• 한국식품위생안전성학회지
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• 제24권2호
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• pp.148-153
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• 2009

본 연구에서는 심혈관계 질환의 표지인자인 콜레스테롤의 농도를 새롭게 개발한 형광 크로마토그라피 방법에 의하여 측정 하고자 시도하였다. 개발된 측정 시스템은 크로마토그라피 스트립이 들어 있는 카트리지, 발색제 AEC, 콜레스테롤 옥사데이즈 (Cholesterol Oxidase, CO), 콜레스테롤 에스트라아제(Cholesterol Esterase, CE), 호스래 디쉬페록시다아제 (Horseradish peroxidase, HRP)를 포함하는 효소혼합 수용액, 그리고 형광 판독기로 구성되어 었다. 콜레스테롤 농도 변화에 따른 형광 세기 변화로 얻은 상관계수 r= 0.968로 측정 가능한 농도 범위에서 신뢰할 만한 직선성 관계를 제시하여 주었다. 회복률과 Hitachi 747 검사기기와 비교 테스트에서는 각각 106.5-94%와 상관관계 r = 0.939 (p<0.001)로 비교적 높은 유의성을 보여주었다. 따라서 본 연구에서 개발된 새로운 콜레스테롤 농도 측정법은 빠른 반응 시간 (8분)과 적은 시료량 ($5\;{\mu}l$)을 사용하기에 시료를 실험실로 운반할 필요없이 클리닉에서 측정 가능한 준현장검사 플랫폼형으로 개발되었으며 기존의 고가의 자동화 장비를 사용하는 생화학적 진단방식 대체용으로의 응용이 기대된다.

• Tuberculosis and Respiratory Diseases
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• 제83권3호
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• pp.175-184
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• 2020

Quantitative sputum cytometry facilitates in assessing the nature of bronchitis associated with exacerbations of chronic obstructive pulmonary disease (COPD). This is not assessed in most clinical trials that evaluate the effectiveness of strategies to prevent or to treat exacerbations. While up to a quarter of exacerbations may be associated with raised eosinophil numbers, the vast majority of exacerbations are associated with neutrophilic bronchitis that may indicate airway infections. While eosinophilia may be a predictor of response to corticosteroids (oral and inhaled), the limited efficacy of anti-interleukin 5 therapies would suggest that eosinophils may not directly contribute to those exacerbations. However, they may contribute to airspace enlargement in patients with COPD through various mechanisms involving the interleukin 13 and matrix metalloprotease pathways. The absence of eosinophils may facilitate in limiting the unnecessary use of corticosteroids. The presence of neutrophiia could prompt an investigation for the specific pathogens in the airway. Additionally, sputum measurements may also provide insight into the mechanisms of susceptibility to airway infections. Iron within sputum macrophages, identified by hemosiderin staining (and by more direct quantification) may impair macrophage functions while the low levels of immunoglobulins in sputum may also contribute to airway infections. The assessment of sputum at the time of exacerbations thus would facilitate in customizing treatment and treat current exacerbations and reduce future risk of exacerbations.

• 분석과학
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• 제17권4호
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• pp.347-354
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• 2004

본 연구에서는 낙동강과 그 유역에 서식하는 붕어와 황소개구리 체내의 polychlorinated biphenyls (PCBs)의 축적도와 분포특성을 조사하였다. 낙동강의 구미부터 하구언까지 모두 5개 지점에서 붕어와 황소개구리를 채집하였으며, 이들의 근육 부분을 최종 분석시료로 하였다. PCBs의 정량분석은 GC-MSD 시스템으로 처리하였으며, PCBs 총농도를 대변할 수 있는 62개 congener를 분석항목으로 선정하였다. 붕어에서는 모두 24개의 congener가 검출되었고 총 PCBs 농도는 0.74~5.41 ng/g wet weight 범위로 나타났다. 황소개구리에서는 낙동강 하구언 1개 지점에서 단지 2개의 congener가 검출되었고 그 농도도 0.24 ng/g wet weight로서 붕어보다 약 22배 정도 낮게 검출되었다. 전체적으로, PCB 153이 가장 높게 검출된 congener이고, penta- 및 hexa-CBs가 총 PCBs의 78%를 차지하였다.

