 서비스의 QoS를 높이는 것이 매우 중요하다. 본 논문에서는 분산 VOD 시스템 환경에서 부하를 효율적으로 분산하기 위해 계층형 분산 VOD 시스템 모델과 사용자 요청 패턴의 히스토리와 유전 알고리즘을 기반으로 한 스케줄러를 제안한다. 본 논문에서 제안한 계층형 분산 VOD 시스템 모델은 서버들을 지역적으로 분산하고 제어 서버를 지역마다 설치하여 지역에 있는 VOD 서버들을 관리하도록 구성한다. 사용자 요청을 지역 서버군 내에서 분산시키기 위해서 히스토리를 기반으로 한 유전 알고리즘을 사용한다. 이러한 히스토리 정보를 기반으로 유전 알고리즘의 적합도 함수에 적용하여 VOD 시스템을 위한 유전 알고리즘과 유전 연산을 구현한다. 본 논문에서 제안한 부하 분산 알고리즘은 VOD 서비스 환경에서 사용자 요구에 대한 부하를 보다 정확하게 예측하여 부하를 분산할 수 있다. 본 논문에서 제안한 계층형 분산 VOD 시스템의 부하 분산 알고리즘의 성능을 테스트하기 위해 OPNET 기반 시뮬레이터를 구현한다. 라운드로빈(round-robin) 방식과 랜덤(random) 방식과의 비교 실험을 통해 본 논문에서 제안한 부하 분산 알고리즘의 성능을 평가한다. 비교 실험을 통해 본 논문에서 제안한 알고리즘이 보다 안정적인 QoS를 제공하는 것을 보여준다.

Estimation of maximum object size satisfying mean response time constraint in web service environment (웹 서비스 환경에서 평균 응답 시간의 제약조건을 만족하는 최대 객체 크기의 추정)

  • Yong-Jin Lee
    • Journal of Internet of Things and Convergence
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    • v.9 no.3
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    • pp.1-6
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    • 2023
  • One of the economical ways to satisfy the quality of service desired by the user in a web service environment is to adjust the size of the object. To this end, this study finds the maximum size of objects that satisfy this constraint when the mean response time is given below an arbitrary threshold for quality of service. It can be inferred that in the steady state of system, the mean response time in the deterministic model by using the round-robin will be the same as that of the queueing model following the general distribution. Based on this, analytical formulas and procedures for finding the maximum object size are obtained. As a service distribution of web traffic, the Pareto distribution is appropriate, so the maximum object size is computed by applying the M/G(Pareto)/1 model and the M/G/1/PS model using exponential distribution as computational experience. Performance evaluation through numerical calculation shows that as the shape parameter in the Pareto distribution increases, the M/G(Pareto)/1 model and M/G/1/PS model have the same maximum object size. The results of this study can be used to environments where objects can be sized for economical web service control.