• Mass Spectrometry Letters
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• 제10권2호
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• pp.43-49
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• 2019

The aim of this study was conducted to develop an analytical method to determine the concentration of ceftiofur residue in eel, flatfish, and shrimp. For derivatization and extraction, the sample was hydrolyzed with dithioerythritol to produce desfuroylceftiofur, which was then derivatized by iodoacetamide to obtain desfuroylceftiofur acetamide. For purification, the process of solid phase extraction (Oasis HLB) was used. The target analytes were confirmed and quantified in $C_{18}$ column using liquid chromatography-tandem mass spectrometry with 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) as the mobile phase. The linearity of the standard calibration curve was confirmed by a correlation coefficient, $r^2>0.99$. The limit of quantification for ceftiofur was 0.002 mg/kg; the accuracy (expressed as the average recoveries) was 80.6-105%; the precision (expressed as the coefficient of variation) was below 6.3% at 0.015, 0.03, and 0.06 mg/kg. The validated method demonstrated high accuracy and acceptable sensitivity to meet the Codex guideline requirements. The developed method was tested using market samples. As a results, ceftiofur was detected in one sample. Therefore, it can be applied to the analysis of ceftiofur residues in fishery products.

• 한국의류산업학회지
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• 제23권6호
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• pp.844-851
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• 2021

Due to the increase in consumers' interest about well-being, interest in eco-friendly products has been increasing due to the harmful effects of various harmful substances contained in textile products and environmental issues. As a result, natural dyes of less potential risk than synthetic dyes and digital textile printing(DTP) textile products with less environmental pollution are drawing attention. However, due to the lack of evaluation criteria for DTP textile products with natural ink and the nature of many colors are stacked layer by layer for dying, the need for simultaneous analysis is emerging. To evaluate whether the natural dye is derived from natural ingredients, the biocarbon content is analyzed. However, in the case of ink made using natural dyes and DTP textile products using natural ink, it is difficult to analyze the biocarbon content due to the limitation of the presence of a small amount of dye contained therein. In this study, we were shown the possibility of natural derived verification by cross-checking the analytes of natural dyes (Persicaria tinctoria, an indigo dye; Dactylopius coccus, a light red; and Curcum longa L., i.e., turmeric) and natural ink with HPLC-MS/MS. The coefficient of determination was 0.999 or higher, the limit of quantification was 0.647-3.664 ㎍/L and a %RSD of each indicator material was less than 10. Then, the extraction amount of natural dyes for five patterned fabrics was analyzed.

• Structural Engineering and Mechanics
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• 제78권3호
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• pp.351-368
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• 2021

This paper, by applying a reliability-based framework, develops seismic vulnerability macrozonation maps for Tehran, the capital and one of the most earthquake-vulnerable city of Iran. Seismic performance assessment of 3-, 4- and 5-story steel moment resisting frames (SMRFs), designed according to ASCE/SEI 41-17 and Iranian Code of Practice for Seismic Resistant Design of Buildings (2800 Standard), is investigated in terms of overall maximum inter-story drift ratio (MIDR) and unit repair cost ratio which is hereafter known as "damage ratio". To this end, Tehran city is first meshed into a network of 66 points to numerically locate low- to mid-rise SMRFs. Active faults around Tehran are next modeled explicitly. Two different combination of faults, based on available seismological data, are then developed to explore the impact of choosing a proper seismic scenario. In addition, soil effect is exclusively addressed. After building analytical models, reliability methods in combination with structure-specific probabilistic models are applied to predict demand and damage ratio of structures in a cost-effective paradigm. Due to capability of proposed methodology incorporating both aleatory and epistemic uncertainties explicitly, this framework which is centered on the regional demand and damage ratio estimation via structure-specific characteristics can efficiently pave the way for decision makers to find the most vulnerable area in a regional scale. This technical basis can also be adapted to any other structures which the demand and/or damage ratio prediction models are developed.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.405-405
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• 2023

최근 기후변화로 인한 극심한 홍수와 가뭄, 폭염 등 이상 기온 및 기후에 따른 피해가 급격히 증가하는 추세이다. 특히, 집중호우로 인해 도심지에서 발생한 홍수피해는 재산피해뿐만 아닌 수 많은 인명피해가 발생하고 있다. 국내에서는 수재해로부터 인명과 재산을 보호하기 위해 용수공급과 체계적인 치수 사업이 행한 바 있다. 이러한 치수 사업에서 경제성 분석은 사업 전·후 의 편익추정이 완벽히 검증되기 쉽지 않으며, 기존의 치수 계획은 유역 전반에 걸쳐 다양한 홍수 방어시설의 종합적인 고려 없이 제방 중심으로만 수립되어 홍수 발생 시 하천에 과도한 부담을 줄 뿐만 아니라, 사업의 경제성이 낮게 평가되고 있다. 이렇게 국내에서 행해진 하천설계기준에서 제안되었던 경제성 분석 방법은 여러 가지 문제점들을 내포하고 있으며 지속적인 홍수피해가 발생하였다. 이와 같은 문제점들을 개선하고자 개발된 K-FRM(Forean-Flood-Risk Model)은 능동형 하천정보 운영을 통한 다차원 하천관리체계 구축 및 활용을 위한 정량적 위험도 평가 툴로 위험지역에 노출된 자산의 정보(인벤토리), 평가기준, 손상함수, 계량화 원단위 등 홍수피해를 추정하는데 활용이 가능하며, 손실 또는 피해액으로 표현되는 재해손실은 경제적인 관점에서의 위험이며 금전적인 형태로 표현된다. 본 연구에서는 홍수피해액 정량화 모델인 K-FRM을 활용하여 굴포천 유역과 영강 유역을 대상으로 항목별 피해액 산정 후, 정량적 피해액 산정 방법인 EAD(Expected Annual Damage) 분석을 통해 홍수피해위험지도를 작성하여 표준유역별, 행정구역별 홍수피해액을 산정하는 것을 목적으로 한다.

• 한국재난정보학회 논문집
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• 제19권2호
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• pp.372-382
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• 2023

연구목적: 중대재해처벌법 시행에 따른 건설사 안전 관리 시스템 개선 방안을 제시한다. 연구방법: H건설사에서 이행 중인 중대 재해 처벌 법 대응 방안에 대한 안전보건관리체계를 안전보건진단을 통해 실무중심의 재해 예방활동의 효과를 분석하였다. 연구결과: 중대재해처벌법과 안전보건경영시스템의 연계로 재해율 감소 효과 및 안전보건경영 체계의 고도화 등이 분석되었고 중대재해처벌법 시행 전·후의 안전 활동 종합결과 분석을 통한 안전보건경영시스템 개선방안을 제시하였다. 결론: 중대재해처벌법 시행에 따른 사업주의 의식 개선, 재해예방을 위한 투자 확대, 재해예방활동의 정량화 등을 통한 실질적인 성과측정의 효과가 발현되었다.

• 한국환경농학회지
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• 제42권2호
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• pp.93-103
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• 2023

Persistence and degradation patterns of acequinocyl and its metabolite, hydroxyl-acequinocyl (acequinocyl-OH) and fenpyroximate in butterburs (Petasites japonicus Max.) were investigated after pesticide application. Butterburs, one of the minor crops in South Korea, was planted in two plots (plot A for double and plot B for single application) in a greenhouse. Butterburs samples were also planted in a separate plot without pesticide treatment, as the control. A commercial pesticide containing acequinocyl and fenpyroximate was applied to the foliage of butterburs at hourly intervals after dilution. Recoveries of acequinocyl and acequinocyl-OH were 78.6-84.7% and 83.7-95.5%, respectively; the relative standard deviation of the two compounds were less than 5%. The method limit of quantification was 0.01 mg/kg. The total (Ʃ) acequinocyl residues in butterburs reduced by 96.0% at 14 days and 75.9% at 7 days, in plot A and B, respectively, after final pesticide applications. The biological half-life (DT₅₀) of Ʃ acequinocyl and fenpyroximate, calculated using the dissipation rate, was 3.0 days and 4.0 days, respectively. These data were used to set up maximum residue and safe standard levels when the pesticides are applied to control pests during butterbur cultivation. Risk assessment results showed that the maximum % acceptable daily intake was 7.74% for Ʃ acequinocyl and 0.16% for Ʃ fenpyroximate. The theoretical maximum daily intake of Ʃ acequinocyl and fenpyroximate was 26.3% and 35.8%, respectively. In conclusion, the concentrations of Ʃ acequinocyl and fenpyroximate in butterburs pose no significant health risks to Koreans.

• Journal of Microbiology and Biotechnology
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• 제33권8호
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• pp.1101-1110
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• 2023

Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2-2.2 × 10⁶ colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.